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CN-121986801-A - Bacillus bailii dispersible oil suspending agent and application thereof

CN121986801ACN 121986801 ACN121986801 ACN 121986801ACN-121986801-A

Abstract

The invention discloses a bacillus bailii dispersible oil suspending agent. The dispersible oil suspending agent comprises, by mass, 1% -5% of bacillus beijerinus BJ-1 parent drug, 16% -20% of an emulsifying agent, 8% -12% of a wetting dispersing agent, 1% -2% of a thickening agent, 1% -2% of a stabilizing agent and the balance peanut oil or soybean oil. The normal temperature storage result shows that the preparation can raise the live bacteria rate of the mother medicine by more than 10% when stored for 6 months. According to the bacillus bailii dispersible oil suspending agent, through normal storage screening of different auxiliary agents and dispersion media, the auxiliary agents or dispersion media with good biocompatibility on a parent drug are selected, so that the viable bacteria rate of bacillus bailii during normal-temperature storage can be improved, the duration of active ingredients can be prolonged, and the control effect of the bacillus bailii dispersible oil suspending agent can be improved. The indoor potting results show that the relative control effect of the indoor potting results on the gray mold of the tomatoes is 78% -88%. In addition, the bacillus beijerinus dispersible oil suspending agent has good control effect on tomato fusarium wilt and cucumber powdery mildew, and can promote crop growth and increase the income of growers.

Inventors

  • HAN PING
  • WU JUNXUE
  • ZHANG WANQIANG
  • ZHANG WEI
  • YAN JIYE

Assignees

  • 北京市农林科学院

Dates

Publication Date
20260508
Application Date
20260125

Claims (7)

  1. 1. The bacillus bailii dispersible oil suspending agent consists of the following components in percentage by mass: 1 to 5 percent of bacillus bailii parent drug, 16-20% Of emulsifying agent, 8-12% Of wetting dispersant, 1-2% Of thickener, 1% -2% Of stabilizer, A dispersion medium, the balance; the bacillus bailii parent drug is bacillus bailii (Bacillus velezensis) BJ-1 bacterial powder, and the effective viable count is 1.0X10 11 ～1.5×10 11 cfu/g; the emulsifier is one or a combination of EL-10 and Tween-80, the wetting dispersant is PEG400, the thickener is one or a combination of SK04 and CMC-Na, the stabilizer is dextrin, and the dispersion medium is peanut oil or soybean oil.
  2. 2. The bacillus bailii dispersible oil suspension of claim 1 wherein bacillus bailii (Bacillus velezensis) BJ-1 has been stored in the chinese microbiological culture collection center under the accession number CGMCC No.24113.
  3. 3. The bacillus bailii dispersible oil suspension of claim 1, wherein the emulsifier is a combination of EL-10 and Tween-80, wherein EL-10 accounts for 80% -90% of the total mass of the emulsifier, tween-80 accounts for 10% -20%, the wetting dispersant is PEG400, the thickener is a combination of SK04 and CMC-Na, wherein SK04 accounts for 75% -90% of the total mass of the thickener, CMC-Na accounts for 10% -25%, the stabilizer is dextrin, and the dispersion medium is peanut oil or soybean oil.
  4. 4. The bacillus bailii dispersible oil suspension agent according to claim 1 is characterized by comprising, by mass, 1% -5% of bacillus bailii parent drug, 15% -17% of EL-10, 1% -3% of Tween-80, 8% -12% of PEG400, 0.5% -1.5% of SK04, 0.1% -0.5% of CMC-Na, 1% -2% of dextrin and the balance of dispersion medium, wherein the dispersion medium is peanut oil or soybean oil.
  5. 5. The method for preparing the bacillus bailii dispersible oil suspension of any one of claims 1 to 4, comprising the steps of: 1) Weighing the components according to the content of the components; 2) Mixing the materials except bacillus bailii parent drug, adding grinding medium to grind by wet method to make the grain diameter D50 less than or equal to 1.5 μm and D90 less than or equal to 3 μm; 3) And after the grinding medium is removed, adding bacillus bailii parent drug into the obtained system, and stirring and mixing uniformly to obtain bacillus bailii dispersible oil suspending agent.
  6. 6. Use of the bacillus bailii dispersible oil suspension of any one of claims 1-4 for controlling plant diseases and/or for promoting plant growth.
  7. 7. The use according to claim 6, wherein the plant disease comprises one or more of crop gray mold, wilt or powdery mildew, the crop comprises tomatoes or cucumbers, the application mode is foliar spray or irrigation, the application dosage per mu is 200-600mL, and the use is diluted 100-500 times.

