CN-121978073-A - Method and device for reading immunochromatography detection result based on solid-state quantum enhancement

CN121978073ACN 121978073 ACN121978073 ACN 121978073ACN-121978073-A

Abstract

The application provides an immunochromatography detection result reading method and device based on solid-state quantum enhancement, and relates to the technical field of immunochromatography detection. The immunochromatography detection result reading method comprises the steps of emitting excitation light to a standard substance to excite the standard substance to generate a fluorescence signal for detection, outputting microwaves to the standard substance to enable the standard substance to generate spin resonance and cause intensity change of the fluorescence signal, collecting the fluorescence signal after the intensity change to obtain a photodetection magnetic resonance ODMR reference spectral line, determining corresponding microwave calibration frequency according to the photodetection magnetic resonance ODMR reference spectral line, emitting the excitation light and microwaves which are calibrated according to the microwave calibration frequency and are subjected to amplitude or frequency modulation to a detected sample, and respectively detecting fluorescence intensity change amounts of a quality control line and a detection line to obtain an immunochromatography detection result of the detected sample. The application realizes the calibration of microwave frequency by using the standard substance, effectively shields background fluorescence interference and improves the detection sensitivity and accuracy.

Inventors

YUE CHENGLIANG
LIU YAN
YANG RENFU

Assignees

北京量子信息科学研究院

Dates

Publication Date: 20260505
Application Date: 20260408

Claims (9)

1. The immunochromatography detection result reading method based on solid-state quantum enhancement is characterized by comprising the following steps of: Emitting excitation light to a standard substance to excite the standard substance to generate a fluorescent signal for detection; outputting microwaves to the standard substance, so that the standard substance generates spin resonance and causes the intensity of the fluorescence signal to change; Collecting the fluorescence signal after the intensity change to obtain a reference spectrum line of the optical detection magnetic resonance ODMR; determining a corresponding microwave calibration frequency according to the optical detection magnetic resonance ODMR reference spectral line; transmitting the excitation light and microwaves calibrated according to the microwave calibration frequency to a quality control line and a detection line of a tested sample; And respectively detecting the fluorescence intensity variation of the quality control line and the fluorescence intensity variation of the detection line to obtain an immunochromatography detection result of the detected sample.
2. The immunochromatographic assay result reading method of claim 1, wherein the standard substance comprises a diamond NV color center standard substance.
3. The immunochromatographic assay result reading method of claim 1, wherein the outputting of microwaves to the standard substance, causing spin resonance of the standard substance, and causing a change in the intensity of the fluorescent signal, comprises: and outputting microwaves by adopting a triangular wave or saw tooth wave sweep frequency method.
4. The immunochromatographic assay result reading method of claim 1, wherein the emitting of the excitation light and the microwaves calibrated according to the microwave calibration frequency to the quality control line and the detection line of the sample to be tested comprises: And performing amplitude modulation or frequency modulation on microwaves calibrated according to the microwave calibration frequency so as to enable the fluorescent signals of the quality control line and the detection line to generate intensity variation.
5. The immunochromatographic assay result reading method according to claim 1, wherein the detecting the amounts of change in fluorescence intensity of the quality control line and the detection line, respectively, to obtain the immunochromatographic assay result of the sample to be tested, comprises: When the fluorescence intensity variation of the quality control line reaches a first set threshold value and the fluorescence intensity variation of the detection line reaches a second set threshold value, determining that the immunochromatography detection result of the detected sample is positive; when the fluorescence intensity variation of the quality control line reaches the first set threshold value and the fluorescence intensity variation of the detection line does not reach the second set threshold value, determining that an immunochromatography detection result of the detected sample is negative; and under the condition that the fluorescence intensity variation of the quality control line does not reach the first set threshold, determining that the immunochromatography operation fails.
6. An immunochromatographic detection result reading device based on solid-state quantum enhancement, which is used for executing the immunochromatographic detection result reading method according to any one of claims 1 to 5, the immunochromatographic detection result reading device comprising: the laser is used for emitting excitation light to the standard substance so as to excite the standard substance to generate a fluorescence signal for detection; a microwave component for outputting microwaves to the standard substance, causing the standard substance to generate spin resonance, and causing an intensity change of the fluorescent signal; the fluorescence acquisition component is used for acquiring the fluorescence signal after the intensity change; The control assembly is respectively connected with the laser, the microwave assembly and the fluorescence acquisition assembly, and is used for determining a photodetection magnetic resonance ODMR reference spectral line according to a fluorescence signal acquired by the fluorescence acquisition assembly, determining a corresponding microwave calibration frequency according to the photodetection magnetic resonance ODMR reference spectral line, and controlling the laser to respectively emit the excitation light to a quality control line and a detection line of a tested sample, and controlling the microwave assembly to respectively emit microwaves calibrated according to the microwave calibration frequency to the quality control line and the detection line of the tested sample; The phase locking assembly is respectively connected with the fluorescence acquisition assembly and the control assembly and is used for extracting the fluorescence intensity variation of the quality control line and the detection line according to the fluorescence signals of the quality control line and the detection line acquired by the fluorescence acquisition assembly, and the control assembly is also used for respectively detecting the fluorescence intensity variation of the quality control line and the detection line to obtain an immunochromatography detection result of the detected sample; The bracket is used for bearing the standard substance and the tested sample; And the displacement assembly is respectively connected with the bracket and the control assembly and used for receiving the control of the control assembly so as to move the bracket, so that the laser and the microwave assembly are sequentially aligned with the standard substance, the quality control line and the detection line on the tested sample.
7. The immunochromatographic assay result reading device of claim 6, wherein the microwave component is configured to: And when the standard substance is detected, outputting microwaves by adopting a triangular wave or saw tooth wave sweep frequency method.
8. The immunochromatographic assay result reading device of claim 6, wherein the microwave component is configured to: And when the quality control line and the detection line are detected, performing amplitude modulation or frequency modulation on microwaves calibrated according to the microwave calibration frequency so as to enable fluorescent signals of the quality control line and the detection line to generate intensity changes.
9. The immunochromatographic assay result reading device of claim 6, further comprising: and the interaction assembly is connected with the control assembly and is used for receiving the detection instruction and transmitting the detection instruction to the control assembly.

