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CN-121986934-A - Nutritional composition for fatty liver and liver protection and preparation method thereof

CN121986934ACN 121986934 ACN121986934 ACN 121986934ACN-121986934-A

Abstract

The invention discloses a nutritional composition for fatty liver and liver protection and a preparation method thereof, and relates to the technical field of preparation of nutritional health products. The preparation method comprises the steps of mixing lipid-soluble components such as a elephantopus scaber ethanol extract and hydroxylated modified soybean lecithin, adding absolute ethyl alcohol to prepare a hydrophobic phase solution, adding deionized water to prepare a hydrophilic phase solution to water-soluble components such as oligomeric walnut peptide, dripping the hydrophobic phase solution into the hydrophilic phase solution, preparing a nano composite carrier through high-shearing stirring, intermittent ultrasonic emulsification, curing and spray drying, mixing the nano composite carrier with hippocampus peptide and ganoderma lucidum polysaccharide, constructing a gradient release structure through double-layer hydroxypropyl methylcellulose coating, and finally adding the rest components to mix to prepare the uniform mixture for tabletting. The invention improves the bioavailability by the multicomponent synergistic lipid-lowering, liver cell repairing, oxidation resistance and inflammation resistance and the nano-carrier and gradient release technology, has remarkable liver-protecting lipid-lowering effect and strong storage stability, and is suitable for long-term conditioning of fatty liver people.

Inventors

  • YANG BIN
  • CHAO ZHIMAO
  • LI YAN
  • LI JIA

Assignees

  • 尔井制药(长沙)有限公司

Dates

Publication Date
20260508
Application Date
20260120

Claims (10)

