CN-121986788-A - Application of N-stearoyl glycine in improving rice blast resistance

CN121986788ACN 121986788 ACN121986788 ACN 121986788ACN-121986788-A

Abstract

The invention discloses an application of N-stearoyl glycine in improving rice blast resistance, relating to the technical field of plant immunity inducer and green agricultural input products, systemic resistance of rice to rice blast can be induced by activating the OsNPR1-OsPR2-OsMAPK module, and therefore, N-stearoyl glycine can be used as an effective ingredient for plant immunity resistance. The plant immunity inducer prepared by taking the N-stearoyl glycine as an active ingredient can be controlled by a spraying method, has strong operability, is simple and quick, and can effectively control the spread of diseases. In addition, the N-stearoyl glycine is an animal endogenous metabolite, is easy to degrade and free of residual risks, has low cost, and accords with the current sustainable development direction of agriculture.

Inventors

Peng Qianze
KANG HOUXIANG
ZHANG DEYONG
SU PIN
PENG WEIYE
GAO LIMING

Assignees

岳麓山实验室

Dates

Publication Date: 20260508
Application Date: 20260115

Claims (9)

Use of n-stearoyl glycine for increasing the resistance to rice blast.
2. The use according to claim 1, wherein the N-stearylglycine increases rice blast resistance by up-regulating the expression of osppr 1, osPR2 and OsMAPK.
Use of n-stearoyl glycine in the preparation of a plant immune elicitor.
4. A plant immune elicitor comprising an active ingredient and an adjunct ingredient, the active ingredient comprising at least 5-20 μ M N-stearoyl glycine.
5. The plant immune elicitor according to claim 4, wherein the active ingredient further comprises a trehalose or a chitosan oligosaccharide.
6. The plant immunity-inducing agent according to claim 4, wherein the auxiliary components include a solvent, a dispersant, a stabilizer and a synergist.
7. The plant immune elicitor according to claim 4, wherein the solvent is DMSO.
8. The plant immunity-inducing antibody according to claim 4, wherein the dispersant is tween 20, and the tween 20 accounts for 0.01-0.04% by mass.
9. A method for improving rice blast resistance, which comprises spraying the plant immunity-inducing agent according to any one of claims 4-8 onto the leaf or root part of rice in the trefoil-core stage, and/or tillering stage, and/or break-out stage of the rice.

Description

Application of N-stearoyl glycine in improving rice blast resistance Technical Field The invention belongs to the field of plant immunity elicitors and green agricultural input products, relates to a novel application of an endogenous lipid amino acid conjugate N-stearoyl glycine, and particularly relates to an application of N-stearoyl glycine in activating rice system acquired resistance by a leaf surface spraying mode, obviously reducing rice blast bacterial biomass and replacing or reducing chemical pesticide use. Background The rice blast is a fungal disease of rice caused by ascomycete rice blast fungus (Magnaporthe oryzae, M. Oryzae), which is used as a primary limiting factor for global rice production, and causes 10-20% of rice yield reduction in each year, 40-50% of epidemic year loss, and no granule harvest in serious cases. The pathogenic bacteria are attached to the surface of rice tissues through conidium, germinate to form attached cells and generate huge mechanical pressure to penetrate through epidermis, so that secondary hyphae are formed in host cells, and each tissue organ in the whole growth period of the rice is systematically infected, wherein the tissue organs comprise seedling plague, leaf plague, festival plague and spike plague. In recent years, due to global warming, single genetic diversity of rice varieties and changing cultivation system, the physiological race variation rate of rice blast bacteria is accelerated, the period of the resistance loss of the traditional disease-resistant varieties is shortened to 3-5 years, the annual average disease area of Yangtze river basin and northeast rice area exceeds 800 ten thousand hectares, and the direct economic loss is over 30 hundred million yuan. The current production mainly depends on chemical bactericide control, and triazole (such as tebuconazole and difenoconazole), succinic acid dehydrogenase inhibitor (such as prochloraz and fluoroamide) and methoxy acrylic ester (such as azoxystrobin) are mainly used. However, long-term repeated use has led to the detection of drug-resistant strains in rice areas such as the Heilongjiang river, jiangsu, etc., where the azoxystrobin resistance index has risen 8-15 times. The chemical control not only aggravates the drug resistance evolution of pathogenic bacteria, but also brings more serious food safety and ecological risks that the detection rate of triazole pesticides in rice in 2022 is up to 17.3 percent and the residual exceeding rate is 4.2 percent, and the toxic effect of the bactericide on non-target organisms such as diatom and green algae in rice fields damages the aquatic microecological balance, so that natural enemy insect population is degenerated. From the economic aspect, the annual average cost of chemical control reaches 600-800 yuan/hectare, and the cost of manpower and medicament accounts for more than 60 percent of the total expenditure of rice pest control, which severely restricts the green sustainable development of agriculture. The plant immunity inducer activates host systemic resistance (SAR) through simulating pathogen-associated molecular patterns (PAMPs), and has the advantages of broad spectrum, lasting effect, environmental friendliness and the like. The representative products of Benzothiadiazole (BTH) and chitooligosaccharide are applied in partial areas, but have obvious technical bottlenecks that the effective concentration of BTH needs 200-400 mu M, the raw material cost is high (market price is about 8000 yuan/kg), rice leaf premature senility and yield loss are easy to occur at high concentration, the duration of oligosaccharide inducer is only 7-10 days, the labor cost is increased by frequent application, and in addition, the existing inducer has single action mechanism and the adaptive evolution risk of pathogenic bacteria exists when the existing inducer is applied for a long time. Therefore, development of novel small molecule inducers with low cost, low concentration, multiple targets and easy degradation has become an urgent need of the green prevention and control industry of rice. N-stearoyl glycine (N-Stearoyl glycine) is a natural fatty amino acid compound of formula C 20H39NO3, which can be synthesized in vivo in animals from stearic acid and glycine via the N-acyltransferase pathway. The prior medical research shows that the substance has potential anti-inflammatory activity and glycolipid metabolism regulating function, LD 50 is more than 2000 mg/kg, half life period is 2.8 hours, and metabolite is natural fatty acid and amino acid. However, the physiological function of N-stearoyl glycine in plants is completely unknown, and whether it can activate innate immunity of rice and induce resistance to rice blast as an exogenous inducer has not been reported so far. In view of the characteristics of low toxicity, easy degradation and low cost (about 150 yuan/kg of synthetic raw materials), the N-stearoyl glycine has remarkable theoretical inno