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CN-121986710-A - Method for promoting pinus massoniana aerial culture rooting

CN121986710ACN 121986710 ACN121986710 ACN 121986710ACN-121986710-A

Abstract

The invention provides a method for promoting pinus massoniana to root by aeroponics, and relates to the technical field of pinus massoniana cutting propagation. The pinus massoniana aerial culture rooting method comprises the steps of soaking pinus massoniana scions in citric acid solution in ice water bath, disinfecting, treating with rooting agent, and carrying out aerial culture by adding calcium nitrate, potassium nitrate, magnesium sulfate, copper sulfate, indolebutyric acid, sodium phytate and laureth-4 nutritional agent. The invention overcomes the defects of the prior art, effectively promotes the branches of the pinus koraiensis to root rapidly, ensures the growth of root systems, provides a rapid and convenient method for stable seedling culture of pinus koraiensis, and ensures the artificial breeding of pinus koraiensis.

Inventors

  • CHEN WEI
  • LI JIANG
  • FENG XIAN
  • Fu Yupin
  • LUO TING
  • Hu Tanggeran

Assignees

  • 云南省林业和草原科学院

Dates

Publication Date
20260508
Application Date
20260410

Claims (9)

  1. 1. A method for promoting the rooting of pinus massoniana in a mist culture manner, which is characterized by comprising the following steps: s1, cutting tender scions of the pinus massoniana to serve as branches to be treated for standby; S2, soaking the 1-2cm lower end of the branch in 5% -7.5% citric acid solution ice water bath, airing, and cutting off the part of the lower end soaked in the citric acid solution, and keeping the lower end cut tidy to obtain a pretreated branch A for later use; s3, soaking the lower end of the pretreated branch A by using disinfectant, washing with clear water, soaking by using rooting agent, and airing to obtain a pretreated branch B for later use; S4, preparing a nutritional agent according to the following formula, wherein the nutritional agent comprises 400-500mg/L of calcium nitrate, 300-320mg/L of potassium nitrate, 120-200mg/L of magnesium sulfate, 1-1.6mg/L of copper sulfate, 50-100mg/L of indolebutyric acid, 20-40mg/L of sodium phytate, 8-16mg/L of laureth and the balance of water; S5, inserting the lower end of the pretreated branch B into a light-tight seedling tray hole, adding a nutrient into an atomizer, and starting the atomizer at intervals of 4-6h for 10-12min to perform aeroponic treatment on the lower end of the pretreated branch B.
  2. 2. The method of claim 1, wherein the length of the spike in step S1 is 15-18cm.
  3. 3. The method of claim 1, wherein in the step S1, pine needles 3-5cm below the scion are removed after scion cutting to obtain branches to be treated.
  4. 4. The method according to claim 1, wherein the lower end notch in the step S2 is a flush cut.
  5. 5. The method according to claim 1, wherein the time of the ice water bath soaking treatment in the step S2 is 20-30min.
  6. 6. The method of claim 1, wherein the disinfectant in the step S3 is 500-800 times carbendazim solution, the disinfectant soaking time is 10-15min, and the soaking position is 1-2cm below the branches.
  7. 7. The method of claim 1, wherein the rooting agent in the step S3 is 100mg/L of indoleacetic acid solution, the rooting agent is soaked for 1-2 hours, and the soaking position is 1-2cm below the lower ends of the branches.
  8. 8. The method according to claim 1, wherein the lower end of the pretreatment branch B in the step S5 is inserted into the hole of the light-proof seedling tray, and the lower end extends to 1-2cm below the hole.
  9. 9. The method according to claim 1, wherein the temperature is controlled to 15-28 ℃ and the humidity is controlled to 80% or more during the aeroponic treatment in the step S5.

