CN-121986884-A - Feed for hermetia illucens and raising method of hermetia illucens rich in superoxide dismutase

CN121986884ACN 121986884 ACN121986884 ACN 121986884ACN-121986884-A

Abstract

The invention belongs to the technical field of cultivation, and particularly relates to a feed for hermetia illucens and a raising method of the hermetia illucens rich in superoxide dismutase. Preparing high-acidity fruit and vegetable dreg slurry, mixing the high-acidity fruit and vegetable dreg slurry with a dry carrier to obtain a mixed material, adding a pH regulator into the mixed material to improve the pH, inoculating compound bacteria into the system, fermenting until the end point is thick to obtain feed, arranging a feeding promoting layer prepared by grain processing byproducts, inoculating black soldier fly larvae on the feeding promoting layer, placing the feed on the feeding promoting layer after the black soldier fly larvae stably eat to form a feed layer, and adding the high-acidity fruit and vegetable juice synergistic feed on the feed layer as a strengthening layer after the black soldier fly enters a quick weight increasing period before the pre-pupa. The method improves the palatability of the fruit and vegetable feed with high acidity value and realizes the efficient cultivation of functional hermetia illucens rich in SOD.

Inventors

ZHANG ZIYUE
QIAN HONGMEI
ZHOU PIN
YUAN XIAOQIAN
WANG LINGLING
LI YIPING
GU CONGCONG
GE DONGDONG
ZHU LIKE
Feng Wanning

Assignees

常州工学院
无锡市陆谱环保科技有限公司

Dates

Publication Date: 20260508
Application Date: 20260402

Claims (10)

1. The feed for the hermetia illucens is characterized by comprising the following steps of: a. The method comprises the steps of crushing high-acidity fruit and vegetable residues to obtain high-acidity fruit and vegetable residue slurry, mixing the high-acidity fruit and vegetable residue slurry with a dry carrier to obtain a mixed material, adding a pH regulator into the mixed material, and regulating the pH to 6-7; b. Inoculating a composite bacterium consisting of lactobacillus, saccharomycete and bacillus subtilis with the total number of viable bacteria not less than 10 8 CFU/g in the system, fermenting until the pH is stabilized at 6.0-6.5, and obtaining the feed.
2. The black soldier fly feed according to claim 1, wherein the particle size of the components in the high-acidity fruit and vegetable dreg slurry is not more than 5mm, the water content of the high-acidity fruit and vegetable dreg slurry is 80-85%, the water content of the mixed material is 60-65%, the addition amount of the dry carrier is 20-35% of the mass of the high-acidity fruit and vegetable dreg slurry, and the fermentation is carried out at 28-32 ℃ for 24-36 h.
3. The black soldier fly feed according to claim 1, wherein the high-acidity fruit and vegetable slag comprises one or more of roxburgh rose slag, passion fruit slag, lemon slag and sea buckthorn slag, the dry carrier comprises bran and leaf powder, and the grain processing by-product comprises one or more of bran, corn flour, rice bran and brewer's grains.
4. The black soldier fly feed according to claim 3, wherein the leaf powder comprises one or more of broussonetia papyrifera leaf powder, mulberry leaf powder and pagodatree leaf powder, and the mass ratio of the bran to the leaf powder is 2-3:1.
5. The hermetia illucens feed of claim 1, wherein the pH adjuster is a dispersion of modified calcium carbonate-based biomass or a plant ash leach.
6. The black soldier fly feed according to claim 5 is characterized in that the calcium carbonate-based biomass powder comprises one or more of eggshell powder and shell powder, the mass concentration of the dispersion liquid of the modified calcium carbonate-based biomass powder is 10-15%, the mass concentration of the plant ash leaching liquid is 5-10%, the modification method of the calcium carbonate-based biomass comprises the steps of cleaning, drying and crushing the calcium carbonate-based biomass, sieving the calcium carbonate-based biomass with a 80-100-mesh sieve at 400-600 ℃ for 1-3 hours, dispersing the calcined product in an alkaline solution with pH of 9-10, stirring the mixture for 1-2 hours, etching the mixture by using alkaline liquor, and filtering, washing and drying the mixture to finish modification.
7. The hermetia illucens feed of claim 1, wherein the inoculation amount of the composite microbial inoculum is 0.5-1.5% of the mass of the mixed material, the ratio of the viable count of lactic acid bacteria to that of bacillus subtilis is 1-2:1, and the ratio of the viable count of saccharomycetes to that of bacillus subtilis is 1-2:1.
8. A raising method of hermetia illucens rich in superoxide dismutase is characterized by comprising the steps of setting a feeding promoting layer prepared by grain processing byproducts, inoculating hermetia illucens larvae onto the feeding promoting layer, placing the feed according to any one of claims 1-7 on the feeding promoting layer after the hermetia illucens larvae stably eat the feed to form a feed layer, and adding high-acidity fruit and vegetable juice synergistic feed on the feed layer as a strengthening layer after the hermetia illucens enters a quick weight increasing period before the pre-pupa.
9. The raising method of the hermetia illucens rich in superoxide dismutase, which is characterized by comprising the steps of placing feed on a feeding promoting layer to form a feeding material layer with the thickness of 3-4 cm, covering new feed for 2-3 cm after feeding is reduced, controlling the total accumulated thickness of the feeding material layer to be not more than 12cm, and enabling the thickness range of the reinforcing layer to be 2-3 cm.
10. The method for preparing the raising method of the hermetia illucens rich in superoxide dismutase, which is characterized by comprising the step of adding high-acidity fruit and vegetable raw juice with the mass concentration of 10-20% into the feed, wherein the addition amount of the high-acidity fruit and vegetable raw juice is 5-10% of the feed mass.

