CN-121985884-A - Aflatoxin production inhibitor, aflatoxin production inhibitor produced by aflatoxin production inhibitor, process for producing the same, and method for controlling aflatoxin contamination using the same

Abstract

The present invention provides a novel strain which is a novel microorganism having a high activity for inhibiting the production of aflatoxin, an aflatoxin production inhibitor comprising the novel strain, a method for effectively producing an aflatoxin production inhibitor comprising an agent for improving the effect thereof, and a method for controlling aflatoxin contamination using the aflatoxin production inhibitor or the aflatoxin production inhibitor. The aflatoxin production inhibitor comprises a culture of Klebsiella (Klebsiella sp.) or ralstonia (Raoultella sp.) or a compound represented by the following general formula (1). In the formula (1), R is a linear or branched alkyl group having 1 to 4 carbon atoms.

Inventors

• SHOHEI SAKUDA
• UCHIDA KENICHI
• Kiko Hakojima
• SHI YONGHAI
• SAKODA Ayaka
• Suh Chang Chang
• TERADA SHINJI

Assignees

• 学校法人帝京大学

Dates

Publication Date

20260505

Application Date

20240927

Priority Date

20230928

Claims (8)

1. 1. An aflatoxin production inhibitor is provided, it comprises Klebsiella sp ) Or Raoultella genus ) Is a culture of (a) a strain of (b).
2. 2. An aflatoxin production inhibitor comprising a compound represented by the following general formula (1), [ Chemical formula 1] , In the formula (1), R is a linear or branched alkyl group having 1 to 4 carbon atoms.
3. 3. A method for preparing aflatoxin production inhibitor comprises preparing a composition of Klebsiella ) Or Raoultella genus ) And (3) culturing.
4. 4. The method according to claim 3, further comprising a step of purifying a compound represented by the following general formula (1) from the culture obtained in the culturing step, [ Chemical formula 2] , In the formula (1), R is a linear or branched alkyl group having 1 to 4 carbon atoms.
5. 5. A method for inhibiting aflatoxin production, characterized in that the aflatoxin production inhibitor according to claim 1 or 2 is used.
6. 6. A method for controlling aflatoxin contamination, characterized in that the aflatoxin production by aflatoxin-producing bacteria is inhibited by using the aflatoxin production inhibitor according to claim 1 or 2.
7. 7. A compound represented by the following general formula (1), [ Chemical formula 3] , In the formula (1), R is methyl.
8. 8. Klebsiella oxytoca ) The deposit number is NITE BP-03899.

Description

Aflatoxin production inhibitor, aflatoxin production inhibitor produced by aflatoxin production inhibitor, process for producing the same, and method for controlling aflatoxin contamination using the same Technical Field The present invention relates to a novel strain which is an aflatoxin production inhibiting bacterium of a novel microorganism, an aflatoxin production inhibitor produced by the aflatoxin production inhibiting bacterium comprising the novel strain, a process for producing the same, and a method for controlling aflatoxin contamination using the same. Background Among the compounds exhibiting toxicity called mycotoxins, which are secondary metabolites of mold, aflatoxins are the most serious problem. As a microorganism which inhibits the production of aflatoxin, a microorganism which inhibits the production of aflatoxin by inhibiting the growth of aflatoxin-producing bacteria, a microorganism which does not inhibit the growth but can inhibit the production of aflatoxin, a microorganism having the activity of decomposing aflatoxin, a microorganism which adsorbs aflatoxin in a cell, and the like have been found. The bacterial strain has the effect of inhibiting the growth of aflatoxin-producing bacteria and the production of aflatoxin, and is found for bacteria such as bacillus subtilis (Nannocystisexedens), jurass erosion dwarf bacillus (Bacilluspumilus), pseudomonas syringae (Pseudomonassyringae), rare rosington bacteria (Ralstoniapaucula) or Burkholderia (Burkholderiacepacia). And found that Streptococcus lactis (Streptococcuslactis) inhibited aflatoxin production without affecting the growth of aflatoxin-producing bacteria, alcaligenes xylosoxidans (Achromobacterxylosoxidans) inhibited the production of nordisfiguring acid (norsolorinicacid) as a precursor for biosynthesis of aflatoxin. In addition, yeasts of the genera Kluyveromyces (Kluyveromyces), candida (Candida) or Pichia (Pichia) have been reported to inhibit aflatoxin production. The search for aflatoxin-decomposing bacteria has also been conducted, and it has been revealed that aflatoxins are decomposed by, for example, flavobacterium orange (Flavobacteriumaurantiacum), mycobacterium fluoranthene (Mycobacteriumfluoranthenivorans), rhodococcus erythropolis (Rhodococcuserythropolis), and myxococcus orange (Myxococcusfulvus). Lactic acid bacteria such as lactobacillus rhamnosus (Lactobacillusrhamnosus) have been found as bacteria that adsorb aflatoxin to cells (non-patent document 1, above). Further, non-patent document 2 reports that klebsiella (klebsiella sp.) and klebsiella pneumoniae (Klebsiellapneumonia) and the culture supernatant thereof decompose aflatoxins. Some diketopiperazines are known as compounds for inhibiting aflatoxin production, and for example, non-patent document 3 describes a cyclo (L-Leu-L-Pro) produced by alcaligenes xylooxidans (Achromobacter xylosoxidans), and non-patent document 4 describes a cyclo (L-Ala-L-Pro) and a cyclo (L-Val-L-Pro) produced by monospora (stenotomonassp.). Prior art literature Non-patent literature Non-patent document 1, titania, コ, よ, and dye control Mycotoxins,63 (2), 217-224 2013 Non-patent literature 2：Ning, M., Zhang, S., Xie, Y., Wang, W., Gao, Y.: Aflatoxin B1removal by three bacterial strains and optimization of fermentation process parameters. Biotechnology and Applied Biochemistry, 66(6), 930-938(2019) Non-patent literature 3：Yan, P.S., Song, Y., Sakuno, E., Nakajima, H., Nakagawa, H., Yabe, K.: Cyclo (L-Leucyl-L-Prolyl) produced by Achromobacter xylosoxidans inhibits aflatoxin production by Aspergillus parasiticus. Appl Environ Microbiol, 70, 7466-7473(2004) Non-patent literature 4：Jermnak, U., Chinaphuti, A., Poapolathep, A., Kawai, R., Nagasawa, H., Sakuda, S.: Prevention of aflatoxin contamination by a soil bacterium of Stenotrophomonas sp. that produces aflatoxin production inhibitors. Microbiology, 159,902-912(2013) Disclosure of Invention First, the technical problem to be solved The present invention has a technical problem of providing a novel strain which is a novel microorganism having a high activity for inhibiting the production of aflatoxin, an aflatoxin production inhibitor comprising the novel strain, an effective method for producing an aflatoxin production inhibitor comprising the novel strain and an agent having an improved effect, and a method for controlling aflatoxin contamination using the novel strain or the aflatoxin production inhibitor. (II) technical scheme The following invention can solve the above-described problems. [1] An aflatoxin production inhibitor comprising a culture of klebsiella (klebsiella sp.) or ralstonia (raoulella sp.). [2] An aflatoxin production inhibitor comprising a compound represented by the following general formula (1), [ Chemical formula 1] In the formula (1), R is a linear or branched alkyl group having 1 to 4 carbon atoms. [3] A method for producing an aflatoxin production inhibitor, which comprises a step of culturing K