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CN-121978339-A - Dual-wavelength dry chemical detection method and system

CN121978339ACN 121978339 ACN121978339 ACN 121978339ACN-121978339-A

Abstract

The application provides a dual-wavelength dry chemical detection method and a system, wherein the method comprises the steps of obtaining a first reaction stable point signal of a first substance reaction area of a sample to be detected under a first wavelength and a second reaction stable point signal of the sample to be detected under a second wavelength; the method comprises the steps of inputting a first reaction stable point signal into a first concentration quantification model corresponding to a first substance to be detected to obtain a first concentration value, comparing the first concentration value with a preset concentration switching threshold value, determining a concentration detection result of the first substance to be detected in a sample to be detected based on the first concentration value if the first concentration value is smaller than the concentration switching threshold value, inputting a second reaction stable point signal into a second concentration quantification model corresponding to the first substance to be detected to obtain a second concentration value if the first concentration value is larger than or equal to the concentration switching threshold value, and determining the concentration detection result of the first substance to be detected in the sample to be detected based on the second concentration value. The application can improve the accuracy of the detection result of the dry chemical detection.

Inventors

  • LI YINGCHUN
  • CHENG HAOBO

Assignees

  • 北京理工大学

Dates

Publication Date
20260505
Application Date
20260205

Claims (10)

  1. 1. A dual wavelength dry chemistry detection method, the dual wavelength dry chemistry detection method comprising: Acquiring a first reaction stable point signal of a first substance to be detected reaction area of a sample to be detected under a first wavelength and a second reaction stable point signal of the first substance to be detected under a second wavelength; Inputting the first reaction stationary point signal into a first concentration quantization model corresponding to a first substance to be detected to obtain a first concentration value; comparing the first concentration value with a preset concentration switching threshold value in a numerical value manner; if the first concentration value is smaller than the concentration switching threshold value, determining a concentration detection result of a first substance to be detected in the sample to be detected based on the first concentration value; And if the first concentration value is greater than or equal to the concentration switching threshold, inputting the second reaction stable point signal into a second concentration quantization model corresponding to the first substance to be detected to obtain a second concentration value, and determining a concentration detection result of the first substance to be detected in the sample to be detected based on the second concentration value.
  2. 2. The method of claim 1, wherein the obtaining a first reaction plateau signal at a first wavelength for a first substance reaction zone of a sample to be tested comprises: collecting continuous optical signals of a first substance to be detected reaction area of the sample to be detected under a first wavelength, and converting the continuous optical signals into discrete time sequence signals; in a preset sliding time window, performing augmentation-Diyl-Fullerene test processing on the discrete time sequence signal to obtain a t statistical value; obtaining a probability value corresponding to the t statistical magnitude through table lookup; and taking the discrete time sequence signal at the moment when the probability value is smaller than a preset significance level threshold value as the first reaction stationary point signal.
  3. 3. The dual wavelength dry chemistry detection method according to claim 2, wherein said performing an augmented di-fullerene test process on said discrete time series signal within a predetermined sliding time window to obtain a t statistical value comprises: Acquiring a regression equation containing a displacement term, a time trend term and a random error term; performing regression analysis on the regression equation by using the discrete time series signals in the sliding time window; determining an estimated value of a unit root existence coefficient in the regression equation and a standard deviation of the estimated value; The t-statistic is determined based on a ratio of the estimate to the standard deviation.
  4. 4. The dual wavelength dry chemistry detection method of claim 1, wherein said first concentration quantification model is generated by: obtaining a plurality of first standard solutions of a first substance to be detected, wherein the concentrations of the first substance to be detected in different first standard solutions are different; determining a first standard concentration value of each first standard solution by using a preset first standard detection device; Acquiring a first calibration reaction stationary point signal of each first standard solution under a first wavelength; and generating the first concentration quantification model based on the first calibration reaction stationary point signal and the corresponding first standard concentration value.
  5. 5. The method of claim 4, wherein after determining the first standard concentration value of each of the first standard solutions using a predetermined first standard detection device, further comprising: Acquiring a second calibration reaction stationary point signal of each first standard solution under a second wavelength; And generating the second concentration quantification model based on the second calibration reaction stationary point signal and the corresponding first standard concentration value.
  6. 6. The dual wavelength dry chemistry detection method according to claim 1, wherein the step of acquiring a first reaction plateau signal at a first wavelength and a second reaction plateau signal at a second wavelength for a first substance reaction zone of a sample to be detected is performed simultaneously with the step of acquiring a first reaction plateau signal at a first wavelength, further comprising: acquiring a third reaction stable point signal of a second substance reaction area of the sample to be detected under a second wavelength; The dual wavelength dry chemical detection method further comprises the following steps: Inputting the third reaction stationary point signal into a third concentration quantization model corresponding to a second substance to be detected to obtain a third concentration value; and determining a concentration detection result of the second substance to be detected in the sample to be detected based on the third concentration value.
  7. 7. The dual wavelength dry chemistry detection method of claim 6, wherein said third concentration quantification model is generated by: Obtaining a plurality of second standard solutions of second substances to be detected, wherein the concentrations of the second substances to be detected in the second standard solutions are different; Determining a second standard concentration value of each second standard solution by using a preset second standard detection device; acquiring a third calibration reaction stationary point signal of each second standard solution under a second wavelength; and generating the third concentration quantization model based on the second standard concentration value and the corresponding third calibration reaction stationary point signal.
  8. 8. The dual wavelength dry chemical detection method according to any one of claims 1to 7, wherein the first test substance is blood glucose and the second test substance is hemoglobin.
  9. 9. The dual-wavelength dry biochemical measurement system is characterized by comprising a first photoelectric detection module and a data analysis module, wherein the first photoelectric detection module comprises a first light source corresponding to a first wavelength, a second light source corresponding to a second wavelength and an optical signal receiving unit, and the data analysis module is used for: The dual wavelength dry chemical detection method according to any one of claims 1 to 8 is performed based on the optical signal at the first wavelength and the optical signal at the second wavelength collected by the optical signal receiving unit.
  10. 10. The dual wavelength dry biochemical measurement system according to claim 9, further comprising a second photo detection module and a sample receiving space, The sample accommodating space is used for accommodating a sample to be tested, a first detection hole corresponding to a first substance to be tested reaction zone of the sample to be tested and a second detection hole corresponding to a second substance to be tested reaction zone of the sample to be tested are arranged on the side wall of the shell forming the sample accommodating space, The photoelectric detection direction of the first photoelectric detection module faces the first detection hole, and the photoelectric detection direction of the second photoelectric detection module faces the second detection hole.

