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CN-121986936-A - Natural plant extract-nicotinamide composite preparation with targeted multi-mechanism synergistic anti-aging function and preparation method and application thereof

CN121986936ACN 121986936 ACN121986936 ACN 121986936ACN-121986936-A

Abstract

The invention relates to a targeted multi-mechanism synergistic anti-aging natural plant extract-nicotinamide composite preparation, and a preparation method and application thereof, belonging to the technical field of natural active ingredient application. The compound preparation comprises 8-15 parts of nicotinamide microcapsule powder, 10-25 parts of roxburgh rose extract, 0.001-0.003 part of yeast selenium, 15-30 parts of blueberry extract and 20-40 parts of plantain seed husk powder. The invention also provides a preparation method of the compound preparation and application of the compound preparation in preparation of functional foods or health care products. The compound preparation formula takes nicotinamide as a core repairing component, is compounded with the Rosa roxburghii extract, selenium, blueberry extract and Plantago ovata husk powder, realizes the multi-component synergistic effect by optimizing the combination proportion and the innovative preparation process, can remarkably reduce the mutation rate of cells, repair damaged DNA, strengthen the oxidation resistance of cells, regulate the metabolism of cells, and achieves the synergistic anti-aging effect from multiple angles and multiple mechanisms. And has the advantages of natural safety, high stability and high bioavailability.

Inventors

  • WANG YUNSHAN
  • GAO LIHE
  • LI WENJING

Assignees

  • 山东省大健康精准医疗产业技术研究院

Dates

Publication Date
20260508
Application Date
20260309

Claims (10)

