CN-121978259-A - Centralized control method and system for column temperature boxes and control circuit of column temperature boxes

CN121978259ACN 121978259 ACN121978259 ACN 121978259ACN-121978259-A

Abstract

The invention relates to the technical field of liquid chromatograph, in particular to a centralized control method and system for a column temperature box and a control circuit for the column temperature box. The method comprises the steps that an upper computer obtains current temperature values of column temperature boxes in a plurality of liquid chromatograph in each group in real time through the Internet of things, the upper computer determines a difference distribution mode of the current temperature values of all column temperature boxes in the current group and preset target temperature values based on the current temperature values of the column temperature boxes in the plurality of liquid chromatograph in each group, if the difference distribution mode meets a first preset condition, the upper computer starts a centralized cooperative control mode, and if the difference distribution mode meets a second preset condition, the upper computer starts a distributed independent control mode. The invention can effectively reduce the control energy consumption cost while improving the temperature control efficiency of a plurality of liquid chromatographs, has an over-temperature protection function, and can automatically stop heating and alarm when detecting leakage so as to ensure safety.

Inventors

ZHOU LI
HUANG YINGZHANG
LIAO DAWEI

Assignees

重庆质谱科技有限公司

Dates

Publication Date: 20260505
Application Date: 20260206

Claims (10)

