CN-121985887-A - Yeast fermentation broth of salidroside for animal husbandry applications

Abstract

The present disclosure relates to a yeast fermentation broth of salidroside for animal husbandry applications, an animal feed formulation comprising the same, and methods of forming and administering the same.

Inventors

• LIU LU
• Shi Xiabin
• ZHANG DALONG
• WANG SHU
• TIAN JINPING
• SUN CHUNGUANG
• LI HAIPING

Assignees

• 天津利安隆新材料股份有限公司

Dates

Publication Date

20260505

Application Date

20251114

Priority Date

20241210

Claims (20)

1. 1. A feed additive comprising a fermentation broth of a microbial strain.
2. 2. The feed additive of claim 1, wherein the microbial strain is a yeast strain.
3. 3. The feed additive of claim 2, wherein the yeast strain is saccharomyces cerevisiae or a modified variant thereof.
4. 4. The feed additive of claim 1, wherein the feed additive retains at least 95% of its chemical stability after storage at room temperature or about 20 ℃ to 25 ℃ for about six months.
5. 5. The feed additive of claim 1, wherein the fermentation broth comprises salidroside.
6. 6. The feed additive of claim 1, wherein the fermentation broth comprises intact yeast cells, water, a culture medium, and biomolecules secreted by the yeast strain.
7. 7. The feed additive according to claim 1, wherein the fermentation broth is produced by a process comprising activating a yeast strain, seed culturing, and fermenting under controlled conditions of temperature, aeration, pressure, agitation speed, dissolved oxygen, and pH.
8. 8. The feed additive of claim 1, wherein subjecting the fermentation broth to high temperature inactivation conditions comprises maintaining the fermentation broth at a temperature of about 80 ℃ to about 120 ℃ for about 0.5 hours to about 2 hours.
9. 9. An animal feed formulation, the animal feed formulation comprising: A feed additive according to any one of claims 1 to 5, and At least one carrier excipient.
10. 10. An animal feed formulation comprising, calculated based on the total weight of the animal feed formulation, salidroside present in about 10 mg/kg to about 100 mg/kg, and at least one carrier excipient.
11. 11. The animal feed formulation of claim 10, wherein the salidroside is present at about 20 mg/kg to about 60 mg/kg.
12. 12. An animal feed formulation according to claim 10, further comprising at least one additive for animal husbandry selected from the group consisting of astaxanthin, chitosan oligosaccharide, fructooligosaccharides, oligosaccharides, galactooligosaccharides, conjugated linoleic acid, fucoidan, curcumin, bile acids, citric acid ketones, vegetable carbon black, hay heptadecide, berberine, rhodotorula, lutein, beta-carotene, capsanthin, neohesperidin dihydrochalcone, allicin, sodium glutamate, disodium 5 '-inosinate, disodium 5' -guanylate, tea polyphenols and combinations thereof.
13. 13. The animal feed formulation of claim 10, further comprising an acidity regulator selected from the group consisting of formic acid, ammonium formate, calcium formate, acetic acid, sodium diacetate, propionic acid, ammonium propionate, sodium propionate, calcium propionate, butyric acid, sodium butyrate, lactic acid, benzoic acid, sodium benzoate, sorbic acid, sodium sorbate, potassium sorbate, fumaric acid, citric acid, potassium citrate, sodium citrate, calcium citrate, tartaric acid, malic acid, phosphoric acid, sodium hydroxide, sodium bicarbonate, potassium chloride, sodium carbonate, calcium acetate, sodium pyrophosphate, sodium tripolyphosphate, sodium hexametaphosphate, sodium metabisulfite, sodium dihydrogen pyrophosphate, potassium diformate, ammonium chloride, sodium sulfite, and combinations thereof.
14. 14. A method of forming a fermentation broth of a microbial strain, comprising: Activating the microbial strain; Seed culture, and And (5) fermenting and culturing.
15. 15. The method of claim 14, further comprising: subjecting the fermentation broth to high temperature inactivation conditions.
16. 16. The method of claim 15, wherein the high temperature inactivation conditions comprise maintaining the fermentation broth at about 80 ℃ to about 110 ℃ for about 1 hour to about 2 hours.
17. 17. The method of claim 14, wherein the pH of the salidroside fermentation broth is adjusted to a range of about 2.5 to about 3.5.
18. 18. The method of claim 14, wherein the microbial strain is a yeast strain.
19. 19. The method of claim 18, wherein the yeast strain is saccharomyces cerevisiae or a modified variant thereof.
20. 20. The method of claim 14, wherein the fermentation broth comprises salidroside.

