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CN-121986758-A - Experimental device for research insect olfactory behavior

CN121986758ACN 121986758 ACN121986758 ACN 121986758ACN-121986758-A

Abstract

The invention relates to the technical field of insect olfactory behavior experiments and discloses an experimental device for researching insect olfactory behaviors, which comprises a base, a video recording device, an air inlet box, an annular pipe, an air supply assembly and a plurality of gas storage assemblies, wherein a support is fixed on the base, the video recording device is arranged on the support, the air inlet box is fixed at the top end of the support, the annular pipe is fixed on the top surface of the base, the air supply assembly is arranged on the base, the plurality of gas storage assemblies are filled with different types of test gases respectively, the plurality of gas storage assemblies are arranged on the base, and the plurality of gas storage assemblies are distributed in a circumferential array along the annular pipe. According to the invention, a plurality of gas cylinders are used for storing different kinds of test gases, and a plurality of odor compounds can be tested at the same time, so that the screening of olfactory behavior reactions of insects to different odors is facilitated, and the screening efficiency of the odor compounds is improved.

Inventors

  • LIU JIANBAI
  • LI JIHU
  • MAO YONGKAI
  • Yi Jiequn
  • LIN MINGJIANG
  • WU HAN
  • XU HANLIANG
  • CHEN LIJUAN
  • YANG TING

Assignees

  • 广东省科学院南繁种业研究所

Dates

Publication Date
20260508
Application Date
20240219

Claims (10)

  1. 1. An experimental device for studying olfactory behavior of insects, comprising: The device comprises a base (1), wherein a bracket (2) is fixed on the base (1); A video recording device (3), the video recording device (3) being mounted on a stand (2); the air inlet box (4) is fixed at the top end of the bracket (2); the annular pipe (5) is fixed on the top surface of the base (1); the air supply assembly is arranged on the base (1); the gas storage assemblies are filled with different types of test gases respectively, are arranged on the base (1) and are distributed in a circumferential array along the annular tube (5); The two opening and closing components are arranged vertically opposite to each other, and are connected through a connecting cylinder (10); the two driving assemblies are respectively arranged on the two opening and closing assemblies; The two observing assemblies are respectively arranged on the two opening and closing assemblies; The three communication assemblies are respectively arranged on the two observation assemblies, and the other communication assembly is arranged on the air inlet box (4); The rotating assembly is arranged in the middle of the top surface of the base (1), and the two opening and closing assemblies and the two observing assemblies are assembled on the rotating assembly.
  2. 2. An experimental device for studying olfactory behavior of an insect according to claim 1, wherein said air supply assembly comprises: an air pump (61), the air pump (61) being mounted on the base (1); the two purifying bottles (62), the two purifying bottles (62) are arranged on the base (1), and active carbon is filled in the two purifying bottles (62); the air inlet end of the air pump (61) is communicated with one of the purifying bottles (62), the air outlet end of the air pump (61) is communicated with the other purifying bottle (62), an exhaust pipe is arranged on the purifying bottle (62) communicated with the air outlet end of the air pump (61), and the exhaust pipe is communicated with the annular pipe (5).
  3. 3. An experimental set for studying the olfactory behavior of an insect in accordance with claim 1, wherein said gas storage assembly comprises: the gas cylinder (71), the said gas cylinder (71) is placed on base (1), and the gas cylinder (71) stores the test gas; The first communication pipe (72), one end of the first communication pipe (72) is communicated with the gas cylinder (71), and the other end of the first communication pipe is communicated with the gas inlet box (4); A second communicating pipe (73), wherein one end of the second communicating pipe (73) is communicated with the gas cylinder (71), and the other end of the second communicating pipe is communicated with the annular pipe (5); two solenoid valves (74), the two solenoid valves (74) are installed respectively on the first communicating pipe (72) and the second communicating pipe (73).
  4. 4. An experimental device for studying olfactory behavior of an insect in accordance with claim 1, wherein said opening and closing assembly comprises: A fixing ring (81), wherein the fixing ring (81) is connected with the connecting cylinder (10); a rotating ring (82), the rotating ring (82) is rotatably connected to the fixed ring (81); a plurality of slide ways (83), wherein the slide ways (83) are all arranged on the rotating ring (82), and the slide ways (83) are distributed in a circumferential array; A plurality of opening and closing pieces (84), wherein the opening and closing pieces (84) are arranged between the fixed ring (81) and the rotating ring (82) in a sliding way; a plurality of connecting rods (85), wherein the connecting rods (85) are respectively fixed on the plurality of opening and closing sheets (84); A plurality of limiting blocks (86), wherein the limiting blocks (86) are respectively fixed on the plurality of opening and closing sheets (84); The limiting grooves (87) are formed in the fixing rings (81), and the limiting blocks (86) are respectively and slidably connected in the limiting grooves (87).
  5. 5. An experimental device for studying olfactory behavior of an insect in accordance with claim 4, wherein said drive assembly comprises: a motor (91), the motor (91) being mounted on the stationary ring (81); A gear (92), the gear (92) being fixed on the output shaft of the motor (91); The gear ring (93), gear ring (93) is fixed to be overlapped on swivel becket (82), intermeshing between gear (92) and gear ring (93).
  6. 6. An experimental set up for studying the olfactory behavior of an insect according to claim 4, wherein said observation assembly comprises: The observation tube (111), the observation tube (111) is fixed on the rotary ring (82), one end of the observation tube (111) is of a closed structure, the other end of the observation tube is of an open structure, and the open end of the observation tube (111) is opposite to the rotary ring (82); an observation window (112), the observation window (112) being provided on the outer peripheral surface of the observation tube (111); an opening (113), the opening (113) being provided on the outer peripheral surface of the observation tube (111); and a mesh plate (114), wherein the mesh plate (114) is fixed on the inner surface of the opening (113).
  7. 7. An experimental set up for studying the olfactory behavior of an insect according to claim 1, wherein said communicating means comprises: a first flange (121); The first flange (121) and the second flange (122) are arranged in a vertically opposite mode; a bellows (123) disposed between the first flange (121) and the second flange (122); The guide rods (124) are fixed on the first flange plate (121), and the second flange plate (122) is sleeved on the guide rods (124) in a sliding way; and the magnetic ring (125), wherein the magnetic ring (125) is fixed on the second flange plate (122).
  8. 8. An experimental device for studying olfactory behavior of an insect according to claim 1, wherein said rotating assembly comprises: The fixing frame (131), the fixing frame (131) is fixed on the top surface of the base (1); the two bearing seats (132), the two bearing seats (132) are fixed on the fixing frame (131); The two rotating shafts (133) are respectively assembled on the two bearing seats (132) in a rotating way, and one ends, close to each other, of the two rotating shafts (133) are fixedly connected with the connecting cylinder (10); a motor (134), the motor (134) being mounted on the mount (131); The speed reducer (135), the speed reducer (135) is installed on the fixing frame (131), and the output end of the speed reducer (135) is fixedly connected with one of the rotating shafts (133); And the input end of the speed reducer (135) is in transmission connection with the driving shaft of the motor (134) through the transmission piece (136).
  9. 9. An experimental device for investigating the olfactory behaviour of an insect according to claim 6, characterized in that said video recording means (3) are arranged directly opposite to the two observation barrels (111).
  10. 10. An experimental device for investigating the olfactory behaviour of an insect as claimed in claim 8, characterized in that said transmission means (136) are a chain transmission.