1. A column temperature box centralized control method based on the Internet of things is characterized in that a plurality of groups of liquid chromatographs are arranged in a first space, and correspondingly, the method further comprises the steps of: S101, an upper computer acquires current temperature values of a plurality of column temperature boxes in each group in real time through the Internet of things; S102, the upper computer determines a difference distribution mode of the current temperature values and preset target temperature values of all column temperature boxes in each group based on the current temperature values of the column temperature boxes in each group, and executes step S103 if the difference distribution mode meets a first preset condition, and executes step S104 if the difference distribution mode meets a second preset condition; The upper computer starts a centralized cooperative control mode, wherein the centralized cooperative control mode comprises that the upper computer generates a first PWM signal based on the maximum difference value and sends the first PWM signal to each column temperature box in a corresponding group, so that all the column temperature boxes in the corresponding group drive corresponding heating modules to heat based on the first PWM signal; And S104, the upper computer starts a distributed autonomous control mode, wherein the distributed autonomous control mode comprises the steps that the upper computer issues a control instruction, so that each column temperature box in a corresponding group generates respective second PWM signals according to the difference value between the respective current temperature value and the preset target temperature value so as to drive the corresponding heating module to heat.
2. The method for centralized control of column temperature boxes based on the Internet of things according to claim 1, wherein the first preset condition comprises that the difference value between the current temperature values and the preset target temperature values of all column temperature boxes in the same group is smaller than or equal to a first preset difference value.
3. The method for centrally controlling the column temperature boxes based on the Internet of things according to claim 2 is characterized in that the second preset condition comprises that the difference value between the current temperature value and the preset target temperature value of the column temperature boxes in the same group is larger than or equal to a second preset difference value N which is larger than or equal to a preset quantity threshold value M, and the second preset difference value is larger than the first preset difference value.
4. The column oven centralized control method based on the internet of things according to any one of claims 1 to 3, wherein a plurality of liquid chromatographs in the same group perform the same detection analysis task.
5. The method for centralized control of column temperature boxes based on the internet of things according to claim 4, further comprising the steps of, before starting the centralized cooperative control mode: if the deviation value between the difference value between the current temperature value of the P column temperature boxes in the same group and the preset target temperature value and the average difference value exceeds a preset threshold value, judging whether the environmental temperatures of all the column temperature boxes in the same group are the same or not based on the current positions of the P column temperature boxes; If the environmental temperature is the same and P is greater than or equal to A/2, converting the whole group into the distributed autonomous control mode, and prompting that P column incubators need to be maintained; If the environmental temperature is the same and P is less than A/2, prompting that P column incubators need to be maintained, wherein the whole group adopts the centralized cooperative control mode; if the environmental temperatures are different and the environmental temperatures in the P column incubators are lower or higher than the average environmental temperatures of other column incubators in the same group, dividing the P column incubators into one subgroup, and the other column incubators are the other subgroup, and starting a subgroup centralized cooperative control mode; if the environmental temperatures are different, and the environmental temperatures of P1 column incubators are lower than the average environmental temperatures of other column incubators in the same group, the environmental temperatures of P-P1 column incubators are higher than the average environmental temperatures of other column incubators in the same group, P1 column incubators are a subgroup, P-P1 column incubators are a subgroup, other column incubators in the same group are a subgroup, and each subgroup starts a subgroup centralized cooperative control mode.
6. The method for centralized control of column incubators based on the Internet of things according to claim 5, wherein the sub-group centralized cooperative control mode comprises the step that the upper computer generates a third PWM signal based on the maximum difference value in each sub-group to drive a heating module of the column incubators in the sub-group to heat.
7. The control circuit of the column temperature box in the liquid chromatograph is characterized by comprising a first temperature sensor for detecting the temperature of the column temperature box, a controller, a heater driving circuit and an Ethernet communication module, wherein the heater driving circuit and the Ethernet communication module are electrically connected with the controller; The controller is configured to acquire a current temperature value of the column temperature box from the first temperature sensor in real time, and send a first PWM signal which is received by the Ethernet communication module and issued by the upper computer to the heater driving circuit so as to drive the corresponding heating module to heat, or generate a corresponding second PWM signal according to a control instruction which is received by the Ethernet communication module and issued by the upper computer and is based on a difference value between the current temperature value and a preset target temperature value, and send the second PWM signal to the heater driving circuit so as to drive the corresponding heating module to heat; The first PWM signal is generated based on the maximum difference value when the upper computer determines that the distribution pattern of the difference values of the current temperature values of all column temperature boxes in the current group and the preset target temperature values meets a first preset condition based on the current temperature values of the column temperature boxes in the current group, and the control instruction is generated when the upper computer determines that the distribution pattern of the difference values of the current temperature values of all column temperature boxes in the current group and the preset target temperature values meets a second preset condition based on the current temperature values of the column temperature boxes in the current group.
8. The control circuit of the column incubator of the liquid chromatograph of claim 7, further comprising a liquid leakage detection module, wherein the output end of the liquid leakage detection module is connected with the controller through a third amplifier, and when the controller judges that liquid leakage occurs according to the data detected by the liquid leakage detection module, the controller controls the heater driving circuit to stop driving the heater to heat.
9. The control circuit for a column oven in a liquid chromatograph according to claim 8, further comprising a second temperature sensor for detecting a temperature of the tube, When the controller judges that the temperature abnormality occurs currently according to the temperature data of the second temperature sensor, the controller controls the over-temperature protection switch to be switched off so as to cut off the power supply of the heater; and/or, the first temperature sensor and the second temperature sensor adopt PT100.
10. A centralized monitoring system for a multi-liquid chromatograph, comprising: A plurality of liquid chromatographs comprising the control circuit of claims 7-9; the upper computer is configured to acquire current temperature values of column temperature boxes in the liquid chromatograph in real time, determine a difference distribution mode of the current temperature values of all column temperature boxes in the current area and preset target temperature values based on the current temperature values of the column temperature boxes in the liquid chromatograph in the current group, and start a centralized cooperative control mode if the difference distribution mode meets a first preset condition, wherein the centralized cooperative control mode comprises that the upper computer generates a first PWM signal based on the largest difference value and sends the first PWM signal to each column temperature box in the corresponding group so that all column temperature boxes in the corresponding group drive corresponding heating modules to heat based on the first PWM signal, and the upper computer starts a distributed autonomous control mode if the difference distribution mode meets a second preset condition, wherein the distributed autonomous control mode comprises that the upper computer sends a control instruction so that each column temperature box in the corresponding group generates respective second signals according to the difference value of the respective current temperature value and the preset target temperature value to drive the corresponding heating modules to heat.

