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CN-121978030-A - Imported sea fish pathogenic parasite sampling and detecting device

CN121978030ACN 121978030 ACN121978030 ACN 121978030ACN-121978030-A

Abstract

The invention belongs to the technical field of parasite sampling, in particular to a sampling and detecting device for pathogenic parasites of imported sea fish, which aims at solving the problems of low manual sampling efficiency, inaccurate positioning and easy damage of samples in the existing sea fish parasite quarantine, the proposal is that the device comprises a sampling base, a movable supporting plate, a sampling area identifying component, a sampling tube and a negative pressure separating component. The sampling area identification component can nondestructively and accurately locate the suspected parasite infected area in the fish body. The sampling tube is provided with an annular air guide cavity and an annular liquid guide cavity, and sterile gas and normal saline can be cooperatively sprayed, so that the minimally invasive, efficient stripping and recovery of the insect bodies are realized. The negative pressure separation assembly adopts a double-chamber design, a micro-filtration membrane is arranged in the negative pressure separation assembly, a sample can be split and independently treated in a single operation, and the negative pressure liquid suction pipe has an automatic valve function of negative pressure communication and normal pressure closing, so that the subsequent in-situ fixation is facilitated. The invention realizes full-flow automation from accurate minimally invasive sampling to automatic separation and fixation.

Inventors

  • LIU BO
  • LIU JING
  • ZHANG MINAI
  • WANG SUQIU

Assignees

  • 长春海关技术中心

Dates

Publication Date
20260505
Application Date
20260213

Claims (10)

