CN-121986623-A - Method for improving salt tolerance of wheat seeds

Abstract

The invention discloses a method for improving salt tolerance of wheat seeds, which belongs to the technical field of salt tolerance regulation of the wheat seeds and comprises the following steps of randomly selecting full and mildew-free wheat seeds to be sown, sterilizing by using 0.1% of raw mercury for 2min, washing 5 times by using clear water, germinating the seeds in the clear water for 2h, taking out the seeds, washing 3 times by using the clear water, soaking the seeds in 0.3% of sodium chloride solution for 3h, soaking the seeds in the clear water for 2h, soaking the seeds in 0.6% of sodium chloride solution for 4h, soaking the seeds in the clear water for 2h and soaking the seeds in 0.9% of sodium chloride solution for 5h, and subjecting the wheat seeds obtained through stress treatment of progressive sodium chloride solution to eliminate sensitivity to salt stress and excite adaptability to salt stress. According to the invention, the sensitivity of the seeds to salt stress is eliminated by setting different concentrations of sodium chloride stress environments, so that the salt tolerance of the wheat seeds is improved.

Inventors

• XIAO LEI
• YANG PU
• ZHU CHUNLEI
• LU QIXIANG
• LEI CHAO
• CHEN WEINAN

Assignees

• 邯郸市农业科学院

Dates

Publication Date

20260508

Application Date

20260210

Claims (5)

1. 1. A method for improving salt tolerance of wheat seeds, comprising the steps of: S1, randomly selecting full, mildew-free and active wheat seeds to be sown in saline-alkali soil, sterilizing for 2min by using 0.1% of raw mercury, and washing for 5 times by using clear water to remove the raw mercury attached to the surfaces of the seeds; S2, placing the seeds in the step S1 in a mesh bag, and then placing the seeds together with the mesh bag in clear water for germination accelerating treatment for 2 hours; S3, enabling the seeds in the step S2 to leave the water surface together with the mesh bag, repeatedly flushing the seeds with clear water for 3 times, and repeatedly shaking the mesh bag to drain water for later use; s4, soaking the seeds in the S3 in 0.3% sodium chloride solution for 3 hours; S5, enabling the seeds in the step S4 to leave the water surface together with the mesh bag, flushing the seeds with clear water for 5 times, and repeatedly shaking the mesh bag to drain water for later use; s6, soaking the seeds in the step S5 in clear water for 2 hours; s7, enabling the seeds in the step S6 to leave the water surface together with the mesh bag, flushing the seeds with clear water for 3 times, and repeatedly shaking the mesh bag to drain water for later use; S8, placing the seeds in the S7 together with the mesh bag into a 0.6% sodium chloride solution for soaking for 4 hours; s9, separating the seeds in the step S8 from the water surface together with the mesh bag, cleaning the seeds with clear water for 5 times, and repeatedly shaking the mesh bag to drain water for later use; s10, soaking the seeds in the step S9 in clear water for 2 hours; s11, enabling the seeds in the step S10 to leave the water surface together with the mesh bag, repeatedly flushing the seeds with clear water for 3 times, and repeatedly shaking the mesh bag to drain water for later use; S12, soaking seeds in the S11 in 0.9% sodium chloride solution for 5 hours; s13, enabling the seeds in the S12 to leave the water surface together with the mesh bag, flushing the seeds with clear water for 5 times, repeatedly shaking the mesh bag, and airing the seeds under the condition of ventilation and light transmission for later use; And S14, carrying out progressive sodium chloride stress treatment to obtain the wheat seeds in S13, wherein the sensitivity to sodium chloride stress is eliminated, and the adaptability to salt stress is stimulated.
2. 2. The method for improving salt tolerance of wheat seeds according to claim 1, wherein the mesh bag used in S2-S13 has a smaller pore size than wheat grains, ensuring that wheat grains cannot be leaked out.
3. 3. A method for improving salt tolerance of wheat seeds according to claim 1, wherein the seeds in S2, S6 and S10 should be submerged in clear water but the water surface should not be higher than the uppermost part of the seeds in the upper layer.
4. 4. A method of improving salt tolerance of wheat seeds according to claim 1, wherein the seeds in S4, S8 and S12 are immersed in a sodium chloride solution but the solution level is not higher than the uppermost part of the seeds in the upper layer.
5. 5. The method for improving salt tolerance of wheat seeds according to claim 1, wherein the concentration of sodium chloride in S4, S8 and S12 is mass fraction.

