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CN-121987379-A - Animal model construction method for researching jaw cyst and application thereof

CN121987379ACN 121987379 ACN121987379 ACN 121987379ACN-121987379-A

Abstract

The invention relates to the technical field of medical experimental animal models, in particular to an animal model construction method for researching a jaw cyst, which comprises the steps of selecting an experimental animal, preparing an operation part, performing bone windowing operation, implanting a cyst induction material, suturing a wound, nursing and observing after operation and simulating the development of the jaw cyst in vivo by using an implant. The invention provides the animal model of the jaw cyst, which has high modeling success rate, good stability, high similarity with pathological characteristics of a bone cavity of the jaw cyst of a human being and simple operation, and the construction method thereof, and provides a reliable experimental model for research of pathogenesis of the jaw cyst, study of pathological process, research and development of medicines, evaluation of treatment methods and the like.

Inventors

  • LI MENGHAN
  • LIU FAYU
  • CHEN ZHIQING
  • ZHAO LIN
  • ZHANG AIHUA

Assignees

  • 中国医科大学附属口腔医院

Dates

Publication Date
20260508
Application Date
20260121

Claims (10)

  1. 1. An animal model construction method for researching a jaw cyst, which is characterized by comprising the following steps: (1) Selecting healthy animals with proper age and weight as experimental animals; (2) Preparing an operation part, namely performing general anesthesia on an experimental animal, and performing conventional disinfection and towel spreading on the oromaxillofacial region after the anesthesia is successful, so as to fully expose an operation area; (3) Bone windowing operation, namely preparing a buccal bone window with proper size on the buccal side of the mandible body of an experimental animal by using a low-speed dental handpiece in combination with a bone drill, wherein the depth of the bone window is proper to penetrate through cortical bone to reach cancellous bone, and important blood vessels and nerves are required to be prevented from being damaged in the operation process; (4) Implanting a cyst inducing material, namely fixing and implanting the cyst inducing material into a buccal bone window; (5) Wound suturing, namely tightly suturing an operation wound after the cyst inducing material is implanted, ensuring good wound closure and preventing the induction tube from falling off, the induction tube from being damaged and infection after operation; (6) Post-operation nursing and observation, namely placing experimental animals in a separate feeding cage after operation, administering antibiotics to prevent infection, and continuously injecting for 3 days; (7) The implant material simulates the development of the jaw cyst in vivo, three inflammatory factors IL-6, IL-1 beta and PGE2 with highest expression are detected in a bone cavity by using jaw cyst fluid elisa, chronic inflammatory changes are simulated for 3 or 6 weeks, then imaging examination is carried out on experimental animals every week, and the formation and development conditions of the jaw cyst are observed, so that the prepared jaw cyst animal model is obtained.
  2. 2. The method for constructing an animal model for studying a jaw cyst of claim 1, wherein the experimental animal in step (1) is adaptively raised in advance for one week, during which the health condition of the animal is observed, and the animal is ensured to have no disease infection, and the diet and activity physiological indexes are normal.
  3. 3. The method of claim 1, wherein the cyst inducing material in step (4) is an expandable channel tube or an expandable hydrogel material containing a slow-release induced inflammatory factor.
  4. 4. The method according to claim 3, wherein when the cyst inducing material is an expandable channel tube in the step (4), the step (7) of injecting an inducer and simulating the change of the expansibility is performed by injecting physiological saline from the inlet A of the material to expand the end of the bone inner tube 3 days after the tube is implanted, simulating the change of the expansibility 1 time a day, 0.05-0.2ml each time, continuously injecting pro-inflammatory factors IL-6, IL-1 beta and PGE 21 time a week from the inlet B for 3 or 6 weeks, injecting IL-6 for the first week, injecting IL-1 beta for the second week, injecting PGE2 for the third week, repeating the operations for the first, second and third weeks, and releasing inflammatory factors from the end of the tube.
  5. 5. The method of claim 3, wherein when the cyst inducing material in the step (4) is a swelling hydrogel material containing a slow-release inflammatory factor, the step (7) of slowly releasing the inducer and simulating swelling change is performed by releasing the pro-inflammatory factor in bone after the swelling hydrogel material containing the inflammatory factor is implanted, and simulating swelling change of the cyst by swelling in the hydrogel bone for 3 or 6 weeks.
  6. 6. The method of constructing an animal model for studying a jaw cyst according to claim 1, wherein said suturing in step (5) is performed using absorbable suture.
  7. 7. The method of constructing an animal model for studying a jaw cyst of claim 4, wherein said inlet A and said inlet B are each provided with positive pressure means.
  8. 8. The method of claim 1, wherein the imaging of step (7) includes but is not limited to X-ray or CT.
  9. 9. The method according to claim 1, wherein the animal in the step (1) is a rabbit or SD rat, the anesthetic used in the anesthesia in the step (2) is uratam for rabbit or avermectin for mouse, and the antibiotic used in the step (6) is penicillin sodium at a dose of 3-6 ten thousand U/kg.
  10. 10. The method of claim 1, wherein the model is used for pathogenesis studies, drug screening or therapy assessment.