  1. 1. The sampling detection device for the pathogenic parasites of the imported sea fish comprises a sampling base (1) and is characterized in that a sampling pipe (5) and a negative pressure separation assembly (6) which are communicated are arranged on the sampling base (1), a supporting plate (2) is movably arranged on the sampling base (1), and a sampling area identification assembly is arranged right above a moving path of the supporting plate (2); the sampling area identification component comprises a light curtain sensor (31), a near infrared light source (32) and a multispectral camera (33); The pipe body of the sampling pipe (5) is sequentially provided with a first reducing pipe body (51), a second reducing pipe body (52) and a puncture needle head (53), an annular air guide cavity (57) is formed at the joint part of the sampling pipe (5) and the first reducing pipe body (51), air in the annular air guide cavity (57) is introduced into a sampling area, an annular liquid guide cavity (59) is formed at the joint part of the first reducing pipe body (51) and the second reducing pipe body (52), and liquid in the annular liquid guide cavity (59) is introduced into the sampling area; Negative pressure separation subassembly (6) are including filter tube (63), micro-filtration pipe (64) and collector tube (65) that top-down set gradually, be equipped with filter screen (615) in filter tube (63), be equipped with two independent cavities in micro-filtration pipe (64), and all settle in every cavity has micro-filtration membrane (618), every cavity bottom of micro-filtration pipe (64) all communicates has negative pressure drawing liquid pipe (619), negative pressure drawing liquid pipe (619) is the passageway state when being in the negative pressure, negative pressure drawing liquid pipe (619) is the closed circuit state when being in the ordinary pressure.
  2. 2. The device for sampling and detecting pathogenic parasites of imported sea fish according to claim 1, wherein the seat body of the sampling base (1) is provided with a long limiting chute (11), the bottom surface of the supporting plate (2) is connected with a sliding block (22), the sliding block (22) is slidably arranged in the limiting chute (11), a driving motor (12) is embedded in a chute wall on one side of the limiting chute (11), a screw rod (13) is fixedly connected with a motor shaft coaxial line of the driving motor (12), a shaft thread of the screw rod (13) penetrates through the sliding block (22), and a plurality of balls (21) are embedded in two sides of the bottom surface of the supporting plate (2).
  3. 3. The device for detecting pathogenic parasites of imported sea fish, as set forth in claim 1, wherein the sampling area identifying component further comprises an identifying outer cover (3), the light curtain sensor (31), the near infrared light source (32) and the multispectral camera (33) are all integrally installed on the inner top surface of the cover of the identifying outer cover (3), the near infrared light source (32) is arranged in a ring shape, the installation position of the multispectral camera (33) is located at the center of the near infrared light source (32), notches are formed in two sides of the identifying outer cover (3), the plate body of the supporting plate (2) is U-shaped, and when the supporting plate (2) moves to the position right below the identifying outer cover (3), the two side plate bodies of the supporting plate (2) are clamped at the notch parts formed in the cover body of the identifying outer cover (3).
  4. 4. The sampling detection device for pathogenic parasites of import sea fish according to claim 1, wherein the six-axis mechanical arm (4) is installed on the sampling base (1), the installation position of the six-axis mechanical arm (4) is located at one side of the moving path of the supporting plate (2), and the sampling tube (5) is clamped and fixed at the movable front end of the six-axis mechanical arm (4).
  5. 5. The device for sampling and detecting pathogenic parasites of sea fish in the inlet of claim 1, wherein the upper end of the pipe body of the sampling pipe (5) is connected with an air inlet joint (54) and a liquid inlet joint (55), a liquid conveying channel (58) communicated between the liquid inlet joint (55) and an annular liquid guide cavity (59) is formed in the pipe body of the sampling pipe (5), a plurality of annular equally-spaced liquid through micropores (511) are formed in the butt joint inclined surface part of the sampling pipe (5) and the first reducing pipe body (51), a plurality of annular equally-spaced liquid through micropores (511) are communicated with the annular liquid guide cavity (59), a gas conveying channel (56) communicated between the air inlet joint (54) and the annular liquid guide cavity (57) is formed in the pipe body of the sampling pipe (5) and the pipe body of the first reducing pipe body (51), a plurality of annular equally-spaced air through micropores (510) are formed in the butt joint inclined surface part of the first reducing pipe body (51) and the second reducing pipe body (52), and the plurality of annular equally-spaced air through micropores (510) are communicated with the annular air guide cavity (57).
  6. 6. The device for sampling and detecting pathogenic parasites in sea fish, as set forth in claim 1, wherein the negative pressure separation assembly (6) further comprises an upper cover (62) disposed at the top end of the filter tube (63), a transfusion tube (7) is communicated between the upper cover (62) and the sampling tube (5), the upper cover (62), the filter tube (63), the micro-filtration tube (64) and the liquid collecting tube (65) are in threaded butt joint, and a sealing rubber ring (8) is sleeved at the butt joint part of each tube.
  7. 7. The device for sampling and detecting pathogenic parasites of imported sea fish according to claim 6, wherein the negative pressure separation assembly (6) further comprises a positioning supporting plate (61), supporting rods (66) which are vertically arranged are fixedly connected between two ends of the positioning supporting plate (61) and the sampling base (1), limiting grooves (69) are formed in the middle of the body of the positioning supporting plate (61), positioning grooves (610) are formed in two sides of the limiting grooves (69), a limiting ring (67) and a positioning clamping block (68) are fixedly connected to the pipe body of the filter pipe (63), the positioning clamping block (68) is located below the limiting ring (67), when the pipe body of the filter pipe (63) is inserted into the limiting grooves (69), the limiting ring (67) is clamped above the limiting grooves (69), and the positioning clamping block (68) is clamped in the positioning grooves (610).
  8. 8. The device for sampling and detecting pathogenic parasites of sea fish in inlet of claim 7, wherein the inner wall of the filter tube (63) is movably clamped with a net body outer ring (614), the filter screen (615) is connected to the net body outer ring (614), a liquid collecting bottom plate (616) is fixedly connected to the inside of the micro-filter tube (64), a lower butt joint partition plate (620) is fixedly connected to the inside of the micro-filter tube (64) along the diameter direction, an upper butt joint partition plate (625) is fixedly connected to the lower butt joint partition plate (620), a slot (626) is formed in the bottom edge of the upper butt joint partition plate (625), the lower butt joint partition plate (620) is inserted into the slot (626) when the filter tube (63) and the micro-filter tube (64) are in a butt joint state, micro-filter bottom nets (617) are further connected to the inside of the micro-filter tube (64), the micro-filter bottom nets (617) are respectively located on two sides of the lower butt joint partition plate (620), and the micro-filter bottom nets (618) are paved on the micro-filter bottom nets (617).
  9. 9. The device for sampling and detecting pathogenic parasites of import sea fish according to claim 8, wherein the liquid collecting bottom plate (616) is communicated with two negative pressure liquid suction pipes (619), the two negative pressure liquid suction pipes (619) are respectively corresponding to two independent cavities separated by a lower butt joint partition plate (620), an inner cylinder (621) is movably connected in the pipe of the negative pressure liquid suction pipes (619), a clamping ring (622) is connected to the barrel body of the inner cylinder (621), an inner concave clamping groove (627) is formed in the inner wall of the pipe of the negative pressure liquid suction pipe (619), the clamping ring (622) is clamped in the inner concave clamping groove (627), a compression spring (623) is sleeved on the barrel body of the inner cylinder (621), two ends of the compression spring (623) are respectively abutted between the clamping ring (622) and the bottom surface of the inner concave clamping groove (627), a liquid drain port (619) is formed in the barrel body at the lower end of the inner cylinder (621), and the bottom surface of the inner cylinder (621) and the bottom of the negative pressure liquid suction pipe (627) are in the same plane in the initial state.
  10. 10. The device for sampling and detecting pathogenic parasites in sea fish, as set forth in claim 9, characterized in that the bottom end of the micro-filtration tube (64) is connected with the liquid collection tube (65) when the micro-filtration tube (64) is in a negative pressure separation sampling state, a negative pressure joint (611) is communicated between the liquid collection tube (65) and an external negative pressure pump, the negative pressure joint (611) is connected to the upper end side wall of the liquid collection tube (65), the bottom end of the micro-filtration tube (64) is connected with the movable bottom cover (612) when the micro-filtration tube (64) is in a sampling completion state, a supporting block (613) is connected to the movable bottom cover (612), and the supporting block (613) is supported below the negative pressure liquid suction tube (619) when the micro-filtration tube (64) is in butt joint with the movable bottom cover (612).

