CN-121986876-A - Nanometer feed additive for improving intestinal flora and preparation method thereof

Abstract

The invention belongs to the field of feed additives, and in particular relates to a nano feed additive for improving intestinal flora and a preparation method thereof, which comprises the following steps of (1) providing an electric arc furnace with pure copper as an anode and a graphite rod as a cathode, injecting absolute ethyl alcohol into the furnace body in a vacuum state in the electric arc furnace, controlling the arcing current to be 40-60A, purifying the electric arc furnace in an argon atmosphere for 12-48 hours to obtain Cu@C nano particles, and (2) carrying out oxidation reaction on the Cu@C nano particles in an oxygen-containing atmosphere at the reaction temperature of 200-350 ℃ for 1-5 hours, wherein the oxidized product is the nano feed additive. The nano feed additive disclosed by the invention can be used for preventing and treating germ infection, enhancing the immunity and the antioxidant stress function of organisms, improving intestinal flora and prolonging the service life of animals, is an effective and safe functional feed additive, is simple in preparation method and low in production cost, can realize large-scale preparation, and has an important application prospect.

Inventors

• LI JUNBIAO
• WANG HAN
• HU LIANG
• LI YONG
• LEI XIAOFEI
• LI HUAZHENG
• ZHAO XIRUI

Assignees

• 金隆铜业有限公司
• 铜陵有色金属集团股份有限公司

Dates

Publication Date

20260508

Application Date

20260320

Claims (6)

1. 1. A nano feed additive for improving intestinal flora is characterized in that nano particles with a core-shell structure, which are prepared by adopting a plasma arc method with pure copper as an anode and graphite rods as cathodes, and with nano copper as a core and graphite as a shell, are adopted, and the nano feed additive is prepared.
2. 2. A method for preparing the nano feed additive for improving intestinal flora according to claim 1, comprising the following steps: (1) Providing an electric arc furnace with pure copper as an anode and a graphite rod as a cathode, injecting absolute ethyl alcohol into the furnace body under the state of vacuum in the electric arc furnace, controlling the arcing current to be 40-60A, and purifying in argon atmosphere for 12-48 hours to obtain Cu@C nano particles; (2) And (3) carrying out oxidation reaction on the Cu@C nano particles in an oxygen-containing atmosphere, wherein the reaction temperature is 200-350 ℃ and the oxidation reaction time is 1-5 h, and the oxidized product is the nano feed additive.
3. 3. The method for preparing a nano feed additive for improving intestinal flora according to claim 2, wherein in the step (1), the vacuum degree of an electric arc furnace is 10 -4 Pa.
4. 4. The method for preparing a nano feed additive for improving intestinal flora according to claim 2, wherein in the step (2), the oxidation reaction of Cu@C nano particles is performed in a tube furnace.
5. 5. The method for preparing a nano feed additive for improving intestinal flora according to claim 2, wherein in the step (2), the oxygen-containing atmosphere is a mixed atmosphere of oxygen and argon, and the volume content of oxygen is 10-20%.
6. 6. The method for preparing a nano feed additive for improving intestinal flora according to claim 2, wherein the core structure of the nano feed additive in the step (2) is a compound of Cu, cu 2 O and CuO, and the mass ratio of Cu 2 O is more than 30%.

Description

Nanometer feed additive for improving intestinal flora and preparation method thereof Technical Field The invention belongs to the field of feed additives, and particularly relates to a nano feed additive for improving intestinal flora and a preparation method thereof. Background The most important output of copper smelting enterprises is commercial copper, and with the continuous progress and innovation of technology, new products are continuously developed, besides the recovery and extraction of some rare precious metals and valuable components in the copper smelting process, research and application exploration of copper in different fields are achieved, for example, nano feed additives containing copper elements are prepared. With the development of the large-scale, centralized and mechanized management conditions of the modern breeding industry, the high-density animal breeding is easy to cause malnutrition of livestock and poultry, outbreak of epidemic diseases of livestock and poultry and large-scale transmission. In order to reduce loss, the current breeding industry mainly uses antibiotics, high copper preparations and growth promoters as feed additives to prevent pathogen infection and supplement essential nutrient elements for animal growth. However, excessive use of antibiotics can cause pathogenic bacteria to generate drug resistance, reduce the immune function of livestock and poultry bodies, cause the livestock and poultry diseases to be more difficult to treat, and meanwhile, the irregular use of antibiotics, high copper and other preparations can cause animal body poisoning and a large amount of residues in the livestock and poultry bodies, thereby affecting the health of people and animals. Especially in the aspect of intestinal flora, microorganisms with strong sensitivity to related preparations can be influenced, and microorganisms with poor sensitivity, especially harmful flora, can be greatly propagated, so that the intestinal flora is unbalanced, thereby easily providing a trigger for invasion of in-vitro microbial pathogenic bacteria, carrying pathogenic bacteria in the food processing process, and causing cross infection. Therefore, the development and application significance of the green, safe and functional feed additive is great At present, copper sulfate is used as an economic and effective bactericide and a copper source for promoting growth, is a common feed additive in 'nonresistance' cultivation, has the core value of providing necessary copper element, and plays roles in promoting growth and resisting bacteria under pharmacological dosage. However, copper sulfate is a non-negligible hard hazard due to its low absorption, high emission and environmental pollution, especially in its high dose mode of use, and presents a significant challenge. In contrast, nano copper has significant advantages as a feed additive. On the one hand, the nano copper with the size of 1-100 nm generally has larger specific surface area and is easier to be absorbed through mechanisms such as endocytosis, pinocytosis and the like of intestinal wall cells, so that the nano copper has extremely high bioavailability. Research shows that the bioavailability of nano copper can reach 2 times of copper sulfate or even higher. On the other hand, the nano copper has stronger antibacterial activity and low preparation cost, can realize low-dosage use and large-scale production, and has wide application prospect in the field of feeds. However, the high surface energy of bare nano-copper, which leads to its easy oxidation and the risk of off-target, remains a major reason that restricts the widespread use of nano-copper. Disclosure of Invention The invention aims to provide a nano feed additive for improving intestinal flora, which has a core-shell structure (Cu-Cu 2 O-CuO@C) with a three-phase copper composite structure as a core and mesoporous graphite as a shell. In order to achieve the aim, the nano feed additive for improving intestinal flora adopts the technical scheme that nano particles with a core-shell structure, which are prepared by a plasma arc method with pure copper as an anode and graphite rods as cathodes, and with nano copper as a core and graphite as a shell, are adopted, and the nano feed additive is obtained. Another object of the present invention is to provide a method for preparing the nano feed additive for improving intestinal flora, comprising the following steps: (1) Providing an electric arc furnace with pure copper as an anode and a graphite rod as a cathode, injecting absolute ethyl alcohol into the furnace body under the state of vacuum in the electric arc furnace, controlling the arcing current to be 40-60A, and purifying in argon atmosphere for 12-48 hours to obtain Cu@C nano particles; (2) And (3) carrying out oxidation reaction on the Cu@C nano particles in an oxygen-containing atmosphere, wherein the reaction temperature is 200-350 ℃ and the oxidation reaction time is 1-5 h