CN-121977975-A - Method and system for detecting oxidation potential of atmospheric particulates

Abstract

The invention relates to the technical field of chemical detection, in particular to a detection method and a detection system for atmospheric particulate oxidation potential, wherein the detection method comprises the steps of injecting a reaction solution obtained by mixing and incubating a probe molecule solution and an atmospheric particulate solution into a photoelectrochemical microfluidic chip of a three-electrode system; substituting the obtained photocurrent response value into a pre-established standard curve to obtain an equivalent value of an oxidizing component in the reaction liquid, thereby realizing the detection of the oxidation potential of the atmospheric particulates. The photoelectrochemical sensor is combined with the microfluidic system to form a photoelectrochemical microfluidic chip, so that a high-efficiency analysis system is constructed. The oxidizing components in the atmospheric particulates are utilized to specifically react with the probe molecules, so that the probe molecules are consumed or reactants are generated, and quantitative change of photocurrent signals is further caused, so that the oxidizing potential of the atmospheric particulates is indirectly represented by detecting the consumption of the probe molecules, and the interference of complex matrixes on direct detection is effectively avoided.

Inventors

• ZHANG BINTIAN
• HU YA

Assignees

• 南方科技大学

Dates

Publication Date

20260505

Application Date

20251211

Claims (10)

1. 1. The method for detecting the oxidation potential of the atmospheric particulates is characterized by comprising the following steps: Mixing the probe molecule solution with the atmospheric particulate solution, and incubating to obtain a reaction solution; injecting the reaction liquid into a photoelectrochemical microfluidic chip of a three-electrode system to obtain a photocurrent response value, wherein a working electrode in the three-electrode system is modified with a photoelectroactive material film; Substituting the photocurrent response value into a pre-established standard curve to obtain an equivalent value of an oxidizing component in the reaction liquid, and detecting the oxidation potential of the atmospheric particulates.
2. 2. The method according to claim 1, wherein the probe molecules in the probe molecule solution are one or more selected from the group consisting of electron donor type probes, electron acceptor type probes, and enzyme-catalyzed reaction type probes.
3. 3. The method for detecting the oxidation potential of atmospheric particulates according to claim 2, wherein the probe molecule is selected from one or more of dithiothreitol, glutathione, cysteine, ascorbic acid, dopamine, potassium ferricyanide/potassium ferrocyanide, methylene blue, horseradish peroxidase-hydrogen peroxide system, superoxide dismutase, and catalase.
4. 4. The method for detecting the oxidation potential of an atmospheric particulate according to claim 1, wherein the concentration of the probe molecule solution is 1mM-3mM, and the volume ratio of the probe molecule solution to the atmospheric particulate solution is (4-8): 1.
5. 5. The method for detecting the oxidation potential of atmospheric particulates according to claim 1, wherein the temperature of the incubation treatment is 20 ℃ to 40 ℃, and the time of the incubation treatment is 10min to 60min.
6. 6. The method of claim 1, wherein the material of the photovoltaic active material film is selected from one or more of CdIn 2 S 4 -MoS 2 heterojunction 、TiO 2 、ZnO、BiVO 4 、CdS、In 2 S 3 、CuInS 2 、g-C 3 N 4 、CsPbBr 3 、BP.
7. 7. The method for detecting the oxidation potential of atmospheric particulates according to claim 1, wherein the method for establishing the pre-established standard curve comprises the steps of: mixing quantitative probe molecule solution with standard oxidant solution with gradient concentration, and incubating to obtain a series of reaction solutions; injecting the series of reaction liquids into a photoelectrochemical microfluidic chip of a three-electrode system to obtain a series of photocurrent response values; and establishing a linear relation between the standard oxidant solution with gradient concentration and the serial photocurrent response value as a standard curve.
8. 8. A detection system for the oxidation potential of atmospheric particulates for implementing the detection method according to any one of claims 1 to 7, comprising: The photoelectrochemistry microfluidic chip comprises a polymer substrate, a transparent conductive substrate, a working electrode, a counter electrode and a reference electrode, wherein a microfluidic channel is formed in the surface of the polymer substrate, and the transparent conductive substrate is arranged on the polymer substrate and positioned on one side of the microfluidic channel; the data processing module comprises a data acquisition unit and a data processing unit, wherein the data acquisition unit is electrically connected with the working electrode, the counter electrode and the reference electrode respectively and is used for acquiring a photocurrent response value, the data processing unit is electrically connected with the data acquisition unit, and the data processing unit is used for substituting the photocurrent response value into a pre-established standard curve to obtain an equivalent value of an oxidizing component in the reaction liquid.
9. 9. The system for detecting the oxidation potential of atmospheric particulates according to claim 8, wherein the thickness of the film of the electro-optically active material is 0.1mm to 0.5mm.
10. 10. The system for detecting the oxidation potential of the atmospheric particulates according to claim 8, further comprising a conveying module, wherein the conveying module is connected with the micro-channel through a pipeline and is used for conveying the liquid to be detected into the micro-fluidic channel.

