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CN-121986924-A - Emulsion for improving memory activity by DHA and preparation method and application thereof

CN121986924ACN 121986924 ACN121986924 ACN 121986924ACN-121986924-A

Abstract

The invention relates to the technical field of biological substance preparation. The invention provides a catechin-phospholipid complex and an emulsion prepared from the catechin-phospholipid complex for improving memory activity by DHA. The invention also provides application of the catechin-phospholipid complex or the DHA-improving memory activity-improving emulsion in preparation of health-care products. The emulsion prepared by adopting the catechin-phospholipid complex as the emulsifier can effectively and synergistically entrap, stably protect and target deliver the beta-carotene and DHA, enhance the bioavailability and multicomponent synergistic effect of the beta-carotene and DHA, realize the effect of improving the memory function, and simultaneously show good physical and chemical stability and digestion tolerance.

Inventors

  • MI HONGBO
  • LI XUEPENG
  • CHEN JINGXIN
  • YuWen Shuang
  • LI JIANRONG

Assignees

  • 渤海大学

Dates

Publication Date
20260508
Application Date
20260205

Claims (10)

  1. 1. A catechin-phospholipid complex is characterized by comprising catechin and phospholipid in a molar ratio of (1-6); The phospholipid is soybean lecithin.
  2. 2. The method for preparing a catechin-phospholipid complex as defined in claim 1, comprising the steps of: (a) Dissolving phospholipid in petroleum ether to obtain phospholipid solution; (b) Dissolving catechin in a mixed solvent prepared by mixing ethyl acetate and absolute ethyl alcohol in a volume ratio of (2-6): 1 to obtain catechin solution; (c) Mixing the phospholipid solution with catechin solution, stirring, and rotary evaporating to obtain catechin-phospholipid complex.
  3. 3. The method according to claim 2, wherein the concentration of the phospholipid solution is 40-50mg/mL and the concentration of the catechin solution is 1-2.5mg/mL.
  4. 4. The method of claim 2, wherein the temperature of the stirring in step (C) is 23-26 ℃, and the time of the stirring is 1.5-2.5 hours; The temperature of the rotary evaporation in the step (C) is 40-50 ℃, and the rotating speed of the rotary evaporation is 30-50rpm.
  5. 5. An emulsion for improving memory activity comprising DHA prepared from the catechin-phospholipid complex of claim 1.
  6. 6. The method for preparing the DHA-enhancing memory-improving active emulsion according to claim 5, comprising the following steps: (1) Dissolving beta-carotene in algae oil, performing ultrasonic treatment, and stirring to obtain an oil phase; (2) Dissolving catechin-phospholipid complex in ultrapure water to obtain water phase; (3) Mixing the oil phase and the water phase according to the volume ratio of (4-8) to 4, shearing, and homogenizing.
  7. 7. The preparation method according to claim 6, wherein the concentration of beta-carotene in the oil phase is 0.5-1.5mg/mL, and the DHA content in the algae oil is 60-75%; the mass concentration of catechin-phospholipid complex in the water phase is 4.5-7%.
  8. 8. The method of claim 6, wherein the time of the ultrasound in step (1) is 20-40min; The temperature of stirring is 22-26 ℃, and the stirring time is 10-15h.
  9. 9. The method according to claim 6, wherein the shearing speed in the step (3) is 11000 to 13000rpm, and the shearing time is 1 to 3min; The homogenizing pressure in the step (3) is 50-65MPa, the homogenizing temperature is 3-5 ℃, and the homogenizing times are 2-4 times.
  10. 10. Use of a catechin-phospholipid complex according to claim 1 or a DHA-enhancing memory-improving active emulsion according to claim 5 in the preparation of a health product.

