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CN-121986718-A - Cultivation method of ginseng polygonum multiflorum

CN121986718ACN 121986718 ACN121986718 ACN 121986718ACN-121986718-A

Abstract

The invention relates to the technical field of plant cultivation, and particularly discloses a cultivation method of ginseng fleece-flower roots, which comprises the steps of collecting healthy and well-grown ginseng fleece-flower roots for two years, cleaning and sterilizing, taking bud points on the surfaces of the root tubers, inoculating the bud points into a primary induction culture medium for primary induction differentiation culture to obtain sterile buds, trimming the sterile buds into bud stem small sections with the length of about 0.5-1 cm, inoculating side bud stem small sections into a secondary multiplication culture medium for secondary culture to form seedlings, inoculating the multiplication culture medium into an MS culture medium containing 2.0-5.0 mg/L BAP and 0.2-0.5 mg/L NAA, transferring the seedlings into a rooting culture medium for rooting culture to form robust seedlings, and carrying out domestication transplanting on the robust seedlings. In the cultivation method of the ginseng polygonum multiflorum, the ginseng polygonum multiflorum seedlings are obtained by adopting root tuber bud points for tissue culture, and the cultivation method has the advantages of health, strong disease resistance, high survival rate and the like, can completely preserve the components and characteristics of original mother seeds, and realizes healthy propagation and seedling culture.

Inventors

  • Chen Kanghuan
  • LI YANMIN
  • Su Rongjie

Assignees

  • 梧州台华精细农业科技有限责任公司

Dates

Publication Date
20260508
Application Date
20260325

Claims (10)

  1. 1. The cultivation method of the ginseng polygonum multiflorum is characterized by comprising the following steps of: (1) Collecting healthy and well-grown two-year-old root tuber of radix ginseng and polygonum multiflorum, and cleaning and sterilizing; (2) Taking bud points on the surface of a root tuber, and inoculating the bud points to a primary induction culture medium for primary induction differentiation culture to obtain a sterile bud, wherein the primary induction culture medium is an MS culture medium containing 1.0-3.5 mg/L BAP; (3) Trimming the sterile buds into bud stem segments with the length of about 0.5-1 cm, inoculating the side bud stem segments into a secondary multiplication medium for secondary culture to form seedlings, wherein the multiplication medium is an MS medium containing 2.0-5.0 mg/L BAP and 0.2-0.5 mg/L NAA; (4) Transferring the seedlings into a rooting culture medium for rooting culture to obtain robust seedlings; (5) Growing the strong seedlings out of the bottles, and planting the strong seedlings in planting cups filled with culture soil for domestication to obtain large seedlings; (6) Transplanting the domesticated seedlings to a field, and performing field management.
  2. 2. The method according to claim 1, wherein the rooting medium is 1/2MS medium containing 0.05-0.2 mg/LNAA.
  3. 3. The method according to claim 1, wherein the cultivation conditions in the steps (2), (3) and (4) are that the temperature is 23+ -2deg.C, the illumination intensity is 2000-3000 Lux, and the illumination time is 12-14 h/d.
  4. 4. The method according to claim 1, wherein the primary induction medium is an MS medium containing 1.0-3.5 mg/L BAP, 15-25 g/L glucose, 4-5.5 g/L agar, and 10-20 mg/L grape seed oil.
  5. 5. The method according to claim 1, wherein the proliferation medium is an MS medium containing 2.0-5.0 mg/L BAP, 0.2-0.5 mg/L NAA, 15-25 g/L glucose, 4-5.5 g/L agar, 12-25 mg/L grape seed oil.
  6. 6. The method according to claim 1, wherein the rooting medium is a 1/2MS medium containing NAA 0.05-0.2mg/L, glucose 18-25 g/L, agar 4-5.5 g/L, and polysaccharide of Polygonum multiflorum 0.3-0.5 mg/L, and the pH is 5-6.
  7. 7. The method for cultivating ginseng and polygonum multiflorum according to claim 1, wherein in the step (1), disinfection is carried out, namely, the cleaned tuberous roots are soaked in 70% ethanol solution for 1-2 min and washed clean by sterile water, and then the tuberous roots are soaked in 0.1% -0.2% mercuric chloride solution by mass percent for 5-10 min and washed clean by sterile water.
  8. 8. The method according to claim 1, wherein in the step (5), the strong young plant is removed from the bottle before the bottle is removed, the young plant is removed from the greenhouse, the young plant is placed under a sunshade net or a semi-pudendum for 1-2 days, the young plant is then planted in a planting cup filled with culture soil, the young plant is placed under the sunshade net or the semi-pudendum for 3-5 days, and then the sunlight exposure time is increased, and the young plant is progressed from 2 hours to a full sunlight environment within 5-7.
  9. 9. The cultivation method of ginseng fleece-flower root according to claim 8, wherein the cultivation soil comprises, by weight, 20-30 parts of volcanic rock fragments, 12-18 parts of red jade soil, 10-15 parts of decomposed pine needle soil, 2-4 parts of hydroxyapatite and 1-3 parts of trehalose.
  10. 10. The cultivation method of ginseng fleece-flower root according to claim 9, wherein the raw materials are mixed, and water is added to the mixture to obtain nutrient soil, and the humidity of the nutrient soil is 60-70%.