Description

Dual-wavelength dry chemical detection method and system Technical Field The application relates to the technical field of dry chemical detection, in particular to a dual-wavelength dry chemical detection method and system. Background The dry chemical detection technology is widely applied to the field of clinical and household instant test (Point-of-CARE TESTING, POCT) because of the advantages of reagent immobilization, no liquid flow path, convenient operation and the like, and particularly aims at daily blood sugar and hemoglobin monitoring of patients with diabetes and complications thereof. The basic principle of the technology is that a photoelectric detection system is utilized to measure the color depth change after the chemical reaction of a blood sample and a solid-phase reagent on a test strip, and the concentration of a substance to be detected in blood is calculated by inversion through detecting the intensity of a reflected light signal under a specific wavelength. However, in a high concentration sample detection scenario, the color depth of a portion of the reaction product may increase significantly, resulting in a physical shift of its spectral maximum absorption peak, while the dark color reactant causes the photosensor to enter the nonlinear response region and even tends to saturate. The change of the optical characteristics makes the conventional gain amplification of the electric signal unable to restore the real light absorption condition from the physical aspect, so that the signal resolution in the high concentration interval is greatly reduced, and the accuracy of the detection result is seriously affected. Disclosure of Invention The embodiment of the application provides a dual-wavelength dry chemical detection method and a dual-wavelength dry chemical detection system, aiming at improving the accuracy of a detection result of dry chemical detection. In a first aspect, embodiments of the present application provide a dual wavelength dry chemistry detection method, the dual wavelength dry chemistry detection method comprising: Acquiring a first reaction stable point signal of a first substance to be detected reaction area of a sample to be detected under a first wavelength and a second reaction stable point signal of the first substance to be detected under a second wavelength; Inputting the first reaction stationary point signal into a first concentration quantization model corresponding to a first substance to be detected to obtain a first concentration value; comparing the first concentration value with a preset concentration switching threshold value in a numerical value manner; if the first concentration value is smaller than the concentration switching threshold value, determining a concentration detection result of a first substance to be detected in the sample to be detected based on the first concentration value; And if the first concentration value is greater than or equal to the concentration switching threshold, inputting the second reaction stable point signal into a second concentration quantization model corresponding to the first substance to be detected to obtain a second concentration value, and determining a concentration detection result of the first substance to be detected in the sample to be detected based on the second concentration value. In the above embodiment, by comparing the first concentration value obtained based on the first reaction stationary point signal with the concentration switching threshold value, the first concentration value is directly adopted when the first concentration value is smaller than the concentration switching threshold value to determine that the sample to be detected is low in concentration, and the second concentration value obtained based on the second reaction stationary point signal and the second concentration quantization model is adopted when the first concentration value is greater than or equal to the concentration switching threshold value, so that the optimal wavelength signal and the quantization model are automatically matched for different concentration intervals of the sample to be detected to calculate, and errors caused by nonlinear response of the single wavelength signal under high concentration are eliminated, thereby improving the accuracy of the detection result of dry chemical detection. In an embodiment, the obtaining the first reaction plateau signal of the first substance reaction area of the sample to be measured at the first wavelength includes: collecting continuous optical signals of a first substance to be detected reaction area of the sample to be detected under a first wavelength, and converting the continuous optical signals into discrete time sequence signals; in a preset sliding time window, performing augmentation-Diyl-Fullerene test processing on the discrete time sequence signal to obtain a t statistical value; obtaining a probability value corresponding to the t statistical magnitud