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CN-121985937-A - Novel compositions for anti-ageing treatment of skin

CN121985937ACN 121985937 ACN121985937 ACN 121985937ACN-121985937-A

Abstract

The present invention relates to the treatment and/or prevention of skin aging. More particularly, the present invention relates to the application of living microorganisms to improve the appearance of skin and reduce skin aging phenomena.

Inventors

  • Helena Falholt Elvibak
  • Ida Blomquest Christensen
  • Serena karrouf
  • Charlotte Wezel

Assignees

  • 欧莱雅

Dates

Publication Date
20260505
Application Date
20240806
Priority Date
20230807

Claims (20)

  1. 1. A composition for use in treating, alleviating, inhibiting, preventing and/or preventing skin aging, wherein the composition comprises at least one viable isolated microorganism.
  2. 2. Composition for use in the treatment, alleviation, inhibition, prevention and/or prevention of skin ageing of claim 1, wherein the composition comprises at least one viable isolated microorganism providing: (a) Reducing wrinkles by at least 5 percent; (b) Reducing the pigment spot density by at least 5 percent, and/or (C) The degree of compaction measured as a reduction in suction distance by more than 5 percent is increased.
  3. 3. The composition for use in treating, alleviating, inhibiting, preventing and/or preventing skin aging according to claim 1, wherein said composition comprises at least 2 live lactic acid bacteria for topical treatment of the skin, wherein after a topical treatment of 28 days per day an increase in dermal density of at least 5 percent is obtained.
  4. 4. Composition for use in the treatment, alleviation, inhibition, prevention and/or prevention of skin ageing of claim 1, wherein the composition comprises at least one viable isolated microorganism providing: (a) Reducing the subsurface hypoechoic area (SLEB) thickness by at least 2 percent, and/or (B) Reducing TEWL by at least 2 percent.
  5. 5. Composition for use in the treatment, alleviation, inhibition, prevention and/or prevention of skin ageing of claim 1, wherein the composition comprises at least one viable isolated microorganism providing: (a) Increasing skin tone glossiness by more than 10 percent, and/or (B) Increasing the smoothness by more than 10 percent.
  6. 6. A composition according to any of the preceding claims, wherein the microorganism is a lactic acid bacteria.
  7. 7. The composition of claim 6, wherein the lactic acid bacteria produce antimicrobial metabolites and/or bacteriocins.
  8. 8. The composition of any one of claims 1 to 7, wherein the change from baseline is observed within 20 days, such as within 28 days, for example within 35 days, for example within 46 days, such as within 50 days, for example within 56 days, from the application of the first dose of the composition.
  9. 9. The composition according to any of the preceding claims, wherein the composition is a topical composition.
  10. 10. The composition of any of the preceding claims, wherein the topical composition is a cream, lotion, spray, solution, gel, serum, ointment, fat, oil, powder, paste, foam, paste, paint, adhesive, suspension, or emulsion.
  11. 11. A composition according to any preceding claim, wherein the composition, for example the topical composition, comprises viable microorganisms at a concentration of at least 10 4 CFU/g, such as at least 10 5 CFU/g, such as at least 10 6 CFU/g, such as at least 10 7 CFU/g, such as at least 10 8 CFU/g.
  12. 12. The composition of any of the preceding claims, wherein the composition further comprises at least one natural lipid.
  13. 13. A method of skin treatment comprising applying a live topical composition comprising at least one isolated lactic acid bacteria for at least 28 days for treating, alleviating, inhibiting, preventing and/or preventing skin aging.
  14. 14. The skin treatment method according to claim 13, comprising administering a live topical composition comprising at least one isolated lactic acid bacteria for at least 28 days for treating, alleviating, inhibiting, preventing and/or preventing skin aging, wherein the method provides: (a) Reducing wrinkles by at least 5 percent; (b) Reducing the pigment spot density by at least 5 percent, and/or (C) The degree of compaction measured as a reduction in suction distance by more than 5 percent is increased.
  15. 15. The skin treatment method according to claim 13, comprising administering a live topical composition comprising at least one isolated lactic acid bacteria for at least 28 days for treating, alleviating, inhibiting, preventing and/or preventing skin aging, wherein the method provides: (a) Increasing the dermis density by at least 5 percent; (b) Increase skin hydration by more than 10 percent, and/or (C) Increasing skin elasticity by more than 2 percent.
  16. 16. The skin treatment method according to claim 13, comprising administering a live topical composition comprising at least one isolated lactic acid bacteria for at least 28 days for treating, alleviating, inhibiting, preventing and/or preventing skin aging, wherein the method provides: (a) Reducing the subsurface hypoechoic area (SLEB) thickness by at least 2 percent, and/or (B) Reducing TEWL by at least 2 percent.
  17. 17. The skin treatment method according to claim 13, comprising administering a live topical composition comprising at least one isolated lactic acid bacteria for at least 28 days for treating, alleviating, inhibiting, preventing and/or preventing skin aging, wherein the method provides: (a) Increasing skin tone glossiness by more than 10 percent, and/or (B) Increasing the smoothness by more than 10 percent.
  18. 18. The method according to claims 13 to 17, wherein a concentration of at least 10 4 CFU/cm 2 , such as at least 10 5 CFU/cm 2 , such as at least 10 6 CFU/cm 2 , such as at least 10 7 CFU/cm 2 , such as at least 10 8 CFU/cm 2 , of viable microorganisms is applied to the skin.
  19. 19. The method of any one of claims 13 to 18, wherein the viable microorganisms are applied to the skin at least once daily.
  20. 20. The method of any one of claims 13 to 19, wherein the living microorganism survives on the skin for at least 8 hours after topical application.