Description

Method for promoting pinus massoniana aerial culture rooting Technical Field The invention relates to the technical field of cutting propagation of pinus massoniana, in particular to a method for promoting the rooting of pinus massoniana by means of aeroponic culture. Background The pinus koraiensis is one of special and fast-growing pinus koraiensis in Yunnan, has excellent material and wide application, is especially well known as long fiber materials, is suitable for producing high-grade paper pulp and paper products, has extremely important economic value and ecological significance, so that the pinus koraiensis is mainly planted artificially in order to meet the development of market economy. The propagation mode of the pinus koraiensis is mainly divided into sexual propagation and asexual propagation, wherein the sexual propagation is generally seed planting, but the offspring is easy to generate character separation when the seeds are used for propagation, the offspring cannot completely obtain the excellent characters of parents, the asexual propagation is mainly cutting propagation, which can effectively ensure that the seedlings inherit the excellent characters of woody, but the pinus koraiensis belongs to the tree species which are difficult to root by cutting, the traditional matrix cutting has lower overall rooting rate, the rooting time is long when the cutting is needed to take root for about 60-70 days, the fog culture seedling is a mode of soilless seedling, the nutrient solution is directly sprayed on the root system in a fog form, the nutrient absorption efficiency is improved, the growth of cutting slips is possibly promoted, the rooting of the cutting seedlings can be promoted to a certain extent by adjusting the components of the nutrient solution, meanwhile, the space is effectively saved, and the pinus koraiensis seedling is an important research direction at the current stage by adopting the fog culture mode. Disclosure of Invention Aiming at the defects of the prior art, the invention provides a method for promoting the mist culture rooting of the pinus massoniana, which effectively promotes the rapid rooting of the pinus massoniana branches, ensures the growth of root systems, provides a rapid and convenient method for the stable seedling culture of the pinus massoniana and ensures the artificial breeding of the pinus massoniana. In order to achieve the above purpose, the invention is realized by the following technical scheme: a method for promoting the rooting of pinus massoniana by aeroponics, comprising the following steps: s1, cutting tender scions of the pinus massoniana to serve as branches to be treated for standby; S2, soaking the 1-2cm lower end of the branch in 5% -7.5% citric acid solution ice water bath, airing, and cutting off the part of the lower end soaked in the citric acid solution, and keeping the lower end cut tidy to obtain a pretreated branch A for later use; s3, soaking the lower end of the pretreated branch A by using disinfectant, washing with clear water, soaking by using rooting agent, and airing to obtain a pretreated branch B for later use; S4, preparing a nutritional agent according to the following formula, wherein the nutritional agent comprises 400-500mg/L of calcium nitrate, 300-320mg/L of potassium nitrate, 120-200mg/L of magnesium sulfate, 1-1.6mg/L of copper sulfate, 50-100mg/L of indolebutyric acid, 20-40mg/L of sodium phytate, 8-16mg/L of laureth and the balance of water; S5, inserting the lower end of the pretreated branch B into a light-tight seedling tray hole, adding a nutrient into an atomizer, and starting the atomizer at intervals of 4-6h for 10-12min to perform aeroponic treatment on the lower end of the pretreated branch B. Preferably, the length of the spike in the step S1 is 15-18cm. Preferably, in the step S1, pine needles 3-5cm below the scion are removed after scion is cut to obtain branches to be treated. Preferably, in the step S2, the lower end incision is a straight incision. Preferably, the time of the ice water bath soaking treatment in the step S2 is 20-30min. Preferably, the disinfectant in the step S3 is 500-800 times carbendazim solution, the soaking time of the disinfectant is 10-15min, and the soaking position is 1-2cm below the branches. Preferably, in the step S3, the rooting agent is 100mg/L indoleacetic acid solution, the soaking time of the rooting agent is 1-2 hours, and the soaking position is 1-2cm below the branches. Preferably, the lower end of the pretreatment branch B in the step S5 is inserted into a hole of the light-tight seedling tray, and the lower end extends to 1-2cm below the hole. Preferably, the temperature is controlled to be 15-28 ℃ and the humidity is controlled to be more than 80% during the aeroponic treatment in the step S5. The invention provides a method for promoting the rooting of pinus massoniana in a mist culture way, which has the advantages that compared with the prior art: Accord