  1. 1. The nutritional composition for fatty liver and liver protection is characterized by comprising, by mass, 8-10 parts of a elephantopus scaber alcohol extract, 5-7 parts of a curcumol extract, 10-12 parts of modified soybean phospholipids, 6-8 parts of oligomeric walnut peptides, 3-4 parts of glutathione, 5-6 parts of chitosan, 2-3 parts of L-citrulline, 4-6 parts of hippocampal peptides, 3-5 parts of ganoderan, 7-9 parts of bupleurum alcohol extract, 4-6 parts of medlar extract, 5-7 parts of slow-release oyster peptides, 3-5 parts of sea cucumber peptides, 3-4 parts of vitamin C, 2-3 parts of lactoferrin, 0.1-0.2 part of biotin, 1-2 parts of taurine, 3-4 parts of hydroxypropyl methyl cellulose, 1-2 parts of stevioside and 1-2 parts of magnesium stearate.
  2. 2. A method of preparing a nutritional composition for fatty liver and liver protection according to claim 1, comprising the steps of: (1) Mixing the elephantopus scaber ethanol extract, curcuma longa ethanol extract and modified soybean lecithin, adding absolute ethyl alcohol, and stirring and dissolving under the protection of nitrogen to obtain a hydrophobic phase solution; (2) Mixing the oligomeric walnut peptide, glutathione, chitosan and L-citrulline, adding deionized water, heating and stirring under argon atmosphere until the oligomeric walnut peptide, the glutathione, the chitosan and the L-citrulline are completely dissolved, and obtaining a hydrophilic phase solution; (3) Dropwise adding the hydrophobic phase solution prepared in the step (1) into the hydrophilic phase solution prepared in the step (2) at a constant speed, maintaining high-shear stirring, performing intermittent ultrasonic emulsification after the dropwise adding is finished, curing at a constant temperature after emulsification, and performing spray drying to prepare a nano composite carrier; (4) Mixing Hippocampus peptide and ganoderan with the nano composite carrier prepared in the step (3), spraying low-viscosity hydroxypropyl methylcellulose solution to form a surface layer, spraying high-viscosity hydroxypropyl methylcellulose solution to form a core layer, and constructing a double-layer release structure; (5) Adding bupleurum alcohol extract, medlar extract, slow-release oyster peptide, sea cucumber peptide, vitamin C, lactoferrin, biotin, taurine, stevioside and magnesium stearate into the double-layer release structure prepared in the step (4), and uniformly mixing to obtain a uniform mixed material for tabletting.
  3. 3. The preparation method of claim 2, wherein in the step (1), the addition amount of the absolute ethyl alcohol is 8-10 times of the total mass of the mixture of the elephantopus scaber alcohol extract, the curcumol extract and the modified soybean phospholipid, and the stirring speed is 300-400r/min, and the modified soybean phospholipid is hydroxylated modified soybean phospholipid.
  4. 4. The preparation method according to claim 2, wherein in the step (2), the addition amount of the deionized water is 10-12 times of the total mass of the mixture of the oligowalnut peptide, the glutathione, the chitosan and the L-citrulline, the heating temperature is 35-38 ℃, and the stirring speed is 400-500r/min.
  5. 5. The method of claim 4, wherein in step (2), the method of producing the oligowalnut peptide comprises the steps of: 2a) Taking walnut protein, adding deionized water with the mass of 5-8 times of the walnut protein, and stirring and dispersing uniformly; 2b) Regulating the pH value of the system to 7.0-8.0, adding alkaline protease accounting for 1% -3% of the mass of the walnut protein, and hydrolyzing for 2-3 hours at a constant temperature of 50-55 ℃; 2c) After the hydrolysis is completed, heating the system to 90-95 ℃, and preserving the heat for 15-20min to inactivate protease; 2d) Cooling the system to room temperature, centrifuging to obtain supernatant, separating with ultrafiltration membrane with molecular weight cut-off of 1000Da, and collecting the permeate; 2e) Freeze-drying the permeate to obtain the oligomeric walnut peptide; the oligomeric walnut peptide is a mixture of small molecular peptide fragments with the molecular weight below 1000Da, the peptide fragments consist of 2-6 amino acid residues, and the hydrolysis degree is 20% -30%.
  6. 6. The method according to claim 2, wherein in the step (3), the constant-speed dropping speed is 1-1.5mL/min, and the high-shear stirring speed is 800-900r/min; The intermittent ultrasonic emulsification parameters are that emulsification is suspended for 2-4min for 20-40s, repeated for 3-5 times, ultrasonic power is 500-700w, and temperature is 40-50 ℃; the spray drying parameters are that the air inlet temperature is 110-120 ℃, the air outlet temperature is 55-60 ℃ and the atomization pressure is 0.3-0.4MPa.
  7. 7. The method according to claim 2, wherein in the step (4), the low-viscosity hydroxypropyl methylcellulose solution has a mass concentration of 4-6%, a coating thickness of 20-25 μm, and the high-viscosity hydroxypropyl methylcellulose solution has a mass concentration of 7-9%, and a coating thickness of 30-40 μm.
  8. 8. The method according to claim 7, wherein in the step (4), the coating is performed by a fluidized bed coating machine, the air inlet temperature of the surface coating is 42-45 ℃, the air outlet temperature is 32-35 ℃, the air inlet temperature of the inner core coating is 40-42 ℃, and the air outlet temperature is 30-32 ℃.
  9. 9. The method of claim 8, wherein in the step (4), the slow-release oyster peptide is prepared by the steps of: 4a) Pulverizing oyster meat, adding deionized water 7-10 times of the oyster meat, stirring and dispersing uniformly; 4b) Regulating the pH value of the system to 7.0-8.0, adding 3-5% of compound protease by mass of oyster meat, wherein the mass ratio of alkaline protease to neutral protease in the compound protease is 1:1, and hydrolyzing for 4-6 hours at the constant temperature of 50-55 ℃; 4c) After the hydrolysis is completed, heating the system to 90-95 ℃, and preserving the heat for 15-20min to inactivate protease; 4d) Cooling the system to room temperature, centrifuging to obtain supernatant, separating with ultrafiltration membrane with molecular weight cut-off of 800Da, collecting the permeate, and lyophilizing to obtain basic oyster peptide; 4e) Mixing basic oyster peptide and chitosan according to the mass ratio of 1:0.3-0.5, adding deionized water to prepare suspension, and spray drying to prepare microcapsule-embedded slow-release oyster peptide; The molecular weight of the basic peptide segment of the slow-release oyster peptide is 500-800Da, and the peptide segment with the molecular weight of 500-800Da accounts for more than or equal to 70 percent.
  10. 10. The preparation method according to any one of claims 1 to 9, wherein the sea horse peptide and sea cucumber peptide have a peptide segment with a molecular weight of 500-800Da accounting for not less than 70%.