Description

Method and device for reading immunochromatography detection result based on solid-state quantum enhancement Technical Field The application relates to the technical field of immunochromatography detection, in particular to a method and a device for reading an immunochromatography detection result based on solid-state quantum enhancement. Background Immunochromatography is an immunoassay technology for realizing rapid detection through antigen-antibody specific binding reaction, and the core mechanism is to combine an analyte in a sample with immobilized antigen/antibody by utilizing the chromatography effect to form visible or detectable signals so as to complete analysis. The conventional immunochromatography detection technology comprises a colloidal gold immunochromatography technology and a fluorescent immunochromatography technology, wherein the colloidal gold immunochromatography technology adopts colloidal gold particles as markers, qualitative detection is completed by observing color development results through naked eyes, the defects of poor sensitivity and difficulty in accurate quantification exist, the fluorescent immunochromatography technology realizes signal enhancement through the fluorescent markers, the detection sensitivity is improved to a certain extent compared with that of a colloidal gold method, but fluorescence is emitted by an immunochromatography carrier nitrocellulose membrane and a test paper cassette material after being stimulated, so that the acquired fluorescence intensity is not all from fluorescent substances to be detected, and the accuracy of the detection results is finally affected. In the prior art, a detection method based on a diamond Nitrogen Vacancy (NV) color center is also provided, the diamond NV color center is a solid quantum material with unique optical and spin characteristics, fluorescence is generated by exciting the NV color center and detection is realized by combining microwave resonance, the detection sensitivity is improved to a certain extent, but the method does not consider the influence of environmental factors such as a magnetic field, temperature and the like on the NV color center energy gap, the mismatch of the microwave frequency and the NV color center energy gap is easy to cause, further detection errors are generated, the integration and the suitability of a detection flow are poor, and the detection requirements of test strips with different specifications and multiple indexes are difficult to meet. Disclosure of Invention The application aims to provide an immunochromatography detection result reading method and device based on solid quantum enhancement, which utilize diamond NV color center standard substances to realize accurate calibration of microwave frequency, combine microwave amplitude modulation or frequency modulation and phase-locked detection technology, effectively shield background fluorescence interference, improve detection sensitivity and accuracy, and solve the technical problems that the existing immunochromatography detection technology is low in sensitivity, poor in quantitative effect and easy to be influenced by background fluorescence interference, and the existing quantum-based detection method is easy to be influenced by environmental factors and has large detection error. According to a first aspect of the application, at least one embodiment of the application provides an immunochromatography detection result reading method based on solid-state quantum enhancement, which comprises the steps of emitting excitation light to a standard substance to excite the standard substance to generate a fluorescence signal for detection, outputting microwaves to the standard substance to enable the standard substance to generate spin resonance and cause intensity change of the fluorescence signal, collecting the fluorescence signal after the intensity change to obtain a photodetection magnetic resonance ODMR reference spectral line, determining corresponding microwave calibration frequency according to the photodetection magnetic resonance ODMR reference spectral line, emitting the excitation light and microwaves calibrated according to the microwave calibration frequency to a quality control line and a detection line of a detected sample, and respectively detecting the fluorescence intensity change amounts of the quality control line and the detection line to obtain an immunochromatography detection result of the detected sample. For example, in some embodiments of the application, the standard comprises a diamond NV color center standard. For example, in some embodiments of the application, the outputting microwaves to the standard substance, causing the standard substance to generate spin resonance, and causing the intensity of the fluorescent signal to change, includes outputting microwaves using a triangle wave or saw tooth wave sweep method. For example, in some embodiments of the application, the emitting the exci