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CN-121986789-A - Plant growth-promoting stress-resistant agent of micromolecular collagen active peptide, and preparation method and application thereof

CN121986789ACN 121986789 ACN121986789 ACN 121986789ACN-121986789-A

Abstract

The invention provides a plant growth-promoting stress-resisting agent of small molecular collagen active peptide, a preparation method and application thereof, wherein the plant growth-promoting stress-resisting agent comprises, by weight, 5-10% of small molecular collagen active peptide, 4-8% of soybean peptide-zinc chelate, 3-12% of mineral elements, 2-5% of seaweed extract, 1-3% of chitosan oligosaccharide and the balance of deionized water. The growth-promoting stress-resistant agent realizes the integrated functions of nutrition supply, high-efficiency delivery of trace elements and stress-resistant protection through multi-component cooperation, can obviously improve crop yield and enhance stress resistance, is environment-friendly, has simple and convenient process, and is suitable for various crop and stress planting scenes.

Inventors

  • WU KEXIANG
  • WU HELING
  • Chang Laohu

Assignees

  • 河南沃普丰肥业有限公司

Dates

Publication Date
20260508
Application Date
20260306

Claims (10)

  1. 1. The plant growth promoting stress-resistant agent is characterized by comprising, by weight, 5-10% of small molecular collagen active peptide, 4-8% of soybean peptide-zinc chelate, 3-12% of mineral elements, 2-5% of seaweed extract, 1-3% of chitosan oligosaccharide and the balance of deionized water.
  2. 2. The plant growth-promoting stress-resistant agent of small molecular collagen active peptide according to claim 1, wherein the molecular weight of the small molecular collagen active peptide is 800-2000Da, and the small molecular collagen active peptide is derived from fish scales and fish skins.
  3. 3. The plant growth-promoting stress-resistant agent of small molecular collagen active peptide according to claim 2, wherein the soybean peptide-zinc chelate is prepared by denaturation, enzymolysis and zinc ion chelation of soybean protein isolate.
  4. 4. A plant growth-promoting stress-relieving agent of small molecular collagen active peptide according to claim 3, wherein said mineral element is selected from one or more of boron element, calcium element, iron element and manganese element.
  5. 5. The plant growth-promoting stress-resistant agent of small molecular collagen active peptide according to claim 4, wherein the molecular weight of the chitosan oligosaccharide is 500-2000Da, and the alginic acid content in the seaweed extract is more than or equal to 30% and the mannitol content is more than or equal to 15%.
  6. 6. The plant growth-promoting stress-resistant agent of small molecular collagen active peptide according to claim 5, wherein the preparation of the soybean peptide-zinc chelate comprises the following steps: S1, adding soybean protein isolate into deionized water to prepare soybean protein isolate solution with the mass fraction of 10%, dispersing the solution into 0.1mol/L Tris-HCl buffer solution, magnetically stirring for 25-30min until the protein is completely dissolved to form uniform dispersion, placing the dispersion into a constant temperature water bath kettle with the temperature of 45-55 ℃, preserving heat and reducing for 1.0-1.5h, heating the reduced protein solution to 95 ℃ in a water bath, preserving heat for 8-10min to strengthen denaturation, and then rapidly cooling to 40-45 ℃ to obtain pretreated protein solution; S2, adding a calcium hydroxide solution into the pretreated protein solution, regulating the pH of the system to 8.0-8.5, adding alkaline protease, carrying out oscillating enzymolysis for 20-25min at 40-45 ℃ and 150-200r/min, continuously adding the calcium hydroxide solution, regulating the pH of the system to 7.0-7.5, adding neutral protease, adding zinc sulfate powder, stirring while adding 200r/min, and maintaining the reaction at 42-45 ℃ for 45-50min to obtain an enzymolysis-chelation mixed solution; S3, after the reaction is finished, quickly heating the enzymolysis-chelation mixed solution to 90-95 ℃, preserving heat for 8-10min to inactivate protease, cooling to room temperature, introducing the cooled mixed solution into a disc centrifuge, centrifuging for 10min at 5000r/min, discarding the bottom non-enzymolysis protein precipitate, and collecting supernatant, wherein the supernatant is filtered by a ceramic microfiltration membrane of 0.22-0.25 mu m to remove trace suspended matters and macromolecular impurities, thus obtaining clear filtrate; S4, slowly adding absolute ethyl alcohol into the clear filtrate, stirring uniformly, standing for 1.0-1.5h in a cold storage at 2-6 ℃ to promote the aggregation of chelate, introducing a precipitation system into a centrifugal machine, centrifuging for 8-12min at 4800-5200r/min, removing supernatant containing free zinc ions and micromolecular impurities, collecting bottom precipitate, washing the precipitate with absolute ethyl alcohol for 1-2 times, centrifuging and collecting after washing each time, transferring the washed precipitate into a vacuum freeze dryer, and drying for 22-26h to constant weight to obtain the soybean peptide-zinc chelate.
  7. 7. The plant growth-promoting stress-resistant agent of small molecular collagen active peptide according to claim 6, wherein the addition amount of the zinc sulfate powder is 240-400% of the mass of the isolated soybean protein.
  8. 8. The plant growth-promoting stress-resistant agent of small molecular collagen active peptide according to claim 7, wherein the addition amount of the alkaline protease is 1-1.5% of the mass of the isolated soybean protein, and the enzyme activity of the alkaline protease is more than or equal to 20 ten thousand U/g; the addition amount of the neutral protease is 2-3.5% of the mass of the soybean protein isolate, and the enzyme activity of the neutral protease is more than or equal to 10 ten thousand U/g; The mass fraction of the calcium hydroxide solution is 8-12%.
  9. 9. The method for preparing the plant growth-promoting stress-resistant agent of the small molecular collagen active peptide according to claim 8, which is characterized by comprising the following steps: (1) Diluting the micromolecular collagen active peptide to 10-15% by deionized water, regulating the pH to 6.5-8.5, heating to 35-45 ℃, adding boric acid to react for 1-1.5h, adding calcium nitrate to react for 30-40min, sequentially adding various mineral elements to react for 0.5 h, and keeping the stirring speed at 150-200r/min during the period to obtain the micromolecular collagen active peptide chelating solution; (2) And maintaining the temperature at 30-40 ℃, adding soybean peptide-zinc chelate, seaweed extract and chitosan oligosaccharide into the small molecular collagen active peptide chelating solution, stirring and reacting for 1-1.5 hours, adjusting the pH to 4-5, standing and precipitating for 1.5-2.5 hours, and filtering to obtain the plant growth-promoting stress-resistant agent of the small molecular collagen active peptide.
  10. 10. The application of the plant stress-accelerating agent of the small molecular collagen active peptide according to any one of claims 1 to 8, which is characterized in that the stress-accelerating agent is sprayed on the leaf surfaces of crops in a dilution of 800-1500 times or irrigated with roots in a dilution of 500-800 times, and is suitable for crops including wheat, corn, rice, celery, tomatoes, cucumbers, apples, oranges or strawberries.

