CN-121978092-A - Food trace element and animal residue integrated detection assembly and method combining enzymatic reaction

CN121978092ACN 121978092 ACN121978092 ACN 121978092ACN-121978092-A

Abstract

The invention relates to the technical field of food safety detection, in particular to a food trace element and animal residue integrated detection assembly and method combining enzymatic reaction, comprising a sample storage module and a liquid pumping module, wherein the liquid pumping module pumps liquid through a micro pump; the enzymatic reaction module comprises a substrate and a pair of mutually independent reaction units, wherein the substrate is communicated and connected with the sample storage module through a pipeline, the substrate is also communicated and connected with the pair of reaction units, and the two reaction units comprise a first immobilized enzyme aiming at heavy metal ions, a first reaction system for developing enzyme reaction substrates, a second immobilized enzyme aiming at veterinary drug residues and a second reaction system for developing enzyme reaction substrates. According to the invention, the first type reaction unit aiming at heavy metal ions and the second type reaction unit aiming at veterinary drug residues are integrated on the liquid outlet side of the same microfluidic substrate, and the microfluidic channel network is utilized to realize one-time sample adding and synchronous distribution of the liquid to be detected, so that the high integration and synchronization of the detection flow are realized, and the detection efficiency is improved.

Inventors

XU HUI
YU YUE
YANG PANPAN
GUO XIAOPENG
SHAO LEI
ZHANG XIAOYU

Assignees

青岛市食品药品检验研究院(青岛市纤维纺织品检验研究院、青岛市药品不良反应监测中心、青岛市实验动物和动物实验中心)

Dates

Publication Date: 20260505
Application Date: 20260123

Claims (10)

1. The integrated detection assembly for the trace elements and the animal residues of the food, which combines enzymatic reaction, is characterized by comprising a sample storage module, a detection module and a detection module, wherein the sample storage module is used for storing prepared liquid to be detected and pumping the liquid through a micro pump; The enzymatic reaction module comprises a substrate and a pair of mutually independent reaction units, wherein the substrate is communicated and connected with the sample storage module through a pipeline, and the substrate is also communicated and connected with the pair of reaction units, wherein one reaction unit is used as a first type reaction unit and comprises a first immobilized enzyme aiming at heavy metal ions and a first reaction system for developing an enzyme reaction substrate, and the other reaction unit is used as a second type reaction unit and comprises a second immobilized enzyme aiming at veterinary drug residues and a second reaction system for developing the enzyme reaction substrate; the signal acquisition module synchronously acquires signal changes of the reaction units due to enzymatic reactions by adopting image acquisition equipment; And the intelligent analysis module processes the acquired signals through a computer and outputs detection results of all the targets based on pre-stored data.
2. The integrated detection assembly for food trace elements and animal residues combined with enzymatic reactions according to claim 1 wherein the first immobilized enzyme is selected from at least one of urease, horseradish peroxidase, alkaline phosphatase, the heavy metal ions include at least one of lead, mercury, cadmium, arsenic, the second immobilized enzyme is selected from at least one of beta-lactamase, glucose oxidase, cholinesterase, acetylcholinesterase, cytochrome P450 enzymes, and the animal residues include at least one of antibiotics, hormones, beta-receptor agonists.
3. The integrated food trace element and animal residue detection assembly according to claim 2 wherein when the detectable signal is a color change, the first and second reaction systems comprise a substance that converts the corresponding enzymatic reaction to a color signal; for assays based on the principle of enzyme inhibition, the substance comprises a pH indicator or a peroxidase chromogenic substrate; For assays based on the principle of competitive inhibition or enzymatic direct hydrolysis, the material comprises a specific chromogenic substrate or a conjugated chromogenic reaction reagent.
4. The integrated detection assembly for trace elements and animal residues of foods combined with enzymatic reaction according to claim 3, wherein the substrate is a microfluidic chip and comprises a plurality of S-shaped microfluidic channels, the reaction unit is a reaction chamber box comprising a plurality of independent cavities, and a liquid passage is arranged between every two rows of the cavities in the reaction chamber box and is used for leading a liquid to be detected into the cavities by butting the microfluidic channels so as to react with the immobilized enzyme and the reaction system for color development.
5. The integrated detection assembly for food trace elements and animal residues, which combines enzymatic reaction according to claim 4, wherein the top surface of the reaction chamber box is open and is clamped with a sealing cover, the bottom surface of the sealing cover is provided with a plurality of sealing platforms in a side-by-side protruding mode, and the sealing platforms are in fit and clamping connection with the liquid passage.
6. The integrated detection assembly for food trace elements and animal residues combined with enzymatic reactions according to claim 5 wherein the intelligent analysis module is configured to identify and extract color development information in a plurality of cavities of the reaction chamber box in an image obtained by the image acquisition device, convert the color development information into signal variation values, compare the signal variation values with pre-stored data and analyze the signal variation values, and a standard curve database for enzymatic reactions with trace elements and animal residues is arranged in the intelligent analysis module.
7. The integrated detection assembly for trace elements and animal residues in food, which combines enzymatic reaction according to claim 6, wherein the top surface of the substrate is provided with a plurality of liquid inlets which are communicated with a plurality of microfluidic channel inlets, and the front side surface of the substrate is provided with a plurality of liquid outlets which are communicated with a plurality of microfluidic channel outlets.
8. The integrated detection assembly for trace elements and animal residues in food, which is combined with enzymatic reaction, according to claim 7, wherein one end face of the reaction chamber box is provided with liquid inlets communicated with a plurality of liquid channels, and the side wall of each liquid channel is provided with a liquid separation port communicated with a cavity adjacent to the reaction chamber box.
9. The integrated detection assembly for trace elements and animal residues in food, which is combined with enzymatic reaction, according to claim 8, wherein the enzymatic reaction module further comprises a base frame which is in fit and clamping connection with the base and a plurality of reaction units, the reaction units are arranged on the liquid outlet side of the base side by side, and a plurality of passage openings are formed in the side surface of a lining rod of the base frame on the liquid outlet side of the base in a penetrating manner and are used for communicating the liquid outlet and the liquid through openings.
10. An integrated detection method for food trace elements and animal residues combined with enzymatic reaction, which uses the integrated detection assembly for food trace elements and animal residues combined with enzymatic reaction according to claim 9, and is characterized by comprising the following steps: S1, pre-treating a food sample to be detected to obtain a liquid to be detected, and pouring the liquid to be detected into a sample storage module; S2, starting a micro pump arranged on the sample storage module, injecting the liquid to be detected into the substrate, flowing into all reaction units of the enzymatic reaction module, and incubating for 5 to 20 minutes at 25 to 40 ℃; S3, synchronously capturing enzymatic reaction and chromogenic reaction images generated by each reaction unit by starting a signal acquisition module, and changing signal values before and after incubation or in the incubation process; S4, receiving the signal change value through an intelligent analysis module, comparing and analyzing the signal change value with a pre-stored threshold value or a standard curve, and finally outputting whether one or more trace elements and veterinary drug residues exist in the sample.

