CN-121986627-A - Chemical method for removing tomato brown wrinkle virus in tomato seeds

Abstract

The invention discloses a chemical method for removing tomato brown wrinkled fruit virus in tomato seeds, which comprises the steps of preparing mixed solution, treating with chemical agent, filtering seeds, rinsing and airing. The invention realizes a new method for completely removing the brown tomato crinkle fruit virus on tomato seeds by optimizing the disinfection effect of the conventional disinfectant, and solves the contradiction of detoxification rate, survival rate, seed vitality and propagation of seed coat virus to the buds. The seeds are treated for 15min by a solution containing Tween 20 with the final mass percentage concentration of 0.1% and sodium hypochlorite with the final mass percentage concentration of 2.5%, the germination potential of the seeds is 54.7%, the germination rate is 96.7%, the detoxification rate is 100%, and the toxicity rate of the sprouts is 0%. Therefore, the detoxification method can effectively remove the tomato brown wrinkled fruit virus on the tomato seeds, and has no influence on the activity and germination rate of the seeds.

Inventors

• SHANG QIAOXIA
• HONG RUIJIE
• CHEN YONGPAN
• LIU YIRAN
• FENG LINLIN
• YU CUI

Assignees

• 北京农学院

Dates

Publication Date

20260508

Application Date

20260125

Claims (3)

1. 1. A chemical method for removing tomato brown wrinkle virus in tomato seeds is characterized by comprising the following steps: S1, preparing chemical agents, namely mixing tween 20 and sodium hypochlorite solution to prepare a solution containing tween 20 with the final mass percentage concentration of 0.1% and sodium hypochlorite with the final mass percentage concentration of 2.5%; S2, treating and filtering the tomato seeds by using a chemical agent, namely soaking the tomato seeds by using a solution containing tween 20 with the final mass percentage concentration of 0.1% and sodium hypochlorite with the final mass percentage concentration of 2.5%, and filtering to obtain the tomato seeds; S3, rinsing, namely flushing tomato seeds with water for 1h, and filtering to dry the water; S4, airing, namely placing the tomato seeds obtained in the step S3 into a culture dish, and naturally airing the tomato seeds for 72 h to prevent the seeds from sprouting and mildew.
2. 2. Chemical process according to claim 1, characterized in that in step S2 the volume ratio of tomato seed to solution containing tween 20 at a final mass percentage concentration of 0.1% and sodium hypochlorite at a final mass percentage concentration of 2.5% is 1:5.
3. 3. A chemical process according to claim 1, characterized in that in step S2, the soaking time is 15 minutes.

Description

Chemical method for removing tomato brown wrinkle virus in tomato seeds Technical Field The invention relates to the technical field of seed treatment, in particular to a chemical method for removing tomato brown wrinkle fruit virus in tomato seeds. Background Tomatoes are global important economic crops, occupy a core position in the vegetable industry in China, and the yield and the quality of the tomatoes are directly related to economic benefits of growers and stable in market supply. Tomato brown wrinkle virus (ToBRFV) has become a critical disease threatening the tomato industry as a highly pathogenic virus that spreads rapidly in recent years. In order to prevent and control the harm and transmission of the virus, the common tomato seed detoxification treatment technology at present mainly comprises dry heat treatment, chemical agent treatment, hydrochloric acid treatment, trisodium phosphate treatment, sodium hypochlorite treatment and the like, but the low-concentration short-time treatment can not thoroughly remove the virus in the seed by 100 percent, when a sodium hypochlorite solution is used as a disinfectant, the concentration is increased and the treatment time is prolonged, the virus can be completely removed, but the germination vigor and the germination rate of the tomato seed can be obviously influenced, the tomato seed can not be used as seeds any more, the economic loss is caused, the virus is caused to have transmission risk, therefore, the activity of the virus is passivated, the seed is kept high in activity, the normal use in the agricultural production is ensured, the risk is reduced, and the production safety is ensured. Tween 20 is a widely used nonionic surfactant, the surface of the seed is often provided with a waxy layer or a hydrophobic group, a pure water-based sodium hypochlorite solution is difficult to spread completely and is easy to form water drop loss, the hydrophilic end of the Tween 20 faces to the water phase and the hydrophobic end and is adsorbed on the surface of the seed, the surface tension of a solid-liquid interface can be obviously reduced, a disinfection solution can uniformly cover concave-convex gaps of the seed, the disinfection effect is improved, but the high concentration Tween 20 can reduce the activity and germination rate of the seed, so that a low concentration Tween and sodium hypochlorite mixed solution is selected as a chemical agent for removing ToBRFV. Seed detoxification effects can be detected by a variety of methods. Such as post-emergence symptom observation, enzyme-linked immunosorbent assay, RT-PCR method, etc. Because of the difference of virus symptoms and the existence of potential recession, the activity of the virus carried by the seeds is difficult to judge by adopting an enzyme-linked immunosorbent assay and an RT-PCR method. Therefore, it is difficult to judge whether the seed detoxification effect affects the seed quality. The research and development adopts PCR detection to the seeds after detoxification and the buds after budding, not only can detect the influence on the quality of the seeds after the detoxification treatment of the seeds, but also can effectively detect the detoxification effect at the same time. Disclosure of Invention The invention aims to overcome the defects of the prior art, optimize the conventional common disinfectant and provide a chemical method for removing the tomato brown wrinkle virus in tomato seeds by adding a surfactant into the common disinfectant, so that ToBRFV in the tomato seeds can be thoroughly removed, and the activity and germination rate of the tomato seeds can not be reduced. In order to achieve the aim, the technical scheme adopted by the invention is as follows, and the chemical method for removing the tomato brown wrinkle virus in the tomato seeds comprises the following steps: S1, preparing chemical agents, namely mixing tween 20 and sodium hypochlorite solution to prepare a solution containing tween 20 with the final mass percentage concentration of 0.1% and sodium hypochlorite with the final mass percentage concentration of 2.5%; s2, treating and filtering the tomato seeds by using a chemical agent, namely soaking the tomato seeds with 5 times of a solution containing tween with the final mass percentage concentration of 0.1% and sodium hypochlorite with the final mass percentage concentration of 2.5%, and filtering to obtain the tomato seeds; S3, rinsing, namely flushing tomato seeds with water for 1h, and filtering to dry the water; s4, airing, namely placing the tomato seeds obtained in the step S3 into a culture dish, naturally airing 72 h, and preventing the seeds from being planted Seed germination and mildew. The invention not only can not influence the germination vigor and germination rate of tomato seeds, but also can remove ToBRFV of seeds more thoroughly, and the detoxification rate can reach 100%. The present invention will be described in detail with referenc