CN-121986865-A - DDS slurry for degrading vomitoxin and glycerol and preparation method thereof

CN121986865ACN 121986865 ACN121986865 ACN 121986865ACN-121986865-A

Abstract

The invention relates to the technical field of agricultural and sideline product deep processing and feed additives, in particular to DDS slurry for degrading vomitoxin and glycerol and a preparation method thereof. According to the invention, the DDS slurry is inoculated with the specifically screened composite microbial inoculum, liquid fermentation is carried out under proper conditions, and microorganisms preferentially utilize free glycerol in the DDS slurry as a carbon source for metabolism, so that the glycerol content is remarkably reduced in the process, and the palatability is fundamentally improved. According to the invention, while the composite microbial inoculum is inoculated, the functional strain with vomitoxin degradation function and the special enzyme preparation are inoculated into the DDS slurry, the functional strain is subjected to field planting in the system and continuously produces enzyme, and the added enzyme preparation can rapidly and directly hydrolyze and destroy key toxicological groups in toxin molecules, so that the toxin molecules are converted into non-toxic or low-toxic degradation products.

Inventors

DUN YAOHAO
WANG YIMIN
LIN ZUO
LIANG YUNXIANG
GUO CHUNMIAO

Assignees

海伦市柏尊生物工程有限公司
青岛元通盛隆实业有限公司

Dates

Publication Date: 20260508
Application Date: 20251230

Claims (10)

1. The preparation method of the low vomitoxin and low glycerol fermentation DDS slurry is characterized by comprising the following steps of: Mixing DDS slurry, alkali and water to obtain a fermentation substrate; Mixing the fermentation substrate with a composite microbial inoculum, vomit-degrading enzyme and bacillus licheniformis (Bacillus licheniformis) BZ001, and performing fermentation culture to obtain fermentation DDS slurry; The composite microbial inoculum comprises bacillus coagulans (Bacillus coagulans) and streptococcus thermophilus (Streptococcus thermophilus); the preservation number of the bacillus licheniformis BZ001 is CCTCC M20252970.
2. The method according to claim 1, wherein the ratio of the effective viable count of the bacillus coagulans and the streptococcus thermophilus in the composite microbial inoculum is 0-1:1-0; the strain number of the bacillus coagulans is CICC 24625; The streptococcus thermophilus strain is CICC 20382.
3. The method according to claim 1, wherein the inoculation amount of the composite microbial inoculum is 1X 10 7 -1×10 8 CFU/g fermentation substrate.
4. The method of claim 1, wherein the amount of the emetic enzyme is 0.1% -0.2% of the mass of the fermentation substrate; The inoculation amount of the bacillus licheniformis BZ001 is 1 multiplied by 10 7 -1×10 8 CFU/g fermentation substrate.
5. The method of claim 1, wherein the base is calcium hydroxide or sodium hydroxide; The mass ratio of the DDS pulp to the alkali to the water is 1:0.02-0.03:0.5-1.
6. The method according to claim 1, wherein the fermentation culture is carried out at a temperature of 35-40 ℃ and a pH of 5.0-7.0 for 24-72 hours.
7. A fermented DDS pulp prepared by the preparation method of any one of claims 1-6.
8. Use of the fermented DDS pulp of claim 7 for the preparation of a feed additive or feed.
9. A feed additive or feed comprising the fermented DDS slurry of claim 7.
10. Use of the fermented DDS pulp of claim 7 or the feed additive or feed of claim 9 in any of the following: (1) Improving the growth performance of livestock and poultry; (2) Improving the immunity of livestock and poultry; (3) Improving the intestinal health of livestock and poultry.

Description

DDS slurry for degrading vomitoxin and glycerol and preparation method thereof Technical Field The invention relates to the technical field of agricultural and sideline product deep processing and feed additives, in particular to DDS slurry for degrading vomitoxin and glycerol and a preparation method thereof. Background DDS (DISTILLERS DRIED Solubes) slurries are the main by-product of the fuel ethanol and alcohol industry, and are all called distilled dry distillers' grains slurries. The DDS pulp contains rich protein, fat, fiber, vitamin and mineral, and is a feed raw material with low cost and high nutritive value. However, DDS slurries face two major technical bottlenecks in practical applications: First, palatability problems. During the fermentation of yeast to produce ethanol, a large amount of glycerol by-product is produced, resulting in a higher concentration of free glycerol (typically up to 5% -15%) in the final DDS slurry. The high content of glycerol can bring unpleasant sweet greasy feeling and pungent smell, and seriously affect the feeding willingness of livestock and poultry. Meanwhile, high content of glycerol leads to high viscosity of the DDS slurry, conveying is difficult, and good glycerol water-retaining property leads to difficult drying of the DDS slurry. These problems greatly limit the adding proportion and application effect of DDS slurry in the feed. Second, mycotoxin contamination problem. Cereal materials (e.g., corn, wheat, etc.) used to produce ethanol are extremely susceptible to infection by fusarium and other molds during storage or growth, producing mycotoxins, represented by vomitoxin. Vomitoxin has stable chemical properties, is difficult to effectively degrade in the processes of ethanol fermentation and subsequent drying, and can be completely remained in DDS pulp. After livestock and poultry ingest feed polluted by vomitoxin, a series of negative physiological reactions such as feed intake reduction, vomit, diarrhea, immunosuppression, growth retardation and the like can be caused, so that serious economic loss is caused for animal husbandry production, and food safety is threatened. Currently, in order to solve the problem of high glycerol content in DDS slurry, the method of physical dilution or adding flavoring agent is generally adopted for treatment, but the method has limited effect and is difficult to fundamentally solve the problem, and the cost effectiveness is not high in the long term. Meanwhile, the detoxification treatment of vomit toxin mainly comprises a physical adsorption method, namely using an adsorbent such as montmorillonite, yeast cell wall and the like, a chemical degradation method, namely breaking toxin structure by using chemical reagents such as alkali, oxidant and the like, and a biological degradation method, namely specifically degrading toxin by using specific microorganisms or enzyme preparations. However, there are significant limitations to the above methods. Although the physical adsorption method is simple and convenient to operate, the adsorption selectivity is poor, a large amount of nutrients can be adsorbed while toxins are removed, toxin molecules cannot be thoroughly destroyed, and potential risks exist. The chemical laws may introduce new chemical residues that affect the overall safety of the feed. Although the biological method has the advantages of mild conditions and strong specificity, in the matrix with complex components such as DDS slurry, the degradation efficiency is easily interfered by environmental factors, the stability is challenged, and more importantly, the biological degradation method only aims at toxin and has no improvement effect on the problem of excessive glycerin in the raw material. Therefore, the development of a comprehensive technical scheme capable of synchronously solving the problems of poor glycerol palatability and vomitoxin pollution in DDS pulp is urgently needed in the field so as to improve the quality of the DDS pulp and widen the application of the DDS pulp in high-value feeds. Disclosure of Invention The invention aims to provide DDS slurry for degrading vomitoxin and glycerol and a preparation method thereof, so as to solve the problems in the prior art. The invention constructs a composite symbiotic fermentation system consisting of high-efficiency glycerol metabolizing bacteria and special toxin degrading bacteria/enzymes, and realizes the synchronous and parallel occurrence of glycerol consumption and vomitoxin degradation in a unified process flow, namely the invention provides an improved DDS slurry and a biological fermentation-enzymolysis combined treatment method thereof, which can effectively degrade vomitoxin, obviously reduce glycerol content and improve palatability. The preparation method provided by the invention simplifies the operation, improves the efficiency and strengthens the synergistic effect of biological processes. In order to achieve the above object, the