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CN-121986761-A - Method for inhibiting castor silkworm egg development

CN121986761ACN 121986761 ACN121986761 ACN 121986761ACN-121986761-A

Abstract

The invention discloses a method for inhibiting the development of castor silkworm eggs, and belongs to the technical field of agricultural biology. The method aims at the technical problems that the castor silkworms have multiple generations in one year, the raising cost is high and the breeding risk is high due to the diapause-free characteristic, and provides a technical scheme for delaying hatching by accurately controlling the low-temperature treatment time and parameters. The method is characterized in that castor silkworms lay eggs at 25-26 ℃, are cultivated at 25-26 ℃ for 15-20 hours after laying eggs, are subjected to cooling treatment, are subjected to cold storage treatment in a low-temperature environment of 5-8 ℃ for 5-6 d, and are finally transferred to a standard hatching environment of 25-28 ℃ and relative humidity of 75% -85% to resume development until hatching. The method can effectively prolong the silkworm egg period of castor, reduce annual breeding algebra, facilitate the adjustment of the breeding time, remarkably reduce the breeding cost and risk, and simultaneously maintain good hatchability and vitality.

Inventors

  • PU YUEXIA
  • Lv Mengqi
  • TAN FUYANG
  • LI FENGYE
  • HUANG WENGONG
  • BI LIHUI
  • WEI BOYOU
  • LIU YANWEI
  • AN CHUNMEI
  • HUANG LINGLI
  • SU HONGMEI
  • LUO QUN
  • WU JINGYAN
  • MENG YIYING
  • TAO JIYANG
  • HUANG LING
  • HE JI
  • LI CHEN
  • HUANG HONGYAN
  • TANG SHUNMING
  • LAN YANNI
  • HUANG YANGYU
  • LAN BIZHONG
  • ZHU CHANGJIE
  • LIU JUAN

Assignees

  • 广西壮族自治区蚕业技术推广站(广西壮族自治区蚕种质量检验检疫站、广西壮族自治区蚕业科学研究院)

Dates

Publication Date
20260508
Application Date
20260130

Claims (8)

  1. 1. A method for inhibiting the development of castor silkworm eggs, comprising the steps of: S1, spawning at 25-26 ℃, culturing at 25-26 ℃ for 15-20 hours after spawning, then cooling, and finally refrigerating the castor silkworm eggs in a low-temperature environment at 5-8 ℃ for 5-6 d; and S2, removing the castor silkworm eggs from the refrigeration environment, transferring the castor silkworm eggs into a standard hatching environment with the temperature of 25-28 ℃ and the relative humidity of 75-85%, and recovering the development, and continuously culturing the castor silkworm eggs in the standard hatching environment until the castor silkworm eggs hatch.
  2. 2. The method for inhibiting the development of castor silkworm eggs according to claim 1, wherein the cooling treatment adopts a staged cooling mode, specifically: Gradually cooling the cultivated castor silkworm eggs to a first temperature environment of 15-20 ℃ from 25-26 ℃ at a rate of 1.5-2.5 ℃ per hour, and keeping the temperature at a constant temperature, wherein the total time is 6 hours from the start of cooling to the end of keeping the temperature at the first temperature environment; Then cooling to a second temperature environment of 10-15 ℃ at a rate of 1.5-2.5 ℃ per hour, and keeping at a constant temperature after reaching a target temperature, wherein the total time is 6 hours from the start of cooling to the end of keeping at the constant temperature of the second temperature environment; And finally, cooling from 10-15 ℃ to 5-8 ℃ at the rate of 0.5-1 ℃ per hour, and refrigerating in the third temperature environment for 5-6 d after cooling to the target temperature.
  3. 3. The method for inhibiting the development of castor silkworm eggs according to claim 2, wherein the intermittent strong air flow stimulation treatment is carried out during the refrigeration treatment in the third temperature environment, in particular: And (3) applying a clean air flow with the flow rate of 0.5-1.0 m/s to the environment where the castor silkworm eggs are positioned every 48-60 h in the refrigerating period of 5-8 ℃ continuously, and stimulating for 1-3 h, wherein the temperature of the clean air flow is consistent with the environmental temperature of the third temperature, and is 5-8 ℃.
  4. 4. A method for inhibiting the development of castor silkworm eggs according to claim 3, wherein the components of the ambient gas are synchronously regulated and controlled during each strong airflow stimulation treatment, in particular: in the airflow stimulation environment, maintaining the oxygen volume concentration to be more than 20%, and simultaneously controlling the carbon dioxide volume concentration to be less than 0.04%; the gas component regulation and control is started 30min before the gas flow stimulation treatment is started and is continuously maintained during the whole gas flow stimulation period, and the active control of the gas concentration is stopped after the gas flow stimulation treatment is finished, so that the gas environment is restored to the atmospheric normal state.
  5. 5. The method for inhibiting the development of castor silkworm eggs according to claim 4, wherein the humidity gradient control is synchronously implemented during each strong airflow stimulation treatment, in particular: maintaining the relative humidity of the environment at 65% -70% in the first 30min of the airflow stimulation treatment; Maintaining the relative humidity of the environment at 70% -75% in 30-90 min of airflow stimulation treatment; gradually increasing the relative humidity of the environment to 80% -85% within 90-150 min of the airflow stimulation treatment, and keeping the relative humidity stable; The humidity is increased from 65% -70% to 80% -85%, and the humidity change rate is 8% -12% per hour; 15min before the air flow stimulation treatment is finished, the relative humidity is adjusted back to 75% -80%.
  6. 6. The method for inhibiting the development of castor silkworm eggs according to claim 5, wherein the multi-axis linear oscillation is synchronously implemented during the strong air flow stimulation treatment, in particular: During the 30 th to 90 th min of airflow stimulation treatment, carrying out 1 time of synchronous linear oscillation treatment every 10min, continuously carrying out 1min of each oscillation treatment, and carrying out two axial oscillations which are perpendicular to each other in a horizontal plane at the frequency of 1 to 2Hz and the amplitude of 0.5 to 1mm during the treatment; And (3) during the 90 th min to the end of the airflow stimulation treatment, the oscillation frequency is raised to 2-3 Hz, the amplitude is maintained to be 0.5-1 mm, the oscillation direction is periodically changed at a rate of 1 time every 10min in the horizontal plane, and the change angle is 30-45 degrees.
  7. 7. Method for inhibiting the development of castor silkworm eggs according to claim 6, characterized in that during multiaxial linear oscillation the castor silkworm eggs are placed on a support layer with suspension, in particular: The suspension supporting layer is made of a silica gel sponge material, the thickness of the suspension supporting layer is 3-5 mm, the Shore hardness of the suspension supporting layer is 10-15, through air holes with the aperture of 0.5-1 mm are uniformly distributed on the surface of the suspension supporting layer, and the density of the air holes is 25-40 holes/cm 2 per square centimeter; when multi-axis linear oscillation is implemented, the suspension supporting layer is fixed on the oscillation platform, castor silkworm eggs are tiled on the surface of the supporting layer in a single-layer mode, and the distance between egg grains is kept at 1.5-2 mm.
  8. 8. The method for inhibiting the development of castor silkworm eggs according to claim 7, wherein the negative ions are simultaneously injected into the air stream during the strong air stream stimulation treatment, specifically, the negative ions are continuously injected into the clean air stream at the beginning of the air stream stimulation treatment, so that the concentration of the negative ions is maintained at 1000-5000 ions/cm 3 and continuously maintained during the whole strong air stream stimulation period, wherein the negative ion injection is realized by a negative ion generator integrated in the air stream conveying pipeline.