Description

Animal model construction method for researching jaw cyst and application thereof Technical Field The invention relates to the technical field of medical experimental animal models, in particular to an animal model construction method for researching jaw cyst and application thereof. Background Jaw cysts are one of the most common diseases of the oromaxillofacial region, including odontogenic and non-odontogenic jaw cysts, and research on pathogenesis, pathological processes and treatment methods thereof has been an important subject in the field of stomatology. For many years, the first treatment strategy for jaw cysts has been surgical scraping, however whatever surgical procedure leaves empty bone cavities after surgery. For the cavity with smaller volume, the body can heal by using clot organization without treatment. For lesions with larger volume, research has found that even if the cavity volume is reduced by using methods such as windowing and depressurizing, self-complete healing cannot be realized. When the bone defect exceeds a certain range, the organism can not repair by oneself, namely critical bone defect, the oversized bone defect can influence tissue regeneration and functional reconstruction, can not realize bone healing, influence stress distribution, even lead to jawbone fracture, influence the physical and mental health of a patient. How to promote accurate and efficient regeneration of the bone cavity after cyst operation is a key problem to be solved in the oral clinic field. Developing an effective preclinical study is a prerequisite for screening viable therapeutic regimens, which in turn depends on whether or not an appropriate animal model is employed. At present, a simple mechanical bone defect model is mostly adopted in preclinical research of bone cavity healing after jaw cyst operation. Such models are widely used because of their standardization, but their physiological and pathological background is far from the state after a jawbone cyst operation. The effective bone regeneration strategy in a simple model does not necessarily have the same efficacy in complex clinical cyst bone cavities. In the past, animal experiments for inducing the jaw cyst mainly adopt 3 methods, namely, epithelial implantation, tooth embryo transplantation and exposed dental pulp cavity. The related scholars try to implant gingival papilla, palate mucosa and the like into the jawbone of a rat or a monkey so as to successfully induce the generation of the odontogenic jaw cyst, however, the observation time of the induction method is variable from 45 days to 364 days, the observed time is less similar to that of the known odontogenic cyst in morphology, the success rate is lower, and the odontogenic jaw cyst cannot be stably induced. In addition, multiple scholars implant donor dental embryos into subcutaneous, renal submucosal, fat pad or cheek pouch of allogenic animals after separating the donor dental embryos from the jaw bone of a new animal, explore the possibility of clinical application of allogenic dental embryo transplantation, and although animal cysts induced by the induction method seem to have some similarities with human dental cysts, only the odontogenic epithelium can be proved to generate keratinization, long-term growth and behavior characteristics of the odontoembryo cannot be judged due to limited observation time after the dental embryo transplantation, and no evidence exists that the method can successfully induce the cyst generation in the jaw bone. There are also scholars attempting pulp removal and root canal preparation for enlargement beyond the root tip on the first molar of the mandible of rats, opening the root canal orifice to allow continuous contamination of oral bacteria to induce root tip cysts by exposing the pulp chamber, and after 6-8 months, only 1/7 of the success in inducing cyst structure was found, and subsequent studies have also found that this method mostly induced root tip Zhou Rouya swelling in periapical area, not all root tip Zhou Rouya swelling to be converted into cysts, and the formation of cysts may depend on various factors such as the existence of epithelial residues, the type and intensity of inflammation, and individual differences of hosts, and thus cannot be a widely used animal model of jawbone cyst. The above methods for inducing cyst animal models cannot be used for inducing jawbone cysts stably in animals. The specific biological microenvironment that the peripheral wall of the bone cavity is bone tissue chronically infiltrated by chronic inflammation of the cyst, and the inherent cell activity and factor expression profile are quite different from those of the bone wall caused by acute mechanical trauma (such as drilling) cannot be simultaneously satisfied in a short period of time. (2) The dynamically formed lacunae structure, cysts form bone cavities through slow expansion and compression absorption, which shapes the unique morphologic