Description

Feed for hermetia illucens and raising method of hermetia illucens rich in superoxide dismutase Technical Field The invention belongs to the technical field of cultivation, and particularly relates to a feed for hermetia illucens and a raising method of the hermetia illucens rich in superoxide dismutase. Background Rosa roxburghii as a special fruit in southwest of China is rich in vitamin C, flavonoid compounds, superoxide dismutase (SOD) and other active ingredients, and fruit residues accounting for about 20-30% of the weight of fresh fruits can be produced in the processing process of fruit juice, preserved fruits and the like. At present, the fruit residues are mostly treated as waste, and the fruit residues are easy to be decomposed and acidified and produce percolate to pollute the environment due to high acidity and high water content, so that the fruit residues become an environmental protection problem for the development of the Rosa roxburghii industry. Meanwhile, as a humic insect, the larvae of the hermetia illucens can efficiently convert organic wastes such as kitchen waste, livestock and poultry manure, fruit and vegetable wastes and the like into high-protein insect body feed and high-quality insect manure organic fertilizer, and the method is an important way for realizing agricultural recycling economy. For example, patent CN105433066a discloses a method for producing insect protein feed by using hermetia illucens to treat livestock manure, wherein the hermetia illucens larva is directly transferred into a treatment tank filled with livestock manure for fecal treatment, and finally the insect protein feed is obtained. The prior art for treating fruit and vegetable waste by using hermetia illucens mainly comprises three types, namely a direct mixed feeding method, a stack retting pretreatment method, a dry matter compounding method, a drying and crushing method for fruit and vegetable waste, and a compound feeding method for the fruit and vegetable waste after natural fermentation and putrefaction of the fruit and vegetable waste, wherein the first type is to simply mix apple pomace, grape pomace, vegetable tail vegetables and other auxiliary materials such as bran, rice bran and the like, and then directly feed the fruit and vegetable waste. However, when the prior art is directly applied to the roxburgh rose dreg, the obvious defects are that the high acidity of the roxburgh rose dreg can seriously inhibit the digestive enzyme activity of black soldier fly larvae when the mixed feeding is directly carried out, the feeding refusal rate of the larvae is up to more than 80 percent, the death rate is over 50 percent, the composting pretreatment needs 5 to 7 days, the period is long, the mixed bacteria are easy to breed to generate malodor, the spoilage process is uncontrollable, the active ingredients in the roxburgh rose dreg are lost in a large amount, the problem of partial peracid caused by uneven fermentation is still not solved, the dry matter compounding rule has high energy consumption and increased cost due to the drying process, and most of the active ingredients in the roxburgh rose dreg can be destroyed when the dried at high temperature, so that the potential value of the roxburgh rose dreg as a functional feed is lost. In addition, the enrichment research on functional components of hermetia illucens bodies is less in the prior art, as patent CN110338271A discloses an insect protein source feed and a processing method thereof, mainly aiming at maintaining the content of antibacterial peptide through high-temperature sterilization treatment and low-temperature drying treatment, but not relating to how to actively enrich specific functional components through a feeding process. Disclosure of Invention In order to solve the problems of high feeding rate, slow growth and high death rate of black soldier fly larvae after feeding untreated roxburgh rose residues caused by high acidity (pH value is usually between 3.0 and 4.0), high fiber content and high tannin content in the prior art, the invention mainly provides a feed and a feeding method for functional black soldier fly with rich SOD, which can efficiently convert the roxburgh rose residues into black soldier flies suitable for eating and enrich active ingredients such as SOD, and the specific scheme is as follows: a black soldier fly feed comprising the steps of: a. The method comprises the steps of crushing high-acidity fruit and vegetable residues to obtain high-acidity fruit and vegetable residue slurry, mixing the high-acidity fruit and vegetable residue slurry with a dry carrier to obtain a mixed material, adding a pH regulator into the mixed material, and regulating the pH to 6-7; b. Inoculating a composite bacterium consisting of lactobacillus, saccharomycete and bacillus subtilis with the total number of viable bacteria not less than 10 8 CFU/g in the system, fermenting until the pH is stabilized at 6.0-6.5, and obtain