Description

Novel compositions for anti-ageing treatment of skin Technical Field The present invention relates to compositions and methods for treating skin aging, and in particular to topical compositions and methods of use. The present invention relates to topical compositions comprising viable microorganisms. In particular, the present invention relates to compositions and methods of use of topical compositions comprising viable probiotic microorganisms for improving the appearance of skin and reducing skin aging phenomena. In particular, the present invention relates to novel compositions comprising living microorganisms having active metabolism on the skin. Background The present invention relates to compositions, uses of the compositions and methods for preventing or treating skin aging or photodamage. The composition comprises viable probiotic microorganisms capable of reversing the signs of skin aging. During aging, the skin can undergo significant clinical and morphological changes. Physiological aging of the skin occurs due to intrinsic and extrinsic factors, known as chronological aging and photoaging, respectively. Intrinsic factors are driven by telomere shortening and cellular senescence, while extrinsic factors such as ultraviolet light and pollution lead to Reactive Oxygen Species (ROS) and free radicals. Aging, a process that gradually declines with age, affects everyone. One of the most visible visual consequences of aging can be seen on the skin. The skin loses its firmness and elasticity, resulting in sagging, more pronounced pores and the appearance of wrinkles. In addition, the turnover rate of skin cells decreases, the skin looks less shiny, the appearance is rougher, and the number of pigment spots increases as the sebaceous gland cell activity decreases and becomes drier. Aging of the skin may be considered at several levels, such as phenotypic (as described above), histological, and/or molecular levels. Histologically, the junction between dermis and epidermis flattens and melanocyte density decreases. The dermis thickness decreases with both reduced vascularization and the number of mast cells and fibroblasts. The reduced skin elasticity and firmness are the result of changes in the extracellular matrix (ECM) of the dermis, such as changes in the production and location of collagen fibers and elastic fibers. Gland activity is also reduced, resulting in reduced sebum and thus drier skin. At the cellular level, aging is due to the accumulation of aging cells, changes in cell cycle, and the accumulation of free radical/oxidative stress and changes in the glycation pattern (particularly of collagen). Signs of aging, such as dryness, roughness, pigment spots and wrinkles, as well as decreased skin barrier and altered biomechanical function, are major histological changes. As the aging of the western population increases, treatments capable of preventing and improving signs of skin aging are receiving increased attention for their cosmetic and economic potential. The above-mentioned age-related skin changes occur due to intrinsic factors including time-series factors and genetic factors and extrinsic factors due to environmental influences such as sun exposure (called photoaging), pollution exposure and smoking. In particular, sun exposure can lead to the generation of Reactive Oxygen Species (ROS) and free radicals, ultimately leading to oxidative stress and subsequent cell damage. Furthermore, recent studies by Howard et al (2022) have found that with age, the diversity of the skin microbiome increases at all sampling sites, but the abundance of Lactobacillus decreases, indicating that the skin microbiome may also play a role in skin aging. Aging occurs due to a number of factors that interact and interact intricately. These changes can not only disqualify some individuals, but can also cause problems with the protective barrier and subsequent medical problems. As the population ages, particularly in the western world, there is a great cosmetic and economic interest in reversing/preventing the appearance of aging signs. As the aging of the western population increases, treatments capable of preventing and improving signs of skin aging are receiving increased attention for their cosmetic and economic potential. The anti-aging efficacy of facial skin can be assessed by assessing subsurface hypoechoic band (SLEB) thickness, dermis density, skin firmness and elasticity, skin hydration, TEWL, fish tail, plaque, smoothness and skin tone gloss and comparing to baseline measurements. SLEB has been shown previously to quantify skin aging, the thickness of which reflects the extent of skin aging (GNIADECKA et al 1994). Therefore, SLEB has been shown to be significant in quantifying aging, especially in light-exposed areas, such as the cheekbone area. The association of skin lipids/bacteria may be important for age-dependent changes in the skin microbiota. In the present invention, active lactic acid bacter