  1. 1. The natural plant extract-nicotinamide composite preparation with the targeted multi-mechanism synergistic anti-aging effect is characterized by comprising the following raw materials in parts by weight: 8-15 parts of nicotinamide microcapsule powder, 10-25 parts of roxburgh rose extract, 0.001-0.003 part of yeast selenium, 15-30 parts of blueberry extract and 20-40 parts of Plantago ovata husk powder.
  2. 2. The targeted multi-mechanism synergistic anti-aging natural plant extract-nicotinamide composite preparation according to claim 1 is characterized by comprising, by weight, 10-12 parts of nicotinamide microcapsule powder, 15-20 parts of roxburgh rose extract, 0.002-0.003 part of yeast selenium, 20-25 parts of blueberry extract and 25-35 parts of Plantago ovata husk powder.
  3. 3. The targeted multi-mechanism synergistic anti-aging natural plant extract-nicotinamide composite preparation of claim 1, which is characterized by comprising the following raw materials, by weight, 10 parts of nicotinamide microcapsule powder, 18 parts of roxburgh rose extract, 0.003 part of yeast selenium, 22 parts of blueberry extract and 30 parts of Plantago ovata husk powder.
  4. 4. The targeted multi-mechanism synergistic anti-aging natural plant extract-nicotinamide composite formulation of claim 1, wherein the nicotinamide microcapsule powder is prepared according to the following method: a. adding Plantain seed husk powder and soybean protein isolate into deionized water, stirring and mixing uniformly, and regulating the pH value to 6.0-7.0 to obtain a wall material solution with the mass concentration of 12-18%; b. Adding nicotinamide into deionized water, stirring and mixing uniformly, adding EDTA, and stirring until the nicotinamide is completely dissolved to obtain a core material solution with the mass concentration of 8-15%; c. adding the core material solution into the wall material solution, stirring for 25-35 min at the temperature of 25-35 ℃ and the speed of 600-1000 r/min to obtain a mixed solution, adding sucrose fatty acid ester into the mixed solution, continuously stirring for 10-20 min to obtain emulsion, and freeze-drying to obtain nicotinamide microcapsule powder.
  5. 5. The targeted multi-mechanism synergistic anti-aging natural plant extract-nicotinamide composite preparation of claim 1, wherein in step a, the mass ratio of psyllium husk powder to soy protein isolate is (2.5-3.5): 1; in the step b, the addition amount of EDTA is 0.03-0.07% of the total mass of the core material solution; In the step c, the mass ratio of the core material solution to the wall material solution is 1 (3.5-4.5), and the addition amount of the sucrose fatty acid ester is 0.15-0.25% of the total mass of the mixed solution; the specific process of freeze drying comprises the steps of pre-freezing the emulsion for 3-5 hours at the temperature of minus 35 ℃ to minus 45 ℃, transferring the emulsion into freeze drying equipment after the pre-freezing is finished, controlling the drying temperature to minus 55 ℃ to minus 45 ℃ and the vacuum degree to be 0.05-0.15 Pa, and continuously freeze drying for 20-28 hours.
  6. 6. The targeted multi-mechanism synergistic anti-aging natural plant extract-nicotinamide composite formulation of claim 1, wherein the rosa roxburghii tratt extract is prepared according to the following method: Removing impurities from fresh fructus Rosae Normalis, cleaning, removing cores, crushing to particle size of 3-10 mm, adding vitamin C, stirring and mixing to obtain pulp slurry, adding a compound enzyme preparation into pulp slurry, regulating pH to 4.0-5.0, performing enzymolysis at 30-40deg.C for 1.5-3 hr, inactivating enzyme, centrifuging at 3000-5000 r/min for 10-20 min, collecting supernatant, filtering, purifying, concentrating to obtain concentrated solution, adding a compound carrier into concentrated solution, stirring and mixing uniformly, and spray drying to obtain fructus Rosae Normalis extract; wherein the addition amount of the vitamin C is 0.05-0.2% of the mass of the pulp; the compound enzyme preparation is prepared by compounding pectase, cellulase and xylanase according to the mass ratio of 1 (1.5-2.5) (0.3-0.7), and the addition amount is 0.2-0.5% of the mass of pulp slurry; In the enzymolysis process, continuously introducing nitrogen or argon at a flow rate of 30-80 mL/min, wherein the enzyme deactivation is carried out by heating in a water bath at 75-85 ℃ for 8-15 min, and then cooling to room temperature; The specific procedures of filtering, purifying and concentrating comprise the steps of filtering supernatant by a microfiltration membrane with the thickness of 0.22-0.45 mu m, loading the filtered supernatant to a D101 or AB-8 macroporous resin column, controlling the loading flow rate to be 1-3 BV/h, eluting impurities by deionized water, eluting active ingredients by a gradient of 25-35% edible alcohol, and collecting an edible alcohol eluent, and concentrating the eluent under reduced pressure at 45-55 ℃ and-0.07-0.09 MPa until the solid content is 20-30%; the composite carrier is prepared by compounding maltodextrin and beta-cyclodextrin according to the mass ratio of (2-3) to 1, and the addition amount of the composite carrier is 20-40% of the mass of solid matters in the concentrated solution; the spray drying method comprises the specific procedures of adopting a centrifugal spray dryer, controlling the air inlet temperature to be 150-170 ℃, the air outlet temperature to be 65-75 ℃ and the feeding rate to be 10-20 mL/min, and drying until the water content of the roxburgh rose extract is less than or equal to 5%.
  7. 7. The targeted multi-mechanical synergistic anti-aging natural plant extract-nicotinamide composite formulation of claim 1, wherein the blueberry extract is prepared according to the following method: Freeze-drying fresh blueberry fruits, crushing to 60-100 meshes to obtain blueberry powder, adding edible alcohol into the blueberry powder according to the feed liquid ratio of 1g (15-25 mL), extracting for 20-40 min under the conditions that the ultrasonic power is 250-350W and the extraction temperature is 35-45 ℃, repeatedly extracting for 1-2 times, combining all the extracting solutions, centrifuging the combined extracting solutions for 15-25 min under the condition of 4000-6000 r/min, taking supernatant, purifying, eluting and drying in vacuum to obtain blueberry extract; The purification, elution and vacuum drying specific procedures comprise loading supernatant onto an AB-8 macroporous resin column, controlling the loading flow rate to be 1.0-2.0 BV/h, eluting impurities with 15-25% edible alcohol, eluting anthocyanin with 50-70% edible alcohol, collecting target eluent, and vacuum drying the eluent at 40-50 ℃ and-0.08-0.095 MPa to obtain the blueberry extract.
  8. 8. The method for preparing the targeted multi-mechanism synergistic anti-aging natural plant extract-nicotinamide composite preparation as claimed in claim 1, which is characterized by comprising the following steps: (1) Sieving the Rosa roxburghii extract, the blueberry extract and the Plantago ovata husk powder with a sieve of 80-120 meshes respectively, crushing the selenium yeast to 180-220 meshes, and sieving the nicotinamide microcapsule powder with a sieve of 80-120 meshes; (2) Firstly, putting the roxburgh rose extract, the blueberry extract and the Plantago ovata husk powder into a three-dimensional mixer according to the proportion, and mixing for 10-20 min at 15-25 r/min to obtain a premix; (3) Adding deionized water into the premix, and uniformly stirring and mixing to obtain a soft material, wherein the addition amount of the deionized water is 6-10% of the total mass of the premix; (4) Granulating the soft material after passing through a 18-24-mesh sieve, drying wet granules in a 45-55 ℃ blast drying oven for 2-3 hours, controlling the moisture content of the granules to be less than or equal to 5%, and finishing the granules after drying through a 16-20-mesh sieve to obtain primary granules; (5) And (3) adding nicotinamide microcapsule powder and yeast selenium into the primary particles according to the proportion, placing the mixture into a two-dimensional mixer, and mixing the mixture for 15-25 min at 25-35 r/min to obtain the targeted multi-mechanism synergistic anti-aging natural plant extract-nicotinamide composite preparation.
  9. 9. The use of the targeted multi-mechanism synergistic anti-aging natural plant extract-nicotinamide composite preparation according to any one of claims 1-8 in the preparation of functional foods or health care products for resisting aging, resisting oxidization and repairing DNA damage.
  10. 10. The application of the natural plant extract-nicotinamide composite preparation according to claim 9, wherein the functional food and the health-care product are powder, tablets or capsules, and the daily intake of the targeted multi-mechanism synergistic anti-aging natural plant extract-nicotinamide composite preparation is 1-1.5 g for adults.

