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CN-121986938-A - Nervonic acid functional composition for improving memory function and preparation method thereof

CN121986938ACN 121986938 ACN121986938 ACN 121986938ACN-121986938-A

Abstract

The invention discloses a nervonic acid functional composition for improving memory function and a preparation method thereof, which relate to the technical field of biomedical and functional food nutrition engineering, and the composition comprises the following raw materials in parts by weight: the three-dimensional compact microsphere wall material of the invention is constructed by catalytic cross-linking of transglutaminase, and the cross-linked network generates compact physical shrinkage in the strong acid gastric juice environment of a human body, extremely reduces the porosity, blocks the osmotic degradation of gastric acid and pepsin to internal micro-emulsion, and generates controlled swelling along with the dissociation of carboxyl when the composition is emptied into small intestine alkaline and medium containing pancreatin.

Inventors

  • JIA HAILIANG
  • ZHU BINGTAO
  • LI YULIANG

Assignees

  • 脑忆康(河北省)生物科技有限责任公司

Dates

Publication Date
20260508
Application Date
20260402

Claims (10)

  1. 1. The nervonic acid functional composition for improving memory is characterized by comprising the following raw materials in parts by weight: The core phase component comprises 15-30 parts of nervonic acid, 5-12 parts of phosphatidylserine, 3-8 parts of Bacopa monnieri extract, 0.5-2 parts of natural vitamin E and 10-20 parts of medium chain triglyceride; the wall material phase comprises 25-45 parts of sodium caseinate, 15-25 parts of propylene glycol alginate, 2-6 parts of ferulic acid and 5-10 parts of fructo-oligosaccharide; 0.1-0.5 part of functional auxiliary agent of transglutaminase; The composition is in a microcapsule structure, and the core phase component is embedded in a covalent cross-linked three-dimensional reticular shell layer formed by catalysis of transglutaminase through sodium caseinate, propylene glycol alginate and ferulic acid in the form of nanoemulsion.
  2. 2. The memory function-improving composition according to claim 1, wherein the purity of the nervonic acid is not less than 90%.
  3. 3. The memory function-improving composition according to claim 1, wherein the total mass fraction of bacoside a and bacoside B in the bacopa extract is not less than 20%.
  4. 4. The composition for improving memory according to claim 1, wherein the core phase comprises, by weight, 20 parts of nervonic acid, 8 parts of phosphatidylserine, 5 parts of Bacopa monnieri extract, 1 part of natural vitamin E, and 15 parts of medium chain triglyceride, wherein the wall phase comprises 35 parts of sodium caseinate, 20 parts of propylene glycol alginate, 4 parts of ferulic acid, 8 parts of fructo-oligosaccharides, and 0.3 part of transglutaminase.
  5. 5. The memory function-improving composition according to claim 1, wherein the nanoemulsion has a particle size of 150 to 300 nm.
  6. 6. A method for preparing the memory function-improving composition according to any one of claims 1 to 5, comprising the steps of: Heating medium chain triglyceride to 50-60 ℃, sequentially adding nervonic acid, natural vitamin E, phosphatidylserine and Bacopa monnieri extract into the medium chain triglyceride under the environment of continuous nitrogen protection, shearing and dispersing at high speed, adding deionized water with the same volume, and homogenizing under high pressure to obtain the primary core nanoemulsion; Preparing an active wall material composite aqueous phase solution, namely dispersing sodium caseinate and propylene glycol alginate in deionized water for hydration, then adding dissolved ferulic acid and fructo-oligosaccharide into the solution, and carrying out ultrasonic treatment to obtain a precursor co-coagulation network solution; Dripping the primary core nanoemulsion into the precursor co-coagulation network liquid at a constant speed, maintaining mechanical stirring, regulating the pH value of a system to 6.0-6.5 by using a citric acid solution, adding transglutaminase to perform enzymatic crosslinking reaction, and heating to inactivate the transglutaminase after the reaction is finished to obtain a recombinant crosslinked microsphere suspension; And (3) freeze drying and solidification forming, namely pre-freezing the recombinant crosslinked microsphere suspension, performing vacuum freeze sublimation drying, crushing and sieving the dried material to obtain the nervonic acid functional composition for improving the memory function.
  7. 7. The method according to claim 6, wherein in the step of preparing the primary core nanoemulsion, the primary core nanoemulsion is sheared for 10 to 15 minutes by a high-speed shearing and dispersing machine at a rotating speed of 8000 to 12000 r/min, and circularly homogenized for 3 to 5 times under a pressure of 40 to 60 MPa by a high-pressure homogenizer.
  8. 8. The method according to claim 6, wherein in the step of preparing the aqueous solution of the active wall material composite, sodium caseinate and propylene glycol alginate are dispersed in deionized water 10-15 times of the total mass of the aqueous solution, and the aqueous solution is continuously stirred for 2-3 hours at 45-55 ℃ until the aqueous solution is completely hydrated, and the aqueous solution is intermittently subjected to ultrasonic treatment for 15-20 minutes at 300-500W by using an ultrasonic cell grinder.
  9. 9. The preparation method according to claim 6, wherein in the step of composite embedding and enzymatic covalent crosslinking, the primary core nanoemulsion is dripped into the precursor co-coagulation network liquid at a rate of 2-4 mL/min, the mechanical stirring rate is 1000-1500 r/min, the pH value of the system is regulated by using a citric acid solution with a concentration of 0.1 mol/L, the enzymatic crosslinking reaction is carried out for 3-4 hours under the condition of avoiding light and continuously shaking slowly at 40 ℃, and the temperature of the system is raised to 85 ℃ and kept for 15 minutes after the reaction is finished so as to realize irreversible inactivation of transglutaminase.
  10. 10. The method according to claim 6, wherein in the steps of freeze-drying and solidification molding, the recombinant crosslinked microsphere suspension is pre-frozen below-40 ℃ for 4 hours, freeze-sublimation drying is performed for 36-48 hours under the conditions that the vacuum degree is less than 20 Pa and the cold trap temperature is less than-60 ℃, and the dried material is subjected to superfine grinding and sieving by a 100-mesh sieve.

