CN-121986714-A - Wheat drought-resistant breeding method and method for identifying or assisting in identifying wheat drought resistance

CN121986714ACN 121986714 ACN121986714 ACN 121986714ACN-121986714-A

Abstract

The invention discloses a wheat drought-resistant breeding method and a method for identifying or assisting in identifying wheat drought resistance. According to the invention, a near isogenic line NIL-TaSCE1 is constructed by taking a drought-resistant variety Shaan agricultural 138 as a donor parent and a drought-sensitive variety Lu wheat 23 as a recurrent parent, and a TaSCE1 Hap A haplotype containing a SNP1-SNP9 and a variation combination of two indels is introduced into a TaSCE1 Hap B haplotype drought-sensitive breeding material, so that the adaptability of wheat to drought environment can be improved, the drought resistance of the wheat can be improved, the influence of water stress on plant growth and development can be reduced, and grain yield reduction caused by environment change and other irresistible resistance can be solved under the premise that the yield of the recurrent parent breeding material is not influenced.

Inventors

HU ZHAORONG
SUN QIXIN
YANG QUN
SONG NA
NI ZHONGFU
XIN MINGMING
PENG HUIRU
Yao Yingken

Assignees

中国农业大学

Dates

Publication Date: 20260508
Application Date: 20241104

Claims (10)

