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CN-121978231-A - Method for detecting content of main active ingredients in ginger and licorice decoction

CN121978231ACN 121978231 ACN121978231 ACN 121978231ACN-121978231-A

Abstract

The invention belongs to the technical field of detection of components of traditional Chinese medicine preparations, and particularly relates to a method for detecting the content of main active components in ginger and licorice decoction. The content detection method of the invention adopts HPLC chromatography to detect flavonoid, triterpenoid saponins and phenols in the raw Jiang Gancao decoction. According to the invention, through specific sample pretreatment and chromatographic conditions, the main active ingredients of the key medicines of ginger and liquorice in the ginger and liquorice decoction, namely the liquiritin, the apioside, the isoliquiritigenin, the glycyrrhizin, the monoammonium glycyrrhizinate and the 6-gingerol, can be quantitatively detected at the same time, so that the clinical efficacy of the ginger and liquorice decoction preparation is ensured, and a more comprehensive method is provided for quality control of ginger and liquorice decoction.

Inventors

  • HUANG YU
  • LIN XIAOYAN
  • DENG YUE
  • JIANG YANJIAO
  • CHEN LONGHU

Assignees

  • 四川新绿色药业科技发展有限公司

Dates

Publication Date
20260505
Application Date
20251219

Claims (10)

  1. 1. A method for detecting the content of main active ingredients in ginger and licorice soup is characterized by comprising the following operation steps: 1) Preparing reference solution by adding methanol solution into reference solution of flavonoid compound, triterpenoid saponin compound and phenolic compound; 2) Preparing test solution by extracting rhizoma Zingiberis recens Glycyrrhrizae radix decoction with methanol solution to obtain test solution; 3) The detection comprises adopting HPLC chromatography, wherein chromatographic conditions are as follows, a chromatographic column takes octadecylsilane chemically bonded silica as a filler, a mobile phase takes acetonitrile as a mobile phase A, 0.1% phosphoric acid solution as a mobile phase B, and gradient elution is carried out for 0-60 min,20% -45% A,80% -55% B.
  2. 2. The method for detecting the content of the substances according to claim 1, wherein each 1ml of the reference substance solution in the step 1) contains 50-150. Mu.g of each reference substance.
  3. 3. The method for detecting the content according to claim 1 or 2, wherein the flavonoid compound in the reference substance is liquiritin, apioside, isoliquiritigenin and/or glycyrrhizin, the triterpenoid saponin compound is monoammonium glycyrrhizinate, and the phenolic compound is 6-gingerol.
  4. 4. The content detection method according to claim 1, wherein the mass-volume ratio of the ginger and licorice decoction preparation to the methanol solution in the step 2) is 0.2 g:5-25 ml, and the concentration of the methanol solution is 70%.
  5. 5. The method according to claim 1 or 4, wherein the ginger and licorice decoction is prepared by adding pharmaceutically acceptable auxiliary materials into ginger, licorice, ginseng and jujube.
  6. 6. The method of claim 5, wherein the preparation comprises rhizoma Zingiberis recens radix Glycyrrhizae Shang Shangji, rhizoma Zingiberis recens radix Glycyrrhizae granule, rhizoma Zingiberis recens radix Glycyrrhizae Shang Fenji, rhizoma Zingiberis recens radix Glycyrrhizae Shang Wanji, and rhizoma Zingiberis recens radix Glycyrrhizae decoction.
  7. 7. The method for detecting the content of ZORBAX SB-Aq according to claim 1, wherein the chromatography column in the step 3) is ZORBAX SB-Aq 4.6X105 mm 3.5. Mu.m.
  8. 8. The content detection method according to claim 1, wherein the column temperature of the chromatographic condition in the step 3) is 25-35 ℃, the flow rate is 0.8-1.2 mL/min, the sample injection amount is 1-15 mu L, and the wavelength is 230-270 nm.
  9. 9. The method for detecting the content of the organic light emitting diode according to claim 1, wherein the column temperature is 25 ℃, the flow rate is 1.0mL/min, the sample injection amount is 5 mu L, and the wavelength is 230nm.
  10. 10. The method for detecting the content according to any one of claims 1 to 9, wherein the content is calculated by a standard curve method.