Description

Food trace element and animal residue integrated detection assembly and method combining enzymatic reaction Technical Field The invention relates to the technical field of food safety detection, in particular to a food trace element and animal residue integrated detection assembly and method combining enzymatic reaction. Background The field of food safety detection is an increasingly urgent need for rapid and synchronous screening of trace harmful substances. In the prior art, detection of heavy metal elements and veterinary drug residues is mostly dependent on large-scale instruments such as atomic absorption spectrum, liquid chromatography-mass spectrometry and the like, and although the methods are high in precision, the flow is complex, the time consumption is long, the cost is high, the steps are usually needed to be carried out, and the supervision and production self-checking requirements of on-site rapid and integrated screening are difficult to meet. The patent application with the application number of CN202422755135.0 discloses an enzymatic hydrolysis device for fruit essence, which comprises a reaction tank body, a feed liquid storage tank, a standard alkali liquid storage tank, a standard acid liquid storage tank and an enzyme liquid storage tank, and improves the hydrolysis efficiency and the mixing uniformity by optimizing a stirring structure and a control mode, and by adopting an accurate temperature control system and a multilayer temperature sensor layout, the accurate control of the reaction temperature is realized, and the quality stability of products is improved. In the prior art, although some enzymatic reaction detection methods exist, the application of the detection methods is limited to single-class targets, and the detection device is low in integration level and limited in automation degree. Therefore, the prior art lacks an integrated rapid detection device which can highly integrate a plurality of enzymatic reaction systems aiming at different types of harmful substances and realize automatic sample adding, reaction, image acquisition and intelligent analysis. Disclosure of Invention In order to overcome the defects in the prior art, the invention aims to provide an integrated detection assembly and method for food trace elements and animal residues, which are combined with enzymatic reaction, so as to solve the problems in the prior art. In order to achieve the above purpose, in one aspect, the invention provides an integrated detection assembly for trace elements and animal residues of food combined with enzymatic reaction, which comprises a sample storage module for storing prepared liquid to be detected and pumping the liquid through a micro pump; The enzymatic reaction module comprises a substrate and a pair of mutually independent reaction units, wherein the substrate is communicated and connected with the sample storage module through a pipeline, and the substrate is also communicated and connected with the pair of reaction units, wherein one reaction unit is used as a first type reaction unit and comprises a first immobilized enzyme aiming at heavy metal ions and a first reaction system for developing an enzyme reaction substrate, and the other reaction unit is used as a second type reaction unit and comprises a second immobilized enzyme aiming at veterinary drug residues and a second reaction system for developing the enzyme reaction substrate; the signal acquisition module synchronously acquires signal changes of the reaction units due to enzymatic reactions by adopting image acquisition equipment; And the intelligent analysis module processes the acquired signals through a computer and outputs detection results of all the targets based on pre-stored data. As a further improvement of the technical scheme, the first immobilized enzyme is at least one selected from urease, horseradish peroxidase and alkaline phosphatase, the heavy metal ions comprise at least one selected from lead (Pb), mercury (Hg), cadmium (Cd) and arsenic (As), the second immobilized enzyme is at least one selected from beta-lactamase, glucose oxidase, cholinesterase, acetylcholinesterase and cytochrome P450 enzyme, and the veterinary drug residues comprise at least one selected from antibiotics, hormones and beta-receptor agonists. As a further improvement of the present technical solution, when the detectable signal is a color change, the first reaction system and the second reaction system comprise a substance that converts the corresponding enzymatic reaction into a color signal; for assays based on the principle of enzyme inhibition, the substance comprises a pH indicator or a peroxidase chromogenic substrate; For assays based on the principle of competitive inhibition or enzymatic direct hydrolysis, the material comprises a specific chromogenic substrate or a conjugated chromogenic reaction reagent. As a further improvement of the technical scheme, the substrate is a microfluidic chip and c