1. A wheat breeding method is characterized by comprising the steps of hybridizing wheat A with wheat B to obtain wheat of F 1 generation, taking the wheat B as a recurrent parent, backcrossing the wheat of F 1 generation with the wheat B to obtain backcrossed offspring, selecting wheat containing haplotype Hap A from the backcrossed offspring to carry out selfing to obtain selfing offspring, and selecting wheat homozygous for the haplotype Hap A from the selfing offspring to obtain target wheat with drought resistance higher than that of the wheat B; The wheat A is the wheat containing the haplotype Hap A, and the wheat B is the wheat not containing the haplotype Hap A; the haplotype Hap A is a variant combination which does not contain InDel1 and InDel2 in a wheat genome, and has the SNP1 of C, SNP of A, SNP of T, SNP of A, SNP5 of G, SNP of 536 of A, SNP of T, SNP of C and the SNP9 of C; the SNP1 is a single nucleotide polymorphism site in a wheat genome, is C or T, and corresponds to the 97 th nucleotide of a sequence 1 in a sequence table; the SNP2 is a single nucleotide polymorphism site in a wheat genome, the single nucleotide polymorphism site is A or G, the 270 th nucleotide corresponding to a sequence 1 in a sequence table, the SNP3 is a single nucleotide polymorphism site in the wheat genome, the single nucleotide polymorphism site is T or A, the 574 th nucleotide corresponding to the sequence 1 in the sequence table, the SNP4 is a single nucleotide polymorphism site in the wheat genome, the single nucleotide polymorphism site is A or G, the 743 th nucleotide corresponding to the sequence 1 in the sequence table, the SNP5 is a single nucleotide polymorphism site in the wheat genome, the single nucleotide polymorphism site is G or T, the 846 th nucleotide corresponding to the sequence 1 in the sequence table, the single nucleotide polymorphism site is A or C, the 864 th nucleotide corresponding to the sequence 1 in the sequence table, the SNP7 is a single nucleotide polymorphism site in the wheat genome, the C is A or G, the single nucleotide polymorphism site is the 978 th nucleotide corresponding to the sequence 1 in the sequence table, the single nucleotide polymorphism site is the 1818 of the sequence 1 in the sequence table, the InDel1 corresponds to the 848 th-852 th nucleotide of the sequence 1 in the sequence table, and the InDel2 corresponds to the 989-1000 th nucleotide of the sequence 1 in the sequence table.
2. The method of claim 1, wherein the number of generations of backcrossing is equal to or greater than four.
3. The method according to claim 1 or 2, wherein the selection of the wheat containing haplotype Hap A and the selection of the wheat homozygous for haplotype Hap A comprises the step of amplifying genomic DNA of wheat to be detected by PCR using a primer composition comprising single-stranded DNA represented by sequence 2 in the sequence table, single-stranded DNA represented by sequence 3 in the sequence table, single-stranded DNA represented by sequence 4 in the sequence table and single-stranded DNA represented by sequence 5 in the sequence table, and sequencing the obtained PCR product.
4. The method according to claim 1 to 3, wherein the B-wheat is a drought-sensitive wheat material.
5. The use of the primer composition as claimed in claim 4 for identifying drought resistance of wheat.
6. The product for identifying the drought resistance of the wheat is characterized by being any one of the following: D1 PCR primers for detecting haplotype Hap a; D2 A PCR reagent comprising D1) the PCR primer; D3 A kit comprising D1) said PCR primer or D2) said PCR reagent; The haplotype Hap A is a variant combination which does not contain InDel1 and InDel2 in a wheat genome, and has SNP1 of C, SNP2 of A, SNP3 of T, SNP4 of A, SNP5 of G, SNP6 of A, SNP of T, SNP8 of C and SNP9 of C; the SNP1 is a single nucleotide polymorphism site in a wheat genome, is C or T, corresponds to the 97 th nucleotide of the sequence 1 in a sequence table, the SNP2 is a single nucleotide polymorphism site in the wheat genome, is A or G, corresponds to the 270 th nucleotide of the sequence 1 in the sequence table, the SNP3 is a single nucleotide polymorphism site in the wheat genome, is T or A, corresponds to the 574 th nucleotide of the sequence 1 in the sequence table, the SNP4 is a single nucleotide polymorphism site in the wheat genome, is A or G, corresponds to the 743 th nucleotide of the sequence 1 in the sequence table, is G or T, corresponds to the 846 th nucleotide of the sequence 1 in the sequence table, the SNP6 is a single nucleotide polymorphism site in the wheat genome, is A or A, corresponds to the 574 th nucleotide of the sequence 1 in the sequence table, the SNP4 is a single nucleotide polymorphism site in the sequence 1 in the sequence table, is a or G, corresponds to the 743 th nucleotide of the sequence 1 in the sequence table, the SNP5 is a single nucleotide polymorphism site in the sequence table, is G or T, corresponds to the nucleotide of the sequence 1 in the sequence table, the SNP6 is a single nucleotide polymorphism site in the sequence table, and the SNP8 is a single nucleotide polymorphism site in the sequence 1 in the sequence table, or the sequence 1 in the sequence table, the InDel1 corresponds to nucleotide numbers 848-852 [ Qiansong 1] [2] of the sequence 1 in the sequence table, and the InDel2 corresponds to nucleotide numbers 989-1000 of the sequence 1 in the sequence table.
7. The product of claim 6, wherein the PCR primer comprises a primer set consisting of single-stranded DNA represented by sequence 2 in the sequence table, single-stranded DNA represented by sequence 3 in the sequence table, single-stranded DNA represented by sequence 4 in the sequence table and single-stranded DNA represented by sequence 5 in the sequence table.
