CN-121978097-A - Method and system for identifying morphology of fungi of traditional Chinese medicine

CN121978097ACN 121978097 ACN121978097 ACN 121978097ACN-121978097-A

Abstract

The invention relates to a method and a system for identifying the morphology of a traditional Chinese medicine fungus, and relates to the field of quality detection of traditional Chinese medicine, wherein the method comprises the steps of marking according to suspected fungus areas so as to determine the analysis position of a hypha curve; the method comprises the steps of obtaining local image information, analyzing the local image information to determine a reference hypha curve graph, determining medicinal material fixing parameters based on hypha curve analysis positions, wherein the medicinal material fixing parameters comprise medicinal material fixing coordinates and medicinal material fixing angles, generating a small ball impact scheme based on the medicinal material fixing parameters, wherein the small ball impact scheme comprises an impact point and an impact speed, executing small ball impact operation according to the small ball impact scheme, determining a current hypha curve graph after the small ball impact operation, determining a hypha curve variable graph based on the current hypha curve graph and the reference hypha curve graph, and outputting the hypha position based on the hypha curve variable graph. The invention has the effect of distinguishing the collision texture and true hypha on the surface of the traditional Chinese medicine, and avoiding the situation that the physical injury and the biological hypha are difficult to distinguish by manual treatment, so that the misjudgment rate is higher.

Inventors

NIE FULI
LV QIAN
ZHANG JIE
Qian Hanfeng
LI XIAOLI
LI YOUQIN
Qiu Xunye
WANG YANJUN
ZHANG JIALEI
WANG TIANCHENG
HU YU
MAO XIFENG
Jiang Yingxiu
YU JUNJUN

Assignees

浙江惠松制药有限公司

Dates

Publication Date: 20260505
Application Date: 20251215

Claims (10)

