CN-121978241-A - Automatic gel permeation chromatography system

CN121978241ACN 121978241 ACN121978241 ACN 121978241ACN-121978241-A

Abstract

The invention relates to the technical field of high molecular material analysis and test, in particular to an automatic gel permeation chromatography system. In order to solve the technical problem of improving the degree of automation of analysis and detection. The technical scheme includes that the automatic gel permeation chromatography system comprises an automatic sample feeding unit, a chromatography separation unit, an on-line detection unit, a central control unit, a constant-current infusion pump and a chromatography column device, wherein the automatic sample feeding unit is used for receiving a sample and automatically feeding the sample, the chromatography separation unit comprises the constant-current infusion pump and the chromatography column device, the constant-current infusion pump is used for receiving the sample feeding of the automatic sample feeding unit and performing chromatography separation, the constant-current infusion pump is used for conveying the sample to the chromatography column device, the on-line detection unit comprises at least one differential detector, the differential detector is used for collecting electric signals of components separated by the chromatography separation unit, the central control unit controls the automatic sample feeding unit to inject the sample into the chromatography separation unit, controls the constant-current infusion pump to carry out sample conveying and controls the chromatography column device to carry out chromatography separation, and controls the on-line detection unit to collect the electric signals of the components separated by the chromatography separation unit.

Inventors

LI HAORAN
SUN JUNREN
YU CHUANZHOU
WANG CHENGCHENG
WANG FENGLIN
LI YABO
LI TONG

Assignees

苏州依利特科技有限公司

Dates

Publication Date: 20260505
Application Date: 20260212

Claims (10)

1. An automatic gel permeation chromatography system is characterized by comprising an automatic sample injection unit, a chromatographic separation unit, an online detection unit, a central control unit and a data processing unit; The automatic sample injection unit is used for receiving the prepared sample and performing automatic sample injection; The chromatographic separation unit comprises a constant-flow infusion pump and a chromatographic column device, wherein the chromatographic column device receives sample injection of the automatic sample injection unit and performs chromatographic separation; The on-line detection unit comprises at least one differential detector, wherein the differential detector is used for collecting electric signals of components separated by the chromatographic separation unit; The central control unit is in communication connection with the automatic sample injection unit, the chromatographic separation unit and the online detection unit, and is configured to control the automatic sample injection unit to inject a sample into the chromatographic separation unit, control the constant-current infusion pump to carry out sample conveying and control the chromatographic column device to carry out chromatographic separation, and control the online detection unit to collect electric signals of components separated by the chromatographic separation unit.
2. An automated gel permeation chromatography system according to claim 1, wherein, The chromatographic separation unit comprises a chromatographic column incubator and at least one chromatographic column arranged in the chromatographic column incubator, and further comprises a degasser module which is connected in series with the constant-flow infusion pump.
3. The automated gel permeation chromatography system of claim 2, wherein the automated gel permeation chromatography system comprises a sample bottle and a waste liquid bottle, and the sample bottle is sequentially connected with the degassing module, the constant flow infusion pump, the automatic sample injection unit, the chromatographic column, the differential detector and the waste liquid bottle through pipelines.
4. An automated gel permeation chromatography system according to claim 3, wherein the autosampler unit comprises a sample tray for holding a sample, a reagent bottle, a sample needle, and an XYZ axis motion assembly comprising an X axis slide assembly, a Y axis slide assembly, and a Z axis slide assembly; The X-axis sliding assembly is in transmission connection with the Z-axis sliding assembly, and the Z-axis sliding assembly is connected with a sample injection needle which is movably arranged above the sample tray; The X-axis sliding component is in transmission connection with the sample tray, and the X-axis sliding component can drive the sample tray to move along the X-axis direction.
5. The automated gel permeation chromatography system of claim 4, wherein the X-axis slide assembly comprises a slide rail slide block assembly mounted in an X-axis direction above the sample tray, a first lead screw assembly drivingly connected to the slide rail slide block assembly and extending in the X-axis direction, a first belt and gear drive assembly drivingly connected to the first lead screw assembly, and an X-axis motor drivingly connected to the first belt and gear drive assembly and connected to the Z-axis slide assembly.
6. The automated gel permeation chromatography system according to claim 4, wherein the Y-axis sliding assembly comprises a slide plate support mounted at the bottom of the sample tray, a sliding table connected to the slide plate support along the Y-axis direction, a second screw assembly drivingly connected to the slide plate support along the Y-axis direction, a second belt-gear assembly drivingly connected to the second screw assembly, and a Y-axis motor drivingly connected to the second belt-gear assembly.
7. The automated gel permeation chromatography system of claim 5, wherein the Z-axis sliding assembly comprises a fixed plate coupled to the first screw assembly, a third belt-gear assembly disposed above the fixed plate, a Z-axis motor drivingly coupled to the third belt-gear assembly, and a third screw assembly drivingly coupled to the third belt-gear assembly, wherein the third screw assembly is coupled to the sample injection needle along the Z-axis.
8. The automated gel permeation chromatography system of claim 4, wherein the autosampler unit further comprises a dosing ring, a six-way valve, and a two-way assembly; The constant flow infusion pump is connected with an inlet of the two-way assembly through a pipeline, the inlet of the two-way assembly is connected with a port 4 of the six-way valve through a pipeline, the port of the six-way valve is connected with an outlet of the two-way assembly, the outlet of the two-way assembly is connected with one end of the chromatographic column through a pipeline, the other end of the chromatographic column is connected with the differential detector through a pipeline, and the differential detector is connected with the waste liquid bottle through a pipeline.
9. The automated gel permeation chromatography system of claim 8, wherein the two ends of the dosing ring are connected to port 3 and port 6 of the six-way valve respectively via tubing, the sample injection needle is connected to port 2 of the six-way valve via tubing, the syringe is connected to port 1 of the six-way valve via tubing, and the syringe is connected to the injection motor.
10. The automated gel permeation chromatography system of claim 4, further comprising a sample tray transfer area disposed on a side of the sample tray of the autosampler unit, and a robotic arm device, wherein the differential detector is disposed between the sample tray transfer area and the robotic arm device.

