CN-121986787-A - Application of N-lauroyl isoleucine in improving rice sheath blight resistance

Abstract

The invention discloses an application of N-lauroyl isoleucine in improving rice sheath blight resistance, relating to the technical field of plant immunity inducer and green agricultural input products, systemic resistance of rice to banded sclerotial blight can be induced by activating the OsNPR1-OsPR2-OsMAPK6 pathway, and therefore N-lauroyl isoleucine can be used as an active ingredient of a plant immunity inducer. The plant immunity inducer prepared by taking the N-lauroyl isoleucine as the active ingredient can be controlled by a spraying method, has strong operability, is simple and quick, and can effectively control the spread of diseases. In addition, the N-lauroyl isoleucine is a biological endogenous metabolite, is easy to degrade and has no residual risk, and the N-lauroyl isoleucine is low in cost, thereby conforming to the current sustainable development direction of agriculture.

Inventors

• ZHANG DEYONG
• KANG HOUXIANG
• SU PIN
• PENG WEIYE
• Peng Qianze
• GAO LIMING

Assignees

• 岳麓山实验室

Dates

Publication Date

20260508

Application Date

20260115

Claims (10)

1. Use of n-lauroyl isoleucine for increasing rice sheath blight resistance.
2. 2. The use according to claim 1, wherein said N-lauroyl isoleucine induces systemic resistance of rice to banded sclerotial blight by activating the osppr 1-OsPR2-OsMAPK pathway.
3. Use of n-lauroyl isoleucine in the preparation of a plant immune elicitor.
4. 4. A plant immune elicitor comprising a live active ingredient and an adjunct ingredient, wherein the active ingredient comprises at least 5-20 mu M N-lauroyl isoleucine.
5. 5. The plant immunity-inducing agent according to claim 4, wherein the active ingredient further comprises a trehalose.
6. 6. The plant immunity-inducing agent according to claim 4, wherein the auxiliary components include a solvent, a dispersant, a stabilizer and a synergist.
7. 7. The plant immunity-inducing antibody according to claim 6, wherein the solvent is DMSO and the dispersant is a surfactant.
8. 8. The plant immunity-inducing antibody according to claim 7, wherein the surfactant is tween 20, and the tween 20 accounts for 0.01-0.04% by mass.
9. 9. The plant immune elicitor according to claim 4, wherein the plant immune elicitor is in the form of a suspension or microcapsule suspension.
10. 10. A method for improving rice sheath blight resistance, which comprises spraying the plant immunity-inducing agent according to any one of claims 4 to 9 onto the leaf or root part of rice at the tillering stage and/or the breaking stage of the rice.

Description

Application of N-lauroyl isoleucine in improving rice sheath blight resistance Technical Field The invention belongs to the field of plant immunity elicitors and green agricultural input products, relates to a novel application of lipid-amino acid conjugate N-lauroyl isoleucine, in particular to a novel application of N-lauroyl isoleucine low-concentration foliar spray on rice NPR1-PR2-MAPK6 defense paths, which can obviously inhibit sheath blight bacterial plaque expansion, and can replace or reduce chemical bactericides. Background The rice sheath blight disease is a fungal disease caused by the infection of rhizoctonia solani (Rhizoctonia solani, R.solani), the pathogenic bacteria belong to the phylum of the half-known mycotina, the behaviour is the leather melon dying fungus (Thanatephorus cucumeris), and the pathogenic bacteria silk fusion group is mainly AG-1. The disease mainly damages leaf sheath and leaf blade, and forms dark green water stain-shaped elliptic disease spots on the leaf sheath near the water surface in the early stage of disease, gradually expands into moire shape, and generates white hyphae and dark brown sclerotium when the humidity is high. The onset peak is usually from booting to heading, the temperature is 22-28 ℃ and the high humidity environment is easy to prevail. The rice sheath blight disease causes 10% -30% of yield loss throughout the year, and the loss of the serious disease field can reach more than 50%, so that the disease becomes the primary disease affecting the high and stable yield of rice in China. The control adopts agricultural measures combined with medicament control, and the chemical medicament can be validamycin, thiophanate-methyl, hexaconazole, thifluzamide and the like, and is mainly sprayed to the middle and lower parts of plants. The prevention and control method through the chemical agent is easy to induce drug resistance and has high ecological risk, and the long-term massive use of the chemical bactericide leads to the annual increase of the drug resistance population frequency of pathogenic bacteria, and simultaneously causes unbalance of soil microbial communities and environmental pollution of water. Plant immunity elicitors can activate Systemic Acquired Resistance (SAR), but have the bottlenecks of high cost, short duration, unstable field effect, and the like. Therefore, there is still an urgent need in the industry to develop low concentration, low cost, easily degradable, environmentally friendly small molecule elicitors. N-lauroyl isoleucine (N-Lauroyl Isoleucine, CAS number: 56599-38-9) is an N-acyl amino acid compound, which is formed by condensing lauric acid (C12 saturated fatty acid) and isoleucine through an amide bond, and has a molecular formula of C18H35NO3 and a molecular weight of 313.47 g/mol. The compounds are widely distributed in nature, can be prepared by enzymatic or chemical derivatization of natural oil (such as coconut oil and palm kernel oil), and can also be obtained by microbial fermentation. N-lauroyl isoleucine has good surface activity, antibacterial and anti-inflammatory properties, and has been used as a mild surfactant and a functional additive in the field of daily chemicals. In recent years, research shows that the N-acyl amino acid compound has unique bioactivity in aspects of cell signal transduction, membrane permeability regulation and the like, and the combination of a long-chain acyl structure and an amino acid polar group of the N-acyl amino acid compound endows the N-acyl amino acid compound with amphiphilicity characteristics, so that the N-acyl amino acid compound is easy to interact with a biological membrane and trigger downstream response. However, the role of N-lauroyl isoleucine in plant immune regulation has not been reported, and the activation mechanism, the effective concentration range and the field application potential of the N-lauroyl isoleucine to the rice defense system are not clear. In view of the fact that the compound can be derived from natural oil and fat, has good biodegradability, low preparation cost and excellent environmental compatibility, the invention provides application of N-lauroyl isoleucine in improving rice sheath blight resistance, and aims to develop a novel plant immunity inducer based on the small molecular compound, thereby providing a novel approach for green prevention and control of rice sheath blight. Disclosure of Invention Aiming at the defects existing in the prior art, the invention aims to provide the application of N-lauroyl isoleucine in improving the rice sheath blight resistance, and the small molecule inducer prepared by adopting the N-lauroyl isoleucine is used for improving the rice sheath blight resistance. In order to achieve the above purpose, the technical scheme of the invention is as follows: in a first aspect, the present invention provides the use of N-lauroyl isoleucine for increasing rice sheath blight resistance. Preferably, the long