CN-121978178-A - H-shaped electrolytic cell detection device and method integrating spiral separation function

CN121978178ACN 121978178 ACN121978178 ACN 121978178ACN-121978178-A

Abstract

The invention discloses an H-shaped electrolytic cell detection device and method integrating spiral separation function, which are used for rapid separation and detection of whole blood samples. The core design is that the spiral microfluidic separation module is in butt joint with a communication port at the top of a first chamber of the H-type electrolytic cell, so that the whole blood is separated by a spiral channel, and then the plasma directly enters a detection area, and blood cells are synchronously led out, thereby realizing the whole process integration of separation, incubation, cleaning and detection. The device comprises an H-shaped electrolytic cell main body (a porous membrane for fixing biological probes, a first/second chamber separated by the porous membrane) and a spiral microfluidic separation module (comprising a spiral channel, a sample inlet, an object outlet to be detected and a waste liquid outlet). The invention aims to solve the problems of complex pretreatment of blood samples, long time consumption and the like, realizes the separation and detection integration of the blood samples, combines the detection with a porous membrane, has the advantages of simple operation, high detection speed, high sensitivity, effective prevention of cross contamination and the like, and is suitable for instant detection and basic medical treatment.

Inventors

YANG ZHEN
LIU TIANTIAN
Xia Zengao
WANG XIJUN

Assignees

天津赛德医药研究院有限公司

Dates

Publication Date: 20260505
Application Date: 20260409

Claims (7)

1. The H-type electrolytic cell detection device integrating the spiral separation function is characterized by comprising an H-type electrolytic cell main body, a spiral microfluidic separation module and a Y-type or three-fork-shaped extension bifurcation outlet, wherein the H-type electrolytic cell main body is internally provided with a porous membrane for separating the H-type electrolytic cell main body into a first chamber and a second chamber, the spiral microfluidic separation module is in butt joint with a communication port at the top of the first chamber, whole blood is separated into blood cells and blood plasma through the synergistic effect of Dien resistance and inertial lift force in a spiral channel, a sample inlet, a Y-type or three-fork-shaped extension bifurcation outlet in fluid communication with the sample inlet is arranged on the spiral microfluidic separation module, the Y-type or three-fork-shaped extension bifurcation outlet comprises an object outlet and one or more waste liquid outlets, the object outlet is in fluid communication with the first chamber and is used for directly guiding separated blood plasma into the H-type electrolytic cell.
2. The H-type electrolytic cell detecting apparatus integrated with the spiral separating function according to claim 1, wherein the second chamber bottom of the H-type electrolytic cell is provided with a liquid discharge port.
3. The H-type electrolytic cell detection device integrated with the spiral separation function according to claim 1, wherein the porous membrane is made of polyethylene terephthalate (PET) or Polycarbonate (PC), and has a surface pore size of 0.01-10 μm and a pore density of 1 x 10 3 ~1×10 15 /cm 2 .
4. The H-type electrolytic cell detection device integrated with the spiral separation function according to claim 1, wherein a biological probe for capturing a specific disease marker is modified in a pore canal of the porous membrane, and the biological probe can be an antibody or an aptamer and is combined through a covalent bond.
5. The H-shaped electrolytic cell detection device integrating spiral separation function as claimed in claim 1, wherein the spiral microfluidic separation module comprises at least one spiral structure or a plurality of spiral structures communicated in parallel, an object outlet to be detected of the spiral microfluidic separation module is located on the outer side of a spiral channel, each waste liquid outlet is located on the inner side of the spiral channel, the cross section of the spiral channel is trapezoid or rectangular, the width of the spiral channel is 100-1000 μm, the height of the spiral channel is 20-400 μm, the number of spiral turns of the spiral channel is 2-20, the curvature radius of a central line of the first turn of the spiral channel is 1-10 mm, and the channel width ratio of the object outlet to be detected to the waste liquid outlet is 1:5-5:1.
6. A method for detecting an H-type electrolytic cell integrated with a spiral separation function according to any one of claims 1 to 5, comprising the steps of: S1, blood separation, namely adopting an H-shaped electrolytic cell detection device (a biological probe which is modified on a porous membrane and is used for capturing a specific disease marker) integrating spiral separation functions, injecting a blood sample into a sample inlet of a spiral microfluidic separation module, separating the sample into plasma and blood cells through a spiral channel, introducing the blood cells into a waste liquid outlet, introducing the blood cells into an electrolytic cell through a to-be-detected object outlet for incubation, opening a liquid outlet at the bottom of a second chamber after incubation is completed, and discharging waste liquid; s2, cleaning, namely closing a waste liquid outlet, injecting cleaning liquid into a sample inlet of the spiral microfluidic separation module, directly introducing the cleaning liquid into the electrolytic cell, and then discharging the cleaning liquid from a liquid discharge port; S3, electrochemical detection, namely closing a liquid outlet, injecting electrolyte into a sample inlet of the spiral microfluidic separation module, directly introducing the electrolyte into the H-shaped electrolytic cell, enabling an electrode to be in contact with the electrolyte through an electrode hole, and collecting electrochemical response signals of the porous membrane by utilizing an electrochemical detection device.
7. The detection method of the H-shaped electrolytic cell integrated with the spiral separation function according to claim 6, wherein the flow rate of the injected blood sample is 0.01 mL/min-3.0 mL/min, the flow rate of the injected cleaning liquid is 0.01 mL/min-3.0 mL/min, and the flow rate of the injected electrolyte is 0.01 mL/min-3.0 mL/min.