Description

Cultivation method of ginseng polygonum multiflorum Technical Field The invention relates to the technical field of plant planting, in particular to a cultivation method of ginseng polygonum multiflorum. Background The ginseng fleece-flower root is a perennial winding herbal plant, the vine part of the ginseng fleece-flower root is the traditional Chinese medicine 'tuber fleeceflower stem', and the ginseng fleece-flower root has the effects of nourishing the heart, soothing the nerves, dispelling wind and dredging collaterals. The underground root tuber is called ginseng fleece-flower root after being processed, has bitter and slightly warm nature, can nourish liver and kidney, benefit essence and blood, strengthen tendons and bones, blacken hair, has nourishing and strengthening functions, and is also commonly used for treating insomnia. The root tuber of radix Polygoni Multiflori contains active ingredients such as ginsenoside, ginseng polysaccharide, volatile oil, amino acids, inorganic elements, peptides, vitamins, organic acids, alkaloids, flavone, enzymes, etc., especially phenols and sugar phenols. The darker the root tuber skin, the stronger the fragrance, and the longer the cultivation period, the higher the value, so the method is called as "black gold". The ginseng fleece-flower root is discovered in the mountain tribe of taiwan for the first time, and subsequent plant scholars are researched for many years, and the ginseng fleece-flower root of a new variety is cultivated by screening, hybridization optimization and improvement in a wild variety, and the ginseng fleece-flower root of the new variety is widely planted in taiwan and becomes a local characteristic product. The existing cultivation system depends on asexual reproduction, cutting reproduction or rhizome reproduction for a long time, and the existing cultivation system generally adopts the commercially eliminated defective tubers or the non-screened vine cutting seedlings as seed sources, so that the problems of germplasm degradation, reduced survival rate, reduced tuber biomass, obviously reduced disease and pest resistance, serious diseases and the like are easily caused, and the medicinal quality of the ginseng polygonum multiflorum is affected. Disclosure of Invention Aiming at the defects, the invention provides a cultivation method of ginseng fleece-flower root, which can remove viruses, has strong disease resistance and high survival rate by cultivating ginseng fleece-flower root seedlings, and has the following specific technical scheme: A cultivation method of radix ginseng polygoni multiflori comprises the following steps: (1) Collecting healthy and well-grown two-year-old root tuber of radix ginseng and polygonum multiflorum, and cleaning and sterilizing; (2) Taking bud points on the surface of a root tuber, and inoculating the bud points to a primary induction culture medium for primary induction differentiation culture to obtain a sterile bud, wherein the primary induction culture medium is an MS culture medium containing 1.0-3.5 mg/L BAP (6-benzyl adenine); (3) Trimming the sterile buds into bud stem segments with the length of about 0.5-1 cm, and inoculating the side bud stem segments into a secondary proliferation culture medium for secondary culture to form seedlings, wherein the secondary proliferation culture medium is an MS culture medium containing 2.0-5.0 mg/L BAP and 0.2-0.5 mg/L NAA (naphthalene acetic acid); (4) Transferring the seedlings into a rooting culture medium for rooting culture to obtain robust seedlings; (5) Growing the strong seedlings out of the bottles, and planting the strong seedlings in planting cups filled with culture soil for domestication to obtain large seedlings; (6) Transplanting the domesticated seedlings to a field, and performing field management. Preferably, in the cultivation method of ginseng fleece-flower root, the rooting culture medium is a 1/2MS culture medium containing 0.05-0.2mg/LNAA, and the pH is 5-6. Preferably, in the above cultivation method of ginseng fleece-flower root, in the steps (2), (3) and (4), the cultivation condition is that the temperature is 23±2 ℃, the illumination intensity is 2000 to 3000lux, and the illumination time is 12 to 14 hours/d. Preferably, in the cultivation method of ginseng fleece-flower root, the primary induction culture medium is an MS culture medium containing 1.0-3.5 mg/L BAP, 15-25 g/L glucose, 4-5.5 g/L agar and 10-20 mg/L grape seed oil, and the pH is 5-6. Grape seed oil is added into the primary induction culture medium, and the grape seed oil contains a large amount of saturated fatty acids, various vitamins, antioxidant substances (such as polyphenols) and the like, so that the stress resistance of the bud point tissue can be improved, the cell repair is accelerated, the degradation of nutrient substances is slowed down, and the differentiation of the bud point tissue is promoted. Preferably, in the cultivation method of ginseng