8. The method comprises detecting the genotype of the wheat to be detected, and identifying or assisting in identifying the drought resistance of the wheat to be detected according to the genotype, wherein the genotype is that the drought resistance of the wheat to be detected with the homozygous Hap A is higher than or the candidate of the wheat to be detected with the homozygous Hap B; The Hap A homozygote is a genotype of a homozygous variation of wheat genome without InDel1 and InDel2, and SNP1 is C, SNP, A, SNP, T, SNP4, A, SNP, G, SNP, A, SNP, T, SNP and C, and SNP9 is C; the Hap B homozygote is a genotype of a homozygous variation containing InDel1 and InDel2 in a wheat genome, wherein SNP1 is T, SNP2, G, SNP, A, SNP4, G, SNP5, T, SNP6, C, SNP, C, SNP and SNP9 is G; the SNP1 is a single nucleotide polymorphism site in a wheat genome, is C or T, corresponds to the 97 th nucleotide of the sequence 1 in a sequence table, the SNP2 is a single nucleotide polymorphism site in the wheat genome, is A or G, corresponds to the 270 th nucleotide of the sequence 1 in the sequence table, the SNP3 is a single nucleotide polymorphism site in the wheat genome, is T or A, corresponds to the 574 th nucleotide of the sequence 1 in the sequence table, the SNP4 is a single nucleotide polymorphism site in the wheat genome, is A or G, corresponds to the 743 th nucleotide of the sequence 1 in the sequence table, is G or T, corresponds to the 846 th nucleotide of the sequence 1 in the sequence table, the SNP6 is a single nucleotide polymorphism site in the wheat genome, is A or A, corresponds to the 574 th nucleotide of the sequence 1 in the sequence table, the SNP4 is A or G, corresponds to the 743 th nucleotide of the sequence 1 in the sequence table, the SNP5 is a single nucleotide polymorphism site in the sequence table, is G or T, corresponds to the nucleotide of the sequence 1 in the sequence table, the SNP6 is a single nucleotide polymorphism site in the sequence table, and the SNP8 is a single nucleotide polymorphism site in the sequence table, the SNP9 is a single nucleotide polymorphism site in a wheat genome, the single nucleotide polymorphism site is T or G, the single nucleotide polymorphism site corresponds to the 2902 th nucleotide of the sequence 1 in the sequence table, the InDel1 corresponds to the 848 th-852 th nucleotide of the sequence 1 in the sequence table, and the InDel2 corresponds to the 989 th-1000 th nucleotide of the sequence 1 in the sequence table.
9. Use of a substance that detects a haplotype that is a variation on one chromosome of a wheat genome of 9 SNPs (single nucleotide polymorphisms) and 2 indels (Insertion or Deletion, insertions or deletions) that are SNP1 to SNP9 and 2 indels that are InDel1 and InDel2, for the identification or assisted identification of drought resistance of wheat; The SNP1 is a single nucleotide polymorphism site in a wheat genome, is C or T, and corresponds to the 97 th nucleotide of a sequence 1 in a sequence table; the SNP2 is a single nucleotide polymorphism site in a wheat genome, the nucleotide is A or G, and the SNP2 corresponds to the 270 th nucleotide of a sequence 1 in a sequence table; the SNP3 is a single nucleotide polymorphism site in a wheat genome, the single nucleotide polymorphism site is T or A, the single nucleotide polymorphism site corresponds to 574 th nucleotide of a sequence 1 in a sequence table, the SNP4 is A or G, the single nucleotide polymorphism site corresponds to 743 th nucleotide of the sequence 1 in the sequence table, the SNP5 is a single nucleotide polymorphism site in the wheat genome, the single nucleotide polymorphism site corresponds to G or T, the single nucleotide polymorphism site corresponds to 846 th nucleotide of the sequence 1 in the sequence table, the SNP6 is A or C, the single nucleotide polymorphism site corresponds to 864 th nucleotide of the sequence 1 in the sequence table, the SNP7 is a single nucleotide polymorphism site corresponds to T or C, the single nucleotide polymorphism site corresponds to 978 th nucleotide of the sequence 1 in the sequence table, the SNP8 is a single nucleotide polymorphism site in the wheat genome, the nucleotide polymorphism site corresponds to G or T, the single nucleotide polymorphism site corresponds to 849 th nucleotide of the sequence 1 in the sequence table, the Del1 corresponds to the nucleotide of the sequence 1 in the sequence table, the SNP1 is a single nucleotide polymorphism site corresponds to the nucleotide of the sequence 1 in the sequence table, the sequence 1 in the sequence table is a or the Del1 in the sequence table is a nucleotide of the sequence 1, the sequence of the sequence 1 is a nucleotide of the sequence 1 is a or a nucleotide of the sequence 1.
10. Use of a substance that detects a haplotype that is a variation on one chromosome of a wheat genome of 9 SNPs (single nucleotide polymorphisms) and 2 indels (Insertion or Deletion, insertions or deletions) that are SNPs 1 to SNP9, and 2 indels that are InDel1 and InDel2, in the preparation of a product for identifying or aiding in the identification of wheat drought resistance; The SNP1 is a single nucleotide polymorphism site in a wheat genome, is C or T, and corresponds to the 97 th nucleotide of a sequence 1 in a sequence table; the SNP2 is a single nucleotide polymorphism site in a wheat genome, the nucleotide is A or G, and the SNP2 corresponds to the 270 th nucleotide of a sequence 1 in a sequence table; the SNP3 is a single nucleotide polymorphism site in a wheat genome, the single nucleotide polymorphism site is T or A, the single nucleotide polymorphism site corresponds to 574 th nucleotide of a sequence 1 in a sequence table, the SNP4 is A or G, the single nucleotide polymorphism site corresponds to 743 th nucleotide of the sequence 1 in the sequence table, the SNP5 is a single nucleotide polymorphism site in the wheat genome, the single nucleotide polymorphism site corresponds to G or T, the single nucleotide polymorphism site corresponds to 846 th nucleotide of the sequence 1 in the sequence table, the SNP6 is A or C, the single nucleotide polymorphism site corresponds to 864 th nucleotide of the sequence 1 in the sequence table, the SNP7 is a single nucleotide polymorphism site corresponds to T or C, the single nucleotide polymorphism site corresponds to 978 th nucleotide of the sequence 1 in the sequence table, the SNP8 is a single nucleotide polymorphism site in the wheat genome, the nucleotide polymorphism site corresponds to G or T, the single nucleotide polymorphism site corresponds to 849 th nucleotide of the sequence 1 in the sequence table, the Del1 corresponds to the nucleotide of the sequence 1 in the sequence table, the SNP1 is a single nucleotide polymorphism site corresponds to the nucleotide of the sequence 1 in the sequence table, the sequence 1 in the sequence table is a or the Del1 in the sequence table is a nucleotide of the sequence 1, the sequence of the sequence 1 is a nucleotide of the sequence 1 is a or a nucleotide of the sequence 1.

