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CN-121986913-A - Stage targeting scallop adductor phosphorus-free water-retaining agent containing anti-freeze glycopeptide and application thereof

CN121986913ACN 121986913 ACN121986913 ACN 121986913ACN-121986913-A

Abstract

The invention discloses a stage targeting scallop adductor phosphorus-free water-retaining agent containing an anti-freeze glycopeptide and application thereof, and relates to the technical field of food additives. The phosphorus-free water-retaining agent for the stage-targeted scallop adductor containing the anti-freeze glycopeptide and the application thereof comprise an agent A and an agent B, and do not contain phosphate additives, wherein the agent A is a penetrating anti-freeze liquid, and comprises, by mass, 5.0% -9.0% of trehalose, 3.0% -5.0% of sorbitol, 0.8% -1.5% of sodium citrate, 0.4% -1.0% of compound amino acid, 0.01% -0.1% of high-activity anti-freeze glycopeptide, 0.01% -0.05% of vitamin C, 0.01% -0.05% of rosemary extract and the balance of water. The stage targeting scallop adductor phosphorus-free water-retaining agent containing the anti-freeze glycopeptide and application thereof fundamentally inhibit ice crystal damage and protein denaturation, and realize cell-level protection of the scallop adductor. After detection, the thawing loss rate of the treated scallop adductor is as low as about 2.12 percent, the cooking loss rate is as low as about 10.63 percent, the water retention performance is similar to that of the commercial phosphate water retention agent, and the water retention agent is obviously superior to the existing phosphate-free water retention agent.

Inventors

  • DANG XIAOQING
  • CHEN YANGE
  • CUI YUJIE

Assignees

  • 正大云(青岛)生物科技有限公司

Dates

Publication Date
20260508
Application Date
20260126

Claims (10)

