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CN-121978242-A - Specific method for identifying red seed coat peanuts based on high antioxidant active substances

CN121978242ACN 121978242 ACN121978242 ACN 121978242ACN-121978242-A

Abstract

The invention discloses a specific method for identifying red seed coat peanuts based on high antioxidant active substances, which is used for identifying or identifying peanut materials for removing the skin or other industrial and agricultural peanut materials, constructing a specific chromatographic analysis path based on the newly discovered specific chemical components in the red seed coat peanuts, and identifying the red seed coat peanut raw materials based on the specific characteristic chromatographic peaks of the red seed coat peanut materials. For common materials such as common peanut protein powder, dehulled peanut seeds and the like, the invention can directly identify whether the agricultural and industrial peanut raw materials are red seed coat peanut sources or not without depending on the integrity of seed coats, and provides an important technical basis for quality control and variety identification of the peanut raw materials.

Inventors

  • ZHAO XING
  • LIU YIQI
  • LIU BOYAN
  • PU LIN
  • WANG JIN
  • LIU SIJIE

Assignees

  • 石家庄学院

Dates

Publication Date
20260505
Application Date
20240625

Claims (10)

  1. 1. The novel application of quercetin-3-O-rutinoside-7-O-xylose/arabinoside is characterized by being used for the specific identification of red seed-skin peanuts.
  2. 2. The new application of quercetin-3-O-rutinoside-7-O-xylose/arabinoside is characterized in that the quercetin-3-O-rutinoside-xylose/arabinoside is used as a high antioxidant active substance in food, medicines, cosmetics and chemical industry.
  3. 3. The method for identifying the red seed coat peanut raw material independently of the integrity of the seed coat is used for identifying or identifying the peanut materials for removing the skin or other industrial and agricultural peanut materials, and is characterized in that a specific chromatographic analysis path is constructed based on the newly discovered special chemical components in the red seed coat peanut, and the identification and identification of the red seed coat peanut raw material are carried out based on the special characteristic chromatographic peaks of the red seed coat peanut materials, wherein the special chemical components are single components or multiple components with extremely high antioxidant activity.
  4. 4. The method for identifying red seed coat peanut raw material independent of seed coat integrity of claim 3, wherein said specific chemical components include, but are not limited to, quercetin-3-O-rutinoside-7-O-xylose/arabinoside.
  5. 5. The method for identifying red seed coat peanut raw material independent of seed coat integrity according to claim 4, wherein the specific chromatographic path comprises the following implementation steps: A. Naturally air-drying peanut materials to be tested until the water content is below 5%, selecting mature full and unbroken peanut kernels, grinding the peanut kernels by a grinder, and sieving the ground peanut kernels with a 20-mesh sieve to obtain sample powder to be tested; B. Preparing an extracting solution, namely, taking sample powder to be detected, namely, 0.1 g, precisely weighing, placing the sample powder into a2 mL centrifuge tube, adding n-hexane for degreasing treatment twice, precisely adding 50% methanol aqueous solution (V/V) according to the proportion of 1g to 15 mL (m/V), uniformly mixing, soaking 30min, performing ultrasonic auxiliary extraction at room temperature, 250 W,40 kHz,30 min, shaking every 10 min, centrifuging at room temperature, precisely measuring supernatant, and obtaining a primary extracting solution; C. Taking 1, 1-diphenyl-2-trinitrophenylhydrazine DPPH, precisely adding methanol according to the proportion of 12.7 mL of methanol added into each 1 mg DPPH, preparing 200 mu mol/L DPPH methanol solution, dissolving 1 min,250 W,40 kHz in an ultrasonic-assisted manner, fully and uniformly mixing, and filtering by using a 0.2 mu m microporous filter membrane to obtain a characteristic extracting solution; D. Mixing the primary extract with 2 times of methanol, standing for 60 min to obtain primary extract, mixing the primary extract with 200 μmol/L characteristic extract, and performing light-shielding reaction for 60 min to obtain secondary extract; E. Chromatographic analysis, analysis of chemical components in the extractive solution by using ultra-high performance liquid chromatography system under conditions of Agilent ZORBAX SB-C18, 2.1X100 mm,1.8 μm, 35 deg.C, 0.2% (v/v) formic acid aqueous solution as mobile phase A, 0.35 mL/min as mobile phase B, 2.0 μl sample injection, 354 detection wavelength nm, and elution ：0～0.5 min,10% B;0.5～1 min,10%～15% B;1.0～2.5 min,11% B;2.5～9.5 min,11%～25% B;9.5～11.0 min,25% B;11.0～13.5 min,25%～40% B;13.5～15.0 min,40%～95% B;15.0～22.0 min,10% B; F. The method comprises the steps of performing dual characteristic analysis, and identifying red peanut seeds based on the dual characteristic analysis on a chromatographic spectrum, wherein the dual analysis comprises a first step of determining whether a first-stage extracting solution has characteristic chromatographic peaks in a specific node area or a specific node site on the chromatographic spectrum, a second step of determining whether the first-stage extracting solution and the second-stage extracting solution have characteristic chromatographic peak defects in the specific node area or the specific node site on the chromatographic spectrum, and meanwhile, the materials to be detected which pass through the first step and the second step are determined to be the red peanut seeds.
  6. 6. The method for identifying red seed coat peanut raw materials independent of seed coat integrity according to claim 5, wherein the specific node interval is a node interval of 6-7.5 min on a chromatographic chart.
  7. 7. The method for identifying red seed coat peanut raw materials independent of seed coat integrity according to claim 5, wherein the specific node interval is a node interval of 6.5-7 min on a chromatographic chart.
  8. 8. The method for identifying red seed coat peanut raw material independent of seed coat integrity as in claim 5, wherein the specific node interval is a smaller interval around 6.7min on a chromatographic chart.
  9. 9. A method for identifying red peanut materials from common peanut groups, which is characterized in that peanut protein powder, dehulled peanut powder and dehulled peanut kernels are identified by adopting the method of any one of claims 3-8 to identify and distinguish red peanut, wherein the common peanut groups comprise, but are not limited to Ji Hua-5211, four-seed red, red-fora red-bean peanuts, four-seed red-river flowers, one-nest monkey in North China, 16-flower flowers, 13-flower flowers, 21-flower flowers, 19-flower flowers, 18-flower flowers, 1-king black pearl, 9-flower flowers, 3-flower black flowers, small-girls, y21326 peanuts, y21202 peanuts, y21201 peanuts, 21-61169 peanuts, 21-zw072 peanuts and 1-flower sweet.
  10. 10. The new application of the red seed coat peanuts is characterized in that the red seed coat peanuts are used as a high-activity antioxidant substance extraction source, or are used as food raw materials with higher antioxidant activity relative to non-red peanut peanuts, or the artificial addition of antioxidants is reduced relative to the non-red peanut peanuts, and the shelf life of peanut products is prolonged.

