CN-121986785-A - Aphid pest attractant
Abstract
The invention provides an aphid insect attractant, which uses benzyl isobutyrate as a myzus persicae attractant. The invention discovers that the phenylmethyl isobutyrate can be specifically combined with the myzus persicae smell binding protein on the molecular level, thereby being used as an effective active ingredient of the myzus persicae attractant. The benzyl isobutyrate is used as an active component of the attractant, so that the trending response to the myzus persicae can be obviously enhanced, and the attracting efficiency can be improved. Meanwhile, the compound has definite structure and controllable source, is convenient for large-scale preparation and application, is favorable for reducing the use amount of chemical pesticides and reducing the environmental pollution risk, is suitable for green prevention and control of myzus persicae, and has good application prospect.
Inventors
- LIU JIAHUI
- REN GUANGWEI
- XU PENGJUN
- SU CHENYU
- WANG XIUFANG
- WANG YING
Assignees
- 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所)
Dates
- Publication Date
- 20260508
- Application Date
- 20260113
Claims (6)
- 1. The application of benzyl isobutyrate as a myzus persicae attractant.
- 2. An aphid pest attractant, which is characterized in that, the aphid pest attractant comprises benzyl isobutyrate.
- 3. An aphid pest attractant according to claim 2, wherein the attractant is a benzyl isobutyrate in ethyl acetate.
- 4. A method for attracting myzus persicae, which is characterized in that benzyl isobutyrate is used for attracting myzus persicae.
- 5. The method of claim 4, wherein the benzyl isobutyrate is used at a concentration of 0.001 to 10 μg/mL.
- 6. The method of claim 5, wherein the benzyl isobutyrate is used at an optimal concentration of 1 μg/mL.
Description
Aphid pest attractant Technical Field The invention belongs to the technical field of green prevention and control of plant insect pests, and particularly relates to an aphid insect pest attractant. Background Myzus persicae (Myzus persicae), also known as Myzus persicae, is an important agricultural pest distributed globally, has extremely wide host range, belongs to typical multi-feeding insects, and can harm more than 50 families and more than 400 plants. The myzus persicae sucks plant sap through the adult aphids and the myzus persicae, resulting in stunted plant growth, leaf curl and malformation. In addition, myzus persicae is an important transmission medium for various plant viruses, and it is known that more than hundred plant viruses can be transmitted, which poses a serious threat to crop yield and quality. At present, the aphid control is mainly chemical pesticides, and the aphid resistance is easy to cause for long-term application, and pesticide residues and ecological environment pollution can be caused. The physical and chemical control technology is an important green control strategy for controlling pests, and the fuming aphid attractant can provide support for the green control technology and is beneficial to reducing the using amount of chemical pesticides. Disclosure of Invention The invention aims to provide an aphid pest attractant, so as to make up for the defects of the prior art. The invention firstly provides an application of benzyl isobutyrate, which is an application as a myzus persicae attractant; In another aspect, the invention provides an aphid pest attractant comprising benzyl isobutyrate. Further, the aphid pest attractant is ethyl acetate solution of benzyl isobutyrate. The invention also provides a method for attracting the myzus persicae, which uses the benzyl isobutyrate to attract the myzus persicae; The benzyl isobutyrate is used at a concentration of 0.001-10. Mu.g/mL, preferably 1. Mu.g/mL. The invention discovers that the phenylmethyl isobutyrate can be specifically combined with the myzus persicae smell binding protein on the molecular level, thereby being used as an effective active ingredient of the myzus persicae attractant. The benzyl isobutyrate is used as an active component of the attractant, so that the trending response to the myzus persicae can be obviously enhanced, and the attracting efficiency can be improved. Meanwhile, the compound has definite structure and controllable source, is convenient for large-scale preparation and application, is favorable for reducing the use amount of chemical pesticides and reducing the environmental pollution risk, is suitable for green prevention and control of myzus persicae, and has good application prospect. Drawings Figure 1 is a graph showing the effect of benzyl isobutyrate on the attraction of myzus persicae, FIG. 2 is a graph showing the results of principal component analysis of tobacco and other host plant volatiles, FIG. 3 is a graph showing the comparison of the levels of key volatile components in tobacco and other host plants. FIG. 4 shows the olfactory behavior response of Aphis tabaci to candidate compounds, FIG. 5 shows the olfactory behavior of Aphis tabaci against benzyl isobutyrate of different concentrations, FIG. 6 is a graph showing the results of binding of the Aphis tabacum odor binding protein to the fluorescent probe (A) and benzyl isobutyrate (B). Detailed Description During experimental investigation it was found that myzus persicae showed different preferences for different host plants. Compared with cabbage, rape, capsicum and peach, myzus persicae has a strong preference for tobacco. By analyzing the tobacco volatile, the benzyl isobutyrate is identified as one of the key components in the tobacco volatile. Further behavioral test results show that the phenylmethyl isobutyrate has remarkable attraction effect on the myzus persicae. Based on the above findings, benzyl isobutyrate can be used as an effective active ingredient for preparing a myzus persicae attractant. The present invention will be described in detail with reference to the following examples and the accompanying drawings. Example 1 determination of the efficacy of benzyl isobutyrate in attracting Aphis tabaci 1. Behavioural responses of myzus persicae to different host plants And (3) measuring the selective behaviors of the myzus persicae on different host plants by adopting a Y-type olfactometer. The host plants are respectively placed in two customized glass containers, wherein one side is tobacco, and the other side is cabbage, rape, chilli or peach. The Y-shaped olfactometer is arranged in a camera bellows, and a light source is arranged above the camera bellows, wherein the illumination intensity is 100 lx so as to eliminate the interference of illumination factors on the olfactory behaviors of the myzus persicae. The air flow rates of the two arms of the Y-shaped tube are controlled by the flowmeter to be 150 mL/mi