CN-121986688-A - Method for promoting purple perilla quality improvement based on microbial composition

Abstract

The invention discloses a method for promoting purple perilla quality improvement based on a microorganism composition, and belongs to the technical field of plant cultivation. The invention provides a cultivation method of purple perilla, which comprises the steps of sowing a arbuscular mycorrhizal fungus agent containing rhizopus radiatus in a sowing period of purple perilla after ditch application, and respectively carrying out root irrigation treatment by using a composite fungus agent containing bacillus amyloliquefaciens and pseudomonas fluorescens in a seedling period and a current sequence period of purple perilla, thereby realizing the cooperative regulation and control of growth and development of purple perilla and metabolic paths. The method not only effectively solves the problems of single function, extensive application and unstable effect of microorganisms in the prior art, but also accurately promotes the efficient conversion of photosynthetic products to grain oil and fat through the intervention of stages and multiple targets, and specifically enhances the biosynthesis path of alpha-linolenic acid. The invention provides an effective new scheme for the high-yield high-quality green production of the purple perilla, and has important application value and popularization prospect.

Inventors

• CHEN SI
• CHEN JING
• YIN WEIPING
• LIU YAN
• LI CEN
• MA JUN
• BIAN DAOLIN
• WU LINLIN

Assignees

• 黑龙江省农业科学院经济作物研究所

Dates

Publication Date

20260508

Application Date

20260211

Claims (10)

1. 1. A method for improving the quality of perilla based on a microbial composition, comprising the steps of: Sowing after ditch application of arbuscular mycorrhizal fungi inoculant in the sowing period of the purple perilla, and respectively carrying out root irrigation treatment by using a composite inoculant containing bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and pseudomonas fluorescens (Pseudomonas fluorescens) in the seedling period and the current sequence period of the purple perilla; The arbuscular mycorrhizal fungi include rhizopus radiatus (Rhizophagus intraradices).
2. 2. The method according to claim 1, wherein the viable count of both the bacillus amyloliquefaciens and the pseudomonas fluorescens in the composite microbial agent is not less than 5 x 10 8 CFU/mL.
3. 3. The method of claim 2, wherein the bacillus amyloliquefaciens and the pseudomonas fluorescens are mixed in a bacterial liquid volume ratio of 1:1.
4. 4. The method of claim 1, wherein the amount of the composite microbial inoculum is 50 mL per plant.
5. 5. The method according to claim 1, wherein the number of effective propagules of rhizopus radiatus is equal to or greater than 70 per mL in the arbuscular mycorrhizal fungi agent.
6. 6. The method according to claim 1, wherein the arbuscular mycorrhizal fungi agent is applied in furrows after being uniformly mixed with wet fine soil during the sowing period of the perilla.
7. 7. The method of claim 1, further comprising the treatment of soil preparation and fertilization prior to seeding of the perilla.
8. 8. The method of claim 7, wherein the fertilizing comprises unifying use of a mature farmyard manure and a compound fertilizer.
9. 9. The method of claim 1, wherein the perilla quality comprises perilla seed oil content and alpha-linolenic acid content.
10. 10. A microbial composition for promoting the quality of perilla, which is characterized by comprising a composite microbial agent containing bacillus amyloliquefaciens and pseudomonas fluorescens and a arbuscular mycorrhizal fungus agent containing rhizopus radiatus.

Description

Method for promoting purple perilla quality improvement based on microbial composition Technical Field The invention relates to the technical field of plant cultivation, in particular to a method for promoting purple perilla quality improvement based on a microbial composition. Background Perilla frutescens (Perilla frutescens) is an important special oil crop in China, and the seeds of the perilla frutescens are rich in unsaturated fatty acids such as alpha-linolenic acid and the like, so that the perilla frutescens has high nutritional value and market demand. Improving the yield and quality of perilla seeds, in particular improving the oil content and the alpha-linolenic acid content of the perilla seeds, is an important direction of the development of the perilla industry. At present, the improvement of crop properties by adopting a microbiological technology is an important agronomic means besides variety breeding and conventional cultivation management. There are practices in the art to promote crop growth by applying microbial agents, but there are significant drawbacks when applied to the improvement of perilla quality. On the one hand, most of the existing methods focus on indirectly improving the yield by promoting the overall growth of plants through microorganisms, and lack of targeted regulation of a specific metabolic pathway of perilla seed oil synthesis and alpha-linolenic acid accumulation results in limited and unstable improvement effects on quality key indexes. On the other hand, the microorganism combination adopted in the prior art often has single or simple and overlapped functions, the synergistic action mechanism among all strains is not clear, and particularly, the function connection in different growth stages of the perilla is insufficient, so that the quality improvement effect of a system is difficult to form. In addition, the application scheme in the prior art is rough, and the accurate matching of the microbial activity and the critical waiting period of the perilla can not be fully considered, so that the fluctuation of the actual application effect is large. The essence of these problems is that the prior art fails to understand deeply the inherent link between the physiological and biochemical mechanisms of perilla quality formation and microbial regulation. Specifically, how to accurately regulate and control the carbon metabolism flow direction in different growth stages of perilla through microorganism combination, promote the efficient conversion of photosynthetic products to grain oil and fat, and specifically improve the metabolic intensity of an alpha-linolenic acid synthesis pathway is a difficulty faced by the current technology. The existing method often neglects the specific regulation and control effect of microorganisms in the key period of plant reproductive growth, and fails to solve the problem of synergy of microorganisms with different functions in field application. Therefore, development of a microorganism regulation and control method which can form a key link aiming at the quality of the purple perilla, and can accurately apply the microorganism regulation and control method at proper time through the synergistic effect of multiple strains has important significance for realizing high-yield and high-quality production of the purple perilla. Disclosure of Invention The invention aims to provide a method for promoting the improvement of the quality of purple perilla based on a microorganism composition so as to solve the problems in the prior art. According to the invention, the arbuscular mycorrhizal fungi and the specific composite bacterial microbial inoculum are applied step by step in the seeding, seedling and current sequence of the purple perilla, so that the cooperative and accurate regulation and control of the growth of the purple perilla and the metabolism of grease are realized, and the problem of unstable quality improving effect in the prior art is effectively solved. The method can synergistically improve the yield, the oil content and the alpha-linolenic acid content of the perilla seeds, and provides a stable and effective green solution for high-yield and high-quality production. In order to achieve the above object, the present invention provides the following solutions: The invention provides a method for promoting purple perilla quality improvement based on a microorganism composition, which comprises the following steps: Sowing after ditch application of arbuscular mycorrhizal fungi inoculant in the sowing period of the purple perilla, and respectively carrying out root irrigation treatment by using a composite inoculant containing bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and pseudomonas fluorescens (Pseudomonas fluorescens) in the seedling period and the current sequence period of the purple perilla; The arbuscular mycorrhizal fungi include rhizopus radiatus (Rhizophagus intraradices). Optionally, the number of via