Description

Specific method for identifying red seed coat peanuts based on high antioxidant active substances The application relates to a specific identification method and new application of red seed coat peanut raw materials, which are applied separately from patent application with the application number of 202410827655.X and the application date of 2024-06-25. Technical Field The invention relates to the technical field of chromatographic analysis, in particular to a specific identification method of red seed coat peanut raw materials and subsequent development technology and application development thereof. Background Peanuts are important oil and commercial crops and are also an important source of various food-borne functional ingredients. Peanut skin is rich in functional components and has medicinal value. However, during processing of peanut oil or peanut food, peanut skin is typically removed, making it difficult for the chemical components contained therein to function in peanut related products. In contrast, the functional components contained in the red-removed peanut kernels coexist with the nutritional components such as crude fat, crude protein and the like in the peanut kernels, so that the red-removed peanut kernels are more convenient for human to ingest, and have more positive effects on meeting the high-quality nutritional requirements of human and the healthy development of the peanut industry. However, there is a significant difference in the functional components of peeled peanut kernels from peanut varieties of different seed coat colors. At present, the seed coats of the cultivated peanuts are pink, red, black purple, white and the like. The present invention has been made in view of the above-described problems, and has as its object the finding that there are a series of specific chemical components in the peanut kernel of the red seed coat, which have extremely high antioxidant activity. Further development of a method for identifying the source of materials for removing the coating of peanut kernels or powder based on these findings is of great practical importance. Disclosure of Invention The invention aims to solve the technical problem of providing a specific method for identifying red seed coat peanuts based on high-antioxidant active substances based on the blank of the prior art. In order to solve the technical problems, the technical scheme adopted by the invention is as follows. The invention comprises the novel application of quercetin-3-O-rutinoside-7-O-xylose/arabinoside, and is used for the specific identification of red seed coat peanuts. The invention also comprises a new application of quercetin-3-O-rutinoside-7-O-xylose/arabinoside as a high antioxidant active substance for food, medicines, cosmetics and chemical industry. The invention also comprises a method for identifying red seed coat peanut raw materials independent of seed coat integrity, which is used for identifying or identifying the de-skinned peanut materials or other industrial and agricultural peanut materials, constructing a specific chromatographic analysis path based on the newly discovered specific chemical components in the red seed coat peanut materials, and identifying the red seed coat peanut raw materials based on the specific characteristic chromatographic peaks of the red seed coat peanut materials. As a preferable technical scheme of the invention, the method is at least suitable for peanut raw materials such as peanut protein powder, desquamation peanut powder and desquamation peanut kernels. As a preferred embodiment of the present invention, the specific chemical component is a single component or a plurality of components having extremely high antioxidant activity. As a preferred embodiment of the present invention, the specific chemical components include, but are not limited to, quercetin-3-O-rutinose-7-O-xylose/arabinoside. As a preferred technical solution of the present invention, the specific chromatographic analysis path specifically includes the following implementation steps: A. Naturally air-drying peanut materials to be tested until the water content is below 5%, selecting mature full and unbroken peanut kernels, grinding the peanut kernels by a grinder, and sieving the ground peanut kernels with a 20-mesh sieve to obtain sample powder to be tested; B. Preparing an extracting solution, namely, taking sample powder to be detected, namely, 0.1 g, precisely weighing, placing the sample powder into a2 mL centrifuge tube, adding n-hexane for degreasing treatment twice, precisely adding 50% methanol aqueous solution (V/V) according to the proportion of 1g to 15 mL (m/V), uniformly mixing, soaking 30min, performing ultrasonic auxiliary extraction at room temperature, 250 W,40 kHz,30 min, shaking every 10 min, centrifuging at room temperature, precisely measuring supernatant, and obtaining a primary extracting solution; C. Taking 1, 1-diphenyl-2-trinitrophenylhydrazine DPPH, precisely addi