Search

CN-121986784-A - Trans-2-hexenal oil-in-water nanoemulsion and preparation method and application thereof

CN121986784ACN 121986784 ACN121986784 ACN 121986784ACN-121986784-A

Abstract

The invention provides a trans-2-hexenal oil-in-water nanoemulsion and a preparation method and application thereof, and belongs to the technical field of biology. The nanoemulsion provided by the invention is prepared from the following raw materials of trans-2-hexenal, water and an emulsifying agent, wherein the emulsifying agent is Tween-80 and Span-80, the mass ratio of the Tween-80 to the Span-80 is 1 (5-9), the content of the emulsifying agent in the trans-2-hexenal oil-in-water nanoemulsion is 0.9-25 wt%, and the content of the trans-2-hexenal is 0.2-50 wt%. According to the invention, trans-2-hexenal is used as an oil phase, water and an emulsifying agent are added to prepare the oil-in-water nanoemulsion, and meanwhile, the type and the dosage of the emulsifying agent are controlled, so that the nanoemulsion can exist stably and is not layered, and compared with the method that trans-2-hexenal is directly adopted, the trans-2-hexenal oil-in-water nanoemulsion has a better sprouting inhibition effect on the arthrodesis seeds.

Inventors

  • YU HAIYAN
  • LI XIANGJU
  • CUI HAILAN
  • CHEN JINGCHAO
  • Wei Cunyu

Assignees

  • 中国农业科学院植物保护研究所

Dates

Publication Date
20260508
Application Date
20260408

Claims (10)

  1. 1. The trans-2-hexenal oil-in-water nanoemulsion is characterized by comprising the following raw materials of trans-2-hexenal, water and an emulsifier; the emulsifying agent is Tween-80 and Span-80, and the mass ratio of the Tween-80 to Span-80 is 1 (5-9); The content of the emulsifier in the trans-2-hexenal oil-in-water nanoemulsion is 0.9-25wt%, and the content of the trans-2-hexenal is 0.2-50wt%.
  2. 2. The trans-2-hexenal oil-in-water nanoemulsion of claim 1, wherein the raw materials of the trans-2-hexenal oil-in-water nanoemulsion further comprise a co-emulsifier.
  3. 3. The trans-2-hexenal oil-in-water nanoemulsion of claim 2, wherein the co-emulsifier comprises sodium alginate or chitosan.
  4. 4. The trans-2-hexenal oil-in-water nanoemulsion of claim 3, wherein when the co-emulsifier is sodium alginate, the content of sodium alginate in the trans-2-hexenal oil-in-water nanoemulsion is less than or equal to 0.05wt%.
  5. 5. The trans-2-hexenal oil-in-water nanoemulsion according to claim 3, wherein when the co-emulsifier is chitosan, the content of chitosan in the trans-2-hexenal oil-in-water nanoemulsion is not less than 0.45wt%.
  6. 6. The trans-2-hexenal oil-in-water nanoemulsion of claim 3, wherein when the co-emulsifier is chitosan, the raw material of the trans-2-hexenal oil-in-water nanoemulsion further comprises acetic acid.
  7. 7. The trans-2-hexenal oil-in-water nanoemulsion of claim 6, wherein the mass ratio of acetic acid to water is (0.01-0.1): 1.
  8. 8. The method for preparing the trans-2-hexenal oil-in-water nanoemulsion according to any one of claims 1-7, which is characterized by comprising the steps of mixing an emulsifier, water and trans-2-hexenal, and then performing shearing treatment to obtain the trans-2-hexenal oil-in-water nanoemulsion; When the raw materials comprise the auxiliary emulsifier and acetic acid, the emulsifier, water, trans-2-hexenal, the auxiliary emulsifier and acetic acid are mixed and then subjected to shearing treatment, so that the trans-2-hexenal oil-in-water nanoemulsion is obtained.
  9. 9. The method according to claim 8, wherein the shearing treatment is performed at a rate of 4000 to 5000rpm for a time of 4 to 10 minutes.
  10. 10. The use of the trans-2-hexenal oil-in-water nanoemulsion according to any one of claims 1-7 or the trans-2-hexenal oil-in-water nanoemulsion prepared by the preparation method of claim 8 or 9 in inhibiting sprouting of arthrodesis seed.