Description

Plant growth-promoting stress-resistant agent of micromolecular collagen active peptide, and preparation method and application thereof Technical Field The invention relates to the technical field of agricultural biology, in particular to a plant growth-promoting stress-resisting agent of a small molecular collagen active peptide, a preparation method and application thereof. Background Along with the transformation of agriculture to green and high quality, the traditional plant nutrient has hardly met the comprehensive requirements of quality improvement, yield increase, stress resistance and ecological friendliness. In the prior art, a scheme of preparing small molecular collagen peptide by utilizing fishery waste is adopted as a plant nutrient, such as CN101481266B, a plant nutrient is adopted, the fishery processing waste (fish skin, fish scales, fish bones and the like) is taken as a raw material, the small molecular collagen peptide is prepared by degradation through a complex enzyme enzymolysis process, and is directly used as the plant nutrient/foliar fertilizer/flush fertilizer, although nutrition supply and yield improvement are realized, obvious limitations are that firstly, the growth promotion and stress resistance functions are insufficient, the plant immunity can be only improved to indirectly prevent diseases, the drought, the saline-alkali and other abiotic stresses are lack of pertinence protection, secondly, the delivery efficiency of trace elements is low, the traditional zinc source (such as zinc sulfate) is easy to precipitate in a weak alkaline environment, the absorption and the utilization rate of plant rhizosphere is insufficient, antagonism exists with other elements, and thirdly, the preparation ingredient is single, the synergistic effect of bioactive substances is not fully utilized, and the overall performance is limited. Meanwhile, the existing zinc-supplementing plant nutrient adopts a mode of directly adding inorganic zinc salt, and has the problems of poor stability, easiness in being fixed by soil, low bioavailability and the like. Although some technologies try to adopt peptide-zinc chelate, the preparation process is complex, the chelation rate is low, and the peptide-zinc chelate is not effectively combined with other growth-promoting stress-resistant components, so that the integrated functions of nutrition supply, high-efficiency delivery and stress-resistant protection cannot be realized. In addition, the stress-resistant components such as seaweed extract, chitosan oligosaccharide and the like are mostly simply compounded in the existing preparation, a synergistic system with nutritional components is not formed, and the stress-resistant effect is greatly reduced. Therefore, the development of a plant preparation with synergistic components, high trace element utilization rate and double effects of growth promotion and stress resistance becomes a key for solving the pain point of the current green agricultural production. Disclosure of Invention The invention aims to: The invention aims to overcome the defects of single growth promoting and stress resisting functions, low trace element utilization rate and poor environmental adaptability of the existing plant nutrient, and provides the plant growth promoting and stress resisting agent of the micromolecular collagen active peptide, which can provide full nutrition for plants, realize high-efficiency and stable delivery of zinc element, enhance the resistance of plants to drought, salt and alkali, diseases and insect pests and the like, and is nontoxic, residue-free and capable of improving soil through the multi-component synergistic effect; meanwhile, the preparation method which is simple in process and can be realized industrially and wide in agricultural application scene are provided. The technical scheme of the invention is as follows: The invention relates to a plant growth-promoting stress-resisting agent of small molecular collagen active peptide, which comprises, by weight, 5-10% of small molecular collagen active peptide, 4-8% of soybean peptide-zinc chelate, 3-12% of mineral elements, 2-5% of seaweed extract, 1-3% of chitosan oligosaccharide and the balance of deionized water. Further, the molecular weight of the small molecular collagen active peptide is 800-2000Da, and the small molecular collagen active peptide is derived from fish scales and fish skin. Further, the soybean peptide-zinc chelate is prepared by denaturation, enzymolysis and zinc ion chelation of soybean protein isolate. Further, the mineral element is selected from one or more of boron element, calcium element, iron element and manganese element. Further, the molecular weight of the chitosan oligosaccharide is 500-2000Da, and the alginic acid content in the seaweed extract is more than or equal to 30% and the mannitol content is more than or equal to 15%. Further, the preparation of the soybean peptide-zinc chelate comprises the followi