Description

Wheat drought-resistant breeding method and method for identifying or assisting in identifying wheat drought resistance Technical Field The invention belongs to the technical field of biology, and particularly relates to a wheat drought-resistant breeding method and a method for identifying or assisting in identifying wheat drought resistance. Background Wheat is one of the world's important food crops and drought is an abiotic stress factor affecting wheat yield, quality and planting range. Under drought stress, plants adapt to drought stress through a range of physiological and biochemical changes, such as stomatal closure, cell wall and membrane composition changes. Different regulatory factors exist in organisms to control various signal transduction, and further downstream gene expression is regulated to cope with drought stress. Therefore, the research on the related genes of the wheat drought stress response and the analysis on the action mechanism of the genes have important effects on improving the wheat yield and the variety. Disclosure of Invention The invention aims to solve the technical problems of how to screen wheat drought-resistant materials and/or how to carry out wheat drought-resistant breeding and/or how to identify or assist in identifying the drought resistance of wheat. In order to solve the technical problems, the invention firstly provides a wheat breeding method, which comprises the steps of hybridizing wheat A with wheat B to obtain wheat of F 1 generation, taking the wheat B as a recurrent parent, backcrossing the wheat of F 1 generation with the wheat B to obtain backcrossed offspring, selecting wheat containing haplotype Hap A from the backcrossed offspring to carry out selfing to obtain selfing offspring, and selecting wheat homozygous for haplotype Hap A from the selfing offspring to obtain target wheat with drought resistance higher than that of the wheat B; The wheat A is the wheat containing the haplotype Hap A, and the wheat B is the wheat not containing the haplotype Hap A; the haplotype Hap A is a variant combination which does not contain InDel1 and InDel2 in a wheat genome, and has the SNP1 of C, SNP of A, SNP of T, SNP of A, SNP5 of G, SNP of 536 of A, SNP of T, SNP of C and the SNP9 of C; the SNP1 is a single nucleotide polymorphism site in a wheat genome, is C or T, and corresponds to the 97 th nucleotide of a sequence 1 in a sequence table; the SNP2 is a single nucleotide polymorphism site in a wheat genome, the single nucleotide polymorphism site is A or G, the 270 th nucleotide corresponding to a sequence 1 in a sequence table, the SNP3 is a single nucleotide polymorphism site in the wheat genome, the single nucleotide polymorphism site is T or A, the 574 th nucleotide corresponding to the sequence 1 in the sequence table, the SNP4 is a single nucleotide polymorphism site in the wheat genome, the single nucleotide polymorphism site is A or G, the 743 th nucleotide corresponding to the sequence 1 in the sequence table, the SNP5 is a single nucleotide polymorphism site in the wheat genome, the single nucleotide polymorphism site is G or T, the 846 th nucleotide corresponding to the sequence 1 in the sequence table, the single nucleotide polymorphism site is A or C, the 864 th nucleotide corresponding to the sequence 1 in the sequence table, the SNP7 is a single nucleotide polymorphism site in the wheat genome, the C is A or G, the single nucleotide polymorphism site is the 978 th nucleotide corresponding to the sequence 1 in the sequence table, the single nucleotide polymorphism site is the 1818 of the sequence 1 in the sequence table, the InDel1 corresponds to the 848 th-852 th nucleotide of the sequence 1 in the sequence table, and the InDel2 corresponds to the 989-1000 th nucleotide of the sequence 1 in the sequence table. In the method, the SNP1 corresponds to the 678628434 th position on the 5 th chromosome of the wheat reference genome of China spring, the nucleotide is C or T, the SNP2 corresponds to the 678628261 th position on the 5 th chromosome of the wheat reference genome, the nucleotide is A or G, the SNP3 corresponds to the 678627957 th position on the 5 th chromosome of the wheat reference genome, the nucleotide is T or A, the SNP4 corresponds to the 678627788 th position on the 5 th chromosome of the wheat reference genome, the nucleotide is A or G, the SNP5 corresponds to the 678627685 th position on the 5 th chromosome of the wheat reference genome, the nucleotide is G or T, the SNP6 corresponds to the 678627667 th position on the 5 th chromosome of the wheat reference genome, the nucleotide is A or C, the SNP7 corresponds to the 678627558 th position on the 5 th chromosome of the wheat reference genome, the nucleotide is T or C, the SNP8 corresponds to the 678627788 th position on the 5 th chromosome of the wheat reference genome, the nucleotide is G or T, the SNP5 corresponds to the 35G or 35G, and the nucleotide is 35G or 35 of the F. In th