CN-121986882-A - Semi-dry solid state fermentation rhizoma polygonati leaf-tikang chicken feed based on composite microbial inoculant

CN121986882ACN 121986882 ACN121986882 ACN 121986882ACN-121986882-A

Abstract

The invention discloses a semi-dry solid state fermentation rhizoma polygonati leaf tikang chicken feed based on a composite microbial inoculum, which is prepared by taking rhizoma polygonati leaves as a main raw material and carrying out semi-dry solid state fermentation under the condition that the water content is 18% -30%. The compound bacterial agent comprises fungus A and fungus B, wherein the fungus A is at least one selected from bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens, trichoderma reesei, aspergillus niger and aspergillus oryzae, and the fungus B is at least one selected from lactobacillus plantarum, lactobacillus rhamnosus, lactobacillus caseii, lactobacillus acidophilus and pediococcus pentosaceus. The invention realizes the concentration of cellulose content and polyphenol activity by using the composite microbial inoculum, and the fermentation product can be directly used as chicken feed or feed additive after being dried at low temperature, thereby being used for replacing the growth promotion/anti-inflammatory requirements of antibiotics.

Inventors

JIN YAO
WANG QIANYU
WEN SONGLIN
WU YI

Assignees

四川大学

Dates

Publication Date: 20260508
Application Date: 20260210

Claims (7)

1. A semi-dry solid state fermentation rhizoma polygonati leaf-substituted chicken resistant feed based on a composite microbial inoculum is characterized by being prepared by semi-dry solid state fermentation of rhizoma polygonati leaves serving as a main raw material under the condition that the water content is 18% -30%, wherein the composite microbial inoculum used for fermentation comprises fungus A and fungus B, the fungus A is at least one selected from bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens, trichoderma reesei, aspergillus niger and aspergillus oryzae, and the fungus B is at least one selected from lactobacillus plantarum, lactobacillus rhamnosus, lactobacillus caseii, lactobacillus acidophilus and pediococcus pentosaceus.
2. The semi-dry solid state fermentation rhizoma polygonati leaf tianti-chicken feed based on the composite microbial inoculum as claimed in claim 1, wherein the feed is prepared by semi-dry solid state fermentation of a mixture of rhizoma polygonati leaves, auxiliary materials and nutritional additives under the condition of water content of 18% -30%.
3. The semi-dry solid state fermentation rhizoma polygonati leaf tie resistant chicken feed based on the composite microbial agent of claim 2, wherein the auxiliary material is at least one selected from bran, corn flour, bean pulp, rice hull powder and straw powder.
4. The semi-dry solid state fermented sealwort leaf tie-resistant chicken feed based on a composite microbial inoculant of claim 2, wherein the nutritional additive is selected from at least one of molasses, glucose, calcium carbonate, and table salt.
5. The semi-dry solid state fermentation rhizoma polygonati leaf anti-chicken feed based on the composite microbial inoculum as claimed in claim 2, wherein the amount of the auxiliary materials is 0-30 wt% by mass, the amount of the molasses is 0-3 wt% by mass, the amount of the glucose is 0-2 wt% by mass, the amount of the calcium carbonate is 0-1 wt% by mass, the amount of the salt is 0-0.5 wt% by mass, and the total amount of the rest is 100% by mass, based on three total mass of rhizoma polygonati leaves, auxiliary materials and nutritional additives.
6. The semi-dry solid state fermented polygonatum sibiricum leaf tie resistant chicken feed based on the composite microbial agent of claim 1, wherein the composite microbial agent further comprises fungus C, and fungus C comprises saccharomyces cerevisiae and candida utilis.
7. The semi-dry solid state fermentation sealwort leaf tie-resistant chicken feed based on the composite microbial agent of claim 2, which is characterized by the following preparation method: S1, uniformly mixing crushed rhizoma polygonati leaves with auxiliary materials and nutritional additives, and controlling the water content of the mixture to be not more than 18% -30%; S2, adding the composite microbial inoculum into the mixture to enable the initial viable count to reach 10 6 -10 8 CFU/g, so as to obtain an inoculum; S3, placing the inoculum into a solid state fermentation device for fermentation, wherein the fermentation mode is one of the following three methods: (1) A closed anaerobic or micro-aerobic mode, wherein the temperature is 25-37 ℃, the temperature is 48-120 h, and the pH is less than or equal to 4.8; (2) Aeration and aerobic mode, wherein the temperature is 28-35 ℃, the temperature is 36-96 h, and the pile is turned over for 1-2 times during the aeration and aerobic mode; (3) Firstly, ventilating and aerobically at 28-35 ℃,24-48 h, and then sealing and anaerobically preserving 24-72 h; s4, drying the mixture at 35-60 ℃ by hot air or vacuum until the water content is less than or equal to 12% after fermentation, and then crushing the mixture to 20-60 meshes to obtain semi-dry fermented rhizoma polygonati She Fenliao which is directly used as a raw material or a feed additive of chicken feed.