Description

Nutritional composition for fatty liver and liver protection and preparation method thereof Technical Field The invention relates to the technical field of preparation of nutritional health-care products, in particular to a nutritional composition for fatty liver and liver protection and a preparation method thereof. Background With the change of life style and the upgrade of diet structure of people, the incidence of fatty liver caused by high-fat, high-sugar and high-calorie diet is rising year by year, and the fatty liver has become a high-rise liver metabolic disease in the global scope. The core pathological characteristics of fatty liver are excessive accumulation of fat in liver, and liver cell inflammation, oxidative stress and liver function injury are accompanied, if the fatty liver progresses for a long time, nonalcoholic steatohepatitis, liver fibrosis and even liver cirrhosis can be induced, and the human health is seriously threatened. Currently, products for conditioning and protecting fatty liver mainly comprise three types of chemical medicines, chinese herbal compounds and nutritional supplements. The traditional Chinese medicine compound has the advantages of high safety, complex ingredients, slow effect and high quality control difficulty, the existing nutritional supplements have the problems of single ingredients, poor lipid-water compatibility, low bioavailability of active ingredients and the like, namely, fat-soluble active ingredients (such as curcumin and elephantopus) are easy to agglomerate, water-soluble ingredients (such as peptides and vitamins) are easy to degrade, and an accurate release regulation mechanism is lacking, so that the active ingredients cannot reach liver targets efficiently, and the liver protecting and lipid lowering effects are limited. In addition, the peptide components added into partial products have disordered molecular weight distribution, low intestinal absorption efficiency and insufficient synergistic effect with other components, and the comprehensive conditioning effects of repairing liver cells, regulating lipid metabolism and resisting oxidative damage are difficult to realize. Therefore, there is a need to provide a nutritional composition for fatty liver and liver protection and a preparation method thereof, which solve the above technical problems. Disclosure of Invention The invention aims to provide a nutritional composition for fatty liver and liver protection and a preparation method thereof, which are used for solving the technical problems of agglomeration of lipid and water components, poor absorption of active components and limited liver protection and lipid reduction effects of the existing products. In order to achieve the above purpose, the present invention provides the following technical solutions: The invention provides a nutritional composition for fatty liver and liver protection, which comprises, by mass, 8-10 parts of a elephantopus scaber alcohol extract, 5-7 parts of a curcumol extract, 10-12 parts of modified soybean phospholipids, 6-8 parts of oligomeric walnut peptides, 3-4 parts of glutathione, 5-6 parts of chitosan, 2-3 parts of L-citrulline, 4-6 parts of hippocampus peptides, 3-5 parts of ganoderan, 7-9 parts of a bupleurum alcohol extract, 4-6 parts of a medlar extract, 5-7 parts of slow-release oyster peptides, 3-5 parts of sea cucumber peptides, 3-4 parts of vitamin C, 2-3 parts of lactoferrin, 0.1-0.2 part of biotin, 1-2 parts of taurine, 3-4 parts of hydroxypropyl methyl cellulose, 1-2 parts of stevioside and 1-2 parts of magnesium stearate. In a second aspect, the present invention provides a method of preparing a nutritional composition for fatty liver and liver protection, comprising the steps of: (1) Mixing the elephantopus scaber ethanol extract, curcuma longa ethanol extract and modified soybean lecithin, adding absolute ethyl alcohol, and stirring and dissolving under the protection of nitrogen to obtain a hydrophobic phase solution; (2) Mixing the oligomeric walnut peptide, glutathione, chitosan and L-citrulline, adding deionized water, heating and stirring under argon atmosphere until the oligomeric walnut peptide, the glutathione, the chitosan and the L-citrulline are completely dissolved, and obtaining a hydrophilic phase solution; (3) Dropwise adding the hydrophobic phase solution prepared in the step (1) into the hydrophilic phase solution prepared in the step (2) at a constant speed, maintaining high-shear stirring, performing intermittent ultrasonic emulsification after the dropwise adding is finished, curing at a constant temperature after emulsification, and performing spray drying to prepare a nano composite carrier; (4) Mixing Hippocampus peptide and ganoderan with the nano composite carrier prepared in the step (3), spraying low-viscosity hydroxypropyl methylcellulose solution to form a surface layer, spraying high-viscosity hydroxypropyl methylcellulose solution to form a core layer, and