CN-121986698-A - Cultivation method of cypripedium schneiderianum symbiotic protocorm

Abstract

The invention belongs to the technical field of orchid breeding, and particularly relates to a cultivation method of a shanxi cypripedium symbiotic protocorm, which comprises the following steps of preparing a nylon net film with the aperture of 30-80 mu m into a seed bag with a bag-shaped structure, filling shanxi cypripedium seeds into the seed bag according to the quantity of 50-200 grains per bag, sealing a bag opening, burying the seed bag in soil of a root zone of an adult plant of shanxi cypripedium protogenic land, covering a protozoon covering, and enabling the seeds to induce and form protocorms under natural symbiotic conditions. The method provided by the invention is simple and convenient to operate, and the obtained cypripedium schneiderianum symbiotic protocorm has natural symbiotic activity and excellent ecological adaptability, and can realize the efficient recovery of cypripedium schneiderianum symbiotic protocorm.

Inventors

• Liu Huanchu
• CHEN WEI
• WANG YANLIN
• HE XINGYUAN
• ZHANG YUE
• HUANG YANQING

Assignees

• 中国科学院沈阳应用生态研究所

Dates

Publication Date

20260508

Application Date

20260407

Claims (8)

1. 1. The method for cultivating the symbiotic protocorms of the cypripedium schneiderianum is characterized by comprising the following steps of: Preparing a nylon net film with the aperture of 30-80 mu m into a seed bag with a bag-shaped structure; Filling cypripedium schneiderianum seeds into the seed bags according to the quantity of 50-200 seeds per bag, and sealing the bag openings to obtain seed bags to be pre-buried; Burying the seed bag to be embedded in soil of a root zone of an adult plant of the cypripedium schneiderianum protozoon, and covering a protozoon covering, so that the seeds are induced to form protocorms under the condition of natural symbiosis.
2. 2. The method of claim 1, wherein the seed bag has a bag-like structure with three sealed surfaces and one open surface, and has a length of 5cm to 8cm and a width of 5cm to 8cm.
3. 3. The method according to claim 2, characterized in that the opening of the seed bag is provided with a drawstring for sealing.
4. 4. The method of claim 1, wherein the rhizosphere area is an area 0cm to 30cm from an adult plant of the cypripedium schneiderianum origin.
5. 5. The method of claim 1, wherein the buried depth is 2cm to 3cm.
6. 6. The method of claim 1, wherein the habitat cover comprises at least one of surface moss, soil, humus, and dead branch fallen leaves.
7. 7. The method of claim 1, wherein the seed bag is buried for a period of 9 months to 10 months.
8. 8. The method according to claim 1, further comprising recovering the obtained symbiotic protocorms of cypripedium macranthum in 8 months of the next year after burying and 8 months of the third year after burying.

Description

Cultivation method of cypripedium schneiderianum symbiotic protocorm Technical Field The invention belongs to the technical field of orchid plant breeding, and particularly relates to a cultivation method of a shanxi cypripedium symbiotic protocorm. Background Cypripedium schneiderianum (Cypripedium shanxiense) belongs to cypripedium genus of the family of the orchidaceae, and is a rare temperature-zone dried cypripedium schneiderianum. The flower shape is unique, the color is gorgeous, and the flower has extremely high ornamental value and gardening development potential. However, under the influence of the limitation of biological characteristics and the damage of the primordial environment, the natural population quantity of cypripedium schneiderianum is rare, the distribution area is narrow, and the development of the research on the protection of germplasm resources and artificial breeding thereof has important significance for maintaining the biodiversity and meeting the demand of gardening markets. Similar to other orchids, cypripedium schneiderianum seeds have typical biological characteristics of tiny seed volume, extremely degenerated or even absent endosperm, self-stored nutrient substances can not support independent germination and early growth of embryo, compact seed coat, poor water permeability and severe requirements on germination environment. Under natural conditions, the germination of cypripedium schneider seeds must rely on the invasion of specific mycorrhizal fungi to form symbiotic relationship, and the fungi provide carbon sources, nitrogen sources and other nutrients necessary for the germination of seeds and the formation of protocorms. Compared with tropical orchid, the germination of temperate-zone ground orchid is more difficult, the germination rate is low, and the formation period of protocorms is long, which becomes a core bottleneck for restricting the artificial breeding of the orchid. At present, seed germination and seedling breeding of cypripedium plants such as cypripedium schneid mainly depend on three technical paths, but obvious limitations exist. The aseptic seeding and tissue culture technology is the mainstream method for rapid propagation of orchid plants at present, and mature seeds are inoculated on an aseptic culture medium containing hormone, sugar and organic additives after surface sterilization, so as to induce the seeds to germinate and form protocorms. However, the seedlings obtained by the technology are separated from the natural microorganism environment for a long time, and lack of the capability of establishing connection with field symbiotic fungi, so that the transplanting survival rate is low, the environmental adaptability is poor, and effective field regression is difficult to realize. The fungus assisted symbiotic germination technology provides a new idea for solving the problems, and the method is characterized in that effective symbiotic mycorrhizal fungi are firstly separated and identified from roots of orchid plants, and then are co-cultured with seeds in a laboratory to induce symbiotic germination. The method has the advantages that the operation flow is extremely complex, a plurality of links are required to be established through field sampling, fungus separation and purification, strain identification, activity screening and co-culture system, the period is long, the success rate is low, in addition, the orchid has a high-specificity symbiotic relationship with mycorrhizal fungi, the effective fungi types depended on different habitats, populations or plants have differences, the screened strains cannot guarantee universality of all seeds, the technical requirement is high, and the popularization is difficult to be realized as a simple, convenient, efficient and general breeding means. The method for directly sowing the mature seeds in the original land directly sows the mature seeds in the soil under the forest of the original land, is closest to the natural state, but the field environment is complex and changeable, the seeds are easy to cause a large amount of loss due to rain wash, wind power transportation, animal feeding and mold infection, meanwhile, the seeds are distributed and dispersed in the soil, the seeds are difficult to effectively find and recover after germination to form protocorms, the germination period is long, the germination process cannot be effectively monitored and tracked, the germination rate is extremely low, the result is uncontrollable, the repeatability is poor, and a stable and predictable protocorm source cannot be provided for scientific research and breeding work. Therefore, the development of a method which can utilize native natural flora, does not need complex equipment, is simple and convenient to operate and can effectively induce and cultivate symbiotic protocorms has important practical significance for breaking through the propagation bottleneck of cypripedium macranthum and realizing th