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CN-121986926-A - Preparation method of low-esterification HG type passion fruit pectin

CN121986926ACN 121986926 ACN121986926 ACN 121986926ACN-121986926-A

Abstract

The invention relates to a preparation method of low-esterification HG type passion fruit pectin, which comprises the following steps of S1, performing steam explosion on passion fruit peel, wherein the steam explosion pressure is 0.2-0.5 MPa, the maintenance pressure time is 60-120S, passion fruit peel powder is obtained, S2, adding distilled water into the passion fruit peel powder obtained in the S1, adjusting pH to be acidic, adding cellulase and hemicellulase for enzymolysis, obtaining enzymolysis extract, S3, centrifuging the enzymolysis extract obtained in the S2, collecting supernatant, S4, adding ethanol into the supernatant obtained in the S3 for mixing, standing, centrifuging, collecting precipitate, and drying to obtain the low-esterification HG type passion fruit pectin. Compared with the prior art, the invention does not destroy the main structure of pectin by the synergistic effect of steam explosion pretreatment and the compound enzyme consisting of cellulase and hemicellulase, can completely reserve HG fragments and improve the extraction rate of pectin.

Inventors

  • AI LIANZHONG
  • ZHANG HUI
  • XIE FAN
  • WANG GUANGQIANG
  • XIA YONGJUN
  • XIONG ZHIQIANG
  • SONG XIN
  • Yang Diejin

Assignees

  • 上海理工大学

Dates

Publication Date
20260508
Application Date
20260202

Claims (10)

  1. 1. The preparation method of the low-esterification HG type passion fruit pectin is characterized by comprising the following steps of: S1, performing steam explosion on passion fruit peel, wherein the steam explosion pressure is 0.2-0.5 MPa, and the maintenance pressure time is 60-120S, so as to obtain passion fruit peel powder; S2, adding distilled water into the passion fruit peel powder obtained in the step S1, adjusting the pH to be acidic, and adding cellulase and hemicellulase for enzymolysis to obtain an enzymolysis extract; s3, centrifuging the enzymolysis extracting solution obtained in the step S2, and collecting supernatant; And S4, adding ethanol into the supernatant obtained in the step S3, mixing, standing, centrifuging, collecting precipitate, and drying to obtain the low-esterification HG-type passion fruit pectin.
  2. 2. The method for preparing low-esterified HG-type passion fruit pectin according to claim 1, wherein in the step S1, passion fruit peel after steam explosion treatment is ground and sieved to obtain passion fruit peel powder.
  3. 3. The method for preparing low esterified HG-type passion fruit pectin as recited in claim 2 wherein the mesh number of the screen is 60 mesh.
  4. 4. The method of claim 1, wherein in step S2, the ratio of passion fruit peel powder to distilled water is 1 g:10 mL~1 g:50 mL.
  5. 5. The method of preparing low esterified HG type passion fruit pectin as recited in claim 1 wherein in step S2 the pH is adjusted to 5.
  6. 6. The method for preparing low-esterified HG type passion fruit pectin according to claim 1, wherein in the step S2, the mass ratio of cellulase to hemicellulase is 2:3, and the total mass of the cellulase and the hemicellulase is 1% -3% of the dry weight of passion fruit peel powder.
  7. 7. The method for preparing low-esterification HG-type passion fruit pectin according to claim 1, wherein in the step S2, the enzymolysis condition is that stirring is carried out in a water bath at 40-60 ℃ for 60-150 min.
  8. 8. The method for preparing low-esterified HG-type passion fruit pectin according to claim 1, wherein in the step S2, the enzymatic hydrolysis is finished and then the enzymatic hydrolysis is further performed by heating and inactivating the enzymes.
  9. 9. The method of claim 1, wherein in step S4, the volume of ethanol is (2-4) times the volume of the supernatant.
  10. 10. The method of preparing low esterified HG type passion fruit pectin according to claim 1, wherein in step S4, the precipitate obtained is collected and washed with ethanol several times.

