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CN-121986871-A - Functional feed additive containing tea pigment light response cationic nano-liposome

CN121986871ACN 121986871 ACN121986871 ACN 121986871ACN-121986871-A

Abstract

The invention relates to a functional feed additive containing tea pigment photoresponse cationic nanoliposome, belonging to the technical field of feed additives. The functional feed additive comprises 25-35wt% of auxiliary materials and 65-75wt% of tea pigment light response cationic nano-liposome, wherein the tea pigment light response cationic nano-liposome is prepared from chitosan serving as a shell layer and a liposome serving as a core layer, and the liposome is prepared from soybean lecithin and cholesterol serving as capsule materials and a photosensitizer and tea pigment serving as core materials. The functional additive of the invention is added with auxiliary materials and tea pigment photoresponse cationic nanoliposome, and can effectively replace antibiotics by matching with optimized process parameters. The functional feed additive is especially suitable for livestock breeding such as chickens and ducks, and can better realize the accurate release of tea pigment by being assisted with blue light irradiation, thereby playing multiple effects of resisting bacteria, regulating immunity and improving meat quality.

Inventors

  • HU XIANCHUN
  • LIU QIANG
  • YANG XIAOFEN
  • LIU QIAN
  • CHEN PING
  • HUANG YE
  • YE YONG

Assignees

  • 广东广牧动物保健品有限公司

Dates

Publication Date
20260508
Application Date
20260130

Claims (10)

  1. 1. The functional feed additive containing the tea pigment photo-responsive cationic nano-liposome is characterized by comprising 25-35wt% of auxiliary materials and 65-75wt% of tea pigment photo-responsive cationic nano-liposome, wherein the tea pigment photo-responsive cationic nano-liposome is prepared by taking chitosan as a shell layer and taking liposome as a core layer, and the liposome is prepared by taking soybean lecithin and cholesterol as capsule materials and taking photosensitizer and tea pigment as core materials.
  2. 2. The functional feed additive containing tea pigment light response cationic nanoliposome according to claim 1, wherein the preparation method of the tea pigment light response cationic nanoliposome comprises the following steps: A1, preparing a tea pigment extract by taking branches and leaves of the black tea trees as raw materials and adopting an enzymatic oxidation method and a macroporous resin purification technology, wherein the enzymatic oxidation reaction is carried out at the pH of 4.5-5.5 and the temperature of 40-50 ℃; A2, dissolving capsule material soybean lecithin, cholesterol, a photosensitizer and the tea pigment extract prepared in the step A1 together in an organic solvent, removing the organic solvent under reduced pressure to form a lipid film, and hydrating the lipid film by using a buffer solution with the pH of 6.5-7.5 to obtain a primary liposome suspension for encapsulating the tea pigment and the photosensitizer, wherein the mass ratio of the soybean lecithin to the cholesterol is (5:1) - (8:1); A3, preparing a cationic chitosan acetic acid solution with the mass volume concentration of 0.05-0.15%, adding the solution into the primary liposome suspension obtained in the step A2 under the stirring condition, and coating chitosan on the liposome shell by utilizing electrostatic interaction to form a cationic nano liposome; and A4, homogenizing and purifying the product obtained in the step A3 to obtain the tea pigment photoresponse cationic nanoliposome.
  3. 3. A functional feed additive comprising tea pigment photo-responsive cationic nanoliposomes according to claim 1 or 2, wherein the photosensitizer is pheophorbide.
  4. 4. The functional feed additive containing tea pigment photoresponse cationic nano-liposome according to claim 2, wherein in the step A1, the enzyme used in the enzymatic oxidation reaction is polyphenol oxidase and/or peroxidase, and the addition amount of the enzyme is 0.1% -0.5% of the mass of the raw material; the macroporous resin is HPD-100 type or AB-8 type macroporous resin, and ethanol water solution with the concentration of 30% -60% is used for gradient elution; In the step A2, the mass of the tea pigment extract accounts for 10-20% of the total lipid mass by taking the total mass of the soybean lecithin and the cholesterol as the total lipid mass, and the mass ratio of the photosensitizer to the tea pigment extract is 1 (8-12); The organic solvent is at least one of chloroform, ethanol, methanol and dichloromethane, and the buffer solution is phosphate buffer solution.
  5. 5. The functional feed additive containing tea pigment photoresponsive cationic nanoliposome according to claim 2, wherein in step A3, the solvent of the cationic chitosan acetic acid solution is an aqueous acetic acid solution with a concentration of 0.5% -1.5%; In the step A4, the homogenization treatment is probe ultrasonic treatment, the ultrasonic power is 200-400W, the treatment time is 3-10 minutes, and the purification is dialysis or ultracentrifugation.
  6. 6. The functional feed additive containing the tea pigment photoresponse cationic nano-liposome is characterized by comprising, by weight, 30-50 parts of rice bran, 20-30 parts of montmorillonite, 12-20 parts of trehalose, 8-16 parts of fructo-oligosaccharide, 3-5 parts of choline, 0.2-0.5 part of salt and 0.8-1.5 parts of vitamin-trace element premix.
  7. 7. The functional feed additive containing the tea pigment light response cationic nano-liposome according to claim 6, wherein the auxiliary materials further comprise at least one of the following components, by weight, 0.2-0.4 part of riboflavin, 0.6-1 part of plant essential oil, 5-10 parts of vegetable oil, 2-4 parts of beta-glucan, 0.1-0.2 part of organic selenium, 0.1-0.3 part of lysine, 0.05-0.2 part of methionine and 3-5 parts of nano silicon dioxide.
  8. 8. A method for preparing a functional feed additive comprising tea pigment photo-responsive cationic nanoliposomes as claimed in any one of claims 1 to 7, wherein the method comprises the steps of: s1, mixing the components of the auxiliary materials, granulating at 35-45 ℃, and cooling to room temperature; S2, mixing the tea pigment photoresponse cationic nano-liposome with microcrystalline cellulose accounting for 10-15% of the weight of the tea pigment photoresponse cationic nano-liposome to prepare a functional premix; S3, adding the functional premix into the auxiliary material particles in the step S1, and mixing for 5-15 minutes at the temperature of 35-45 ℃; S4, adding a surfactant aqueous solution accounting for 0.8-1.2% of the weight of the auxiliary materials, continuously mixing for 5-10 minutes, then drying at 40-50 ℃ until the water content is 10-12%, cooling in a dark place, and sealing and packaging.
  9. 9. The method according to claim 8, wherein in step S4, the surfactant is a food grade sucrose fatty acid ester or tween-80, and the concentration of the aqueous solution is 5-10%.
  10. 10. Use of a functional feed additive comprising tea pigment photo-responsive nanoliposomes according to any one of claims 1-7 in chicken farming, wherein the functional feed additive is added to chicken staple food and is irradiated for 5-30 minutes daily with blue light having a wavelength of 400-480 nm.