Description

Nervonic acid functional composition for improving memory function and preparation method thereof Technical Field The invention relates to the technical field of biological medicine and functional food nutrition engineering, in particular to a nervonic acid functional composition for improving memory function and a preparation method thereof. Background With the continuous aggravation of the aging process of the population of the world, the occurrence rate of degenerative diseases of the central nervous system with hypomnesis and cognitive dysfunction as main clinical manifestations is in a trend of rising year by year, the advanced neural activities of the human brain, including normal operation of functions such as learning, memory and cognition, are highly dependent on normal conduction of neuron synapses in the central nervous system and the integrity of nerve fiber myelin, and research proves that the shedding, degeneration or damage of the nerve fiber myelin is one of the core pathological bases which cause the obstruction of memory conduction paths and the attenuation of information, and the nervonic acid (Nervonic Acid, cis-15-tetracosenic acid) is taken as a core marker component of a naturally occurring omega-9 long-chain monounsaturated fatty acid of the nerve myelin in the nerve tissues of mammals, and can directly participate in the self-repair and regeneration of the myelin of nerve fibers, and can also effectively clear necrotic microvascular lipid, so that the growth and branching of the axon are induced, however, the capacity of synthesizing the nervonic acid by the human body is extremely weak, and the main uptake of the nervonic acid becomes a key nutrition supplement strategy for improving and preventing the exogenous memory through the improvement of the cognitive dysfunction; Although the nervonic acid has irreplaceable physiological and pharmacological activity in the aspect of improving memory, the nervonic acid has obvious physicochemical and biological defects in the actual industrial application and the organism absorption and transformation process, firstly, the nervonic acid has an ultra-long carbon chain and an unsaturated double bond which is extremely easy to attack by free radicals in the molecular structure, so that the nervonic acid shows extremely strong water insolubility and extremely poor oxidation stability, and is extremely easy to be subjected to peroxidation degradation in the conventional processing and storage processes; secondly, because of the strong hydrophobicity, the nervonic acid is extremely difficult to be emulsified and dispersed in the gastrointestinal tract water-phase digestive juice, so that the trans-membrane absorption and utilization rate of the nervonic acid in small intestine microvilli epithelial cells is extremely low; In order to overcome the above-mentioned inherent defects of the nervonic acid, for example, the prior art patent document CN 117918425B (authorized publication number) discloses a "nervonic acid composition for improving memory learning function and a preparation method thereof", which aims to mix nutritional components such as nervonic acid, DHA, arachidonic acid and the like by adding lysolecithin and resistant dextrin and performing simple physical emulsification and homogenization, and further vacuum concentration or spray drying to prepare a composition, so as to improve the stability and bioavailability of the functional components in the composition, although the prior art delays the oxidation of grease during normal temperature storage to a certain extent, through intensive analysis and verification, the technology still has serious inherent defects, on the one hand, the "lysolecithin and resistant dextrin" adopted by the comparative document wrap active molecules such as nervonic acid only through physical adsorption and simple emulsification, and the like, the simple non-covalent microcosmic conformation is extremely easy to phase separation and internal phase disruption under the cooperation of gastric acid (pH 1.0-2.0) and pepsin and mechanical peristalsis, so that the internal phase separation and rapid internal phase disruption occur in the degradation medium containing the naked nervonic acid is not degraded by the reverse degradation of free radical in gastric juice; on the other hand, the loose microstructure constructed based on simple polysaccharide and phospholipid lacks the capability of precisely targeting and slowly releasing control on specific digestive tract parts, so that the active components do not enter the main absorption organ, namely small intestine parts, are greatly lost, and finally the technical bottlenecks of low bioavailability of nervonic acid and lacking deep physiological synergy among multiple components are not fundamentally solved. Disclosure of Invention In order to overcome the technical defects that active ingredients such as nervonic acid and the like are easy to be destroyed in