Description

Imported sea fish pathogenic parasite sampling and detecting device Technical Field The invention relates to a sampling and detecting device, in particular to a sampling and detecting device for pathogenic parasites of imported sea fish, and belongs to the technical field of parasite sampling. Background With the advancement of global trade integration and the initiative of 'one-way-one', the quantity of imported aquatic products in China, especially frozen pollack fish and other sea fish from the Russian far east and North Pacific ocean sea areas, is continuously increased. Such sea fish are common hosts of food-borne parasites such as heterodera, and the human beings can cause heterodera by eating contaminated fish meat by mistake, thereby seriously threatening public health and trade safety. Therefore, it is important to efficiently and accurately quarantine parasites of the inbound sea fish. However, the prior art system has a series of bottlenecks in the whole process of detecting and sampling, and is difficult to meet the requirements of quick clearance and accurate prevention and control of ports. Existing parasite detection techniques can be largely divided into laboratory detection and preliminary screening, but have obvious limitations. In the aspect of laboratory detection, as disclosed in the publication No. CN101603964A, the ELISA detection method for the heterodera in the seafood is characterized in that a specific antibody is prepared, and an ELISA method is adopted to detect the worm antigen in a sample, and the method has high sensitivity but is an off-line and destructive biochemical detection method. The method is seriously dependent on the prior manual process of splitting, separating and crushing the worm body from the fish body to obtain the antigen, the process itself has the problem of efficiently and accurately finding and obtaining the parasite sample deeply buried in the muscle or abdominal cavity of the fish body, and the TAQMANPCR detection method of the simple heterodera in the marine product disclosed by the publication No. 103361401A requires a high-quality worm body or DNA sample as input, and the traditional sampling means is the weakest link in the chain and cannot be automated. At present, port quarantine mainly depends on manual visual inspection and dissection, and the method has extremely low efficiency, high labor intensity, obvious influence by personnel experience and extremely easy omission. Furthermore, how to pre-treat the mixed suspension containing the worms rapidly and in parallel (e.g. to fix the differences for different subsequent detection purposes) after obtaining it also lacks an integrated solution. The existing device has single function, rough sampling process often causes damage to the insects or sample pollution, and can not realize 'one-taking-multiple-standby' so as to meet various subsequent analysis requirements such as morphological identification and molecular biological detection. Disclosure of Invention The invention provides a sampling and detecting device for pathogenic parasites of imported sea fish, which aims to solve the technical problems of low manual sampling efficiency, inaccurate positioning and easy sample damage in the conventional sea fish parasite quarantine. The invention realizes the purpose through the following technical scheme that the sampling detection device for pathogenic parasites of imported sea fish comprises a sampling base, wherein a sampling tube and a negative pressure separation assembly which are communicated are arranged on the sampling base, a supporting plate is movably arranged on the sampling base, and a sampling area identification assembly is arranged right above a supporting plate moving path; the sampling area identification component comprises a light curtain sensor, a near infrared light source and a multispectral camera; The pipe body of the sampling pipe is sequentially provided with a first reducing pipe body, a second reducing pipe body and a puncture needle head, the joint part of the sampling pipe and the first reducing pipe body is provided with an annular air guide cavity, air flow in the annular air guide cavity is introduced into a sampling area, the joint part of the first reducing pipe body and the second reducing pipe body is provided with an annular liquid guide cavity, and liquid in the annular liquid guide cavity is introduced into the sampling area; The negative pressure separation assembly comprises a filter pipe, a micro-filter pipe and a liquid collecting pipe which are sequentially arranged from top to bottom, a filter screen is arranged in the filter pipe, two independent cavities are arranged in the micro-filter pipe, micro-filter membranes are arranged in each cavity, the bottom end of each cavity of the micro-filter pipe is communicated with a negative pressure liquid suction pipe, the negative pressure liquid suction pipe is in a passage state when in negative pressure, an