Description

Emulsion for improving memory activity by DHA and preparation method and application thereof Technical Field The invention relates to the technical field of biological substance preparation, in particular to an emulsion for improving memory activity by DHA, a preparation method and application thereof. Background Docosahexaenoic acid (Docosahexaenoic acid, DHA) is a main structural component of brain and retina phospholipid membrane, has important effects on infant brain nerve development, maintaining adult cognitive function and preventing senile cognitive decline, and has been widely confirmed in physiological activity of improving memory and improving learning ability. Beta-carotene can be converted to vitamin a in humans, is a safe dietary source of retinol, and is critical to vision, immunity and cell growth. Meanwhile, the beta-carotene has health benefits such as antioxidation, anti-inflammatory and the like, and can be used as a singlet oxygen quencher and a free radical scavenger. However, the problems of the easy oxidation of DHA and the low water solubility and bioavailability of beta-carotene have limited practical applications. The prior art adopts emulsion, microcapsule and other means to carry out embedding. Conventional emulsions, although improving dispersibility, have insufficient antioxidant protection and lack the function of actively promoting absorption. Therefore, developing a novel delivery system which has high physical and chemical stability, can obviously improve the bioavailability of DHA and pertinently enhance the memory activity of DHA becomes a technical problem to be solved in the field. Disclosure of Invention The invention aims to provide an emulsion for improving memory activity by DHA, which is prepared by adopting catechin-phospholipid complex as an emulsifier, and can effectively and synergistically entrap, stably protect and target-deliver beta-carotene and DHA, enhance bioavailability and multicomponent synergistic effect of the beta-carotene and DHA, and realize the effect of improving memory function. In order to achieve the above object, the present invention provides the following technical solutions: the invention provides a catechin-phospholipid complex, which comprises catechin and phospholipid in a molar ratio of (1-6), wherein the phospholipid is soybean lecithin. The invention also provides a preparation method of the catechin-phospholipid complex, which comprises the following steps: (a) Dissolving phospholipid in petroleum ether to obtain phospholipid solution; (b) Dissolving catechin in a mixed solvent prepared by mixing ethyl acetate and absolute ethyl alcohol in a volume ratio of (2-6): 1 to obtain catechin solution; (c) Mixing the phospholipid solution with catechin solution, stirring, and rotary evaporating to obtain catechin-phospholipid complex. Preferably, the concentration of the phospholipid solution is 40-50mg/mL, and the concentration of the catechin solution is 1-2.5mg/mL. Preferably, the temperature of the stirring in step (C) is 23-26 ℃, and the stirring time is 1.5-2.5h. Preferably, the temperature of the rotary evaporation in step (C) is 40-50 ℃, and the rotation speed of the rotary evaporation is 30-50rpm. The invention also provides an emulsion for improving the memory activity of DHA, which is prepared by using the catechin-phospholipid complex. The invention also provides a preparation method of the DHA-enhancing memory-improving active emulsion, which comprises the following steps: (1) Dissolving beta-carotene in algae oil, performing ultrasonic treatment, and stirring to obtain an oil phase; (2) Dissolving catechin-phospholipid complex in ultrapure water to obtain water phase; (3) Mixing the oil phase and the water phase according to the volume ratio of (4-8) to 4, shearing, and homogenizing. Preferably, the concentration of beta-carotene in the oil phase is 0.5-1.5mg/mL, and the DHA content in the algae oil is 60-75%. Preferably, the mass concentration of catechin-phospholipid complex in the aqueous phase is 4.5-7%. Preferably, the ultrasonic treatment in the step (1) is carried out for 20-40min, the stirring temperature is 22-26 ℃, and the stirring time is 10-15h. Preferably, the speed of the shearing in the step (3) is 11000-13000rpm, and the time of the shearing is 1-3min. Preferably, the homogenizing pressure in the step (3) is 50-65MPa, the homogenizing temperature is 3-5 ℃, and the homogenizing times are 2-4 times. The invention also provides application of the catechin-phospholipid complex or the DHA-improving memory-improving active emulsion in preparation of health-care products. By adopting the technical scheme, the invention has the following beneficial effects: According to the technical scheme, the catechin-phospholipid complex is used as an emulsifier to prepare the emulsion for delivering the beta-carotene and the DHA, so that the emulsion not only has good physical and chemical stability and digestion tolerance, but