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CN-121978782-A - Construction method of three-dimensional laminated chiral nano composite structure based on DNA paper folding template

CN121978782ACN 121978782 ACN121978782 ACN 121978782ACN-121978782-A

Abstract

The invention aims to provide a construction method of a three-dimensional laminated chiral nano composite structure based on a DNA paper folding template, which comprises the steps of carrying out hydrophilic treatment on a silicon substrate by using a plasma cleaning machine, designing and synthesizing the DNA paper folding template which is provided with a plurality of symmetrically distributed capture sites, respectively adopting a DNA modified gold nano disc and a gold nano ball, spreading the DNA paper folding template on the surface of the treated substrate, firstly hybridizing the DNA modified gold nano disc with the template, fixing the DNA modified gold nano disc at the central position of the DNA paper folding, hybridizing the DNA modified gold nano ball with a DNA chain on the gold nano disc to form the three-dimensional laminated structure, and cleaning unbound nano particles to obtain the target composite structure. According to the invention, accurate positioning of the gold nano disc and the gold nano ball in a three-dimensional space and ordered stacking in the vertical direction are realized through the DNA paper folding template, and a chiral nano composite structure with a three-dimensional lamination effect is constructed.

Inventors

  • LAN XIANG
  • SONG JUN

Assignees

  • 东华大学

Dates

Publication Date
20260505
Application Date
20260203

Claims (10)

  1. 1. The construction method of the three-dimensional laminated chiral nano composite structure based on the DNA paper folding template is characterized by comprising the following steps: Hydrophilic treatment is carried out on the silicon substrate by utilizing a plasma cleaning machine; Designing and synthesizing a DNA paper folding template which is provided with a plurality of symmetrically distributed capture sites; Respectively modifying the gold nano disc and the gold nano ball by DNA; Spreading the DNA paper folding template on the surface of the treated substrate; firstly, hybridizing a gold nano disc modified by DNA with a template, and fixing the gold nano disc and the template at a central position; Hybridizing the DNA modified gold nanospheres with a capturing chain above the template to form a three-dimensional laminated structure; And cleaning the unbound nano particles to obtain the chiral nano composite structure with the three-dimensional lamination effect.
  2. 2. The method for constructing a three-dimensional stacked chiral nanocomposite structure according to claim 1, wherein the basic structural units of the DNA origami template are triangular DNA tiles, and are orderly connected by means of DNA edge chains, and the geometric dimensions of the triangular DNA tiles are 80nm on a side and 2nm thick.
  3. 3. The method for constructing a three-dimensional stacked chiral nanocomposite structure according to claim 2, wherein the triangular DNA tiles are composed of 7259 base long single-stranded DNA derived from m13mp18 virus, a set of auxiliary strand DNA and capturing strand DNA, the auxiliary strand DNA and capturing strand DNA are subjected to base pairing and folded to form a tile structure, the auxiliary strand DNA and capturing strand DNA are total 168, the number of bases of each auxiliary strand is allowed to be unequal, the capturing strand DNA comprises two short sequences, an S1 sequence participates in pairing of long-stranded DNA, an S2 sequence is used as a sticky end for binding a nano disk or a nano rod, and 24 DNA edge strands are additionally provided for connecting each triangular DNA tile into a DNA paper folding template.
  4. 4. A method of constructing a three-dimensional stacked chiral nanocomposite structure according to claim 3, wherein the superlattice structure with a predetermined geometric arrangement is constructed by designing and adjusting the spatial distribution of the capturing chains on the triangular DNA tiles.
  5. 5. The method of constructing a three-dimensional stacked chiral nanocomposite structure according to claim 4, wherein 16 capture chains are required for any one of the nanorods in the superlattice structure, each gold nanosphere is immobilized by at least 2 capture chains, and each gold nanosphere is assembled vertically by hybridization with a capture chain above the gold nanosphere.
  6. 6. The method of constructing a three-dimensional stacked chiral nanocomposite structure according to claim 1, wherein the DNA folding template is spread on the surface of the silica substrate, allowed to stand for 20 minutes to stably bind the folding template to the substrate, and then washed with a buffer solution of 1×tae-Mg 2+ to remove unbound DNA strands.
  7. 7. The method for constructing a three-dimensional stacked chiral nanocomposite structure according to claim 1, wherein DNA used to modify the nanodiscs, nanorods, and nanospheres is a DNA sequence with thiol modification.
  8. 8. The method for constructing a three-dimensional stacked chiral nanocomposite structure according to claim 1 or 7, wherein the nanodiscs are gold nanodiscs, the nanorods are gold nanorods, and the nanospheres are gold nanospheres.
  9. 9. The method for constructing a three-dimensional stacked chiral nanocomposite structure according to claim 1, further comprising a step of removing excess DNA after finishing DNA modification of the nanodisk, the nanorod, and the nanosphere, wherein the step is performed by 2% agarose gel electrophoresis to purify the modified nanomaterial.
  10. 10. The method for constructing a three-dimensional stacked chiral nanocomposite structure according to claim 1, wherein when hybridizing the DNA-modified nanodiscs and nanorods with the DNA folding template, the hybridization temperature is controlled to be lower than the melting temperature of the DNA folding template.