Description

Centralized control method and system for column temperature boxes and control circuit of column temperature boxes Technical Field The invention relates to the technical field of liquid chromatograph, in particular to a centralized control method and system for a column temperature box and a control circuit for the column temperature box. Background In the technical field of traditional (liquid phase) chromatographs, a heating module and a refrigerating module are arranged in the chromatograph so that the temperature of a column temperature box reaches a target temperature, and the heating power of the heating module is dynamically adjusted according to a difference value between an actual temperature value and the target temperature value and a PID strategy. For example, patent application CN121090739a proposes a method, a system, an intelligent terminal and a storage medium for controlling the temperature of a chromatograph, which relate to the technical field of chromatograph sample separation, and include obtaining a sample type of a preset sample, analyzing the sample type to determine a window heating rate and a window target temperature, obtaining a chromatographic column initial temperature of a preset chromatographic column, analyzing the chromatographic column initial temperature and the window target temperature to determine a temperature to be raised of the chromatographic column, analyzing the chromatographic column temperature to be raised and the window heating rate to determine a chromatographic column heating time, and controlling the temperature of the chromatographic column according to the chromatographic column heating time and the window target temperature by a chromatographic column temperature regulating device. This application has the effect of improving the accuracy of the temperature retention time. For another example, a patent application with publication number CN120009452A proposes a temperature regulation method and device for ion chromatograph column temperature box, firstly, constructing a temperature change model based on Newton's law of cooling, after heating the heat source in the column temperature box, selecting three time points t < subgt, 1</subgt >, t < subgt >, 2</subgt >, t < subgt >, 3</subgt >, and making t < subgt, 3</subgt;t<subgt;2</subgt;=t<subgt;2</subgt; T < subgt >, 1</subgt >, and the corresponding cavity temperatures T < subgt >, 1</subgt >, T < subgt >, 2</subgt >, T < subgt >, and 3</subgt > of the temperature sensors in the column temperature box at three moments are recorded, a temperature model corresponding to each moment is built, the heat source is directly regulated according to the calculated heat source temperature by setting up a balance state equation for T < subgt >, 1</subgt >, T < subgt >, 2</subgt >, T < subgt >, 3</subgt >, the cavity temperature values at three moments T < subgt >, 1</subgt >, T < subgt >, 2</subgt >, T < subgt >, 3</subgt >, and the model formula taking logarithm and calculating to obtain the final cavity temperature value T < subgt, the final value subgt. The method reduces the waiting time after adjustment, shortens the time required by a temperature control system, directly obtains the temperature required to be set by the heating body, can finish the operation through one-time adjustment without repeated adjustment, thereby reducing the energy consumption and improving the working efficiency. For another example, a patent application with publication number CN114280183a proposes a temperature control device and method for a column oven of a liquid chromatograph, which relate to the technical field of liquid chromatography, and by respectively setting two temperature sensors inside and outside a temperature control region of the column oven, detecting the internal temperature and the ambient temperature of the temperature control region in real time, taking the internal temperature and the target temperature of the column oven as inputs for the ambient temperature, and controlling the output of peltier by using a PID algorithm by using control parameters and control modes corresponding to the ambient temperature. In the use of the column incubator, the control parameters and control modes are adaptively updated based on the environment temperature and the state after the control is stable. The invention improves the temperature control method of the column temperature box commonly used at present, improves the temperature control efficiency, reduces the time for reaching the temperature steady state, increases the control flexibility, and can adjust the temperature with the optimal PID parameters and control methods under various environmental temperatures so as to reach the optimal working state. Although these methods can allow the temperature of the column oven to reach the target temperature to some extent, the above solutions are all directed to single chromatograph control and are not suitable for centraliz