CN-121978349-A - Rapid detection method for specific biomarker of central girl sexual precocity

CN121978349ACN 121978349 ACN121978349 ACN 121978349ACN-121978349-A

Abstract

The invention relates to the technical field of biomedical detection, in particular to a rapid detection method for a specific biomarker of central girl sexual precocity. When a blood sample is detected, an original light intensity sequence of a chromatographic test strip is obtained and adjusted to be a standardized net signal sequence, the width of a color band is controlled by the chromatographic flow rate, the fluid diffusion level under the viscosity of the sample can be reflected, the reference width of a quality control line and the sharpness of a waveform are determined according to the control line, the waveform distribution characteristic of the sample is obtained, the light intensity of a quality control and interference capturing interval is distorted due to the increase of the viscosity of the blood, when the light intensity of the interference capturing interval meets preset conditions, a saturation evaluation index is determined according to the peak shape characteristic and the quality control line related parameters of the interference capturing interval, the saturation degree is evaluated, the light intensity is analyzed in a membrane gap interval to obtain a background tailing ratio, the leakage flux of free interferents is quantized, and finally, the effective value of the blood sample is obtained by fusing the saturation evaluation index and the background tailing ratio, the condition of the sample can be comprehensively and accurately reflected, and whether the sample is suitable for detection is determined by assistance.

Inventors

ZHAO SHUAI
SUN FENGPING
ZHANG MAN
ZHANG ZHENGYANG
WANG LUYU

Assignees

河南省医学科学院

Dates

Publication Date: 20260505
Application Date: 20260206

Claims (10)

1. A method for rapid detection of a specific biomarker of central girl sexual precocity, the method comprising: When a blood sample is detected, an original light intensity sequence on a chromatographic test strip is obtained, and the original light intensity sequence is adjusted, so that a standardized net signal sequence is obtained; determining the waveform sharpness of the quality control line according to the distribution characteristics of the light intensity in the standardized net signal sequence; When the light intensity in the interference capturing interval in the standardized net signal sequence meets the preset condition, determining a saturation evaluation index of the interference capturing interval based on the peak shape characteristic, the quality control line reference width and the quality control line waveform sharpness of the light intensity in the interference capturing interval; And fusing the saturation evaluation index and the background tailing ratio by using a preset saturation term weight, a leakage term weight and a coupling term weight to obtain a blood sample effective value, and judging the validity of the blood sample based on the blood sample effective value.
2. The rapid detection method of specific biomarkers for central girl sexual precocity according to claim 1, wherein the method for obtaining normalized net signal sequence comprises: In the quality control interval, marking an index corresponding to the maximum light intensity value as a quality control line center index; in the original light intensity sequence, taking the difference value between the index of each sampling position and the center index of the quality control line as the index of each sampling position in the standardized net signal sequence; And taking the average value of all light intensities in the background noise interval as a film background baseline value, setting the light intensity of a certain sampling position in the standardized net signal sequence as 0 when the light intensity of the sampling position is smaller than or equal to the film background baseline value, otherwise taking the difference value of the light intensity of the sampling position and the film background baseline value as the light intensity of the sampling position in the standardized net signal sequence.
3. The rapid detection method of specific biomarkers for central girl sexual precocity according to claim 2, wherein the method for obtaining the reference width of the quality control line comprises the steps of: In the standardized net signal sequence, traversing the index at the center of the quality control line to two sides respectively, and marking the index with the light intensity smaller than or equal to half of the peak value for the first time as a boundary index; And taking the product of the absolute value of the difference value of the two boundary indexes and the step length as the reference width of the quality control line.
4. The rapid detection method for specific biomarkers for central girl sexual precocity according to claim 3, wherein the method for obtaining the sharpness of the waveform of the quality control line comprises the steps of: In the normalized net signal sequence, taking two boundary indexes and light intensities corresponding to the indexes between the boundary indexes as subsequences; In the subsequence, kurtosis of all light intensities is calculated as the quality control line waveform sharpness.
5. The rapid detection method of specific biomarkers for central girl sexual precocity according to claim 1, wherein the method for obtaining the saturation evaluation index comprises: Taking the product of the reference width of the quality control line and a preset rheological position compensation coefficient as a width contrast value; Intercepting a sequence of an interference capturing interval from a standard net signal sequence as a target sequence; In the target sequence, acquiring the full width half maximum of the maximum peak in the waveform, setting a physical width residual error to 0 when the full width half maximum is smaller than the width contrast value, otherwise setting the physical width residual error to be the difference value of the full width half maximum and the width contrast value; in the target sequence, analyzing peak shape characteristics and comparing the peak shape characteristics with the sharpness of the quality control line waveform, so as to determine a collapse index; taking the product of the square of the collapse index and a preset form sensitivity coefficient as a distortion factor, and taking the sum of the distortion factor and a preset constant as a distortion penalty value; and taking the value obtained by normalizing the distortion penalty value and the sum value of the physical width residual errors as a saturation evaluation index of the interference capturing interval.
6. The rapid detection method of specific biomarkers for central girl sexual precocity according to claim 5, wherein the method for obtaining collapse indicators comprises: In the target sequence, taking an index corresponding to a maximum light intensity value as an interference line center index, taking the ratio of the full width at half maximum to the step length as an index width contrast value by rounding upwards, taking the difference value of the interference line index and the index width contrast value as an index boundary value, taking the sum value of the interference center index and the index width contrast value as an index boundary value, and calculating the kurtosis of the light intensities corresponding to the two index boundary values and the index between the two index boundary values as actual measurement sharpness of the interference line; and when the sharpness of the quality control line waveform is smaller than the actually measured sharpness of the interference line, setting the collapse index to 0, otherwise, taking the difference value between the sharpness of the quality control line waveform and the actually measured sharpness of the interference line as the collapse index.
7. The rapid detection method of specific biomarkers for central girl sexual precocity according to claim 1, wherein the method for obtaining the background tailing ratio comprises: And in the membrane gap interval, acquiring a normalized value of the average value of the light intensity at all sampling points in the normalized net signal sequence as a background tailing ratio.
8. The rapid detection method for specific biomarkers for central girl sexual precocity according to claim 1, wherein the method for obtaining effective values of blood samples comprises: Taking the product of the saturation evaluation index and the background tailing ratio as a coupling factor; Multiplying the preset saturation term weight with the saturation evaluation index, multiplying the preset leakage term weight with the background tailing ratio, multiplying the preset coupling term weight with the coupling factor, and carrying out negative correlation mapping on the sum value of the three products and normalizing the sum value to obtain the blood sample effective value.
9. The rapid detection method for specific biomarkers for central girl sexual precocity according to claim 1, wherein said determining the validity of the blood sample based on said blood sample validity value comprises: and when the effective value of the blood sample is smaller than or equal to a preset system safety threshold value, judging the blood sample as an invalid sample, otherwise, judging the blood sample as an valid sample.
10. The rapid detection method of specific biomarkers for central girl sexual precocity according to claim 2, wherein standard deviation of all light intensities in a background noise interval is used as background noise background value, and the preset condition is: the maximum light intensity value in the normalized net signal sequence in the interference capturing interval is more than or equal to 3 times background noise background value.