Description

Method for improving salt tolerance of wheat seeds Technical Field The invention relates to the technical field of wheat seed salt tolerance regulation and control, in particular to a method for improving wheat seed salt tolerance. Background Wheat is a main grain crop in China and is also an important strategic material. Under the basic national conditions of large population base and relatively small cultivated land area, the excavation of usable cultivated land has important significance for guaranteeing the safe production of wheat. At present, the area of the saline-alkali soil in China reaches 2.99 hundred million mu, wherein the available saline-alkali soil area is about 5460 ten thousand mu, and the saline-alkali soil becomes an important soil backup resource at present. However, soil salinization can cause soil hardening and fertility reduction, cause unbalance of soil nutrients and increase of osmotic pressure, inhibit germination of wheat seeds and growth of seedlings, severely restrict growth and development of wheat, cause yield reduction and even harvest failure of wheat, and threaten grain safety. In recent years, the soil salinization degree is further increased due to the reduction of rainfall and the unreasonable chemical fertilizer application and irrigation modes in production. Therefore, the salinization area of the soil in China has an increasing trend year by year. With the gradual increase of the area of the salinized soil, the available cultivated area in China has a gradual decrease trend, and the sustainable development of agriculture is restricted. In order to effectively develop and utilize the existing land, the potential of the saline-alkali land needs to be mined through technological innovation, and the strategic aim of storing grains in the land and storing grains in the skill is achieved. The saline-alkali soil is mainly composed of salinized soil and alkalized soil, wherein the salinized soil is the largest in proportion of sodium chloride. The sodium chloride exceeding in the soil brings a series of adverse effects on wheat production, namely, an osmotic stress environment is formed, the osmotic pressure of a soil solution is increased, wheat root systems are difficult to absorb water, physiological drought occurs, seedling stage is easy to wilt, seedling emergence rate is low, ionic toxicity is generated, excessive Na +、Cl- accumulation is caused, the cell membrane structure of the wheat is damaged, enzyme activity is inhibited, photosynthesis and respiration are influenced, leaf scorch and growth arrest are caused, wheat nutrition imbalance is caused, na +, ca 2+、K+ and other competitive root system absorption sites are caused, wheat nutrient deficiency (such as low potassium and calcium deficiency) is caused, meanwhile, the high pH value can reduce the validity of microelements such as P, fe and Zn, yellowing and premature senility are caused, the physical and chemical property of the soil is deteriorated, the viscosity and the hardening of the alkalinized soil are caused, the ventilation and water permeability are poor, root systems are difficult to take root systems, the microbial activity is reduced, the nutrient conversion is hindered, physiological metabolism of the wheat is caused to be disturbed, active oxygen in the wheat is caused, an antioxidant system is unbalanced, the grains are easy to be shrunken in the accumulation stage, and the thousand grain weight is easy to reduce, and finally, and even the wheat is recovered. Thus, adaptation of wheat to a sodium chloride stress environment represents adaptation to salinized soil. The improvement of the sodium chloride stress resistance of the wheat has practical significance for improving the adaptation of the wheat to the saline-alkali soil environment. At present, the prior art improves the salt tolerance of wheat seeds through exogenous signal molecules, hormones, trace elements and novel nano materials, and the use of the substances has relatively high cost and insignificant effect, and can influence other physiological metabolism activities of wheat to adversely affect the growth and development of the wheat. In order to solve the problems, the invention provides a method for regulating and controlling the salt tolerance of wheat seeds, which eliminates the sensitivity of the seeds to sodium chloride stress by setting different concentration sodium chloride stress environments, thereby improving the salt tolerance of the wheat seeds. Aiming at the potential capability of the wheat to adapt to survival, the invention provides a method for regulating and controlling the salt tolerance of the wheat seeds, which aims to overcome the defects of the prior art, skillfully excites the adaptability of the salt tolerance of the wheat, sets different concentration sodium chloride stress environments indoors, gradually eliminates the sensitivity of the wheat seeds to salt by utilizing different concentration sodium chl