CN-121978176-A - Pt/PEDOT composite modified carbon-based material needle electrode in-vivo dissolved oxygen electrochemical sensor and construction method and application thereof

Abstract

The invention provides a Pt/PEDOT composite modified carbon-based material needle electrode in-vivo dissolved oxygen electrochemical sensor and a construction method and application thereof, and belongs to the technical field of medical biology. The in-vivo dissolved oxygen electrochemical sensor utilizes a hexa-aqueous chloroplatinic acid solution and utilizes a time-current method to electrochemically deposit platinum nano particles to modify a carbon-based material microneedle electrode, and poly-3, 4-ethylenedioxythiophene is directly generated by electropolymerization on the surface of the Pt-modified microneedle electrode through an electrochemical constant-current method, so that uniform PEDOT film is formed on the microneedle electrode to wrap the modified platinum nano particles. The composite modified carbon-based material replaces noble metal to reduce cost and improve biocompatibility, optimizes low oxygen sensitivity by means of composite modification, and resists in-vivo protein adsorption and biomembrane coverage by anti-pollution surface modification, so that the defects are overcome, and the monitoring of in-vivo tissue oxygen partial pressure is realized.

Inventors

• WANG JIAFENG
• MO FENGFENG
• WEN JING
• DAI GE
• ZHAO ZHENZHEN
• BAO RUI
• LI DAI
• ZHOU YI
• WANG CHANGLI

Assignees

• 中国人民解放军海军军医大学

Dates

Publication Date

20260505

Application Date

20260205

Claims (9)

1. 1. The construction method of the Pt/PEDOT composite modified carbon-based material needle electrode in-vivo dissolved oxygen electrochemical sensor is characterized by utilizing platinum nano-particles and poly 3, 4-ethylenedioxythiophene (PEDOT).
2. 2. The method for constructing a Pt/PEDOT composite modified carbon-based material needle electrode in-vivo dissolved oxygen electrochemical sensor according to claim 1, wherein: The method comprises the steps of utilizing a hexa-aqueous chloroplatinic acid solution and electrochemically depositing platinum nano particles by means of a time-current method to modify a carbon-based material microneedle electrode, so as to obtain the Pt-modified microneedle electrode.
3. 3. The method for constructing a Pt/PEDOT composite modified carbon-based material needle electrode in-vivo dissolved oxygen electrochemical sensor according to claim 2, wherein: And directly electropolymerizing the surface of the Pt-modified microneedle electrode by an electrochemical constant current method to generate poly-3, 4-ethylenedioxythiophene, and uniformly wrapping the Pt-modified microneedle electrode by a PEDOT film on the microneedle electrode to obtain the Pt-modified microneedle electrode.
4. 4. The method for constructing an electrochemical sensor for dissolved oxygen in a needle electrode body of a Pt/PEDOT composite modified carbon-based material according to any one of claims 1 to 3, wherein, The method comprises the following steps: Step 1, preparing a PEDOT solution, namely adding 1.068 mu L of 3, 4-ethylenedioxythiophene into 1mL of 0.01mol/L polystyrene sulfonate aqueous solution, shaking and uniformly mixing, and placing the mixed solution at 4 ℃ for storage overnight to obtain the PEDOT solution; Step 2, depositing platinum nano particles, namely dissolving hexachloroplatinic acid hexahydrate into water to prepare a stock solution with the concentration of 0.5-2 wt%, then reducing and depositing the platinum nano particles on a carbon-based bare electrode by utilizing the stock solution and relying on a potentiostatic method to obtain a microneedle electrode modified with Pt, wherein the constant voltage used in the electrochemical deposition process is-1.5V, the scanning rate is 0.1V/s, the deposition time is 80-120s, and And step 3, performing PEDOT modification, namely forming a uniform PEDOT film on the microneedle electrode of the modified Pt by utilizing the PEDOT solution and performing electrodeposition through a constant current method to obtain the in-vivo dissolved oxygen electrochemical sensor.
5. 5. The method for constructing a Pt/PEDOT composite modified carbon-based material needle electrode in-vivo dissolved oxygen electrochemical sensor according to claim 4, wherein the method comprises the steps of: Wherein in step 1, the concentrations of the 3, 4-ethylenedioxythiophene and the aqueous solution of polystyrene sulfonate are the same and are any value of 0.01 to 0.02 mol/L.
6. 6. The method for constructing a Pt/PEDOT composite modified carbon-based material needle electrode in-vivo dissolved oxygen electrochemical sensor according to claim 4, wherein the method comprises the steps of: Wherein, in step 2, the concentration of the stock solution is 1 wt%.
7. 7. The method for constructing a Pt/PEDOT composite modified carbon-based material needle electrode in-vivo dissolved oxygen electrochemical sensor according to claim 4, wherein the method comprises the steps of: The step 3 specifically comprises the steps of inserting the microneedle electrode modified with Pt into the PEDOT solution, connecting a CHI660E electrochemical workstation, performing electrodeposition, setting a constant current of 0.1mA, setting a scanning rate of 0.002V/s, and setting the deposition time of 100s.
8. 8. The Pt/PEDOT composite modified carbon-based material needle electrode in-vivo dissolved oxygen electrochemical sensor is characterized in that the Pt/PEDOT composite modified carbon-based material needle electrode in-vivo dissolved oxygen electrochemical sensor is constructed by adopting the construction method of the Pt/PEDOT composite modified carbon-based material needle electrode in-vivo dissolved oxygen electrochemical sensor.
9. 9. Use of the Pt/PEDOT composite modified carbon-based material needle electrode in vivo dissolved oxygen electrochemical sensor according to claim 8 in vivo dissolved oxygen detection products.