  1. 1. The stage targeting scallop adductor phosphorus-free water-retaining agent containing the anti-freeze glycopeptide is characterized by comprising an agent A and an agent B, and does not contain phosphate additives; The agent A is a permeation antifreeze liquid, and comprises 5.0-9.0% of trehalose, 3.0-5.0% of sorbitol, 0.8-1.5% of sodium citrate, 0.4-1.0% of compound amino acid, 0.01-0.1% of high-activity antifreeze glycopeptide, 0.01-0.05% of vitamin C, 0.01-0.05% of rosemary extract and the balance of water according to the total mass percentage; The agent B is a texture construction liquid, and comprises 0.8-1.5% of kappa-carrageenan, 0.03-0.15% of transglutaminase and the balance of water according to the total mass percentage.
  2. 2. The phase-targeted scallop adductor phosphorus-free water-retaining agent containing the anti-freeze glycopeptide of claim 1, wherein the compound amino acid is a mixture of L-arginine and L-lysine according to a mass ratio of 1:1-3:1.
  3. 3. The phase-targeted scallop adductor phosphorus-free water-retaining agent containing the anti-freeze glycopeptide of claim 1, wherein the high-activity anti-freeze glycopeptide is derived from antarctic cod and has a molecular weight of 2.6-3.5kDa.
  4. 4. The phase-targeted scallop adductor phosphorus-free water-retaining agent containing the anti-freeze glycopeptide of claim 1, wherein the viscosity of the kappa-carrageenan is more than or equal to 20000mPa seed s.
  5. 5. The phase-targeted phosphorus-free water-retaining agent containing the anti-freeze glycopeptide of claim 1, wherein the activity of transglutaminase is more than or equal to 100U/g.
  6. 6. The phase targeting scallop adductor phosphorus-free water retaining agent containing the anti-freeze glycopeptide according to claim 1, wherein the water retaining agent exists in a powder form and is dissolved in cold water to prepare a soaking solution with the concentration of 3% -8% w/v in use.
  7. 7. The method for preparing the phase-targeted scallop adductor phosphate-free water-retaining agent containing the anti-freeze glycopeptide according to any one of claims 1 to 6, which is characterized by comprising the following steps: S1, preparing a solution A, namely weighing trehalose, sorbitol, sodium citrate, compound amino acid, high-activity antifreeze glycopeptide, vitamin C and rosemary extract according to the mass percent of the agent A in claim 1, dissolving the components in ice water, and stirring until the components are fully dissolved; S2, preparing a solution B, namely weighing kappa-carrageenan according to the mass percent of the agent B in claim 1, completely dissolving the kappa-carrageenan in ice water, and then adding transglutaminase in a corresponding proportion, and gently stirring the mixture uniformly to avoid foam generation; S3, placing the prepared solution A and solution B in an environment of 0-4 ℃ for standby.
  8. 8. A method of treating scallop using the water retaining agent of any one of claims 1-6, comprising the steps of: step one, mixing scallop adductor and an agent A at 0-4 ℃ according to the mass ratio of 100:10-20, performing vacuum pulsation rolling and draining after being treated by a pulse electric field; step two, treating the scallop adductor treated in the step 1 with a B agent at 0-4 ℃, and standing at a stepped low temperature after ultrasonic assistance; and thirdly, performing ultra-fast monomer freezing on the scallop adductor processed in the step S2, so that the center temperature of the scallop adductor passes through a temperature range from minus 1 ℃ to minus 5 ℃ within 10 minutes.
  9. 9. The method for treating scallop meat by using the water-retaining agent according to claim 8, wherein the parameters of the pulsed electric field treatment in the first step are that the field intensity is 1-4kV/cm, the pulse width is 20-50 mu s, the total pulse action time is 50-300 mu s, the treatment temperature is controlled to be 0-6 ℃, the condition of vacuum pulsation rolling in the first step is that the temperature is 0-4 ℃, the vacuum degree is-0.08 to-0.095 MPa, and an intermittent rolling mode is adopted, rolling is carried out for 5min, and standing is carried out for 10min to form a cycle, and the total cycle is 2 times; The parameters of ultrasonic treatment in the second step are that the frequency is 20-45kHz, the power density is 0.2-0.5W/cm 2 , the treatment temperature is 0-4 ℃ and the treatment time is 1-5min; The step-type low-temperature standing in the second step comprises standing at 2-6deg.C for 10-20min, then at-2~0 deg.C for 20-40min, and finally at-4 to-2deg.C for 15-30min; In the third step, ultra-fast monomer freezing adopts a liquid nitrogen spraying or ultra-low temperature strong wind freezing mode; The pulse electric field treatment and vacuum tumbling in the first step are continuously carried out in a low-temperature environment at 0-4 ℃; The scallop is fresh scallop with white or yellowish color, compact tissue, no odor and no mechanical damage, and is subjected to cold water washing, cold storage at 0-4 ℃ and standing and draining treatment before use.
  10. 10. Use of a stage targeted scallop adductor phosphorus-free water retaining agent containing an anti-freeze glycopeptide according to any one of claims 1 to 6, comprising the steps of: Raw material pretreatment, namely selecting fresh shelled scallop columns which are white or slightly yellow in color, compact in structure, free of peculiar smell and mechanical damage, placing the fresh shelled scallop columns on a clean operation table, gently flushing the fresh shelled scallop columns with cold water to remove impurities, blood stains and residual viscera tissues attached to the surfaces, spreading the fresh shelled scallop columns on a stainless steel draining net rack, standing and draining the scallop columns in a refrigerating environment at 0-4 ℃ to avoid water accumulation from affecting the permeation efficiency of water retention liquid; Mixing the pretreated scallop adductors with an agent A at 0-4 ℃ according to the mass ratio of 100:10-20 to ensure that each scallop adductor is covered by the solution and has no bubble wrapping; Sealing the container, and then sending the container into a pulse electric field treatment chamber, and treating the container at 0-6 ℃ with parameters of field intensity of 1-4kV/cm, pulse width of 20-50 mu s and total pulse action time of 50-300 mu s; transferring the treated sample into a vacuum rolling machine in an environment of 0-4 ℃ for 2 times by adopting an intermittent mode of rolling for 5min and standing for 10min under the vacuum degree of-0.08 to-0.095 MPa; taking out scallop, placing on a perforated tray with holes at 0-4deg.C, draining for 10min, transferring into an environment of-2deg.C to 0deg.C, balancing, and standing for 30min; Ultrasonic auxiliary crosslinking and step temperature control treatment, namely uniformly immersing or spraying the drained scallop adductor into the solution B at the temperature of 0-4 ℃ and immediately taking out; Placing the single layer in a tray, and placing in a water bath of an ultrasonic cleaning tank at 0-4deg.C for treatment with parameters of 20-45kHz, 0.2-0.5W/cm 2 of power density and 1-5min; The step-type low-temperature standing is carried out after the treatment, namely, standing is carried out for 10-20min at the temperature of 2-6 ℃, then standing is carried out for 20-40min at the temperature of-2~0 ℃, and finally standing is carried out for 15-30min at the temperature of-4 to-2 ℃ so as to realize the precise control of the protein crosslinking degree by transglutaminase; Ultra-fast vitrification freezing, namely placing a scallop adductor monolayer processed by S2 on a stainless steel mesh belt, and carrying out ultra-fast monomer freezing by adopting a liquid nitrogen spraying or ultra-low temperature strong wind freezing mode, wherein the center temperature of the product is required to pass through a maximum ice crystal generation belt of-1 ℃ to-5 ℃ within 10min; And after the freezing is finished, immediately carrying out vacuum packaging, and storing in a refrigeration house at-25 ℃.