Description

Experimental device for research insect olfactory behavior Technical Field The invention relates to the technical field of insect olfactory behavior experiments, in particular to an experimental device for researching insect olfactory behaviors. Background Experimental devices for studying insect olfactory behavior typically involve a series of designs to simulate the ability of an insect to perceive and respond to odors. The experimental device for researching the olfactory behaviors of insects in the prior art has at least the following defects when in actual use: (1) Most experiments use Y-shaped tubes or T-maze to conduct insect olfactory behavior experiments, but the experiments can only test one or two odor compounds at the same time, which limits the broad-spectrum screening of insect olfactory sensitivity, because they cannot test multiple compounds at the same time, and increases the initial screening workload of target substances; (2) Aiming at insect olfaction experiments, the prior art needs an experimenter to operate for a long time in the experimental process, including placing insects in an experimental device, observing and recording olfaction behaviors of the insects, and the like, and the tedious operation is time-consuming and labor-consuming, and can increase experimental errors; (3) The prior art has limited applicability to large-scale olfactory screening work, and is difficult to realize high-throughput olfactory behavior screening because only a limited number of odorous compounds can be tested, so that the capability of comprehensively testing a plurality of samples in a short time is limited; (4) Part of the experiments require experimenters to manually record the olfactory behaviors of insects, which may introduce subjectivity and inconsistency, and increase difficulty in data collection, and the lack of an automated data collection system may limit accuracy and repeatability of data. Therefore, it is necessary to design an experimental device for studying the olfactory behaviors of insects to solve the above problems. Disclosure of Invention The invention aims to solve the defects in the prior art, and provides an experimental device for researching the olfactory behaviors of insects. In order to achieve the above purpose, the present invention adopts the following technical scheme: An experimental device for researching insect olfactory behaviors comprises a base, wherein a bracket is fixed on the base; a video recording device mounted on the stand; the air inlet box is fixed at the top end of the bracket; the annular pipe is fixed on the top surface of the base; the air supply assembly is arranged on the base; The gas storage assemblies are respectively filled with different types of test gases, are arranged on the base and are distributed in a circumferential array along the annular tube; The two opening and closing components are arranged vertically opposite to each other, and are connected through a connecting cylinder; the two driving assemblies are respectively arranged on the two opening and closing assemblies; The two observing assemblies are respectively arranged on the two opening and closing assemblies; the three communication assemblies are respectively arranged on the two observation assemblies, and the other communication assembly is arranged on the air inlet box; and the rotating assembly is arranged in the middle of the top surface of the base, and the two opening and closing assemblies and the two observing assemblies are assembled on the rotating assembly. As a preferred embodiment of the present invention, the air supply assembly includes: the air pump is arranged on the base; the two purifying bottles are arranged on the base, and activated carbon is filled in the two purifying bottles; The inlet end of the air pump is communicated with one of the purifying bottles, the outlet end of the air pump is communicated with the other purifying bottle, an exhaust pipe is arranged on the purifying bottle communicated with the outlet end of the air pump, and the exhaust pipe is communicated with the annular pipe. As a preferred embodiment of the present invention, the gas storage unit includes: The gas cylinder is arranged on the base, and test gas is stored in the gas cylinder; one end of the first communication pipe is communicated with the gas cylinder, and the other end of the first communication pipe is communicated with the gas inlet box; one end of the second communicating pipe is communicated with the gas cylinder, and the other end of the second communicating pipe is communicated with the annular pipe; the two electromagnetic valves are respectively arranged on the first communicating pipe and the second communicating pipe. As a preferred embodiment of the present invention, the opening and closing assembly includes: the fixed ring is connected with the connecting cylinder; the rotating ring is rotationally connected to the fixed ring; the sliding ways are arr