Description

Method for inhibiting castor silkworm egg development Technical Field The invention belongs to the technical field of agricultural biology, and particularly relates to a method for inhibiting the development of castor silkworm eggs. Background At present, castor silkworms can generate multiple generations in one year under proper environmental conditions, and the uninterrupted growth and development characteristics of the castor silkworms lead to continuous feeding throughout the year in the breeding process of varieties. The method not only remarkably increases the investment of manpower and material resources, but also enables the silkworms to be exposed to environmental fluctuation and disease transmission risks for a long time, and the overall culture cost and uncertainty are high. As the castor silkworms belong to non-diapause insects, eggs of the castor silkworms cannot enter diapause states under natural conditions, the castor silkworms cannot suspend development by inducing diapause like tussah silkworms and control feeding rhythm by flexibly adjusting incubation time. In actual production, if an effective development inhibition means is lacking, the incubation time of silkworm eggs cannot be regulated, and the production plan, facility turnover and market demand are difficult to adjust. For a long time, the industry expects to realize the artificial control of the development process of the castor silkworm eggs through an environment-friendly and simple-to-operate physical method, so that the egg period is effectively prolonged and the breeding times are reduced on the premise of not affecting the subsequent hatching quality and the growth and development. However, there is a difficulty in achieving this objective in that if the timing of treatment is improper or conditions are not appropriate, it is likely to cause a decrease in embryo viability, a decrease in hatchability, or even dysplasia, and it is difficult to achieve a reliable balance between "effective inhibition" and "maintenance of embryo activity". Therefore, it is necessary to explore a method for precisely grasping the processing window and the low-temperature parameters, and to perform the intervention in the specific embryo sensitive stage, so as to achieve the purpose of delaying hatching. Disclosure of Invention It is an object of the present invention to solve at least the above problems and to provide at least the advantages to be described later. The invention also aims to provide a method for inhibiting the development of the castor silkworm eggs, which can effectively induce the castor silkworm embryos to enter a development inhibition state by controlling the time and the duration of low-temperature treatment, thereby safely prolonging the egg period, reducing the breeding algebra and obviously reducing the breeding risk and the cost. To achieve these objects and other advantages and in accordance with the purpose of the invention, there is provided a method of inhibiting the development of castor silkworm eggs, comprising the steps of: S1, spawning at 25-26 ℃, culturing at 25-26 ℃ for 15-20 hours after spawning, then cooling, and finally refrigerating the castor silkworm eggs in a low-temperature environment at 5-8 ℃ for 5-6 d; and S2, removing the castor silkworm eggs from the refrigeration environment, transferring the castor silkworm eggs into a standard hatching environment with the temperature of 25-28 ℃ and the relative humidity of 75-85%, and recovering the development, and continuously culturing the castor silkworm eggs in the standard hatching environment until the castor silkworm eggs hatch. After the castor silkworms spawn, selecting early embryo development, culturing for 15-20 hours at 25-26 ℃, then cooling, then placing castor silkworms in a low-temperature environment at 5-8 ℃ for refrigeration treatment for 5-6 d, and finally transferring the castor silkworms to a standard hatching environment at 25-28 ℃ and with relative humidity of 75% -85% for restoring development until hatching. The method of the invention utilizes the sensibility of the embryo at the specific development stage to low temperature, and induces the embryo to enter a development inhibition state through a continuous low-temperature environment, thereby realizing the artificial delay control of the hatching process. The method can safely and effectively prolong the egg period of the castor silkworms, thereby reducing the actual breeding algebra, obviously reducing the cost and risk in the breeding process, and simultaneously, the silkworm eggs can still recover the normal development process and keep good hatchability and vitality after the low-temperature inhibition is relieved due to proper treatment time and parameters. The whole method only depends on a physical temperature control means, and has the advantages of simple operation, safety and innocuity. Preferably, the cooling treatment adopts a staged cooling mode, and specifically com