1. A method for identifying the morphology of fungus of Chinese-medicinal materials includes such steps as Step 1, marking according to a preset suspected fungus area to determine a hypha curve analysis position; Step 2, obtaining local image information corresponding to the hypha curve analysis position; step 3, analyzing the local image information to determine a reference hypha curve graph; Step 4, determining fixed parameters of the medicinal materials based on the hypha curve analysis position, wherein the fixed parameters of the medicinal materials comprise fixed coordinates and fixed angles of the medicinal materials; Step 5, generating a small ball striking scheme based on the medicinal material fixed parameters, wherein the small ball striking scheme comprises striking points and striking speeds; step 6, performing a pellet impact operation according to a pellet impact scheme, and determining a current hypha curve graph according to a hypha curve analysis position after the pellet impact operation; Step 7, determining a hypha curve variable diagram based on the current hypha curve diagram and a reference hypha curve diagram; And 8, determining hypha deformation quantity characteristics based on the hypha curve variable diagram, and analyzing the hypha deformation quantity characteristics to output hypha positions.
2. The method for identifying morphology of Chinese herbal medicine fungi according to claim 1, wherein the specific method for generating the ball striking scheme comprises the following steps: step 50, obtaining a medicinal material surface image to determine the type of medicinal material; Step 51, searching a safety impact strength interval corresponding to the medicinal material type in a preset medicinal material feature library based on the medicinal material type; step 52, determining the ball striking speed according to a preset ball mass and safety striking strength interval; and 53, determining a small ball impact point based on the medicinal material fixed parameters, and determining a small ball impact scheme according to the small ball impact point and the small ball impact speed.
3. The method for identifying morphology of Chinese medicinal fungi according to claim 2, wherein the method for determining the impact point of the pellets based on the fixed parameters of the medicinal materials comprises the following steps: Step 530, analyzing the surface image of the medicinal material to identify raised hypha areas and recessed hypha areas; Step 531, obtaining the height of the raised surface when the raised mycelium region exists; Step 532, determining the ball impact point according to the height of the convex surface and a preset height compensation formula; 533, obtaining the diameter of a concave opening when a concave mycelium area exists; Step 534, determining a small ball impact point according to the fixed parameters of the medicinal materials when the diameter of the opening of the concave is larger than the preset small ball diameter; Step 535, acquiring coordinates of a mycelium dense region at the edge of the concave opening when the diameter of the concave opening is smaller than that of the small ball; step 536, determining the ball impact point according to the coordinates of the mycelium dense region at the edge of the concave opening when the coordinates of the mycelium dense region at the edge of the concave opening exist.
4. The method for identifying morphology of fungi of traditional Chinese medicine according to claim 3, further comprising: Step 5320, analyzing the surface image of the medicinal material to determine adjacent raised areas corresponding to the raised hypha areas; step 5321, acquiring the coordinates of the raised hypha region and the coordinates of the adjacent raised regions; Step 5322, determining a current horizontal distance based on the adjacent raised area coordinates and the raised hypha area coordinates; Step 5323, redefining the ball impact point based on the current horizontal distance when the current horizontal distance is smaller than the ball diameter; step 5324 step 532 is performed when the current horizontal distance is greater than the diameter of the pellet.
5. A method for morphological identification of fungi of chinese medicinal material according to claim 3, further comprising determining a pellet impact protocol in the absence of coordinates of the mycelium-dense region at the edge of the recess opening, the method comprising: step 537, obtaining the center coordinates of the concave opening; step 538, determining a corrected ball impact point based on the coordinates of the center of the concave opening; Step 539, determining the maximum safe impact strength according to the safe impact strength interval, and using the maximum safe impact strength as a corrected small ball impact speed; Step 540, forming a ball striking scheme based on the modified ball striking point and the modified ball striking speed.
6. The method of claim 1, wherein analyzing the characteristics of the mycelium deformation amount to output the mycelium position comprises: Step 80, determining a predicted physical deformation curve trend based on a reference hypha curve graph, medicinal material fixed parameters and a small ball impact scheme, wherein the physical deformation curve trend comprises a predicted extension direction, a predicted bending angle range and a predicted curvature fluctuation threshold; step 81, determining the current mycelium curve trend based on the mycelium curve variable map; step 82, determining the position of the mycelium when the current mycelium curve trend is inconsistent with the physical deformation curve trend; And 83, outputting a preset collision trace signal when the current hypha curve trend is consistent with the physical deformation curve trend.
7. The method of claim 6, further comprising the step of not outputting a collision trace signal when the current mycelium curve trend is consistent with the physical deformation curve trend, the method comprising: Step 840, continuously obtaining the current hypha curve trend to determine the length change rate of the hypha curve; step 841, determining the position of the mycelium when the change rate of the length of the mycelium curve is not 0; Step 842, determining abnormal hyphae when the change rate of the length of the hypha curve is 0; step 843, determining a dyeing area when abnormal hyphae exist, and executing a preset dyeing operation according to the dyeing area to obtain a dyeing result; step 844, determining the position of hypha when the dyeing result is a continuous fluorescent network or a tubular fluorescent structure; step 845, outputting collision trace signal when the dyeing result is not continuous fluorescent network or tubular fluorescent structure.
8. The method for identifying morphology of fungus of Chinese medicinal material according to claim 7, wherein the method further comprises, before continuously obtaining the current mycelium curve trend: step 846, determining current hypha curve characteristics based on the local image information; step 847, analyzing the hypha curve characteristics to determine tubular structure characteristics and branch trace characteristics; Step 848, determining the position of the mycelium when the tubular structure feature and the branch trace feature exist simultaneously; Step 849, performing steps 840 through 845 when the tubular structural feature or branch trace feature is present; and 850, outputting a preset hypha absence signal when the tubular structure characteristic and the branch trace characteristic are absent.
9. The method for identifying the morphology of the fungus of the traditional Chinese medicinal material according to claim 8, further comprising the step of determining the standard rate of the drug effect after determining the position of the mycelium, wherein the method comprises the following steps: step 851, collecting microscopic images corresponding to hypha positions; 852, determining mycelium morphological feature parameters based on the microscopic image, wherein the mycelium morphological feature parameters comprise mycelium density, branching integrity and morphological integrity rate; step 853, inputting hypha morphological characteristic parameters into a preset prediction model to determine an effective component predicted value; Step 854, searching an effective component standard value corresponding to the medicinal material type in Chinese pharmacopoeia (2025 edition) based on the medicinal material type; and 855, determining the efficacy standard rate based on the predicted value of the effective component and the standard value of the effective component, and outputting the efficacy standard rate.
10. A system for identifying morphology of fungi of traditional Chinese medicinal materials, comprising: the acquisition module is used for acquiring the local image information and the type of the medicinal material; a memory for storing a program of a control method of the method for morphological identification of a fungus of a chinese medicinal material as defined in any one of claims 1 to 9; and the processor loads and executes the programs in the memory.