Description

Semi-dry solid state fermentation rhizoma polygonati leaf-tikang chicken feed based on composite microbial inoculant Technical Field The invention relates to the technical field of animal nutrition and feed processing, in particular to a semi-dry solid state fermentation rhizoma polygonati leaf-replacement-resistant chicken feed based on a composite microbial inoculant. Background Antibiotics are often adopted in poultry breeding feeds for promoting growth or preventing medicines, but the problems of drug resistance, drug residue, unbalanced intestinal flora and the like are easy to cause after long-term use, and a 'replacement' technical route is needed in industry. The conventional engineering scheme of the feed replacement resistance mainly comprises two chemical means, namely an acidulant/organic acid and salts thereof (such as formic acid, propionic acid, butyrate and the like), and the like, and antibiotic dependence is reduced by regulating and controlling the pH of the gastrointestinal tract and bacteriostasis, but the scheme has the advantages of partial physicochemical bacteriostasis of action mechanism, relatively single functional emphasis, great influence on actual effects caused by daily ration buffering capacity, formula structure and feeding working condition, and fluctuation of stability. Secondly, the plant source is used as an antibiotic alternative, and the plant source additive is rich in active substances such as polyphenol, flavone, polysaccharide and the like, has the functions of antioxidation, immunoregulation and anti-inflammatory, and is widely studied. Mainly focuses on standardized extraction, compound synergy and stabilization application of plant extracts/essential oils/polyphenols and the like. For example, the existing feed products which take rhizoma polygonati (especially rhizoma polygonati leaves) as a core raw material and take 'anti-inflammatory-regulation intestinal tract-tikang' as a target positioning. The rhizoma polygonati is used as a plant for both medicine and food, a large amount of byproducts are produced by leaves of the rhizoma polygonati except for roots and stems in cultivation and processing, and the leaves are high in cellulose/hemicellulose content, poor in palatability, low in utilization rate, low in digestibility, insufficient in nutrient release and the like in direct feeding. The prior treatment mode related to the feeding of the Polygonatum sibiricum leaves has the defects that (1) the Polygonatum sibiricum leaves are directly crushed and dried to be used as coarse fodder, the fiber structure is complete, the cell wall is difficult to destroy, the active polyphenol release is insufficient, the chicken has limited tolerance to high fiber raw materials, and the addition amount is limited. (2) High moisture content wet fermentation (water content >40% or even 60%) can improve palatability and partial fiber degradation, but is easy to generate local putrefaction, mixed bacteria pollution and inconvenient storage and transportation, and stable powder can be prepared by drying with high energy consumption after fermentation. (3) Single strain fermentation, which is difficult to realize fiber reduction, acid production preservation and active substance enrichment at the same time, leads to large fluctuation of stability and functionality. Disclosure of Invention The semi-dry solid state fermentation polygonatum sibiricum leaf-replacement-resistant chicken feed based on the composite microbial inoculum provided by the invention aims to solve the technical problems of high fiber content, poor palatability, insufficient release of active ingredients, difficult storage and transportation due to wet fermentation, high drying energy consumption and the like of polygonatum sibiricum leaf byproducts. The method takes Polygonatum sibiricum leaves as raw materials, carries out semi-dry solid fermentation under the condition of low water content (less than or equal to 30 percent), realizes the concentration of cellulose content and polyphenol activity through a composite microbial inoculum system, and ensures that fermentation products can be directly used as chicken feed or feed additives after being dried at low temperature, thereby being used for replacing the growth promotion/anti-inflammatory requirements of antibiotics. The semi-dry solid state fermentation rhizoma polygonati leaf tikang chicken feed based on the composite microbial inoculum provided by the invention can be prepared by taking rhizoma polygonati leaves as raw materials and carrying out semi-dry solid state fermentation under the condition that the water content is 18% -30%. Or mixing rhizoma Polygonati leaf (optionally mixture of stems and leaves of rhizoma Polygonati) with adjuvants and nutritional additives to obtain mixture with water content of 18-30%, and inoculating compound microbial inoculum for semi-dry solid fermentation. The composite microbial inoculum used for fermentation compri