CN-121981956-A - Microsphere image processing method and device and computer equipment

CN121981956ACN 121981956 ACN121981956 ACN 121981956ACN-121981956-A

Abstract

The embodiment of the application is suitable for the fields of biomedical technology and image processing technology, and provides a microsphere image processing method, device, system and computer equipment, wherein the method comprises the steps of respectively acquiring microsphere images of the lightened microspheres according to a plurality of rounds under the condition that a plurality of microspheres on a microsphere chip are lightened by a plurality of fluorescence channels excited by fluorescence signals; the method comprises the steps of respectively splicing a plurality of microsphere images acquired by each round into panoramic images under the corresponding round, aligning the plurality of panoramic images under the plurality of rounds to obtain a target microsphere image, and identifying each microsphere contained in the target microsphere image. By adopting the method, each microsphere contained in the microsphere image can be accurately identified and marked.

Inventors

He Zhuoying
LUO YANDE
ZHANG FENG

Assignees

利德健康科技(广州)有限公司

Dates

Publication Date: 20260505
Application Date: 20251225

Claims (10)

1. A microsphere image processing method, comprising: under the condition that a plurality of fluorescent channels excite fluorescent signals to lighten a plurality of microspheres on a microsphere chip, respectively acquiring microsphere images of the lightened microspheres according to a plurality of rounds, wherein each microsphere carries a plurality of bases; Respectively splicing a plurality of microsphere images acquired by each round into panoramic images under the corresponding rounds; aligning a plurality of panoramic images under a plurality of rounds to obtain a target microsphere image; Each of the microspheres contained in the target microsphere image is identified.
2. The method of claim 1, wherein the separately capturing microsphere images of the lit microspheres in a plurality of passes comprises: Under any round, continuously acquiring images of the microsphere chip according to a preset scanning path to obtain microsphere images corresponding to all areas on the microsphere chip one by one, wherein overlapping areas are arranged between the microsphere images corresponding to adjacent areas; And repeatedly carrying out image acquisition of a plurality of rounds to obtain a plurality of microsphere images under the plurality of rounds.
3. The method according to claim 2, wherein the step of continuously acquiring images of the microsphere chip according to a preset scanning path to obtain microsphere images corresponding to each region of the microsphere chip one by one in any turn comprises: Under any round, respectively carrying out image acquisition on the same area on the microsphere chip to obtain a plurality of multichannel images of the area, wherein the number of the multichannel images is equal to that of the fluorescent channels; And repeatedly executing the image acquisition operation on each region on the microsphere chip to obtain a plurality of multi-channel images of each region.
4. A method according to any one of claims 1 to 3, wherein the microsphere image comprises a plurality of multi-channel images corresponding to each region on the microsphere chip, and the stitching the plurality of microsphere images acquired in each round into a panoramic image under the corresponding round comprises: registering a plurality of multichannel images corresponding to each region on the microsphere chip respectively to obtain multichannel alignment images of each region; and splicing the multichannel aligned images of each region under each round into a panoramic image under the corresponding round.
5. The method of claim 4, wherein stitching the multi-channel aligned image for each region under each pass into a panoramic image under the respective pass comprises: Under any round, determining overlapping images in the multichannel aligned images corresponding to the adjacent areas; registering the overlapped images between the adjacent areas, and splicing the multi-channel alignment images corresponding to the adjacent areas according to registration results.
6. The method of any one of claims 1 to 3 or 5, wherein said identifying each of said microspheres contained in said target microsphere image comprises: Performing edge detection on the target microsphere image to obtain a microsphere edge image, and performing image segmentation on the microsphere edge image to obtain a microsphere segmentation image; generating a target image to be detected based on the microsphere edge image and the microsphere segmentation image; and carrying out connected domain detection on the target image to be detected, and identifying each microsphere contained in the image based on a connected domain detection result.
7. The method according to claim 6, wherein the identifying each of the microspheres contained in the image based on the connected domain detection result comprises: Filling the connected domain of the non-characterization microsphere according to the detection result of the connected domain; performing Euclidean distance transformation on the filled target image to be detected to obtain a distance map from each foreground pixel to the nearest background pixel; Detecting local maximum points of the distance graph to obtain a plurality of peak points, wherein any peak point is used for representing the center point of the microsphere to be identified; And marking each microsphere according to each peak point.
8. The method of claim 6, wherein generating the target image to be detected based on the microsphere edge image and the microsphere segmentation image comprises: And subtracting the microsphere edge image from the microsphere segmentation image to obtain the target image to be detected.
9. A computer device comprising a memory, a processor and a computer program stored in the memory and executable on the processor, characterized in that the computer device is caused to implement the method of any one of claims 1 to 8 when the processor executes the computer program.
10. A computer program product comprising a computer program which, when run, causes the method of any one of claims 1 to 8 to be performed.