Description

Functional feed additive containing tea pigment light response cationic nano-liposome Technical Field The invention belongs to the technical field of feed additives, and particularly relates to a functional feed additive containing tea pigment photoresponse cationic nanoliposomes. Background In modern intensive breeding industry, antibiotics are widely applied to the poultry breeding process as feed additives, and particularly in the breeding of traditional livestock such as chickens and ducks, the use of antibiotics has become an important means for improving the breeding success rate and economic benefit. These antibiotics mainly include tetracyclines, sulfonamides, quinolones, beta-lactams, and the like, and have the main functions of preventing diseases, promoting growth, and improving feed conversion rate. However, the negative effects of the long-term use of antibiotics in large quantities are increasingly pronounced. Firstly, antibiotics are difficult to completely metabolize in animals, often remain in livestock and poultry products such as meat, eggs and the like in the form of original or metabolic products, enter the human body through a food chain, possibly cause anaphylactic reaction, and seriously cause the imbalance of normal flora of the human body and reduce the immunity of the organism. Second, abuse of antibiotics accelerates the generation and spread of resistant strains. In addition, the waste water and waste materials containing antibiotics discharged by the breeding industry enter the ecological environment to pollute soil and water sources and destroy ecological balance, so that vicious circle is formed. In recent years, plant-derived additives have been attracting attention due to their natural, safe, residue-free properties. The tea pigment is a natural polyphenol oxidized polymer with multiple biological activities extracted from tea, mainly comprises theaflavin, thearubigin and theabrownin, has excellent effects of antioxidation, antibiosis, anti-inflammatory and the like, and has great potential in the fields of food, medicine and agriculture. However, tea pigments have many challenges in practical application, including firstly, poor water solubility and low stability of tea pigments, easiness in degradation and inactivation in feed processing and animal digestive tract environments, secondly, difficulty in realizing targeted delivery by a conventional adding mode and low bioavailability, thirdly, incapability of realizing accurate drug delivery according to different growth stages and pathological states, and difficulty in meeting the requirements of dynamic culture. At present, the report of preparing the tea pigment into the liposome is not seen, and the related research of combining the tea pigment with the visible light response release technology and the positive ion active targeting function is not seen. Therefore, the cationic nano liposome capable of releasing tea pigment in response to illumination stimulus is developed and applied to a functional feed additive, and has important scientific value and application prospect in solving the problem of antibiotic abuse, guaranteeing food safety and promoting green sustainable development of the breeding industry. Disclosure of Invention The invention aims to provide a functional feed additive containing tea pigment photoresponse cationic nanoliposome, which solves the problems of poor stability and inaccurate release of tea pigment in the prior art and has the characteristics of reducing the use of antibiotics and improving the survival rate. The aim of the invention can be achieved by the following technical scheme: A functional feed additive containing tea pigment photo-responsive cationic nano-liposome comprises 25-35wt% of auxiliary materials and 65-75wt% of tea pigment photo-responsive cationic nano-liposome, wherein the tea pigment photo-responsive cationic nano-liposome is prepared by taking chitosan as a shell layer and taking liposome as a core layer, and the liposome is prepared by taking soybean lecithin and cholesterol as capsule materials and taking photosensitizer and tea pigment as core materials. Wherein, the auxiliary materials are mainly used as physical carriers to protect the structural stability and fluidity of the tea pigment photoresponse cationic nanoliposome, ensure the more comprehensive function of the additive and enhance the overall efficacy. Further, the preparation method of the tea pigment light response cationic nanoliposome comprises the following steps: A1, preparing a tea pigment extract by taking branches and leaves of the black tea trees as raw materials and adopting an enzymatic oxidation method and a macroporous resin purification technology, wherein the enzymatic oxidation reaction is carried out at the pH of 4.5-5.5 and the temperature of 40-50 ℃; A2, dissolving capsule material soybean lecithin, cholesterol, a photosensitizer and the tea pigment extract prepared in the step A1 toge