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CN-121986836-A - Fruit preservative containing trichodermin or analogues thereof and application thereof

CN121986836ACN 121986836 ACN121986836 ACN 121986836ACN-121986836-A

Abstract

A fruit preservative containing trichodermin or analogues thereof and application thereof belong to the technical field of fruit preservation. In order to reduce the postharvest loss of fruits caused by pathogen infection and imperfect preservation technology and solve the technical problem of safety risk of the existing fruit preservation technology, the invention provides a fruit preservative containing trichoderma or analogues thereof, which is prepared by dissolving trichoderma or analogues thereof in absolute ethyl alcohol, and can prolong the storage time of the fruits after harvest and inhibit the growth of pathogenic fungus hyphae causing the postharvest diseases of the fruits by fumigation, so as to further prevent and treat various postharvest diseases of the fruits. In addition, trichoderma analog is dissolved in water to serve as a fruit preservative, and fruits are preserved in a spraying mode, so that the effects of prolonging the storage time of the picked fruits and preventing and controlling various fruit postharvest diseases can be achieved.

Inventors

  • XIANG WENSHENG
  • ZHAO JUNWEI
  • XU XI
  • WANG XIANGJING

Assignees

  • 东北农业大学

Dates

Publication Date
20260508
Application Date
20241108

Claims (10)

  1. 1. The application of the fruit preservative containing trichodermin in inhibiting the growth of pathogenic fungi hyphae causing postharvest diseases of fruits is characterized in that the fruit preservative is obtained by dissolving trichodermin in absolute ethanol, the trichodermin is 2, 4-di-tert-butylphenol, and the application mode of the fruit preservative in the application is fumigation.
  2. 2. The use according to claim 1, wherein the pathogenic fungus is alternaria alternata (ALTERNARIA ALTERNATA), alternaria tenuis (ALTERNARIA TENUISSIMA), alternaria solani (ALTERNARIA SOLANI), botrytis cinerea (Botrytis cinerea), colletotrichum gloriosa (Colletotrichum gloeosporioides), colletotrichum rubrum (Colletotrichum horii) or penicillium digitatum (Penicillium digitatum).
  3. 3. The application of the fruit preservative containing trichodermin in preventing and treating fungal diseases of the picked fruits is characterized in that the fruit preservative is obtained by dissolving trichodermin in absolute ethyl alcohol, the trichodermin is 2, 4-di-tert-butylphenol, and the application mode of the fruit preservative in application is fumigation, wherein the fumigation concentration is 2-8 mug/cm 3 .
  4. 4. The use according to claim 3, wherein the post harvest fungal disease of fruit is black spot, fruit rot or gray mold.
  5. 5. The application of the fruit preservative containing trichodermin in prolonging the storage time of the picked fruits is characterized in that the fruit preservative is obtained by dissolving trichodermin in absolute ethyl alcohol, the trichodermin is 2, 4-di-tert-butylphenol, and the application mode of the fruit preservative in application is fumigation, wherein the fumigation concentration is 1-2 mug/cm 3 .
  6. 6. The application of the fruit preservative containing the trichostatin analogue in inhibiting the growth of pathogenic fungi hyphae causing fruit postharvest diseases is characterized in that the fruit preservative is obtained by dissolving the trichostatin analogue in absolute ethyl alcohol or water, the trichostatin analogue is tert-butylhydroquinone, the application is that the trichostatin analogue is dissolved in absolute ethyl alcohol, the pathogenic fungi are treated in a fumigation mode, or the trichostatin analogue is dissolved in water, and then the culture medium is added, and the pathogenic fungi are inoculated.
  7. 7. The use according to claim 6, wherein the pathogenic fungus is alternaria alternata (ALTERNARIA ALTERNATA), alternaria tenuis (ALTERNARIA TENUISSIMA), alternaria solani (ALTERNARIA SOLANI), botrytis cinerea (Botrytis cinerea), colletotrichum gloriosa (Colletotrichum gloeosporioides), colletotrichum rubrum (Colletotrichum horii) or penicillium digitatum (Penicillium digitatum).
  8. 8. The application of the fruit preservative containing the trichoderma analog in preventing and treating postharvest fungal diseases of fruits is characterized in that the fruit preservative is obtained by dissolving the trichoderma analog in absolute ethyl alcohol or water, the trichoderma analog is tert-butylhydroquinone, the application is that the trichoderma analog is dissolved in absolute ethyl alcohol, the fruit preservative is applied in a fumigation mode, the fumigation concentration is 2-8 mug/cm 3 , or the trichoderma analog is dissolved in water, the fruit preservative is applied in a spraying mode, and the trichoderma analog concentration is 1-2 mug/mL.
  9. 9. The use according to claim 8, wherein the post harvest fungal disease of fruit is black spot, fruit rot or gray mold.
  10. 10. The application of the fruit preservative containing the trichoderma analog in prolonging the storage time of the picked fruits is characterized in that the fruit preservative is obtained by dissolving the trichoderma analog in absolute ethyl alcohol or water, the trichoderma analog is tert-butylhydroquinone, the fruit preservative is applied by dissolving the trichoderma analog in absolute ethyl alcohol in a fumigation mode, the fumigation concentration is 1-2 mug/cm 3 , or the trichoderma analog is dissolved in water, and the fruit preservative is applied by spraying, wherein the concentration of the trichoderma analog is 1-2 mug/mL.

