CN-121987505-A - Preparation method and application of collagen peptide-loaded stratum corneum biomimetic nanoliposome

Abstract

The invention discloses a preparation method and application of a collagen peptide-loaded stratum corneum biomimetic nano liposome, and belongs to the technical field of polypeptide preparation. The preparation method comprises the steps of heating and dissolving hydrogenated lecithin, ceramide, cholesterol and dipropylene glycol, adding collagen peptide aqueous solution, hydrating and stirring at 60-100 ℃, carrying out high-speed shearing and cooling on the obtained lipid mixture, carrying out circulating homogenization on the lipid mixture at 40-160 MPa by a high-pressure micro-jet homogenizer to obtain nano liposome emulsion, mixing the emulsion with polyethylene glycol 400-8000, standing in an ice bath, centrifuging, discarding supernatant, redissolving the purified liposome, and carrying out freezing or spray drying to obtain solid powder. The invention eliminates the traditional steps of rotary steaming and the like, has simple flow and is easy to realize industrial production, and the prepared nano liposome remarkably improves the transdermal effect of collagen peptide and has wide application prospect in the field of functional skin care.

Inventors

• WU HAOHAO
• LIU CHENGSUO
• QIU DAN
• ZHAO ZIFANG
• GUO HONGXING
• QI XINYUAN

Assignees

• 中国海洋大学三亚海洋研究院
• 海南华研胶原科技股份有限公司

Dates

Publication Date

20260508

Application Date

20260213

Claims (4)

1. 1. The preparation method of the collagen peptide-loaded stratum corneum biomimetic nanoliposome is characterized by comprising the following steps of: (1) Mixing hydrogenated lecithin, ceramide, cholesterol and dipropylene glycol according to different proportions, and heating and dissolving at 60-100 ℃ to prepare a uniform and transparent lipid mixture with a solid-liquid ratio of 2:3; (2) Preparing a collagen peptide aqueous solution with the mass concentration of 5% -30%, slowly adding the collagen peptide aqueous solution into a lipid mixture, hydrating and stirring the mixture at 60-100 ℃ for 15min, and then homogenizing the mixture at the temperature at a rotating speed of 8000-15000-rpm for 2-5 min at a high speed to obtain an emulsion with uniform texture; (3) After the emulsion is cooled to room temperature rapidly, introducing the emulsion into a high-pressure micro-jet homogenizer, and circularly homogenizing for 1 to 5 times under the condition of 40 Mpa to 160 Mpa to form nano liposome emulsion; (4) Mixing polyethylene glycol 400-8000 solution with the mass concentration of 10% -25% with nano liposome emulsion according to the volume ratio of 1:2-3:1, standing in ice bath for 30 min, centrifuging at 500-4000 Xg for 5-20: 20min, discarding supernatant, redissolving the precipitated liposome in water, and freeze-drying or spray-drying to obtain nano liposome powder.
2. 2. The method of claim 1, wherein the ratio of dipropylene glycol to total lipid in step (1) is 3:2 (w/w).
3. 3. The preparation method according to claim 1, wherein the ratio of lipid substances in the step (1) is 5:1 to 1:2 (w/w) by mass of collagen peptide to hydrogenated lecithin, 20:1 to 10:3 (w/w) by mass of hydrogenated phospholipid to cholesterol, and 20:1 to 5:2 (w/w) by mass of hydrogenated phospholipid to ceramide.
4. 4. Use of the biomimetic nanoliposomes of the stratum corneum obtainable by the process according to any one of the preceding claims for the production of functional skin care products.