Description

H-shaped electrolytic cell detection device and method integrating spiral separation function Technical Field The invention belongs to the technical field of microfluidic and electrochemical biological detection, and particularly discloses an H-shaped electrolytic cell detection device and method integrating a spiral separation function. Background With the progress of science and the development of medical treatment, blood examination becomes an indispensable part of modern medical diagnosis, is an important means for disease diagnosis, and is currently a clinical examination blood sample, and a general clinical chemistry examination project uses serum or plasma in addition to a whole blood sample used in some projects such as blood lead, glycosylated hemoglobin, cyclosporine, tacrolimus and the like. Traditional blood separation methods (e.g., centrifugation) are complex to operate, take long time, rely on specialized equipment, and are difficult to meet the needs of point-of-care testing (POCT) and primary medical care. The microfluidic chip is used as a front-end technology platform and has wide application prospect in the fields of disease diagnosis, drug screening, environmental monitoring and the like. The spiral microfluidic separation chip based on the principle of inertial microfluidic can realize high-efficiency and label-free continuous separation of whole blood by optimizing the structural design of a micro-channel and utilizing the differences of inertial lift force (INERTIAL LIFT force) and Dean resistance (Dean drag) of blood cells and plasma components with different sizes in a spiral flow field. Provides technical support for early disease diagnosis. Electrochemical biosensors have the ability to detect rapidly, converting biological signals into electrical signals. The porous membrane has regular micro-channels and a controllable surface, can provide more stable active sites, and is often combined with an H-type electrolytic cell for detection. However, in the traditional detection flow, blood separation and electrochemical detection are step-by-step operation, the pretreated plasma sample is required to be manually transferred, and then steps such as incubation, cleaning, liquid adding and the like are carried out, so that the operation is complex, pollution risks are easy to introduce, and the detection efficiency is reduced. And when whole blood is directly injected, the risk of blocking the porous membrane exists in blood cells with larger diameters, so that the detection continuity and accuracy are affected. Although the microfluidic spiral separation chip can realize the high-efficiency separation of whole blood, the microfluidic spiral separation chip is not integrated with the detection function of the H-type electrolytic cell, so that the separation-detection is still a step-by-step operation, and the flow is not simplified. The spiral microfluidic structure and the H-shaped electrolytic cell are integrated to realize separation and detection integration, and the technology can reduce operation steps, shorten detection time, reduce pollution risk, improve detection efficiency, and effectively improve sensitivity by combining detection with a porous membrane. Disclosure of Invention The invention aims to provide an H-shaped electrolytic cell detection device and method integrating spiral separation function, and belongs to the technical field of electrochemical biological detection. The method aims at solving the problems of tedious pretreatment and long time consumption of the blood sample, realizes the separation and detection integration of the blood sample, and is suitable for the fields of instant detection and basic medical treatment. The invention provides an integrated spiral separation function integrated H-type electrolytic cell detection device which comprises an H-type electrolytic cell main body, a spiral microfluidic separation module and a sample inlet, wherein a porous membrane is arranged in the H-type electrolytic cell main body and is used for separating the H-type electrolytic cell main body into a first chamber and a second chamber, the spiral microfluidic separation module is in butt joint with a communication port at the top of the first chamber and is used for separating whole blood into blood cells and blood plasma through the synergistic effect of dean resistance and inertia lift force in a spiral channel, a sample inlet, a Y-shaped or three-fork-shaped extension bifurcation outlet which is in fluid communication with the sample inlet is arranged on the spiral microfluidic separation module, the Y-shaped or three-fork-shaped extension bifurcation outlet comprises an object outlet and one or more waste liquid outlets, the object outlet is in fluid communication with the first chamber and is used for directly guiding the separated blood plasma into the H-type electrolytic cell, and the waste liquid outlet is used for guiding the separated blood cells. Furth