Description

Bacillus bailii dispersible oil suspending agent and application thereof Technical Field The invention relates to bacillus bailii dispersible oil suspending agent and application thereof. Background The bacillus belicus (Bacillus velezensis) can produce various enzymes and antibiotics, has broad-spectrum antibacterial activity, can be used for preventing and treating gray mold, fusarium wilt and powdery mildew of crops, and simultaneously can promote the absorption of minerals by plants to generate plant growth-related hormone, and has better growth promoting effect on the plants. The existing bacillus bailii preparation market mainly uses wettable powder, but has the problems of poor dosage form quality and low spore survival rate in the processing and storage processes. The dispersible oil suspending agent has the outstanding advantages of environmental friendliness, good wetting spreadability, strong anti-scouring capability, obvious synergistic effect and the like. Bacillus belicus is encapsulated in oil phase medium to improve its duration and prolong its shelf life. Mineral oils have poor environmental compatibility and may affect the balance of soil microorganisms. While vegetable oils are more environmentally friendly, free fatty acids contained therein may accelerate degradation of bacillus. Therefore, it is important to select a vegetable oil with suitable biocompatibility as a dispersion medium for the bacillus bailii dispersible oil suspension agent. Disclosure of Invention Based on the above, the invention provides a bacillus bailii dispersible oil suspension and a preparation method thereof. The bacillus bailii dispersible oil suspending agent provided by the invention comprises the following components in percentage by mass: 1 to 5 percent of bacillus bailii parent drug, 16-20% Of emulsifying agent, 8-12% Of wetting dispersant, 1-2% Of thickener, 1% -2% Of stabilizer, The balance of dispersion medium, wherein the dispersion medium is peanut oil or soybean oil. The bacillus bailii parent drug is bacillus bailii (Bacillus velezensis) BJ-1 bacterial powder, and the effective viable count is 1.0X10 11～1.5×1011 cfu/g. The bacillus bailii (Bacillus velezensis) BJ-1 is preserved in China general microbiological culture Collection center with the preservation number of CGMCC No.24113. The emulsifier is one or more selected from EL-10, tween-80, AEO-3, NP-4, OP-10, 6501 and Span-80, the wetting dispersant is PEG400, and the thickener is one or more selected from N20st, SK04 and CMC-Na. The stabilizer is one of BHT, dextrin and ascorbic acid, and the dispersing medium is peanut oil or soybean oil. Preferably, the emulsifier is a combination of EL-10 and Tween-80, wherein EL-10 accounts for 80% -90% of the total mass of the emulsifier, tween-80 accounts for 10% -20%, the thickener is a combination of SK04 and CMC-Na, wherein SK04 accounts for 75% -90% of the total mass of the thickener, CMC-Na accounts for 10% -25%, the stabilizer is dextrin, and the dispersion medium is peanut oil or soybean oil. Preferably, the bacillus beliensis dispersible oil suspending agent comprises, by mass, 1% -5% of bacillus beliensis parent drug, 15% -17% of EL-10, 1% -3% of Tween-80, 8% -12% of PEG400, 0.5% -1.5% of SK04, 0.1% -0.5% of CMC-Na, 1% -2% of dextrin and the balance peanut oil or soybean oil. More preferably, the bacillus belicus dispersible oil suspending agent comprises, by mass, 5% of bacillus belicus parent drug, 15% -17% of EL-10, 1% -3% of Tween-80, 10% of PEG400, 1.5% of SK04, 0.5% of CMC-Na, 1% -2% of dextrin and the balance peanut oil or soybean oil. According to the bacillus bailii dispersible oil suspending agent provided by the invention, the biocompatibility of different vegetable oils is screened, the preferable sequence of the dispersing medium is determined to be peanut oil > soybean oil > sunflower oil > rapeseed oil, the living bacterial rate of bacillus bailii in peanut oil reaches 87.34 +/-3.79% according to the result of normal-temperature storage test, the living bacterial rate of bacillus bailii in soybean oil is 86.1+/-4.96% which is obviously higher than that of sunflower oil (84.64 +/-3.39%) and rapeseed oil (75.71 +/-3.83%), and the activity of effective components can be protected to the greatest extent. Although sunflower oil and rapeseed oil have certain feasibility, the retention rate of viable bacteria is lower than that of peanut oil and soybean oil, and the method is not used as a preferred embodiment. The emulsifier is compounded by adopting EL-10 and Tween-80, wherein the EL-10 mainly plays an emulsification role, the influence of the EL-10 on the water dispersion behavior of the preparation is mainly represented by the synergistic effect of the EL-80, the emulsion droplet particle size D 50 after emulsification is only 1.010 mu m, the D 90 is 2.187 mu m, the uniformity and stability of the system are ensured, and when the emulsion droplet particle size D 50 and the D 90 are co