Description

Preparation method and application of collagen peptide-loaded stratum corneum biomimetic nanoliposome Technical Field The invention relates to a preparation method and application of a collagen peptide-loaded stratum corneum biomimetic nano liposome, belonging to the field of biological product processing. Background The collagen peptide is bioactive polypeptide prepared by taking animal connective tissue as a raw material and adopting an enzymolysis process. Collagen peptide stimulates fibroblast to synthesize new collagen and elastin through signal transduction, and simultaneously plays the roles of moisturizing, anti-wrinkle and scavenging free radicals, thereby protecting skin. However, collagen peptides, due to their strong hydrophilicity, have difficulty penetrating the hydrophobic barrier of the outermost layer of the skin, the stratum corneum. The collagen peptide has extremely low skin bioavailability, and limits the application potential in functional skin care products. The skin serves as the largest organ of the human body and carries multiple physiological functions including barrier protection, sensory conduction, thermoregulation, immune defenses and the like. The skin is composed of epidermis, dermis and subcutaneous tissue, and the stratum corneum, which is the outermost layer of the epidermis, is the core permeation barrier, and its unique lipid composition and structural features directly regulate the transdermal behavior of exogenous substances. The intercellular lipid of the horny layer mainly comprises ceramide (40% -50%), cholesterol (25% -30%) and free fatty acid (15% -25%) which are arranged in a specific proportion to form a layered structure, so that a main channel for transdermal diffusion of substances is formed. Wherein ceramide serves as a barrier "backbone" to reduce percutaneous water loss and inhibit inflammation, and cholesterol stabilizes lipid bilayer by regulating lipid fluidity. The liposome has a phospholipid bilayer structure similar to a biological membrane, can encapsulate hydrophilic active substances (such as collagen peptide), and can realize deep delivery of skin through membrane fusion, hair follicle transportation, permeation promotion and other ways. However, the existing commercial liposome products still face multiple technical bottlenecks, namely common lecithin rich in unsaturated fatty acid is easy to oxidize, the product stability is poor, the shelf life is short, the stratum corneum lipid bionic design is lacked, the skin permeability is low, the traditional process flows of a film dispersion method and the like are complex, the cost is high, and the large-scale production is difficult. Therefore, developing a collagen peptide nanoliposome with bionic components, excellent stability and simple process flow has become a key technical problem to be solved urgently in the field at present. Disclosure of Invention The invention aims to provide a preparation method and application of collagen peptide-loaded stratum corneum biomimetic nano-liposome. The method aims to solve the problems that the existing collagen peptide has poor percutaneous absorption and the preparation process is difficult to industrialize. The technical scheme adopted for realizing the purpose of the invention is as follows: the preparation method of the collagen peptide-bionic skin nano liposome comprises the following steps: (1) Mixing hydrogenated lecithin, cholesterol, ceramide and dipropylene glycol in proportion, heating and dissolving at 60-90 ℃ to form a uniform lipid mixture, slowly adding a collagen peptide aqueous solution preheated to the same temperature into the lipid mixture, stirring and hydrating at a constant temperature, and carrying out high-speed shearing and homogenizing on the mixed solution to obtain an emulsion with uniform texture. The ratio of dipropylene glycol to total lipid is 3:2 (w/w), the preferable ratio of lipid substances is that the mass ratio of collagen peptide to hydrogenated lecithin is 5:1-1:2 (w/w), the mass ratio of hydrogenated phospholipid to cholesterol is 20:1-10:3 (w/w), and the mass ratio of hydrogenated phospholipid to ceramide is 20:1-5:2 (w/w). (2) After the colostrum is cooled to room temperature, introducing the emulsion into a high-pressure micro-jet homogenizer, and circularly homogenizing for 1-5 times under the condition that the homogenizing pressure is 40 Mpa-160 Mpa to obtain the nano liposome emulsion (CPL). (3) Mixing the nano liposome emulsion with polyethylene glycol (PEG) 400-8000 with the mass concentration of 10% -25%, standing in an ice bath for 30-min%, centrifuging for 5-20 min under the condition of 500-4000 Xg, discarding the supernatant to remove unencapsulated free peptide, and collecting the precipitate to obtain the high-purity nano liposome; (4) Adding water into the collected nano liposome for re-dissolution, and carrying out freeze drying or spray drying treatment to obtain a nano liposome powder product.