Description

Stage targeting scallop adductor phosphorus-free water-retaining agent containing anti-freeze glycopeptide and application thereof Technical Field The invention relates to the technical field of food additives, in particular to a stage targeting scallop adductor phosphorus-free water-retaining agent containing anti-freeze glycopeptide and application thereof. Background Scallop adductor is a high-quality ocean food material with high protein and low fat, and is widely used for various dishes such as tattooing, cooking, barbecue and the like, and has large market demand. However, during the processes of freezing storage, thawing and subsequent cooking, scallop meat is easy to cause problems of ice crystal formation and growth, protein denaturation, water loss and the like, so that the texture is hard, the elasticity is reduced, the taste is pulverized, and meanwhile, the loss of juice and nutrient components is accompanied, so that the commodity value of the product and the eating experience of consumers are seriously affected. Therefore, adding a proper water retention agent in the freezing preservation process is a key for maintaining the scallop meat quality; The water maintaining agent belongs to the field of food quality improver and has the core functions of raising the stability of food, maintaining the internal water content, improving the shape, flavor, color, etc. The most widely used water retention agent in the food field at present is phosphate, and the action mechanism of the water retention agent comprises that the pH value of aquatic products is improved and stabilized in a neutral range, the ionic strength is improved, the dissociation of actin is promoted, metal ions are chelated, and the like, so that the water retention performance and the gel strength of the aquatic products are improved. However, the application of the phosphate substances has the obvious defects that the long-term intake breaks the balance of calcium and phosphorus in human bodies, causes a series of health problems such as osteoporosis and kidney stones, and does not accord with the development trend of modern health foods; In order to solve the potential safety hazard of phosphate, a phosphorus-free water retention scheme appears in the prior art, but the water retention effect is limited, the texture improvement is not obvious, and the phosphate system is difficult to completely replace due to the fact that single components such as glycine, sorbitol and the like are mostly relied on. Therefore, developing a phosphorus-free composite water-retaining agent which is efficient, safe, environment-friendly and has good industrial feasibility becomes a technical problem to be solved in the field. Disclosure of Invention The invention aims to at least solve one of the technical problems in the prior art, and provides a stage targeting scallop adductor phosphorus-free water-retaining agent containing anti-freeze glycopeptide and application thereof, which can solve the problems. The invention provides a stage targeting scallop adductor phosphorus-free water-retaining agent containing anti-freeze glycopeptide, which consists of an agent A and an agent B and does not contain phosphate additives; The agent A is a permeation antifreeze liquid, and comprises 5.0-9.0% of trehalose, 3.0-5.0% of sorbitol, 0.8-1.5% of sodium citrate, 0.4-1.0% of compound amino acid, 0.01-0.1% of high-activity antifreeze glycopeptide, 0.01-0.05% of vitamin C, 0.01-0.05% of rosemary extract and the balance of water according to the total mass percentage; The agent B is a texture construction liquid, and comprises 0.8-1.5% of kappa-carrageenan, 0.03-0.15% of transglutaminase and the balance of water according to the total mass percentage. Preferably, the compound amino acid is a mixture of L-arginine and L-lysine according to a mass ratio of 1:1-3:1. Preferably, the high activity antifreeze glycopeptide is derived from antarctic cod and has a molecular weight of 2.6-3.5kDa. Preferably, the viscosity of the kappa-carrageenan is more than or equal to 20000mPa s. Preferably, the activity of the transglutaminase is more than or equal to 100U/g. Preferably, the water-retaining agent is in the form of a powder, which is dissolved in cold water to prepare a soaking solution with the concentration of 3-8% w/v. A preparation method of a stage-targeted scallop adductor phosphorus-free water-retaining agent containing anti-freeze glycopeptide comprises the following steps: S1, preparing a solution A, namely weighing trehalose, sorbitol, sodium citrate, compound amino acid, high-activity antifreeze glycopeptide, vitamin C and rosemary extract according to the mass percent of the agent A in claim 1, dissolving the components in ice water, and stirring until the components are fully dissolved; S2, preparing a solution B, namely weighing kappa-carrageenan according to the mass percent of the agent B in claim 1, completely dissolving the kappa-carrageenan in ice water