Description

Preparation method of low-esterification HG type passion fruit pectin Technical Field The invention belongs to the technical field of natural plant extracts, and relates to a preparation method of low-esterification HG-type passion fruit pectin. Background Passion fruit, also called passion fruit and passion fruit, is a kind of tropical fruit rich in nutrition, and is commonly used in industry for producing preserved fruit, fruit juice and the like due to its unique flavor. The passion fruit peel accounts for 50% -55% of the fresh fruit weight, and is rich in active substances such as carbohydrate, dietary fiber and the like. However, in the food processing industry, passion fruit peel is often discarded in large quantities as waste, which pollutes the environment and also causes resource waste. Pectin content in the main stream raw materials in the industry is about 5% -20% in the citrus peel and apple pomace, and the pectin content in the passion fruit peel is about 15% -25% of dry weight, and the homogalacturonic acid (Homogalacturonan, HG) is generally higher than the pectin in the citrus and apple, so the passion fruit peel is a potential resource for extracting pectin. The pectin extracted from the waste pericarps not only can create economic value, but also can reduce environmental pollution, and accords with the recycling economy concept. HG-type pectin has important research value in terms of biological activity, for example, it is reported that HG-type pectin can promote cytokine production, and that the active （GUO Y, CHENG Y, ZHENG B, et al. Structural characterization and immunomodulatory activity of a novel pectin polysaccharide extracted from Gardenia jasminoides fruit[J]. Carbohydrate Polymers, 2025, 369: 124337. ）.HG -type pectin which activates immune activity of macrophages can promote growth of probiotics, and the natural HG pectin which maintains intestinal flora balance （ZHAO Y, WANG P, WANG D, et al. Gut microbiota and metabolic profile affected by pectic domains during in vitro rat fecal fermentation: A comparative study between different glycans rich in pectic monosaccharides[J]. Carbohydrate Polymers, 2025, 356: 123365.）. can induce apoptosis and migration of tumor cells, and simultaneously reduce adhesion capability of tumor cells, so that the purpose （ZHANG R, LI Y, GUAN F, et al. A homogalacturonan-rich pectic polysaccharide isolated from Lonicera japonica Thunb. modulates galectin-4-mediated bioactivity and anti-hepatocellular carcinoma activity[J]. International Journal of Biological Macromolecules, 2025, 302: 140618.）. of resisting tumors is achieved. Pectin is an acidic polysaccharide present in plant cell walls and intercellular layers, and pectin is tightly attached as a "binder" to the cell wall rigid cross-linked structure, resulting in difficulty in complete release of pectin during extraction, and thus extraction of pectin from natural plant materials is a cumbersome process. Currently, the extraction method of pectin in industry is mainly hot acid extraction method. The principle of the thermal acid extraction method is that the plant cell wall is hydrolyzed under the acidic environment and high temperature condition to fully crack and swell, so that the insoluble protopectin is converted into water-soluble pectin and released into the extraction solvent. However, the conventional acid extraction method requires a long heating time at a high temperature, and generates a large amount of acid waste liquid, which is costly to treat and causes environmental pollution. According to the current research report, chinese patent application CN2023114872601 discloses a pectin rich in RG-I structure and a preparation method thereof, wherein citrus grandis fruits are used as raw materials, lactobacillus plantarum is used for fermentation and then an acid extraction method is combined for preparing the pectin with low esterification RG-I, and the method needs to utilize microorganism fermentation, and is complex in process and long in time consumption. Chinese patent application CN2021101888373 discloses a low-ester pectin and a preparation method thereof, wherein the low-ester pectin is prepared by adopting an ultrahigh-pressure assisted alkaline process for degreasing, but the environment pollution is serious under the alkaline process condition, and the ultrahigh-pressure treatment is complex. Disclosure of Invention The invention aims to solve the problems of low extraction rate, water pollution and the like in the prior art when low-esterification HG type passion fruit pectin is extracted, thereby providing a preparation method of the low-esterification HG type passion fruit pectin. The aim of the invention can be achieved by the following technical scheme: The technical scheme of the invention is to provide a preparation method of low-esterification HG-type passion fruit pectin, which comprises the following steps: S1, performing steam explosion on passion fruit peel, wherei