Description

Automatic gel permeation chromatography system Technical Field The invention relates to the technical field of high molecular material analysis and test, in particular to an automatic gel permeation chromatography system. Background Gel permeation chromatography (Gel Permeation Chromatography, abbreviated as GPC) works principally by separating and identifying the gel by a chromatographic system using its chemical inertness. The chromatographic system comprises a pump, a sample injector, a chromatographic column, a detector, a data analysis system and the like. However, with the increase of test requirements, the original GPC system has various problems such as complicated manual operation, high manual dependency on steps such as sample injection, system balance, data post-processing and the like, low automation degree of analysis and detection, manual monitoring on system stability, low data analysis efficiency and the like. Disclosure of Invention In order to solve the technical problem of improving the degree of automation of analysis and detection, the invention provides the following technical scheme: An automatic gel permeation chromatography system comprises an automatic sample injection unit, a chromatographic separation unit, an online detection unit, a central control unit and a data processing unit; the automatic sample injection unit is used for receiving a sample and performing automatic sample injection; The chromatographic separation unit comprises a constant-flow infusion pump and a chromatographic column device, wherein the chromatographic column device receives sample injection of the automatic sample injection unit and performs chromatographic separation; The on-line detection unit comprises at least one differential detector, wherein the differential detector is used for collecting electric signals of components separated by the chromatographic separation unit; The central control unit is in communication connection with the automatic sample injection unit, the chromatographic separation unit and the online detection unit, and is configured to control the automatic sample injection unit to inject a sample into the chromatographic separation unit, control the constant-current infusion pump to carry out sample conveying and control the chromatographic column device to carry out chromatographic separation, and control the online detection unit to collect electric signals of components separated by the chromatographic separation unit. Further, the chromatographic separation unit comprises a chromatographic column incubator and at least one chromatographic column arranged in the chromatographic column incubator, and further comprises a degasser module which is connected in series with the constant-flow infusion pump. Further, the automatic gel permeation chromatography system comprises a sample bottle and a waste liquid bottle, wherein the sample bottle is sequentially connected with the degassing module, the constant-flow infusion pump, the automatic sample injection unit, the chromatographic column, the differential detector and the waste liquid bottle through pipelines. Further, the automatic sample feeding unit comprises a sample tray for storing samples, a reagent bottle, a sample feeding needle and an XYZ axis movement assembly, wherein the XYZ axis movement assembly comprises an X axis sliding assembly, a Y axis sliding assembly and a Z axis sliding assembly; The X-axis sliding assembly is in transmission connection with the Z-axis sliding assembly, and the Z-axis sliding assembly is connected with a sample injection needle which is movably arranged above the sample tray; The X-axis sliding component is in transmission connection with the sample tray, and the X-axis sliding component can drive the sample tray to move along the X-axis direction. Further, the X-axis sliding assembly comprises a sliding rail sliding block assembly arranged above the sample tray in the X-axis direction, a first screw rod assembly in transmission connection with the sliding rail sliding block assembly, a first belt gear transmission assembly in transmission connection with the first screw rod assembly, and an X-axis motor in transmission connection with the first belt gear transmission assembly, wherein the first screw rod assembly is connected with the Z-axis sliding assembly. Further, the Y-axis sliding assembly comprises a sliding plate support arranged at the bottom of the sample tray, a sliding table connected with the sliding plate support along the Y-axis direction, a second screw rod assembly in transmission connection with the sliding plate support along the Y-axis direction, a second belt gear transmission assembly in transmission connection with the second screw rod assembly, and a Y-axis motor in transmission connection with the second belt gear transmission assembly. Further, the Z-axis sliding assembly comprises a fixed plate connected with the first screw rod assembly, a third belt gear trans