Search

CN-121978182-A - Difunctional noble metal-nickel metal aerogel, wearable sweat bimodal enzyme-free glucose sensor and preparation method and application thereof

CN121978182ACN 121978182 ACN121978182 ACN 121978182ACN-121978182-A

Abstract

The invention discloses a difunctional noble metal-nickel metal aerogel, a wearable sweat bimodal enzyme-free glucose sensor, and a preparation method and application thereof, and relates to the technical field of metal aerogel. According to the method, firstly, the noble metal-nickel-based double-structure aerogel with high sensitivity and high selectivity is prepared to be used as a sensitive material, so that an electrochemical and colorimetric detection chip is prepared, and the detection chip and a microfluidic device are assembled into the wearable equipment. The double-mode enzyme-free detection of glucose in sweat by an electrochemical method and a colorimetric method is realized.

Inventors

  • WEN DAN
  • WAN XINHAO
  • MAO LIJUN
  • LIU WEIHUA

Assignees

  • 西北工业大学

Dates

Publication Date
20260505
Application Date
20260114

Claims (10)

  1. 1. A noble metal-nickel aerogel comprising PtNi aerogel or AuNiFe aerogel; The PtNi aerogel is prepared by mixing and reacting a mixed solution of H 2 PtCl 6 and NiCl 2 with a NaBH 4 solution and then freeze-drying, and the AuNiFe aerogel is prepared by mixing and reacting a mixed solution of HAuCl 4 、NiCl 2 and FeCl 3 with a NaBH 4 solution and then freeze-drying.
  2. 2. The precious metal-nickel metal aerogel of claim 1, wherein the molar ratio of the mixed solution of H 2 PtCl 6 and NiCl 2 to NaBH 4 solution is 1:1-40; the mass fraction of H 2 PtCl 6 in the mixed solution of H 2 PtCl 6 and NiCl 2 is 10 wt%; The molar ratio of H 2 PtCl 6 to NiCl 2 in the mixed solution of H 2 PtCl 6 and NiCl 2 is 1:0.1-6.
  3. 3. The precious metal-nickel metal aerogel according to claim 1, wherein the molar ratio of the mixed solution of HAuCl 4 、NiCl 2 and FeCl 3 to NaBH 4 solution is 1:1-40; The mass fraction of HAuCl 4 in the mixed solution of HAuCl 4 、NiCl 2 and FeCl 3 is 10 wt%; The molar ratio of HAuCl 4 、NiCl 2 、FeCl 3 in the mixed solution of HAuCl 4 、NiCl 2 and FeCl 3 is 1:0.1-6:0.01-2.
  4. 4. The precious metal-nickel metal aerogel according to claim 1, wherein the duration of the mixing reaction is between 6 and 24 h.
  5. 5. Use of the noble metal-nickel metal aerogel of any of claims 1-4 in a wearable sweat bimodal enzyme-free glucose sensor.
  6. 6. The wearable sweat bimodal enzyme-free glucose sensor based on the difunctional noble metal-nickel metal aerogel is characterized by comprising a PDMS (polydimethylsiloxane) circulation channel layer and a cover layer, wherein the PDMS circulation channel layer is arranged in a stacked manner, and the cover layer is covered on the PDMS circulation channel layer; The PDMS flow channel layer is provided with a plurality of liquid inlets, a first liquid storage cavity and a second liquid storage cavity; the first liquid storage cavity is communicated with the second liquid storage cavity through a first channel, each liquid inlet is communicated with the first liquid storage cavity through a second channel, the second liquid storage cavity is also provided with a liquid outlet, and the sealing layer is provided with a through hole corresponding to each liquid inlet; The electrochemical detection chip is arranged in the first liquid storage cavity, the colorimetric detection chip is arranged in the second liquid storage cavity, the electrochemical detection chip is made of PtNi aerogel, and the colorimetric detection chip is made of AuNiFe aerogel.
  7. 7. The dual-functional noble metal-nickel metal aerogel-based wearable sweat bimodal enzyme-free glucose sensor according to claim 6, wherein the electrochemical detection chip is prepared by preparing a dispersion liquid containing PtNi aerogel, nafion perfluorinated resin and ultrapure water, coating the dispersion liquid on the surface of a screen printing electrode working electrode, and drying under an infrared baking lamp, and obtaining the electrochemical detection chip after drying.
  8. 8. The dual function precious metal-nickel metal aerogel based wearable sweat bimodal enzyme-free glucose sensor of claim 6, wherein the colorimetric detection chip is prepared by preparing a dispersion containing AuNiFe aerogel and a dispersion containing 3,3', 5' -tetramethylbenzidine; and (3) modifying the dispersion liquid containing AuNiFe aerogel on the surface of a filter paper sheet, drying under an infrared baking lamp, adding a dispersion solution containing 3,3', 5' -tetramethyl benzidine after the drying is finished, and drying to obtain the colorimetric detection chip.
  9. 9. A method for preparing the wearable sweat bimodal enzyme-free glucose sensor based on a bifunctional noble metal-nickel metal aerogel according to any one of claims 6-8, comprising: Designing the structure of a PDMS flow channel layer, and preparing a die by a photoetching technology; preparing a PDMS (polydimethylsiloxane) circulation channel layer with a channel and a liquid storage cavity by using a reverse molding method, and processing a plurality of liquid inlets at specific positions of the PDMS circulation channel layer by using a puncher to prepare the PDMS circulation channel layer; And (3) performing oxygen plasma treatment on the top surface of the PDMS flow channel layer and the bottom surface of the sealing cover layer in a plasma processor, and then immediately performing bonding encapsulation according to the PDMS flow channel layer, the electrochemical detection chip, the colorimetric detection chip and the sealing cover layer to obtain the wearable sweat bimodal enzyme-free glucose sensor based on the difunctional noble metal-nickel metal aerogel.
  10. 10. Use of the wearable sweat bimodal enzyme-free glucose sensor based on a bifunctional noble metal-nickel metal aerogel according to any one of claims 6-8 in sweat bimodal glucose sensing detection equipment.

