CN-121986931-A - Preparation method and application of lentinus edodes soluble dietary fiber-bonded phenol

Abstract

The invention discloses a preparation method and application of mushroom soluble dietary fiber-bonded phenol. The preparation method comprises the steps of sieving mushrooms, dissolving the mushrooms in water, adding high-temperature resistant alpha-amylase to carry out amylase reaction, adding protease to carry out protease reaction after the amylase reaction is finished, adding saccharifying enzyme to carry out saccharifying enzyme reaction after the protease reaction is finished, centrifuging to obtain supernatant after the saccharifying enzyme reaction is finished, precipitating the supernatant in ethanol solution, centrifuging to obtain precipitate, and sequentially washing and freeze-drying the precipitate to obtain the mushroom soluble dietary fiber-bonded phenol. Animal experiments prove that the shiitake mushroom soluble dietary fiber combined phenol has remarkable effect of relieving the colonitis, is derived from medicine and food homologous shiitake mushrooms, has simple and convenient process and easy industrialization, realizes high-value utilization of shiitake mushroom resources, and can be used for preparing functional foods, health-care foods or biological medicine products for auxiliary treatment for relieving the colonitis.

Inventors

• LU QUN
• ZHANG QIONGDAN
• YAN XIANG
• SHEN YI
• LIANG HONGSHAN

Assignees

• 华中农业大学

Dates

Publication Date

20260508

Application Date

20260203

Claims (10)

1. 1. A preparation method of mushroom soluble dietary fiber-bonded phenol is characterized by comprising the following steps: (1) Sieving Lentinus Edodes, and dissolving in water; (2) Adding high temperature resistant alpha-amylase to perform amylase reaction; (3) After the amylase reaction is finished, protease is added for protease reaction; (4) After the protease reaction is finished, adding saccharifying enzyme to carry out saccharifying enzyme reaction; (5) And after the saccharifying enzyme reaction is finished, centrifuging to obtain a supernatant, precipitating the supernatant in an ethanol solution, centrifuging to obtain a precipitate, and sequentially washing and freeze-drying the precipitate to obtain the lentinus edodes soluble dietary fiber-bonded phenol.
2. 2. The method according to claim 1, wherein in the step (1), the mesh number of the screen is 80-100 mesh; in the step (2), the enzyme activity of the high temperature resistant alpha-amylase is 20000-25000U/ml, the temperature of amylase reaction is 95-100 ℃, and the time of amylase reaction is 30-35 min; The mass volume ratio of the lentinus edodes to the high temperature resistant alpha-amylase is 1:0.05-0.08 g/mL.
3. 3. The method according to claim 2, wherein the mesh number of the sieve is 80 mesh; the enzyme activity of the high temperature resistant alpha-amylase is 20000U/ml, the temperature of amylase reaction is 95 ℃, and the time of amylase reaction is 35 min; The mass volume ratio of the lentinus edodes to the high temperature resistant alpha-amylase is 1:0.05 g/mL.
4. 4. The method of claim 1, wherein in the step (3), the protease activity is 100-150U/mg, the protease reaction temperature is 40-45 ℃, and the protease reaction time is 30-35 min; The mass ratio of the lentinus edodes to the protease is 1:0.01-0.015.
5. 5. The method of claim 4, wherein the protease has an enzyme activity of 100U/mg, a protease reaction temperature of 40 ℃ and a protease reaction time of 30 min; The mass ratio of the lentinus edodes to the protease is 1:0.01.
6. 6. The method of claim 1, wherein in the step (4), the enzyme activity of the saccharifying enzyme is 100000-110000U/mL, the temperature of the saccharifying enzyme reaction is 40-45 ℃, and the time of the saccharifying enzyme reaction is 30-35 min; the mass volume ratio of the lentinus edodes to the saccharifying enzyme is 1:0.3-0.35 g/mL; In the step (5), the concentration of the ethanol solution is 90-95%.
7. 7. The method of claim 6, wherein the enzyme activity of the saccharifying enzyme is 100000U/ml, the temperature of the saccharifying enzyme reaction is 40 ℃, and the time of the saccharifying enzyme reaction is 30 min; The mass volume ratio of the lentinus edodes to the saccharifying enzyme is 1:0.3 g/mL, and the concentration of the ethanol solution is 95%.
8. 8. A shiitake mushroom soluble dietary fiber bonded phenol prepared by the preparation method of any one of claims 1-7.
9. 9. Use of a lentinula edodes soluble dietary fiber-bound phenol as claimed in claim 8 in the preparation of a functional food, health food or medicament for alleviating colitis.
10. 10. A functional food for relieving colonitis, characterized in that the functional food comprises the shiitake mushroom soluble dietary fiber-bonded phenol as claimed in claim 8.

