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CN-121986684-A - Mechanism research for regulating and controlling anthocyanin synthesis of waxberry fruits by optical signals and application of mechanism research

CN121986684ACN 121986684 ACN121986684 ACN 121986684ACN-121986684-A

Abstract

The invention provides a mechanism study for regulating and controlling synthesis of anthocyanin in waxberry fruits by using a light signal and application thereof. The quality characteristics of mature fruits are analyzed by adopting different light-transmitting bags for the Myrica rubra Dongkui, and the comprehensive analysis is combined with transcriptome and metabolome. The result shows that the bagging in the young fruit stage obviously reduces the quality of fruits, and the black opaque bag inhibits the color transfer and anthocyanin synthesis of the fruits, so that the influence of the bagging in the color transfer stage is small. Metabolome and transcriptome analysis showed that differential metabolites are enriched in anthocyanin synthesis and flavonoid metabolic pathways, related differential metabolites were identified, and core regulatory transcription factors HY5 and DF1 were involved in the light regulated anthocyanin accumulation process. The invention defines the optical signal regulation and control mechanism, identifies related factors and metabolites, and provides basis for the quality improvement and cultivation technology optimization of the waxberries.

Inventors

  • ZHANG SHUWEN
  • QI XINGJIANG
  • YANG HAN
  • Yu Zheping
  • SUN LI
  • ZHANG YANG
  • YING ZHENGZHENG

Assignees

  • 浙江省农业科学院

Dates

Publication Date
20260508
Application Date
20241108

Claims (10)

  1. 1. A method for regulating and controlling anthocyanin content in waxberry fruits is characterized in that the anthocyanin content in the waxberry fruits is regulated and controlled by regulating expression of transcription factor HY5 gene or regulating activity of transcription factor HY5 protein, the nucleotide sequence of the HY5 gene is shown as SEQ ID NO.1, and the amino acid sequence of the HY5 protein is shown as SEQ ID NO. 2.
  2. 2. The method of claim 1, wherein the anthocyanin content in the waxberry fruits is regulated by regulating the intensity of the optical signal, regulating the expression of the transcription factor HY5 gene or regulating the activity of the transcription factor HY5 protein.
  3. 3. The method of claim 2, wherein the anthocyanin content in the waxberry fruits is increased by increasing the intensity of the optical signal, increasing the expression of the transcription factor HY5 gene or increasing the activity of the transcription factor HY5 protein.
  4. 4. The method of claim 1, wherein the anthocyanin content in the waxberry fruits is regulated by regulating the transcription factor HY5 related gene, regulating the expression of the transcription factor HY5 gene or regulating the activity of the transcription factor HY5 protein through genetic engineering means.
  5. 5. The method of claim 1, wherein the anthocyanin content in the waxberry fruits can be regulated by regulating the expression of a transcription factor DF1 gene or regulating the activity of a transcription factor DF1 protein, wherein the nucleotide sequence of the DF1 gene is shown as SEQ ID NO.3, and the amino acid sequence of the DF1 protein is shown as SEQ ID NO. 4.
  6. 6. The method of claim 5, wherein the anthocyanin content in the waxberry fruits is regulated by regulating the intensity of the optical signal, regulating the expression of the transcription factor DF1 gene or regulating the activity of the transcription factor DF1 protein.
  7. 7. The application of the transcription factor HY5 in preparing a preparation for improving the anthocyanin synthesis of the waxberry fruits is characterized in that the transcription factor HY5 is used as an active ingredient of the preparation to participate in regulating and controlling the anthocyanin synthesis process of the waxberry fruits, and is positively correlated with the anthocyanin content and the anthocyanin biosynthesis gene expression.
  8. 8. The use according to claim 7, wherein in the waxberry fruits, anthocyanin synthesis genes comprise any one or more of chalcone synthase genes, flavonol synthase genes and colorless anthocyanin dioxygenase genes, and transcription factor HY5 is positively correlated with the expression of the genes.
  9. 9. The use according to claim 7, wherein the preparation affects the activity of transcription factor HY5 by modulating the expression of a light signal transduction pathway-related gene in waxberry fruits, thereby modulating anthocyanin synthesis.
  10. 10. The use according to claim 9, wherein the related genes of the optical signal transduction pathway in the waxberry fruits comprise any one or more of a light receptor gene and a COP1 gene, and the transcription factor HY5 is used as a downstream receptor of the related genes of the optical signal transduction pathway, and further regulates anthocyanin synthesis through interaction with the COP1 gene and regulation of anthocyanin synthesis genes.