Description

Method for detecting content of main active ingredients in ginger and licorice decoction Technical Field The invention belongs to the technical field of detection of components of traditional Chinese medicine preparations, and particularly relates to a method for detecting the content of main active components in ginger and licorice decoction. Background The ginger and licorice decoction is first found in Qianjin Fang of Tang Dynasty and Sun, and the prescription is composed of ginger, licorice, ginseng and jujube, and is a classical prescription of traditional Chinese medicine. The whole formula takes ginger as a monarch for dispelling cold and resolving drink, ginseng for reinforcing primordial qi, licorice for nourishing yin and tonifying yang in sweet and slow conservation, jujube for harmonizing ying and wei, and peganum for generating gold. Is mainly used for treating symptoms such as consumptive lung disease, cough, saliva, etc. The Chinese medicinal composition has the core effects of warming and invigorating spleen and stomach, tonifying qi and promoting the production of body fluid, moistening lung and resolving phlegm, and is also suitable for the symptoms of deficiency-cold of spleen and stomach, deficiency of lung qi and the like. Ginger and licorice are key herbs in the ginger and licorice decoction, and ginger is pungent and warm, licorice can alleviate the pungent nature of ginger, so that the efficacy is milder and lasting, and the two herbs have unique positions in the raw Jiang Gancao decoction. 6-gingerol is a representative functional component in the monarch drug ginger, and glycyrrhizin, apinoside, isoliquiritigenin, glycyrrhizin and monoammonium glycyrrhizinate are important components in licorice, especially the content of the glycyrrhizin and monoammonium glycyrrhizinate in the licorice is relatively high, and the components are closely related to the exertion of the efficacy of ginger licorice soup, however, no quantitative research on active components in the ginger licorice soup exists at present, and no report on quantitative methods of the main active components exists. Therefore, it is necessary to provide a method for quantifying the main active ingredients in the ginger decoction, so as to control the quality of the ginger decoction, and ensure the efficacy of the ginger decoction. Disclosure of Invention In order to solve the problems, the invention establishes a method for detecting the content of main active ingredients in ginger and licorice decoction, which comprises the following operation steps: 1) Preparing reference solution by adding methanol solution into reference solution of flavonoid compound, triterpenoid saponin compound and phenolic compound; 2) Preparing test solution by extracting rhizoma Zingiberis recens Glycyrrhrizae radix decoction with methanol solution to obtain test solution; 3) The detection comprises adopting HPLC chromatography, wherein chromatographic conditions are as follows, a chromatographic column takes octadecylsilane chemically bonded silica as a filler, a mobile phase takes acetonitrile as a mobile phase A, 0.1% phosphoric acid solution as a mobile phase B, and gradient elution is carried out for 0-60 min,20% -45% A,80% -55% B. Further, each 1ml of the reference substance solution in the step 1) contains 50-150 mug of each reference substance. Further, the flavonoid compound in the reference substance is glycyrrhizin, apioside isoliquiritigenin, isoliquiritigenin and/or glycyrrhizin, the triterpenoid saponin compound is monoammonium glycyrrhizinate, and the phenolic compound is 6-gingerol. Further, in the step 2), the mass-volume ratio of the ginger and licorice decoction preparation to the methanol solution is 0.2 g:5-25 ml. Still further, the concentration of the methanol solution was 70%. Furthermore, the ginger and licorice decoction is prepared by taking ginger, licorice, ginseng and jujube as raw material medicines and adding pharmaceutically acceptable auxiliary materials; The mass ratio of the ginger to the liquorice to the ginseng to the jujube is 65-70:52-58:40-45:32-38. Further, the mass ratio of ginger, liquorice, ginseng and jujube is 69:55.2:41.4:36. Further, the ginger decoction preparation comprises ginger decoction Shang Shangji, ginger decoction granules, ginger decoction Shang Fenji, ginger decoction Shang Wanji and ginger decoction substance references; Further, the column of step 3) was ZORBAX SB-Aq 4.6X105 mm 3.5. Mu.m. Further, the column temperature of the chromatographic condition in the step 3) is 25-35 ℃, the flow rate is 0.8-1.2 mL/min, the sample injection amount is 1-15 mu L, and the wavelength is 230-270 nm. Further, the column temperature was 25 ℃, the flow rate was 1.0mL/min, the sample injection amount was 5. Mu.L, and the wavelength was 230nm. Further, it calculates the content by standard curve method. The ginger and licorice decoction is named as a Chinese medicine prescription, and is prepared from