Description

Preparation method and application of lentinus edodes soluble dietary fiber-bonded phenol Technical Field The invention relates to the technical field of food functional components and biological medicines, in particular to a preparation method and application of mushroom soluble dietary fiber and phenol. Background Colitis is a chronic intestinal disease mainly characterized by inflammation and injury of colonic mucosa, the pathogenesis of the colitis is closely related to unbalance of intestinal flora, excessive oxidative stress, abnormal release of inflammatory factors, impaired intestinal barrier function, and the symptoms of diarrhea, abdominal pain, mucopurulent bloody stool and the like are clinically presented, the living quality of patients is seriously influenced, and the risk of colorectal cancer is possibly increased after long-term repeated attacks. At present, the clinical treatment mainly comprises anti-inflammatory drugs and immunosuppressants, but has the problems of obvious side effects, easy generation of drug resistance, incapability of radical treatment and the like, so that the development of safe and effective diet therapy intervention products becomes a research hotspot in the field of colonitis prevention and treatment. The active ingredients in the functional food have unique advantages in the aspect of intestinal health maintenance due to high safety and mild action. Dietary fiber and phenolic substances are two widely studied intestinal health promoting factors, wherein the dietary fiber can play a probiotic role by regulating the structure of intestinal flora, promoting the generation of Short Chain Fatty Acids (SCFAs) and enhancing the intestinal barrier function, and the phenolic substances can remove free radicals, inhibit the expression of proinflammatory cytokines and reduce the inflammatory injury of the intestinal tract by virtue of obvious antioxidant and anti-inflammatory activities. Dietary fiber-bound phenols refer to complexes formed by covalent bonding of phenolic substances with dietary fiber through ester bonds, ether bonds or glycosidic bonds, which have stronger gastrointestinal stability and colon targeting compared with free phenols. Researches show that the compound can be metabolized and decomposed by intestinal flora in colon to slowly release phenolic active ingredients, and meanwhile, the probiotic characteristics of dietary fiber are reserved, so that the synergistic effect of the dietary fiber and phenols is realized. The physiological functions of the active ingredients of Lentinus edodes as a medicinal and edible fungus have been widely confirmed. The soluble dietary fiber rich in the lentinus edodes has remarkable immunoregulation effect, can activate macrophages and NK cells, improves the immune response capability of organisms, can regulate the balance of intestinal flora, reduces lipid absorption, and provides basic guarantee for intestinal health. Studies have also demonstrated that when the lentinula edodes soluble dietary fiber is used in combination with probiotics, disease activity can be reduced in a mouse model of ulcerative colitis by modulating intestinal flora, increasing butyric acid synthesis, and reducing pro-inflammatory cytokine production. The existing research on dietary fiber-bonded phenols is mostly focused on grains such as tartary buckwheat, wheat bran, rosa roxburghii tratt, white kidney bean peel and the like or fruit and vegetable processing byproducts, and the preparation of soluble dietary fiber-bonded phenols by taking mushrooms as raw materials is not yet studied, and the effect of relieving colonitis is systematically explored. When the existing mushroom source components are used for colitis intervention, only single active components such as polysaccharide and the like are focused for extraction, and the synergistic anti-inflammatory potential of the Soluble Dietary Fiber (SDF) of the mushroom and natural phenols is not utilized, so that the anti-inflammatory effect is limited. When the lentinol is independently applied, the lentinol is easily degraded by gastrointestinal digestive juice, and the effective concentration of the colonic lesion part is insufficient and the bioavailability is low. Disclosure of Invention The invention aims to overcome the defects of the prior art and provides a preparation method and application of mushroom soluble dietary fiber-bonded phenol. According to the invention, the natural combination characteristic of SDF and natural phenols of the lentinus edodes is utilized by preparing the soluble dietary fiber-bonded phenol of the lentinus edodes, and the synergistic effect of the SDF and the natural phenol can be realized without introducing exogenous substances, wherein the SDF is taken as a natural carrier to protect phenols from being degraded by gastrointestinal tracts, colon targeted delivery is realized, the bioavailability is improved, and meanwhile, a synergistic system is formed