Description

Mechanism research for regulating and controlling anthocyanin synthesis of waxberry fruits by optical signals and application of mechanism research Technical Field The invention belongs to the technical field of plant cultivation and fruit quality regulation, and particularly relates to a mechanism study for regulating synthesis of anthocyanin in waxberry fruits by using a light signal and application thereof. Background The waxberry is a fruit with extremely high economic value and nutritive value, and the enriched anthocyanin has the characteristic of promoting health. Illumination is one of the most important environmental factors influencing fruit color, and anthocyanin synthesis process is obviously regulated and controlled by illumination in the maturation process of the waxberry fruits. Chinese bayberry (Myrica rubra cv. Dongkui Orient Pearl) is a Yang Meishu subtropical evergreen tree of Myricaceae, mainly distributed in south China and other Asian countries, and the fruits are touted by people due to their unique flavor and nutritional value, and have extremely high economic value (Zhang et al, 2024). The plant extract is rich in anthocyanin and other flavonoid substances, is used as an important secondary metabolite in the plant, has an adjusting effect on physiological functions of the plant, such as ultraviolet resistance, high temperature, drought resistance and the like, provides abundant nutrition and medicinal resources for human beings, and can help people to prevent various diseases (Tsuda et al 2012). Along with the increasing demands of consumers on health foods, the research on anthocyanin in waxberry fruits is particularly important. The color change of the waxberry fruits is mainly caused by the change of anthocyanin content, is the most visual mark in the maturation process, and is followed by a complex pigment metabolism path and a fine regulation network. Previous studies have shown that the anthocyanin biosynthesis process is progressively catalyzed by chalcone synthase (CHS), chalcone isomerase (CHI), flavone-3-hydroxylase (F3H), flavonol 4-reductase (DFR) and anthocyanin synthase (ANS) and regulated by a number of transcription factors (Tsuda et al 2012). R2R3-MYB, bHLH and WDR transcription factors regulate anthocyanin synthesis through a variety of pathways, wherein genes such as MYB1, MYB2, MYB10, MYB90, MYB113 and the like all play a central role in anthocyanin biosynthesis (Cui et al, 2023; lim et al, 2016; zhang et al, 2024; sun et al, 2021; liu et al, 2024). Optical signals play a vital role in plant growth and development, especially during fruit ripening. Previous studies have shown that anthocyanin biosynthesis in eggplant peel is totally dependent on light, anthocyanin accumulation does not occur in eggplant peel under dark conditions, and that interaction of blue light receptor CRY1/CRY2 with COP1 is found to regulate binding of MYB1 transcription factor to downstream anthocyanin synthesis genes (CHS and DFR), thereby affecting anthocyanin synthesis pathway in fruits (Jiang et al 2016). The light signal factor BoMYB R1 directly inhibited the promoter activity of the BoMYB b gene in collard, and the expression level was significantly down-regulated by strong light signals (Liu et al, 2024). In reports on anthocyanin synthesis in tea trees, csbHLH can bind to the G-box element of the promoters of CsCHS, csFLS and CsDFR, positively regulate anthocyanin synthesis in tea trees, and the light signal transduction factor CsHY can bind to the promoter of CsbHLH89, indirectly promoting anthocyanin accumulation (Zhang et al, 2023). Therefore, the light signal activates a series of signal transduction pathways through the action of various light receptors, and further influences the biosynthesis and distribution of pigments, and although the light signal is studied on the regulation and control of the synthesis of other plant anthocyanin, the direct substance accumulation and regulation mechanisms of various species have obvious differences at present. In the waxberry fruits, the anthocyanin accumulation way is not clear, and meanwhile, the mechanism of regulating anthocyanin synthesis by a light signal is not clear. Therefore, a mechanism for regulating and controlling anthocyanin synthesis of the waxberry fruits by optical signals is urgently needed, the influence of bagging and optical treatment on the pigmentation of the waxberry fruits is discussed, the effects of the bagging and optical treatment on the fruit ripening, the color formation and the quality improvement are analyzed, the synthesis path of the anthocyanin in the waxberry fruits is finely regulated and controlled by utilizing the combined analysis of transcriptome and metabonomics to excavate the optical signals, the action mechanism of the anthocyanin in the fruit ripening is revealed, and a solid scientific basis is provided for the improvement of the quality of the waxberry fruits and the optimization of faci