Description

Difunctional noble metal-nickel metal aerogel, wearable sweat bimodal enzyme-free glucose sensor and preparation method and application thereof Technical Field The invention relates to the technical field of metal aerogel, in particular to a difunctional noble metal-nickel metal aerogel, a wearable sweat bimodal enzyme-free glucose sensor, a preparation method and application thereof. Background Glucose is an important substance for energy metabolism of human cells, and has various physiological functions such as providing energy, maintaining functions of a nervous system, enhancing immunity and the like. Diabetes is a chronic metabolic disease characterized by hyperglycemia, often causing chronic damage and dysfunction to tissues such as kidneys, heart, blood vessels, and nerves. The blood sugar real-time monitoring system can provide continuous blood sugar data, so that a user can know the change trend and fluctuation range of blood sugar in real time, especially for diabetics, the blood sugar level can be monitored in time, and diet, medicine or insulin treatment scheme can be adjusted according to the requirement. The existing glucose sensor is mainly a continuous blood glucose monitoring sensor which is a blood glucose meter or a semi-implanted type and is used for effectively detecting the concentration of glucose. However, the existing detection equipment has the problems that 1) pain is possibly caused in the detection process, potential infection risks exist, continuous monitoring is difficult to realize in daily life, 2) most commercial glucose sensors depend on biological enzymes as catalysts, the use condition and stability of the sensors are limited, and 3) most glucose sensors are subjected to electrochemical or colorimetric and other single-mode sensing modes, and numerical value generation and other risks exist. The wearable sweat glucose sensor collects sweat in a noninvasive manner by designing a microfluidic device, and converts the glucose concentration in the sweat into a signal which can be directly identified, and indirectly reflects the glucose level of a human body, so that the wearable sweat glucose sensor has a wide application prospect. The inorganic catalytic material can replace biological enzyme as a glucose sensitive material to ensure that the sensor maintains high sensitivity and the stability of the sensor is obviously improved. The electrochemical-colorimetric bimodal detection mode can meet the real-time detection of sweat glucose level in a complex environment, reduce errors and improve detection accuracy. Disclosure of Invention Aiming at the defects in the background technology, the invention mainly solves the technical problems of the existing commercial glucose detection method in the aspects of wound sampling and detection accuracy and reliability. The invention provides a difunctional noble metal-nickel metal aerogel, a wearable sweat bimodal enzyme-free glucose sensor, a preparation method and application thereof. The method comprises the steps of preparing and modifying sensitive materials, designing and processing a PDMS flow channel layer, and assembling a sensor. The sensor can obtain accurate monitoring of sweat glucose concentration, and can show excellent long-term stability and anti-interference capability in practical application. The technology provides an effective and reliable solution for accurate health monitoring of human bodies. A first object of the present invention is to provide a noble metal-nickel aerogel, including PtNi aerogel or AuNiFe aerogel; The PtNi aerogel is prepared by mixing and reacting a mixed solution of H 2PtCl6 and NiCl 2 with a NaBH 4 solution and then freeze-drying, and the AuNiFe aerogel is prepared by mixing and reacting a mixed solution of HAuCl 4、NiCl2 and FeCl 3 with a NaBH 4 solution and then freeze-drying. Preferably, the molar ratio of the mixed solution of H 2PtCl6 and NiCl 2 to the NaBH 4 solution is 1:1-40; the mass fraction of H 2PtCl6 in the mixed solution of H 2PtCl6 and NiCl 2 is 10 wt%; The molar ratio of H 2PtCl6 to NiCl 2 in the mixed solution of H 2PtCl6 and NiCl 2 is 1:0.1-6. Preferably, the molar ratio of the mixed solution of HAuCl 4、NiCl2 and FeCl 3 to the NaBH 4 solution is 1:1-40; The mass fraction of HAuCl 4 in the mixed solution of HAuCl 4、NiCl2 and FeCl 3 is 10 wt%; The molar ratio of HAuCl 4、NiCl2、FeCl3 in the mixed solution of HAuCl 4、NiCl2 and FeCl 3 is 1:0.1-6:0.01-2. Preferably, the duration of the mixing reaction is from 6 to 24 h. The second object of the invention is to provide an application of noble metal-nickel metal aerogel in a wearable sweat bimodal enzyme-free glucose sensor. The invention provides a wearable sweat bimodal enzyme-free glucose sensor based on a difunctional noble metal-nickel metal aerogel, which comprises a PDMS (polydimethylsiloxane) flow channel layer and a cover layer, wherein the PDMS flow channel layer is arranged in a stacked manner, and the cover layer is cov