Description

Pt/PEDOT composite modified carbon-based material needle electrode in-vivo dissolved oxygen electrochemical sensor and construction method and application thereof Technical Field The invention belongs to the technical field of medical biology, and particularly relates to a Pt/PEDOT composite modified carbon-based material needle electrode in-vivo dissolved oxygen electrochemical sensor, and a construction method and application thereof. Background The existing blood oxygen partial pressure detection method in hospitals is used for single-point analysis of automatic detection by a multi-purpose blood gas analyzer, and needs in-vitro blood sampling and on-machine detection, so that the blood oxygen level cannot be detected in real time. There is no electrochemical sensor that facilitates implantation within a blood vessel. Patent CN201810903984.2 discloses a three-electrode electrochemical dissolved oxygen sensor, which adopts a three-electrode structure, the working electrode is a gold electrode (core detection electrode), the auxiliary electrode is a double-spiral silver electrode (providing an electron transfer path), and the protection electrode is a platinum electrode (applying a protection voltage). The three electrodes are all made of traditional noble metal materials, and the cost is high. Although it is mentioned to be suitable for medical situations such as "blood dissolved oxygen detection", it does not involve invasive implantation of in vivo tissues (e.g. muscle, subcutaneous, vascular), it is more suitable for in vitro detection or short-term blood sampling detection, and it is not suitable for current clinical (e.g. intensive care unit) real-time detection. Therefore, the current dissolved oxygen electrochemical detection has the problems of high cost caused by precious metal dependence, defects of biocompatibility, incapability of meeting the in-vivo minimally invasive implantation requirement, insufficient detection sensitivity in a low-oxygen environment and the like. Disclosure of Invention The invention aims to solve the problems, and aims to provide a Pt/PEDOT composite modified carbon-based material needle electrode in-vivo dissolved oxygen electrochemical sensor, and a construction method and application thereof. The invention provides a construction method of a Pt/PEDOT composite modified carbon-based material needle electrode in-vivo dissolved oxygen electrochemical sensor, which has the characteristics that platinum nano-particles and poly 3, 4-ethylenedioxythiophene (PEDOT) are utilized for construction. The construction method of the Pt/PEDOT composite modified carbon-based material needle electrode in-vivo dissolved oxygen electrochemical sensor can also be characterized in that a platinum nanoparticle is electrochemically deposited and modified on a carbon-based material microneedle electrode by utilizing a hexa-aqueous chloroplatinic acid solution and by means of a time-current method, so that the modified Pt microneedle electrode is obtained. The construction method of the Pt/PEDOT composite modified carbon-based material needle electrode in-vivo dissolved oxygen electrochemical sensor provided by the invention can also be characterized in that the method is characterized in that poly-3, 4-ethylenedioxythiophene is directly generated by electropolymerization on the surface of a Pt-modified microneedle electrode by an electrochemical constant current method, and uniform PEDOT film wrapping modified platinum nano particles are formed on the microneedle electrode, so that the Pt-modified microneedle electrode is obtained. The construction method of the Pt/PEDOT composite modified carbon-based material needle electrode in-vivo dissolved oxygen electrochemical sensor provided by the invention can also have the characteristics that the method comprises the following steps of 1, preparing PEDOT solution, namely adding 1.068 mu L of 3, 4-ethylenedioxythiophene into 1mL of 0.01mol/L polystyrene sulfonate aqueous solution, vibrating and mixing uniformly, and storing the mixed solution at 4 ℃ overnight to obtain PEDOT solution; Step 2, depositing platinum nano particles, namely dissolving hexachloroplatinic acid hexahydrate into water to prepare a stock solution with the concentration of 0.5-2 wt%, then reducing and depositing the platinum nano particles on a carbon-based bare electrode by utilizing the stock solution and relying on a potentiostatic method to obtain a Pt-modified microneedle electrode, wherein the constant voltage used in the electrochemical deposition process is-1.5V, the scanning rate is 0.1V/s, the deposition time is 80-120s, and And 3, performing PEDOT modification, namely forming a uniform PEDOT film on the microneedle electrode modified with Pt by utilizing a PEDOT solution and performing electrodeposition through a constant current method to obtain the in-vivo dissolved oxygen electrochemical sensor. The construction method of the Pt/PEDOT composite modified carbon