Description

Natural plant extract-nicotinamide composite preparation with targeted multi-mechanism synergistic anti-aging function and preparation method and application thereof Technical Field The invention belongs to the technical field of application of natural active ingredients, and particularly relates to a targeted multi-mechanism synergistic anti-aging natural plant extract-nicotinamide composite preparation, and a preparation method and application thereof. The compound preparation can exert anti-aging effect by repairing damaged DNA cells, enhancing oxidation resistance of cells, and the like, and is suitable for preparing functional food, health products, and the like. Background Aging is a natural process of gradual decline of physiological functions of organisms over time, and the core mechanism of the aging is closely related to cell injury accumulation, increase of DNA mutation rate, oxidative stress unbalance and energy metabolism disturbance. Along with the aging increase of the global population, the demands of anti-aging related products continue to increase, but the prior art still has a plurality of limitations that on one hand, chemical synthesis anti-aging components (such as tretinoin and coenzyme Q10 derivatives) can improve the skin state in a short term, but have the problem of strong irritation and easy cytotoxicity after long-term use, and on the other hand, single natural components (such as grape seed extract and vitamin E) have single acting targets, so that the multi-dimensional aging problems of DNA damage repair, oxidative stress inhibition and the like are difficult to solve simultaneously. DNA mutation is a key driving factor of the aging process, particularly in the replication process of cells, the cells are influenced by factors such as ultraviolet rays, free radicals, environmental toxins and the like, DNA chains are easy to be damaged such as single-strand breakage, base mismatch and the like, if the repair is not timely, mutation rate can be gradually increased, and thus abnormal cell functions, aging acceleration and even disease induction are caused. It is worth noting that DNA mutation accumulation is closely related to tumorigenesis and development, namely, DNA repair capacity is reduced due to aging and oxidative stress is aggravated, so that the probability of activation of protooncogenes or inactivation of cancer suppressor genes is remarkably increased, and the DNA mutation accumulation becomes an important cause of tumorigenesis. The mutation frequency of human peripheral blood lymphocytes is in a stable rising trend along with the increase of age, the somatic mutation number of the elderly population is obviously increased compared with that of the young population, the number of the somatic mutations is increased from less than 500 mutations per cell of a neonate to more than 3000 per cell of a hundred year old, and the overall increase accords with the aging-related mutation accumulation rule. In addition, reactive Oxygen Species (ROS) generated by oxidative stress further exacerbate DNA damage, forming a vicious circle of "oxidative damage-mutation accumulation-aging acceleration". In the existing natural anti-aging components, the Rosa roxburghii extract is rich in vitamin C (the content can reach more than 2000mg/100 g), has certain anti-oxidation capability, but has poor stability of vitamin C alone and is easy to be oxidized and inactivated, anthocyanin in the blueberry extract can clear ROS but lacks the effect of directly repairing DNA damage, selenium is used as a key component of glutathione peroxidase, can regulate the redox balance of cells, but is low in biological utilization rate of selenium alone and is easy to cause toxic reaction, plantago ovata husk powder is rich in soluble dietary fibers, can indirectly improve organism metabolism by regulating intestinal microecology and can not directly act on an aging mechanism at a cell level, nicotinamide (vitamin B3) is used as a key precursor of NAD + synthesis, can be converted into NAD + through in vivo enzymatic reaction, so that Sirtuins family deacetylase is activated, DNA damage repair is promoted, oral absorbability is good, safety is clear, meanwhile, a DNA repair pathway on which is dependent on NAD + can reduce the occurrence risk of aging-related tumors through clearing mutant cells and inhibiting abnormal proliferation, but the conversion efficiency of the single nicotinamide is difficult to realize the synergistic effect with the anti-aging effect due to the fact that the conversion efficiency of NAD + is difficult to realize. Therefore, a compound formula based on natural plant extracts and nicotinamide (vitamin B3) is developed, the synergistic effect of DNA damage repair-oxidative stress inhibition-energy metabolism regulation is realized through scientific composition, and meanwhile, the problems of poor stability, low bioavailability and single efficacy target point of single component a