Description

Construction method of three-dimensional laminated chiral nano composite structure based on DNA paper folding template Technical Field The invention belongs to the field of nano materials and nano photonics, and particularly relates to a method for constructing a three-dimensional laminated chiral nano composite structure based on a DNA paper folding template. Background The development history of DNA nanotechnology clearly demonstrates the evolution path of this field from concept sprouting to structural refinement. In 1982, seeman taught the promise of building ordered nanostructures using branched DNA molecules with complementary ends, laying the foundation for rational assembly using DNA base pairing. Thereafter, researchers developed more robust modules such as double-crossover (DX) building blocks, successfully achieved periodic two-dimensional lattice self-assembly, verifying the feasibility of DNA as a nanostructure scaffold. A major breakthrough in technology occurred in 2006, and the "DNA origami" taught by Rothemund has completely changed the domain layout. The method utilizes the fixed-point hybridization of one long-chain DNA and hundreds of short chains, and can efficiently and programmably fold complex two-dimensional nano patterns such as rectangles, triangles and even stars (Stars). The DNA paper folding operation provides a universal rigid template platform for subsequent functional integration by virtue of excellent positioning precision (5 nm) and rich shape libraries. On this basis, research focus has shifted to the use of DNA origami templates to guide the precise alignment of inorganic nanoparticles. Early work focused primarily on assembling single types of nanoparticles (e.g., gold nanospheres) in a two-dimensional plane to construct periodic arrays. Subsequently, to introduce optical chirality, we began to try to simultaneously locate isotropic nanospheres and anisotropic nanorods (e.g., gold nanorods) on a paper folding template, and successfully construct a two-dimensional chiral superlattice with a pronounced circular dichroism response by carefully designing the relative positions and orientations of the two in-plane. These structures of "in-plane" arrangement fully demonstrate the powerful ability of DNA self-assembly in modulating nanoparticle spatial arrangement to produce novel collective optical properties. However, with the intensive research, the limitation of chiral structures in two-dimensional planes is also gradually revealed, namely the optical chiral signal intensity and the modulation freedom degree are often limited by plane geometric constraints. Recently, leading-edge exploration began to point to the third dimension, namely building three-dimensional stacked nanosystems with vertically stacked configurations. Such structures are expected to couple spatially through the interaction of strong electromagnetic fields generated by the vertical stacks, resulting in chiral optical responses of far hyperplane structures. However, achieving this objective faces serious challenges in that it requires DNA templates that not only can be precisely positioned in-plane, but also have the ability to capture different nanoparticles stepwise and specifically in the vertical direction, and at the same time, how to stably construct a vertical heterostructure based on anisotropic nanoplatelets (e.g., gold nanoplatelets) remains an unsolved challenge. Disclosure of Invention The invention aims to overcome the defects of the prior art and provides a method for constructing a three-dimensional laminated chiral nano composite structure based on a DNA paper folding template. In order to achieve the aim of the invention, the invention adopts the following technical scheme: A method for constructing a three-dimensional laminated chiral nano composite structure based on a DNA paper folding template comprises the following steps of carrying out hydrophilic treatment on a silicon substrate by utilizing a plasma cleaning machine, designing and synthesizing the DNA paper folding template which is provided with a plurality of symmetrically distributed capture sites, respectively modifying a gold nano disc and gold nano balls by DNA, spreading the DNA paper folding template on the surface of the treated substrate, hybridizing the DNA modified gold nano disc with the template, fixing the DNA modified gold nano disc at a central position, hybridizing the DNA modified gold nano balls with a capture chain above the template to form the three-dimensional laminated structure, and cleaning unbound nano particles to obtain the chiral nano composite structure with a three-dimensional laminated effect. By adopting the technical scheme, the invention has the beneficial effects that: The invention provides a three-dimensional laminated chiral nano composite structure based on a DNA paper folding template, which comprises the following steps of carrying out hydrophilic treatment on a silicon substrate by ut