Description

Microsphere image processing method and device and computer equipment Technical Field The embodiment of the application belongs to the technical field of biomedical technology and image processing, and particularly relates to a microsphere image processing method, a microsphere image processing device and computer equipment. Background In space transcriptomics, to obtain spatial information, bases are typically bound to microspheres such that the microspheres bear fluorescent groups and blocking groups. The fluorescent signal is then excited by a laser, for example by four fluorescent channels (CY 5 channel, FITC channel, ROX channel, TRITC channel), so that the microsphere image can be captured by a charge coupled device (charge coupled device, CCD) camera. Since the bases bound to the microspheres are not identical, microspheres that bind different bases will light up on different fluorescent channels. Typically, in idle running experiments, the microsphere chip will be filled with tens of thousands of microspheres. For example, a microsphere chip in practical application is generally covered with 30 to 50 tens of thousands of microspheres. Because each microsphere carries different base information, the excitation fluorescent signals of the corresponding channels are difficult to control in a targeted manner, so that each microsphere is lightened on the corresponding fluorescent channel, and each microsphere on the chip cannot be accurately identified. Therefore, how to accurately identify each microsphere for a large number of microspheres spread on a microsphere chip is one of the problems that those skilled in the art need to solve. Disclosure of Invention In view of the above, embodiments of the present application provide a method, an apparatus, a system, and a computer device for processing a microsphere image, which are used for accurately identifying and marking each microsphere contained in the microsphere image. A first aspect of an embodiment of the present application provides a microsphere image processing method, including: under the condition that a plurality of fluorescent channels excite fluorescent signals to lighten a plurality of microspheres on a microsphere chip, respectively acquiring microsphere images of the lightened microspheres according to a plurality of rounds, wherein each microsphere carries a plurality of bases; Respectively splicing a plurality of microsphere images acquired by each round into panoramic images under the corresponding rounds; aligning a plurality of panoramic images under a plurality of rounds to obtain a target microsphere image; Each of the microspheres contained in the target microsphere image is identified. Optionally, the capturing microsphere images of the lit microsphere according to a plurality of rounds includes: Under any round, continuously acquiring images of the microsphere chip according to a preset scanning path to obtain microsphere images corresponding to all areas on the microsphere chip one by one, wherein overlapping areas are arranged between the microsphere images corresponding to adjacent areas; And repeatedly carrying out image acquisition of a plurality of rounds to obtain a plurality of microsphere images under the plurality of rounds. Optionally, under any turn, performing continuous image acquisition on the microsphere chip according to a preset scanning path to obtain microsphere images corresponding to each region on the microsphere chip one by one, including: Under any round, respectively carrying out image acquisition on the same area on the microsphere chip to obtain a plurality of multichannel images of the area, wherein the number of the multichannel images is equal to that of the fluorescent channels; And repeatedly executing the image acquisition operation on each region on the microsphere chip to obtain a plurality of multi-channel images of each region. Optionally, the microsphere image includes a plurality of multichannel images corresponding to each region on the microsphere chip, and the stitching the plurality of microsphere images collected in each round into panoramic images under the corresponding round includes: registering a plurality of multichannel images corresponding to each region on the microsphere chip respectively to obtain multichannel alignment images of each region; and splicing the multichannel aligned images of each region under each round into a panoramic image under the corresponding round. Optionally, the stitching the multi-channel aligned image of each region under each round into the panoramic image under the corresponding round includes: Under any round, determining overlapping images in the multichannel aligned images corresponding to the adjacent areas; registering the overlapped images between the adjacent areas, and splicing the multi-channel alignment images corresponding to the adjacent areas according to registration results. Optionally, the identifying each of the microspheres con