Description

Method and system for identifying morphology of fungi of traditional Chinese medicine Technical Field The invention relates to the field of quality detection of traditional Chinese medicinal materials, in particular to a method and a system for identifying morphology of fungi of traditional Chinese medicinal materials. Background The quality of the traditional Chinese medicine serving as an important carrier of the traditional Chinese medicine is directly related to clinical curative effect and medication safety. Fungal contamination is one of the core problems affecting the quality of traditional Chinese medicinal materials, namely, harmful fungi (such as aspergillus flavus and penicillium) can cause the mildew of the medicinal materials and generate toxins, and the morphological integrity of medicinal fungi (such as poria cocos and cordyceps sinensis) is directly related to the efficacy, so that accurate identification of the position and type of the fungi is a key of quality control. In the links of processing, transporting, storing and the like, the traditional Chinese medicinal materials inevitably generate surface physical damage due to mutual collision or external force action, and collision textures such as cracks, folds, pits and the like are formed, and the forms of the physical textures are highly similar to that of fungus threads in vision. Aiming at the related technology, an inspector relies on optical microscope inspection and experience to judge whether the surface of the medicinal material is covered with hypha or not, the overlap degree of collision texture and microscopic features of the hypha is high, and especially in the early growth stage of complex matrix of the Chinese medicinal material and fungi, the overlap degree of the complex matrix and fungi is far beyond expectations, and the physical injury and the biological hypha are difficult to distinguish, so that the misjudgment rate is high. Disclosure of Invention In order to effectively distinguish collision textures and true hyphae on the surfaces of traditional Chinese medicinal materials, the invention provides a method and a system for identifying the morphology of fungi of the traditional Chinese medicinal materials. In a first aspect, the invention provides a method for identifying morphology of fungi of traditional Chinese medicinal materials, which adopts the following technical scheme: A morphological identification method of fungus of Chinese medicinal materials comprises: Step 1, marking according to a preset suspected fungus area to determine a hypha curve analysis position; Step 2, obtaining local image information corresponding to the hypha curve analysis position; step 3, analyzing the local image information to determine a reference hypha curve graph; Step 4, determining fixed parameters of medicinal materials based on the hypha curve analysis position, wherein the fixed parameters of the medicinal materials comprise fixed coordinates of the medicinal materials and fixed angles of the medicinal materials, and the fixed parameters of the medicinal materials enable the hypha curve analysis position to face the ball impact direction; Step 5, generating a small ball striking scheme based on the medicinal material fixed parameters, wherein the small ball striking scheme comprises striking points and striking speeds; step 6, performing a pellet impact operation according to a pellet impact scheme, and determining a current hypha curve graph according to a hypha curve analysis position after the pellet impact operation; Step 7, determining a hypha curve variable diagram based on the current hypha curve diagram and a reference hypha curve diagram; And 8, determining hypha deformation quantity characteristics based on the hypha curve variable diagram, and analyzing the hypha deformation quantity characteristics to output hypha positions. By adopting the technical scheme, through suspected fungus area marking, local image acquisition and reference hypha curve analysis, and the combination of accurate regulation and control of fixed parameters of medicinal materials, the hypha curve analysis position is directionally impacted by the pellets. And generating a forming variable diagram by comparing the current hypha curve diagram with the reference hypha curve diagram after the impact, further extracting hypha deformation variable characteristics and outputting hypha positions. The method effectively distinguishes the morphological difference of the collision texture and true hypha on the surface of the traditional Chinese medicinal materials, solves the problem of misjudgment caused by overlapping of physical damage and biological hypha microscopic features in the traditional microscopic examination, and remarkably improves the accuracy and efficiency of fungus pollution identification. Optionally, the specific method for generating the small ball striking scheme comprises the following steps: step 50, obtaining a medicinal mate