Description

Trans-2-hexenal oil-in-water nanoemulsion and preparation method and application thereof Technical Field The invention belongs to the technical field of biology, and particularly relates to trans-2-hexenal oil-in-water nanoemulsion and a preparation method and application thereof. Background Trans-2-hexenal (Trans-2-hexenal, T2H), also known as leaf aldehyde, belongs to typical plant source volatile organic compounds (Volatile organic compounds, VOCs), has excellent environmental compatibility, and is a natural active substance with great development potential. The wheat Tian Nan is used for controlling weeds, at present, the wheat Tian Nan is spread to at least 15 main wheat producing provinces, the wheat production safety is seriously threatened, and the wheat production reduction can be caused to be 20% -30%, and even local land mass is in harvest. Because of the similarity of physiological and biochemical characteristics of the Artemisia princeps Pampanini and the wheat, the chemical agent for selectively controlling the wheat Tian Jiejie is extremely deficient in production, and development of green compounds or green control technologies for controlling the wheat Tian Jiejie is needed. Early researches prove that the trans-2-hexenal has remarkable inhibiting effect on the germination of the arthromyodynia seeds, and has the potential of being developed as a weed germination inhibitor and directionally reducing a soil arthromyodynia seed bank. However, in the actual soil application scene, the trans-2-hexenal has core defects of too high volatilization rate and poor soil fluidity, so that the trans-2-hexenal is difficult to maintain effective concentration in the soil, and the inhibition effect on the germination of the arthromyodynia seeds is greatly weakened. Therefore, the method overcomes the physicochemical defect of the trans-2-hexenal, improves the inhibition effect of the trans-2-hexenal on the sprouting of the arthrodesis seeds, and is a technical problem to be solved in the current green prevention and control field of the grass. Based on the defects of the prior art and the actual prevention and control requirements of the industry, the invention is particularly provided. Disclosure of Invention The invention aims to provide trans-2-hexenal oil-in-water nanoemulsion and a preparation method and application thereof. The trans-2-hexenal oil-in-water nanoemulsion provided by the invention has a better inhibition effect on the sprouting of the arthromyodynia seeds. In order to achieve the above object, the present invention provides the following technical solutions: the invention provides a trans-2-hexenal oil-in-water nanoemulsion, which is prepared from the following raw materials of trans-2-hexenal, water and an emulsifier; the emulsifying agent is Tween-80 and Span-80, and the mass ratio of the Tween-80 to Span-80 is 1 (5-9); The content of the emulsifier in the trans-2-hexenal oil-in-water nanoemulsion is 0.9-25wt%, and the content of the trans-2-hexenal is 0.2-50wt%. Preferably, the raw material of the trans-2-hexenal oil-in-water nanoemulsion further comprises a co-emulsifier. Preferably, the co-emulsifier comprises sodium alginate or chitosan. Preferably, when the coemulsifier is sodium alginate, the content of sodium alginate in the trans-2-hexenal oil-in-water nanoemulsion is less than or equal to 0.05wt%. Preferably, when the co-emulsifier is chitosan, the content of chitosan in the trans-2-hexenal oil-in-water nanoemulsion is more than or equal to 0.45wt%. Preferably, when the co-emulsifier is chitosan, the raw material of the trans-2-hexenal oil-in-water nanoemulsion further comprises acetic acid. Preferably, the mass ratio of the acetic acid to the water is (0.01-0.1): 1. The invention also provides a preparation method of the trans-2-hexenal oil-in-water nanoemulsion, which comprises the steps of mixing an emulsifier, water and trans-2-hexenal, and then performing shearing treatment to obtain the trans-2-hexenal oil-in-water nanoemulsion; When the raw materials comprise the auxiliary emulsifier and acetic acid, the emulsifier, water, trans-2-hexenal, the auxiliary emulsifier and acetic acid are mixed and then subjected to shearing treatment, so that the trans-2-hexenal oil-in-water nanoemulsion is obtained. Preferably, the speed of the shearing treatment is 4000-5000 rpm, and the time of the shearing treatment is 4-10 min. The invention also provides an application of the trans-2-hexenal oil-in-water nanoemulsion in the technical scheme or the trans-2-hexenal oil-in-water nanoemulsion prepared by the preparation method in the technical scheme in inhibiting the sprouting of the arthrodesis seed. The invention provides a trans-2-hexenal oil-in-water nanoemulsion, which is prepared from the following raw materials of trans-2-hexenal, water and an emulsifying agent, wherein the emulsifying agent is Tween-80 and Span-80, the mass ratio of the Tween-80 to the Span-80 is 1 (5-9), the co