Description

Yeast fermentation broth of salidroside for animal husbandry applications The present application claims priority from U.S. provisional application Ser. No. 63/730,188 filed on 10/12/2024, the disclosure of which is incorporated herein by reference. Technical Field The present disclosure relates to animal husbandry applications, and in particular, to additives for use in animal feed formulations. Background During the growth and development of animals, energy, protein, amino acids, minerals and trace element nutrients are required. When the common feed can not meet the growth requirement of animals, the feed additive containing trace elements or other nutrient substances can promote the animals to grow rapidly. In addition, the additive can also improve the palatability of the feed, promote animal development, treat animal diseases and reduce production cost. The non-nutritional feed additive comprises microecological preparation, enzyme preparation, traditional Chinese medicine preparation and its extract. The plant extract has the ability to improve animal immune function and environmental stress response. However, from a process point of view, the components of the plant extract are relatively complex and difficult to produce due to the high extraction costs. Some components are difficult to obtain pure products, making large-scale industrial production impossible. From the perspective of product standards, the active ingredient content of medicinal materials of different varieties and different producing areas is different, and the extraction quality difference is large. Object and content of the invention Certain embodiments of the present invention relate to a feed additive comprising a fermentation broth of a microorganism strain. In certain embodiments, the microbial strain is a yeast strain. In certain embodiments, the yeast strain is Saccharomyces cerevisiae or a modified variant thereof. In certain embodiments, the feed additive retains at least 95% of its chemical stability after storage at room temperature or about 20 ℃ to 25 ℃ for about six months. In certain embodiments, the fermentation broth comprises salidroside. Certain embodiments of the present invention relate to an animal feed formulation comprising a feed additive as disclosed herein, and at least one carrier excipient. Certain embodiments of the present invention relate to an animal feed formulation comprising salidroside, e.g., present in about 1 mg/kg to about 150 mg/kg, about 1 mg/kg to about 100 mg/kg, about 10 mg/kg to about 100 mg/kg, or about 10 mg/kg to about 20 mg/kg, about 20 mg/kg to about 60 mg/kg, or about 60 mg/kg to about 80 mg/kg, calculated based on the total weight of the animal feed, and at least one carrier excipient. Carrier excipients may include solvents (e.g., water), preservatives, mold inhibitors, and acidity regulators. The acidity regulator may be selected from the group consisting of formic acid, ammonium formate, calcium formate, acetic acid, sodium diacetate, propionic acid, ammonium propionate, sodium propionate, calcium propionate, butyric acid, sodium butyrate, lactic acid, benzoic acid, sodium benzoate, sorbic acid, sodium sorbate, potassium sorbate, fumaric acid, citric acid, potassium citrate, sodium citrate, calcium citrate, tartaric acid, malic acid, phosphoric acid, sodium hydroxide, sodium bicarbonate, potassium chloride, sodium carbonate, calcium acetate, sodium pyrophosphate, sodium tripolyphosphate, sodium hexametaphosphate, sodium metabisulfite, sodium dihydrogen pyrophosphate, potassium diformate, ammonium chloride, and sodium sulfite. The animal feed formulation may further comprise at least one additive for animal husbandry. The animal feed formulation may comprise astaxanthin, chitosan oligosaccharide, fructooligosaccharide, oligosaccharide, galactooligosaccharide, conjugated linoleic acid, fucoidan, curcumin, bile acid, citric acid ketone, plant soot, hay heptadectin, berberine, rhodotorula, lutein, beta-carotene, capsanthin, neohesperidin dihydrochalcone, allicin, sodium glutamate, disodium 5 '-inosinate, disodium 5' -guanylate, tea polyphenols. Certain embodiments of the invention relate to a method of forming a fermentation broth of a microbial strain, the method comprising activating the strain, seed culture, and fermentation culture. Certain embodiments of the method further comprise subjecting the fermentation broth to high temperature inactivation conditions. In certain embodiments of the method, the high temperature inactivation conditions comprise maintaining the fermentation broth at about 80 ℃ to about 110 ℃ for about 0.5 hours to about 2 hours. In certain embodiments of the method, the microbial strain may be a yeast strain, such as Saccharomyces cerevisiae or modified variants thereof. Certain embodiments of the invention relate to a method of feeding an animal comprising administering to the animal a feed additive or feed formulation as disclosed herein. In certain embo