Description

Rapid detection method for specific biomarker of central girl sexual precocity Technical Field The invention relates to the technical field of biomedical detection, in particular to a rapid detection method for a specific biomarker of central girl sexual precocity. Background The central girl sexual precocity is an endocrine disease which seriously affects the growth and development of children and the physical and psychological health, and accurately and quickly detects the central girl sexual precocity, is important for early diagnosis, timely intervention and treatment, and can effectively avoid a series of adverse consequences such as short stature, psychological disorder and the like caused by sexual precocity. Early screening of Central Precocious Puberty (CPP) relies on the detection of trace polypeptide markers such as the gonadotrophin releasing hormone upstream regulator kisspptin in peripheral blood. Because of poor compliance of venous blood sampling of children, the rapid immunochromatography detection by utilizing fingertip whole blood has important clinical application value. The chromatographic test strip detection technology is a rapid, gradual change and low-cost monitoring method, is widely applied in the biomedical field, and usually judges the result only through a light intensity threshold value or a simple background deduction method when the prior art detects the blood sample of girls, but because the blood viscosity of different children patients is greatly different, and different eosinophils or rheumatoid factors exist in part of children patients, the interferents are easy to be subjected to nonspecific adsorption, so that the effectiveness of the blood sample is reduced, and the final judging result is seriously influenced. Disclosure of Invention In order to solve the technical problems that the validity of a blood sample is reduced and the final interpretation result is seriously influenced because different children patients have large blood viscosity differences and different eosinophilic antibodies or rheumatoid factors exist in part of children patients, the invention aims to provide a rapid detection method for specific biomarkers of central girl sexual precocity, which adopts the following specific technical scheme: When a blood sample is detected, an original light intensity sequence on a chromatographic test strip is obtained, and the original light intensity sequence is adjusted, so that a standardized net signal sequence is obtained; determining the waveform sharpness of the quality control line according to the distribution characteristics of the light intensity in the standardized net signal sequence; When the light intensity in the interference capturing interval in the standardized net signal sequence meets the preset condition, determining a saturation evaluation index of the interference capturing interval based on the peak shape characteristic, the quality control line reference width and the quality control line waveform sharpness of the light intensity in the interference capturing interval; And fusing the saturation evaluation index and the background tailing ratio by using a preset saturation term weight, a leakage term weight and a coupling term weight to obtain a blood sample effective value, and judging the validity of the blood sample based on the blood sample effective value. Further, the method for acquiring the normalized net signal sequence comprises the following steps: In the quality control interval, marking an index corresponding to the maximum light intensity value as a quality control line center index; in the original light intensity sequence, taking the difference value between the index of each sampling position and the center index of the quality control line as the index of each sampling position in the standardized net signal sequence; And taking the average value of all light intensities in the background noise interval as a film background baseline value, setting the light intensity of a certain sampling position in the standardized net signal sequence as 0 when the light intensity of the sampling position is smaller than or equal to the film background baseline value, otherwise taking the difference value of the light intensity of the sampling position and the film background baseline value as the light intensity of the sampling position in the standardized net signal sequence. Further, the method for obtaining the quality control line reference width comprises the following steps: In the standardized net signal sequence, traversing the index at the center of the quality control line to two sides respectively, and marking the index with the light intensity smaller than or equal to half of the peak value for the first time as a boundary index; And taking the product of the absolute value of the difference value of the two boundary indexes and the step length as the reference width of the quality control line. Further, the method for o