Description

Method and system for detecting oxidation potential of atmospheric particulates Technical Field The invention relates to the technical field of chemical detection, in particular to a detection method and a detection system for atmospheric particulate oxidation potential. Background Atmospheric particulates (Particulate Matters, PM) act as a complex mixture whose size and chemical composition dynamically vary with region, season and emissions source, resulting in significant differences in toxic effects. Epidemiological studies prove that PM exposure is closely related to respiratory/cardiovascular diseases, and the core pathogenic mechanism is to induce oxidative stress, namely, after components such as polycyclic aromatic hydrocarbon, heavy metal and the like carried by particles penetrate through alveolar barriers, active oxygen (Reactive Oxygen Species, ROS) is produced through catalysis, so that the oxidation-antioxidation balance in cells is destroyed, and inflammation and deoxyribonucleic acid (Deoxyribonucleic acid, DNA) damage are induced. The evaluation of the toxicity effect of the atmospheric particulates is a key link for measuring the health risk of the population, and the traditional evaluation method taking the mass concentration as an index has fundamental limitation that heterogeneity of toxicity of different components cannot be distinguished, so that the contradiction frequency of the research result of the health effect is high. Oxidation potential (Oxidative Potential, OP) has emerged as a novel exposure indicator that directly characterizes PM's ability to induce oxidative stress, integrating chemical composition, surface reactivity and multicomponent synergistic effects, with a significant better correlation to healthy endpoints than mass concentrations, being the core parameter for toxicity assessment. The existing OP detection technology is mainly divided into two types, namely a bioanalytical method and a chemical instrument method. The oxidation potential of the atmospheric particulates is a key index for evaluating the health risk of the atmospheric particulates, and the traditional detection methods such as ultraviolet spectrophotometry and high performance liquid chromatography have the limitations of complex operation flow, long analysis period, high equipment cost, easy interference by sample matrixes and the like, and are difficult to meet the requirements of on-site rapid detection and large-scale sample screening. Accordingly, the prior art is still in need of improvement and development. Disclosure of Invention In view of the shortcomings of the prior art, the invention aims to provide a detection method and a detection system for the oxidation potential of atmospheric particulates, and aims to solve the problems of complex operation and insufficient sensitivity of the existing detection method. The technical scheme of the invention is as follows: A method for detecting the oxidation potential of atmospheric particulates comprises the following steps: Mixing the probe molecule solution with the atmospheric particulate solution, and incubating to obtain a reaction solution; injecting the reaction liquid into a photoelectrochemical microfluidic chip of a three-electrode system to obtain a photocurrent response value, wherein a working electrode in the three-electrode system is modified with a photoelectroactive material film; Substituting the photocurrent response value into a pre-established standard curve to obtain an equivalent value of an oxidizing component in the reaction liquid, and detecting the oxidation potential of the atmospheric particulates. The method for detecting the oxidation potential of the atmospheric particulates comprises the step of selecting one or more of an electron donor type probe, an electron acceptor type probe and an enzyme catalytic reaction type probe from probe molecule solutions. The detection method of the oxidation potential of the atmospheric particulates comprises the step of selecting one or more of dithiothreitol, glutathione, cysteine, ascorbic acid, dopamine, potassium ferricyanide/potassium ferrocyanide, methylene blue, horseradish peroxidase-hydrogen peroxide (HRP-H 2O2) system, superoxide dismutase (SOD) and Catalase (CAT). The method for detecting the oxidation potential of the atmospheric particulates comprises the steps of enabling the concentration of the probe molecule solution to be 1mM-3mM, and enabling the volume ratio of the probe molecule solution to the atmospheric particulates solution to be (4-8): 1. According to the method for detecting the oxidation potential of the atmospheric particulates, the temperature of the incubation treatment is 20-40 ℃, and the incubation treatment time is 10-60 min. The method for detecting the oxidation potential of the atmospheric particulates comprises the step of selecting one or more materials of the photoelectric active material film from CdIn 2S4-MoS2 heterojunction 、TiO2、ZnO、Bi