Description

Fruit preservative containing trichodermin or analogues thereof and application thereof Technical Field The invention belongs to the technical field of fruit preservation, and particularly relates to a fruit preservative containing trichoderma or analogues thereof and application thereof. Background Fruits are extremely susceptible to decay by pathogenic bacteria during growth and storage, resulting in significant economic losses. Fruit rot is not a regional problem, but a problem commonly existing in countries around the world, and reports of food and agricultural organizations (Food andAgriculture Organization ofthe UnitedNations, FAO) in united nations indicate that the post-harvest loss of fruits and vegetables in developed areas such as europe, north america and oceangoin is 29%, and the post-harvest loss of fruits and vegetables in areas such as asia, africa and latin america is 38%. Therefore, prevention of postharvest pathogen infection of fruits and preservation of postharvest fruits are of great significance in reducing postharvest losses of fruits. In the past, chemical antistaling agents play an important role in inhibiting fruit and vegetable decay and delaying fruit and vegetable aging. However, chemical antistaling agents have potential toxicity to human bodies and can destroy the ecological balance of the environment, and biological antistaling agents are natural substances, have better compatibility with the environment and higher safety, so that the biological antistaling agents are increasingly valued by people. 2,4-Di-tert-butylphenol (2, 4-Di-tert-butylphenol), a phenolic substance, is a constituent of many plant and microbial metabolites and is widely found in nature. 2,4-di-tert-butylphenol is a yellow solid, soluble in methanol and ether, very slightly soluble in water, and is commonly used as an antioxidant in production or as an intermediate in the production of antioxidants, with a toxic half-lethal dose LD50 (oral-rat) >2000mg/kg. The patent application with publication number CN105028406A discloses that 2,4-di-tert-butylphenol has bactericidal effect and can prevent and treat common diseases of fruits, vegetables, wheat, rice and the like, but in the patent application, the 2,4-di-tert-butylphenol acts on the production stage of fruits, vegetables or grain crops, prevents and treats field diseases (including apple, pear and peach scab, anthracnose, brown spot, ring spot and the like, powdery mildew, downy mildew, anthracnose and the like of grapes, watermelons, cucumbers and melons, grain crop diseases of wheat powdery mildew, rice blast, sheath blight and the like, powdery mildew, damping off, downy mildew, anthracnose and the like of vegetables) in the growth stage, and the application mode is spraying, and the 2,4-di-tert-butylphenol is not used for preventing and treating the diseases after fruit harvest. The use of 2,4-di-tert-butylphenol for preserving fruits is disclosed in the patent application CN104026220a, where fruits are directly soaked with an aqueous solution containing 2,4-di-tert-butylphenol, which presents a safety risk, and the patent application also does not disclose the broad spectrum activity of 2,4-di-tert-butylphenol against a variety of common postharvest pathogens. At present, no report of the prior art is found on the prevention and treatment of postharvest diseases of fruits or the preservation of fruits by using 2,4-di-tert-butylphenol in a mode other than soaking. Tert-butylhydroquinone (tert-Butylhydroquinone) is a registered food additive, which belongs to the class of fat-soluble antioxidants. In the food additive use sanitary standard (GB 2760-1996) of China, it is specified that tertiary butyl hydroquinone can be used for edible oil, fried food, dried fish products, biscuits, instant noodles, instant rice, dried fruit cans and salted meat products, and the maximum use amount is 0.2g/kg. At present, only one example of tertiary butyl hydroquinone is smeared for the application of longan fresh-keeping. Disclosure of Invention In order to reduce the postharvest loss of fruits caused by pathogen infection and imperfect preservation technology and solve the technical problem of safety risk of the existing fruit preservation technology, the invention provides a fruit preservative containing trichoderma or analogues thereof, which is prepared by dissolving trichoderma or analogues thereof in absolute ethanol and preserving fruits in a fumigation mode. The fruit preservative can prolong the storage time of the picked fruits by fumigation, inhibit the growth of pathogenic fungi hyphae causing the postharvest diseases of the fruits, further prevent and treat various postharvest diseases of the fruits, and has the advantages of safety and environmental protection. In addition, trichoderma analog is dissolved in water to serve as a fruit preservative, and fruits are preserved in a spraying mode, so that the effects of prolonging the storage time of the pi